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11. 发明申请

US20100015671A1 CYCLIC MALTOSYLMALTOSE, CYCLIC MALTOSYMALTOSE-FORMING ENZYME, THEIR PREPARATION AND USES 有权
标题翻译：环状麦芽糖醇，环状麦芽糖醇形成酶，其制备和用途
公开(公告)号：US20100015671A1
公开(公告)日：2010-01-21
申请号：US11941862
申请日：2007-11-16
申请人： Kazuhisa Mukai , Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Kazuhisa Mukai , Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12P19/18 , C12N9/10
CPC分类号： A61K9/2018 , A21D2/181 , A23L2/52 , A23L2/60 , A23L13/428 , A23L27/33 , A23L33/10 , A61K8/60 , A61Q11/00 , C07H3/06 , C12N9/1048 , C12P19/18
摘要： An object of the present invention is to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above object by providing a novel cyclic saccharide having a structure of cyclo{>6)-α-D-glucopyranosyl-(1>4)-α-D-glucopyranosyl-(1>6)-α-D-glucopyranosyl-(1>4)-α-D-glucopyranosyl-(1>}, cyclic maltosylmaltose, novel cyclic maltosylmaltose-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the cyclic maltosylmaltose or a saccharide composition comprising the same, and uses thereof.
摘要翻译：本发明的目的是通过提供一种由葡萄糖组成的新型非还原性糖组成并提供形成非还原性糖的新型酶，提供一种非还原性糖的方法及其制备方法，编码该酶的DNA，重组DNA和包含该DNA的转化体，包含非还原性糖的组合物及其用途。本发明通过提供具有环{> 6）-α-D-吡喃葡萄糖基 - （1,4）-alpha-D-吡喃葡萄糖基 - （1,6）-alpha-D结构的新型环状糖来解决上述目的 - 吡喃葡萄糖基 - （1,4）-alpha-D-吡喃葡萄糖基 - （1>），环状麦芽糖基麦芽糖，新的环状麦芽糖基麦芽糖酶形成酶，其制备方法和方法，编码该酶的DNA，重组DNA和转化体包含该DNA，包含环状麦芽糖麦芽糖的组合物或包含其的糖组合物及其用途。

12. 发明授权

US08633005B2 Isocyclomaltooligosaccharide(s), isocyclomaltooligosaccharide-forming enzyme, their preparation and uses 有权
标题翻译：异环寡糖，异环寡糖寡糖形成酶，其制备和应用
公开(公告)号：US08633005B2
公开(公告)日：2014-01-21
申请号：US12764927
申请日：2010-04-21
申请人： Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12N9/10 , C12N15/54
CPC分类号： C07H3/06 , A23L29/35 , A23L33/25 , C12N9/2402 , C12N9/2405 , C12P19/04
摘要： A non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents, a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof are provided by use of an isocyclomaltooligosaccharide(s) having a structure represented by Formula 1, Cyclo{→6)-[α-D-Glcp-(1→4)]n-α-D-Glcp-(1→}, wherein “n” is 4 or 5.
摘要翻译：通过提供由葡萄糖组成的新型非还原性糖类，形成非还原性糖类的新型酶，其制备方法和方法，编码该酶的DNA，重组DNA和转化体，得到非还原性糖类， DNA，包含非还原性糖的组合物及其用途通过使用具有由式1表示的结构的环状小麦寡糖，环 - { - 6） - [α-D-Glcp-（1- > 4）] n-α-D-Glcp-（1->}，其中“n”为4或5。

