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    • 23. 发明申请
    • ASSAY TOOLS AND METHODS OF USE
    • 测定工具和使用方法
    • US20120129248A1
    • 2012-05-24
    • US13388229
    • 2010-08-02
    • Mark S. CheeIgor A. Kozlov
    • Mark S. CheeIgor A. Kozlov
    • G01N33/53C12M1/40
    • G01N33/54366C12Q1/34C12Q1/37G01N33/573
    • The present invention provides assay tools for the detection of biological or chemical activity in a sample. The assay tools of the invention provide direct detection using a positive signal generated on a surface of the assay tool. These assay tools provide improved methods for detection and/or identification of multiple agents (e.g., enzymes) in a sample, analysis of substrate specificity of such agents, and binding affinities and specificities of such agents. Upon activity a component is released from a first immobilised construct and then captured by a capture surface. At least two different immobilised constructs are used.
    • 本发明提供了用于检测样品中生物或化学活性的测定工具。 本发明的测定工具使用在测定工具的表面上产生的阳性信号提供直接检测。 这些测定工具提供了用于样品中多个试剂(例如酶)的检测和/或鉴定的改进方法,这些试剂的底物特异性的分析以及这些试剂的结合亲和力和特异性。 在活动时,将组分从第一固定化构建物释放,然后由捕获表面捕获。 使用至少两种不同的固定化构建体。
    • 24. 发明申请
    • METHODS OF ENZYMATIC DISCRIMINATION ENHANCEMENT AND SURFACE-BOUND DOUBLE-STRANDED DNA
    • 酶切鉴定增强和表面双重双链DNA的方法
    • US20100216656A1
    • 2010-08-26
    • US12187503
    • 2008-08-07
    • David J. LockhartMark S. CheeDirk VetterMartin Digglemann
    • David J. LockhartMark S. CheeDirk VetterMartin Digglemann
    • C40B30/04C40B60/12
    • C40B60/12B01J2219/00427B01J2219/00529B01J2219/00596B01J2219/00608B01J2219/00659B01J2219/00722C12Q1/6837C12Q1/6869C12Q2565/507C12Q2521/501
    • Methods for discriminating between fully complementary hybrids and those that differ by one or more base pairs and libraries of unimolecular, double-stranded oligonucleotides on a solid support. In one embodiment, the present invention provides methods of using nuclease treatment to improve the quality of hybridization signals on high density oligonucleotide arrays. In another embodiment, the present invention provides methods of using ligation reactions to improve the quality of hybridization signals on high density oligonucleotide arrays. In yet another embodiment, the present invention provides libraries of unimolecular or intermolecular, double-stranded oligonucleotides on a solid support. These libraries are useful in pharmaceutical discovery for the screening of numerous biological samples for specific interactions between the double-stranded oligonucleotides, and peptides, proteins, drugs and RNA. In a related aspect, the present invention provides libraries of conformationally restricted probes on a solid support. The probes are restricted in their movement and flexibility using double-stranded oligonucleotides as scaffolding. The probes are also useful in various screening procedures associated with drug discovery and diagnosis. The present invention further provides methods for the preparation and screening of the above libraries.
    • 用于区分完全互补的杂交体与通过一个或多个碱基对不同的那些的方法和在固体支持物上的单分子双链寡核苷酸的文库。 在一个实施方案中,本发明提供了使用核酸酶处理来提高高密度寡核苷酸阵列上杂交信号质量的方法。 在另一个实施方案中,本发明提供了使用连接反应来提高高密度寡核苷酸阵列上杂交信号质量的方法。 在另一个实施方案中,本发明提供了在固体支持物上的单分子或分子间双链寡核苷酸的文库。 这些文库在药物发现中可用于筛选许多生物样品,用于双链寡核苷酸与肽,蛋白质,药物和RNA之间的特异性相互作用。 在相关方面,本发明提供了在固体支持物上的构象限制探针的文库。 使用双链寡核苷酸作为脚手架,探针的运动和灵活性受到限制。 探针也可用于与药物发现和诊断相关的各种筛选程序。 本发明还提供了制备和筛选上述文库的方法。