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21. 发明授权

US07273704B2 Method of detecting nucleic acid by using DNA microarrays and nucleic acid detection apparatus 有权
标题翻译：使用DNA微阵列和核酸检测装置检测核酸的方法
公开(公告)号：US07273704B2
公开(公告)日：2007-09-25
申请号：US10522991
申请日：2002-08-12
申请人： Takuya Matsui , Yuji Miyahara , Kumiko Hattori
发明人： Takuya Matsui , Yuji Miyahara , Kumiko Hattori
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6825 , B01J2219/00286 , B01J2219/00497 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00653 , B01J2219/00662 , B01J2219/00677 , B01J2219/00689 , B01J2219/00722 , C12Q1/6837 , G01N27/4145 , G01N33/54373 , C12Q2565/607 , C12Q2565/501 , C12Q2545/114
摘要： A nucleic acid contained in a sample is quantified with high accuracy. The procedure includes the step of allowing the sample containing a specific nucleic acid to interact with a DNA microarray provided with a plurality of nucleic acid probe parts having a nucleic acid probe capable of hybridizing to the specific nucleic acid; the step of monitoring outputs from each of the plurality of nucleic acid probe parts due to hybridization between the nucleic acid probe and the specific nucleic acid to determine normal distribution of time taken to reach a predetermined output value with respect to each of the plurality of nucleic acid probe parts; and the step of quantifying the specific nucleic acid contained in the sample based on a maximum value determined from the normal distribution obtained in the above step.
摘要翻译：以高精度量化样品中所含的核酸。该方法包括允许含有特定核酸的样品与提供有多个具有能够与特定核酸杂交的核酸探针的多个核酸探针部分的DNA微阵列相互作用的步骤; 由于核酸探针和特定核酸之间的杂交，监测来自多个核酸探针部分中的每一个的输出的步骤，以确定相对于多个核酸中的每一个核酸达到预定输出值所需的时间的正态分布酸性探针部件; 以及基于从上述步骤中获得的正态分布确定的最大值来量化样品中所含特定核酸的步骤。

22. 发明授权

US07273702B2 Apparatus for purifying nucleic acid and method of purifying nucleic acid 有权
标题翻译：核酸纯化装置及核酸纯化方法
公开(公告)号：US07273702B2
公开(公告)日：2007-09-25
申请号：US10478096
申请日：2002-05-24
申请人： Teruhisa Akashi , Yukiko Ikeda , Yoshihiro Nagaoka , Naruo Watanabe , Yuji Miyahara
发明人： Teruhisa Akashi , Yukiko Ikeda , Yoshihiro Nagaoka , Naruo Watanabe , Yuji Miyahara
IPC分类号： C12Q1/68 , C12M1/00 , C12M3/00 , C07H21/02
CPC分类号： B01L3/50273 , B01L3/5025 , B01L3/502738 , B01L3/502753 , B01L2200/027 , B01L2300/0803 , B01L2300/0867 , B01L2300/1827 , B01L2400/0409 , B01L2400/0694 , G01N35/00069 , G01N2030/381
摘要： A nucleic acid refining apparatus, being ease in automation thereof, keeping high in contacting frequency between nucleic acid within a sample and a solid phase during nucleic acid capture processing, thereby proving high capturing rate, comprises: means for separating a liquid containing the nucleic acid therein from said sample through centrifugal force; means for transferring a reagent through the centrifugal force; means for producing a mixture liquid of said reagent transferred through the centrifugal force and a solution containing said nucleic acid therein; a carrier for capturing said nucleic acid; means for transferring said mixture liquid to said carrier through the centrifugal force; heating means for heating said carrier; and a holding means for separating and holding the reagent containing said nucleic acid eluting from said carrier, separating from other reagent, through different centrifugal.
摘要翻译：一种核酸精制装置，其易于自动化，在核酸捕获处理期间保持样品内的核酸与固相之间的接触频率高，从而证明高捕获率，包括：用于分离含有核酸的液体的装置其中从所述样品通过离心力; 用于通过离心力转移试剂的装置; 用于生产通过离心力转移的所述试剂的混合液体的装置和其中含有所述核酸的溶液; 用于捕获所述核酸的载体; 用于通过离心力将所述混合液转移到所述载体的装置; 用于加热所述载体的加热装置; 以及用于分离并保持含有从所述载体洗脱的所述核酸的试剂的保持装置，通过不同的离心与其它试剂分离。