13. 发明授权

US07951530B2 Cyclic maltosylmaltose, cyclic maltosymaltose-forming enzyme, their preparation and uses 有权
标题翻译：循环麦芽糖苷，环状麦芽糖基酶形成酶，其制备和用途
公开(公告)号：US07951530B2
公开(公告)日：2011-05-31
申请号：US11941862
申请日：2007-11-16
申请人： Kazuhisa Mukai , Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Kazuhisa Mukai , Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： G01N33/573 , C12Q1/48 , C12P1/00 , C13K7/00 , C07H1/00
CPC分类号： A61K9/2018 , A21D2/181 , A23L2/52 , A23L2/60 , A23L13/428 , A23L27/33 , A23L33/10 , A61K8/60 , A61Q11/00 , C07H3/06 , C12N9/1048 , C12P19/18
摘要： An object of the present invention is to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above object by providing a novel cyclic saccharide having a structure of cyclo{>6)-α-D-glucopyranosyl-(1>4)-α-D-glucopyranosyl-(1>6)-α-D-glucopyranosyl-(1>4)-α-D-glucopyranosyl-(1>}, cyclic maltosylmaltose, novel cyclic maltosylmaltose-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the cyclic maltosylmaltose or a saccharide composition comprising the same, and uses thereof.
摘要翻译：本发明的目的是通过提供一种由葡萄糖组成的新型非还原性糖组成并提供形成非还原性糖的新型酶，提供一种非还原性糖的方法及其制备方法，编码该酶的DNA，重组DNA和包含该DNA的转化体，包含非还原性糖的组合物及其用途。本发明通过提供具有环{（6）-α-D-吡喃葡萄糖基 - （1,4）-α-D-吡喃葡萄糖基 - （1,6）-α-D结构的新型环状糖来解决上述目的 - 吡喃葡萄糖基 - （1> 4）-α-D-吡喃葡萄糖基 - （1>），环状麦芽糖基麦芽糖，新型环状麦芽糖基麦芽糖酶形成酶，其制备方法和方法，编码该酶的DNA，重组DNA和转化体包含该DNA，包含环状麦芽糖麦芽糖的组合物或包含其的糖组合物及其用途。

14. 发明申请

US20080241904A1 PROCESS FOR PRODUCING ISOMALTOSE AND USES THEREOF 有权
标题翻译：生产异构体的方法及其用途
公开(公告)号：US20080241904A1
公开(公告)日：2008-10-02
申请号：US11777044
申请日：2007-07-12
申请人： Michio Kubota , Tomoyuki Nishimoto , Takanobu Higashiyama , Hikaru Watanabe , Shigeharu Fukuda , Toshio Miyake
发明人： Michio Kubota , Tomoyuki Nishimoto , Takanobu Higashiyama , Hikaru Watanabe , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12N9/10
CPC分类号： C12P19/12
摘要： A novel process for producing isomaltose and uses thereof and comprising the steps of contacting saccharides, which have a glucose polymerization degree of at least two and α-1,4 glucosidic linkage as a linkage at the non-reducing end, with an α-isomaltosylglucosaccharide-forming enzyme, in the presence or the absence of α-isomaltosyl-transferring enzyme to form a-isomaltosylglucosaccharides, which have a glucose polymerization degree of at least three, α-1,6 glucosidic linkage as a linkage at the non-reducing end, and α-1,4 glucosidic linkage as a linkage other than the non-reducing end, and/or to form cyclo{→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→6)-α-D-glucopyranosyl-(1″3)-α-D-glucopyranosyl-(1→}; contacting the saccharides so formed with isomaltose-releasing enzyme to release isomaltose; and collecting the released isomaltose; and uses thereof.
摘要翻译：一种制备异麦芽糖的新方法及其用途，包括以下步骤：将具有至少两个葡萄糖聚合度的至少两个和非1,4-还原末端的α-1,4糖苷键的糖与α-异麦芽糖基葡萄糖在α-异麦芽糖基转移酶的存在或不存在下形成α-异麦芽糖基糖，其具有在非还原末端具有至少三个α-1,6糖苷键作为键的葡萄糖聚合度和α-1,4糖苷键作为非还原末端之外的键，和/或形成环{ - > 6）-al-D-glucopyranosyl-（1-> 3）-al-D-glucopyranosyl - （1-> 6）-al-D-glucopyranosyl-（1''3）-alpha-D-吡喃葡萄糖基 - （1->};使所形成的糖与异麦芽糖释放酶接触以释放异麦芽糖; 释放的异麦芽糖;及其用途。

