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1. 发明申请

US20110245101A1 CO-LOCALIZATION AFFINITY ASSAYS 审中-公开
标题翻译：共定位亲和力测定
公开(公告)号：US20110245101A1
公开(公告)日：2011-10-06
申请号：US13079878
申请日：2011-04-05
申请人： Mark S. Chee , Igor A. Kozlov , Anita D. Wentworth , Stephanie J. Kosakovsky Pond
发明人： Mark S. Chee , Igor A. Kozlov , Anita D. Wentworth , Stephanie J. Kosakovsky Pond
IPC分类号： C40B30/04
CPC分类号： G01N33/6845 , G01N2458/10
摘要： The invention provides a new assay format for high throughput molecular binding studies at a single molecule level. The invention enables creation of binding event identifiers in a highly parallel way. Individual binding events occur between two agents of a binding pair, e.g., a protein-based binding pair or a binding pair comprising a protein and a chemical moiety. The binding event identifier created through the binding of the two binding agents is unique to that pair, and identification of the binding event identifier is indicative of the binding of these specific may be assessed through a readout that is digital in nature. The invention enables very large sets of thousands or more of different binding agents or potential binding agents to be assayed simultaneously, resolving millions or more of potential interactions, and distinguishing specific interactions from those that are less specific.
摘要翻译：本发明提供了在单一分子水平上用于高通量分子结合研究的新的测定形式。本发明能够以高度并行的方式创建绑定事件标识符。个体结合事件发生在结合对的两个试剂之间，例如基于蛋白质的结合对或包含蛋白质和化学部分的结合对。通过两个绑定代理的绑定而创建的绑定事件标识符对于该对是唯一的，并且绑定事件标识符的标识指示可以通过本质上是数字的读出来评估这些特定的绑定。本发明使得能够同时测定成千上万或更多种不同结合剂或潜在的结合剂的非常大的组，解决数百万或更多的潜在相互作用，并且将特异性相互作用与不太具体的相互作用区分开来。

2. 发明申请

US20120258871A1 PEPTIDE CONSTRUCTS AND ASSAY SYSTEMS 审中-公开
标题翻译：肽结构和测定系统
公开(公告)号：US20120258871A1
公开(公告)日：2012-10-11
申请号：US13442637
申请日：2012-04-09
申请人： Igor A. Kozlov , Mark S. Chee , Petr Capek , David A. Routenberg
发明人： Igor A. Kozlov , Mark S. Chee , Petr Capek , David A. Routenberg
IPC分类号： C40B20/04 , C40B40/06
CPC分类号： G01N33/6803 , C12N15/1062 , C12N15/1075 , C12Q2521/537
摘要： The present invention provides methods for constructing peptide construct sets and methods of use of these peptide construct sets in assay systems for peptide analysis, and in particular for use in high throughput peptide analysis. The methods allow for analysis of large sets of peptide constructs in a cost-effective manner, employing molecular biological techniques that are both robust and easily parallelized. Thus, the methods allow for the construction of peptide construct sets encompassing, e.g., the human proteome.
摘要翻译：本发明提供了用于构建肽构建体组的方法以及这些肽构建体在肽分析测定系统中的使用方法，特别是用于高通量肽分析。该方法允许以成本有效的方式分析大量肽构建体，使用分子生物学技术，其稳健且容易并行化。因此，所述方法允许构建包括例如人类蛋白质组的肽构建体组。

3. 发明申请

US20120129248A1 ASSAY TOOLS AND METHODS OF USE 审中-公开
标题翻译：测定工具和使用方法
公开(公告)号：US20120129248A1
公开(公告)日：2012-05-24
申请号：US13388229
申请日：2010-08-02
申请人： Mark S. Chee , Igor A. Kozlov
发明人： Mark S. Chee , Igor A. Kozlov
IPC分类号： G01N33/53 , C12M1/40
CPC分类号： G01N33/54366 , C12Q1/34 , C12Q1/37 , G01N33/573
摘要： The present invention provides assay tools for the detection of biological or chemical activity in a sample. The assay tools of the invention provide direct detection using a positive signal generated on a surface of the assay tool. These assay tools provide improved methods for detection and/or identification of multiple agents (e.g., enzymes) in a sample, analysis of substrate specificity of such agents, and binding affinities and specificities of such agents. Upon activity a component is released from a first immobilised construct and then captured by a capture surface. At least two different immobilised constructs are used.
摘要翻译：本发明提供了用于检测样品中生物或化学活性的测定工具。本发明的测定工具使用在测定工具的表面上产生的阳性信号提供直接检测。这些测定工具提供了用于样品中多个试剂（例如酶）的检测和/或鉴定的改进方法，这些试剂的底物特异性的分析以及这些试剂的结合亲和力和特异性。在活动时，将组分从第一固定化构建物释放，然后由捕获表面捕获。使用至少两种不同的固定化构建体。

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