23. 发明申请

US20060088839A1 Method of detecting nucleic acid by using dna microarrays and nucleic acid detection apparatus 有权
标题翻译：通过使用dna微阵列和核酸检测装置检测核酸的方法
公开(公告)号：US20060088839A1
公开(公告)日：2006-04-27
申请号：US10522991
申请日：2002-08-12
申请人： Takuya Matsui , Yuji Miyahara , Kumiko Hattori
发明人： Takuya Matsui , Yuji Miyahara , Kumiko Hattori
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： C12Q1/6825 , B01J2219/00286 , B01J2219/00497 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00653 , B01J2219/00662 , B01J2219/00677 , B01J2219/00689 , B01J2219/00722 , C12Q1/6837 , G01N27/4145 , G01N33/54373 , C12Q2565/607 , C12Q2565/501 , C12Q2545/114
摘要： A nucleic acid contained in a sample is quantified with high accuracy. The procedure includes the step of allowing the sample containing a specific nucleic acid to interact with a DNA microarray provided with a plurality of nucleic acid probe parts having a nucleic acid probe capable of hybridizing to the specific nucleic acid; the step of monitoring outputs from each of the plurality of nucleic acid probe parts due to hybridization between the nucleic acid probe and the specific nucleic acid to determine normal distribution of time taken to reach a predetermined output value with respect to each of the plurality of nucleic acid probe parts; and the step of quantifying the specific nucleic acid contained in the sample based on a maximum value determined from the normal distribution obtained in the above step.
摘要翻译：以高精度量化样品中所含的核酸。该方法包括允许含有特定核酸的样品与提供有多个具有能够与特定核酸杂交的核酸探针的多个核酸探针部分的DNA微阵列相互作用的步骤; 由于核酸探针和特定核酸之间的杂交，监测来自多个核酸探针部分中的每一个的输出的步骤，以确定相对于多个核酸中的每一个核酸达到预定输出值所需的时间的正态分布酸性探针部件; 以及基于从上述步骤中获得的正态分布确定的最大值来量化样品中所含特定核酸的步骤。

24. 发明授权

US06977149B2 Biochemical reaction detection apparatus 失效
标题翻译：生化反应检测仪
公开(公告)号：US06977149B2
公开(公告)日：2005-12-20
申请号：US10034075
申请日：2002-01-03
申请人： Tomoharu Kajiyama , Yuji Miyahara , Katsuji Murakawa
发明人： Tomoharu Kajiyama , Yuji Miyahara , Katsuji Murakawa
IPC分类号： B01J19/00 , B01L3/00 , B01L7/00 , C12Q1/68 , C40B40/06 , C40B60/14 , C12M1/00 , C12N11/16 , G01N15/06
CPC分类号： B01L7/52 , B01J19/0046 , B01J2219/00495 , B01J2219/00497 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00689 , B01J2219/00695 , B01J2219/00707 , B01J2219/00722 , B01L3/50851 , B01L7/54 , B01L2300/0636 , B01L2300/0819 , C12Q1/6837 , C40B40/06 , C40B60/14 , Y10S977/924 , C12Q2527/107
摘要： A biochemical reaction detection chip capable of controlling the temperature for biochemical reactions including hybridizations and its substrate. The function of the chip is performed by comprising a plurality of islands of a heat conducting material on the membrane of the substrate, the islands being spaced from each other and individually provided with temperature controllers, and the probes immobilized on the substrate.
摘要翻译：一种生化反应检测芯片，能够控制生化反应的温度，包括杂交及其底物。芯片的功能是通过在衬底的膜上包括多个导热材料的岛来实现的，这些岛彼此间隔开并且分别设置有温度控制器，并且探针固定在衬底上。