15. 发明申请

US20080131468A1 Isocyclomaltooligosaccharide (S) , Isocyclomaltooligosaccharide-Forming Enzyme, Their Preparation and Uses 有权
标题翻译：异环寡糖（S），异环寡糖 - 形成酶，其制备及用途
公开(公告)号：US20080131468A1
公开(公告)日：2008-06-05
申请号：US11663919
申请日：2005-09-26
申请人： Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： A61K8/02 , C13K5/00 , C12N9/00 , C12N1/20 , A61K31/715 , A23L2/38 , A23G3/00 , C07H21/04 , C12N1/00 , C12P19/12
CPC分类号： C07H3/06 , A23L29/35 , A23L33/25 , C12N9/2402 , C12N9/2405 , C12P19/04
摘要： The present invention have objects to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above objects by providing an isocyclomaltooligosaccharide(s) having a structure represented by General Formula 1, a novel isocyclomaltooligosaccharide-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the isocyclomaltooligosaccharide (s) or a saccharide composition comprising the same, and uses thereof. Cyclo{→6)-[α-D-Glcp-(1→4)]n-α-D-Glcp-(1→}General Formula 1 (In General Formula 1, “n” means a number of 4 or 5)
摘要翻译：本发明的目的是提供一种由葡萄糖组成的新型非还原性糖组成的非还原性糖类，提供形成非还原性糖类的新型酶，其制造方法及其制造方法，编码酶的DNA，重组DNA和包含DNA的转化体，包含非还原性糖的组合物及其用途。本发明通过提供具有由通式1表示的结构的异环型低聚半乳糖，新型的异环型低聚葡萄糖形成酶，其制备方法和方法，编码该酶的DNA，重组DNA和转化体来解决上述目的包含DNA，包含异环寡脱醇糖的组合物或包含该组合物的糖组合物及其用途。 <？in-line-formula description =“In-line Formulas”end =“lead”？> Cyclo { - > 6） - [alpha-D-Glcp-（1→4）] }通式1 <？in-line-formula description =“In-line Formulas”end =“tail”？>（在通用公式1中，“n” 4或5）

16. 发明申请

US20070066565A1 Cyclic maltosylmatose 有权
公开(公告)号：US20070066565A1
公开(公告)日：2007-03-22
申请号：US10569959
申请日：2004-08-26
申请人： Kazuhisa Mukai , Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Kazuhisa Mukai , Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12P19/04 , C07H21/04 , A61K31/715 , C12P21/06 , C12N9/10
CPC分类号： A61K9/2018 , A21D2/181 , A23L2/52 , A23L2/60 , A23L13/428 , A23L27/33 , A23L33/10 , A61K8/60 , A61Q11/00 , C07H3/06 , C12N9/1048 , C12P19/18
摘要： An object of the present invention is to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above object by providing a novel cyclic saccharide having a structure of cyclo{→6)-α-D-glucopyranosyl-(1→4)-α-D-glucopyranosyl-(1→6)-α-D-glucopyranosyl-(1→4)-α-D-glucopyranosyl-(1→6}, cyclic maltosylmaltose, novel cyclic maltosylmaltose-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the cyclic maltosylmaltose or a saccharide composition comprising the same, and uses thereof.