25. 发明授权

US06930198B2 Method for production of unsaturated carboxylic ester 有权
标题翻译：不饱和羧酸酯的制备方法
公开(公告)号：US06930198B2
公开(公告)日：2005-08-16
申请号：US10132529
申请日：2002-04-24
申请人： Kazuto Okazaki , Yukihiro Matsumoto , Kazuhiko Sakamoto , Yuji Miyahara
发明人： Kazuto Okazaki , Yukihiro Matsumoto , Kazuhiko Sakamoto , Yuji Miyahara
IPC分类号： C07B61/00 , C07C67/08 , C07C69/003 , C07C69/54 , C07C69/52
CPC分类号： C07C67/08 , C07C69/54
摘要： A method for producing an unsaturated carboxylic ester is provided, which can prevent the raw material compounds and the product of the reaction from splashing and adhering on the inner wall of the reaction vessel and succumbing to polymerization on the inner wall. Specifically, it is characterized by the fact that the difference between the outer periphery of the reaction vessel heated by the heating means and the reaction solution in temperature is not higher than 80° C.
摘要翻译：提供一种不饱和羧酸酯的制造方法，其可以防止原料化合物和反应产物飞溅并附着在反应容器的内壁上并在内壁上聚合。具体地说，其特征在于，由加热装置加热的反应容器的外周与反应溶液的温度之间的差异不高于80℃。

26. 发明申请

US20140124383A1 ELECTRODE CHIP FOR DETECTING BIOLOGICAL MOLECULE, AND METHOD FOR DETECTING BIOLOGICAL MOLECULE 审中-公开
标题翻译：用于检测生物分子的电极芯片和用于检测生物分子的方法
公开(公告)号：US20140124383A1
公开(公告)日：2014-05-08
申请号：US14112383
申请日：2012-04-20
申请人： Yuji Miyahara , Akira Matsumoto , Tatsuro Goda , Yasuhiro Maeda , Chiho Kataoka
发明人： Yuji Miyahara , Akira Matsumoto , Tatsuro Goda , Yasuhiro Maeda , Chiho Kataoka
IPC分类号： G01N27/327
CPC分类号： G01N27/3276 , G01N27/3277 , G01N27/4145
摘要： An electrode chip for detecting a biomolecule includes a substrate; and an electrode that includes an electrode substrate, at least one of a metallic salt or a metallic oxide provided on an outermost surface on an opposite side of the electrode substrate from a side provided with the substrate, and a biomolecular probe-immobilizing material which is provided on the outermost surface and to which a biomolecular probe is fixed.
摘要翻译：用于检测生物分子的电极芯片包括：基板; 以及电极，包括电极基板，设置在电极基板的与设置有基板的一侧相反的一侧的最外表面上的金属盐或金属氧化物中的至少一种，以及生物分子探针固定化材料，其为提供在最外表面上并且固定有生物分子探针。

27. 发明授权

US08668871B2 Sensing device and biosensor 有权
标题翻译：感应装置和生物传感器
公开(公告)号：US08668871B2
公开(公告)日：2014-03-11
申请号：US13513939
申请日：2011-07-20
申请人： Akira Matsumoto , Yuji Miyahara
发明人： Akira Matsumoto , Yuji Miyahara
IPC分类号： G01N33/53
CPC分类号： C07F5/025 , C07B2200/11 , G01N5/02 , G01N27/4145
摘要： Provided are a sensing device and a biosensor that are capable of achieving stable performance in sensing saccharides or hydroxyl group-containing polymers, and that can be stored for a long time. The biosensor 8 of the present invention uses, as a component for sensing glucose, a sensing device 16 in which a completely synthetic phenylboronic acid compound 21 is bound without using enzymes that have been conventionally used. Consequently, the biosensor 8 can eliminate the problem of protein denaturation to achieve stable performance in sensing glucose, and it is possible to achieve the biosensor 8 can be stored for a long time.
摘要翻译：提供能够在感测糖类或含羟基聚合物中获得稳定性能的感测装置和生物传感器，并且可以长期储存。本发明的生物传感器8使用传感装置16作为检测葡萄糖的组分，其中完全合成的苯基硼酸化合物21不经过常规使用的酶结合。因此，生物传感器8可以消除蛋白质变性的问题，以实现感测葡萄糖的稳定性能，并且可以实现生物传感器8的存储长时间。

28. 发明申请

US20050170347A1 Potentiometric dna microarray, process for producing the same and method of analyzing nucleic acid 审中-公开
标题翻译：电位滴定微阵列，其生产方法和分析核酸的方法
公开(公告)号：US20050170347A1
公开(公告)日：2005-08-04
申请号：US10499005
申请日：2001-12-19
申请人： Yuji Miyahara , Kenji Yasuda , Kumiko Hattori
发明人： Yuji Miyahara , Kenji Yasuda , Kumiko Hattori
IPC分类号： B01J19/00 , B01L3/00 , C12M1/00 , C12N15/09 , C12Q1/68 , C40B40/06 , G01N27/414 , H01L29/06 , H01L29/66 , C12M1/34
CPC分类号： B01J19/0046 , B01J2219/00529 , B01J2219/00585 , B01J2219/00596 , B01J2219/00608 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00653 , B01J2219/00659 , B01J2219/00722 , B01L3/5085 , C12Q1/6825 , C12Q1/6837 , C40B40/06 , G01N27/4145 , C12Q2565/501 , C12Q2565/607
摘要： A DNA microarray system whereby measurement can be performed at a low running cost, a low price and yet a high accuracy. A nucleic acid probe (3) is immobilized on the surface of a gate insulator of an electric field effect transistor and then hybridized with a target gene on the surface of the gate insulator. A change in the surface electric charge density thus arising is detected by using the electric effect.
摘要翻译：一种DNA微阵列系统，其可以以低运行成本，低价格和高精度进行测量。将核酸探针（3）固定在电场效应晶体管的栅极绝缘体的表面上，然后与栅极绝缘体的表面上的目标基因杂交。通过使用电效应来检测由此产生的表面电荷密度的变化。

29. 发明授权

US06841128B2 DNA base sequencing system 有权
标题翻译： DNA碱基测序系统
公开(公告)号：US06841128B2
公开(公告)日：2005-01-11
申请号：US09805240
申请日：2001-03-14
申请人： Hideki Kambara , Guohua Zhou , Yuji Miyahara
发明人： Hideki Kambara , Guohua Zhou , Yuji Miyahara
IPC分类号： G01N27/447 , C12M1/00 , C12M1/34 , C12N15/09 , C12Q1/68 , G01N30/02
CPC分类号： C12Q1/6869 , C12Q2565/537 , C12Q2565/301
摘要： The present invention provides a DNA base sequencing system having a compact, simple and convenient structure.In one embodiment of the present invention, a reaction chamber module for pyrosequencing in which a multiple number of reaction vessels (reaction chambers) 10 and reagent-introducing narrow tubes 6 are integrated is formed in a device board 5. Reagents held in reagent reservoirs 1, 2, 3 and 4 mounted separately from this reaction chamber module are introduced into the reaction vessels 10 via reagent-introducing narrow tubes (capillaries) 6. The reagent-introducing narrow tubes (capillaries) 6 at the area of 2 cm from the reaction vessels 10 are structured with narrow capillaries having an inner diameter of about 0.1 mm and the conductance of these capillaries for the reagent solution determines the injection speed of the solution.Using the present invention, many kinds of DNAs can be analyzed simultaneously.
摘要翻译：本发明提供了具有紧凑，简单和方便结构的DNA碱基测序系统。在本发明的一个实施方案中，用于焦磷酸测序的反应室模块，其中多个反应容器（反应室）10和试剂引入窄在装置基板5上形成有管6的集成体。保持在与该反应室模块分开设置的试剂容器1,2,3和4中的试剂通过试剂引入的窄管（毛细管）6被引入反应容器10。在距反应容器10的2cm区域处的试剂导入窄管（毛细管）6被构造成具有内径约0.1mm的窄毛细管，并且用于试剂溶液的这些毛细管的电导决定了溶液的注入速度使用本发明，可以同时分析多种DNA。

30. 发明授权

US06428749B1 Advanced thermal gradient DNA chip (ATGC), the substrate for ATGC, method for manufacturing for ATGC, method and apparatus for biochemical reaction, and storage medium 失效
公开(公告)号：US06428749B1
公开(公告)日：2002-08-06
申请号：US09527233
申请日：2000-03-16
申请人： Tomoharu Kajiyama , Yuji Miyahara , Katsuji Murakawa
发明人： Tomoharu Kajiyama , Yuji Miyahara , Katsuji Murakawa
IPC分类号： G01N1500
CPC分类号： B01L7/52 , B01J19/0046 , B01J2219/00495 , B01J2219/00497 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00689 , B01J2219/00695 , B01J2219/00707 , B01J2219/00722 , B01L3/50851 , B01L7/54 , B01L2300/0636 , B01L2300/0819 , C12Q1/6837 , C40B40/06 , C40B60/14 , Y10S977/924 , C12Q2527/107
摘要： A biochemical reaction detection chip capable of controlling the temperature for biochemical reactions including hybridizations and its substrate. The function of the chip is performed by comprising a plurality of islands of a heat conducting material on the membrane of the substrate, the islands being spaced from each other and individually provided with temperature controllers, and the probes immobilized on the substrate.

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