17. 发明授权

US07186824B2 Reduction inhibitory agent for active-oxygen eliminating activity 有权
标题翻译：活性氧消除活性的还原抑制剂
公开(公告)号：US07186824B2
公开(公告)日：2007-03-06
申请号：US10670525
申请日：2003-09-26
申请人： Hajime Aga , Takashi Shibuya , Shigeharu Fukuda , Toshio Miyake
发明人： Hajime Aga , Takashi Shibuya , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C07H1/00
CPC分类号： A61K31/7016 , A61K36/03 , A61K36/07 , A61K36/11 , A61K36/15 , A61K36/16 , A61K36/185 , A61K36/21 , A61K36/23 , A61K36/232 , A61K36/27 , A61K36/28 , A61K36/282 , A61K36/287 , A61K36/31 , A61K36/315 , A61K36/42 , A61K36/45 , A61K36/46 , A61K36/48 , A61K36/482 , A61K36/484 , A61K36/515 , A61K36/52 , A61K36/53 , A61K36/534 , A61K36/535 , A61K36/67 , A61K36/68 , A61K36/70 , A61K36/73 , A61K36/734 , A61K36/736 , A61K36/75 , A61K36/752 , A61K36/77 , A61K36/81 , A61K36/82 , A61K36/87 , A61K36/88 , A61K36/886 , A61K36/888 , A61K36/8945 , A61K36/8962 , A61K36/899 , A61K36/8994 , A61K36/8998 , A61K36/9066 , A61K36/9068 , A61K2300/00
摘要： A reduction inhibitory agent for active-oxygen eliminating activity comprises trehalose as an effective ingredient. A method for inhibiting the reduction of active-oxygen eliminating activity comprises incorporating either trehalose or the reduction inhibitory agent into plant edible products and/or plant antioxidants. A composition is provided that reduces the active-oxygen eliminating activity of plant edible products and/or plant antioxidants.
摘要翻译：活性氧消除活性的还原抑制剂包括海藻糖作为有效成分。抑制活性氧消除活性降低的方法包括将海藻糖或还原抑制剂掺入植物性食品和/或植物抗氧化剂中。提供了降低植物可食用产品和/或植物抗氧化剂的活性氧消除活性的组合物。

18. 发明授权

US5863771A Saccharide composition comprising maltooligosylturanose and maltooligosylpalatinose, its preparation and uses 失效
标题翻译：包含麦芽低聚糖尿糖和麦芽低聚糖尿苷的糖组合物，其制备和用途
公开(公告)号：US5863771A
公开(公告)日：1999-01-26
申请号：US810363
申请日：1997-03-03
申请人： Hajime Aga , Takashi Shibuya , Shigeharu Fukuda , Toshio Miyake
发明人： Hajime Aga , Takashi Shibuya , Shigeharu Fukuda , Toshio Miyake
IPC分类号： A23L27/00 , A23L27/30 , A61K8/60 , A61K8/73 , A61K31/70 , A61K31/702 , A61P17/00 , A61Q11/00 , A61Q19/00 , C07H3/06 , C08B37/00 , C12P19/04 , C12P19/18 , C13K13/00 , A23G3/00 , A61K31/715 , C08B3/06
CPC分类号： A61Q11/00 , A23L27/33 , A61K8/73 , A61Q19/00 , C07H3/06 , C12P19/04 , C12P19/18 , C13K13/00
摘要： A saccharide composition comprising maltooligosyl derivatives of turanose and palatinose which can be readily produced, separated, and purified in a relatively-high yield from maltooligosylsucrose by allowing non-reducing saccharide-forming enzymes to act on aqueous solutions containing maltooligosylsucrose. These saccharides are reducing oligosaccharides with a mild and high-quality sweetness and can be used orally and parenterally, as well as being readily metabolized and used by living bodies.
摘要翻译：一种糖组合物，其包含吐露糖和帕拉金糖的麦芽寡糖基衍生物，其可以通过使非还原性糖形成酶作用于含有麦芽寡糖糖基蔗糖的水溶液而容易地从麦芽寡糖蔗糖以相对高的产率产生，分离和纯化。这些糖类是降低具有轻度和高质量甜度的寡糖，可以口服和肠胃外使用，并且易于被活体代谢和使用。

19. 发明申请

US20080027027A1 Lactosucrose High Content Saccharide, Its Preparation and Uses 审中-公开
标题翻译：乳糖蔗糖高含量糖，其制备和用途
公开(公告)号：US20080027027A1
公开(公告)日：2008-01-31
申请号：US11587248
申请日：2005-04-20
申请人： Hiroyuki Okabe , Hajime Aga , Michio Kubota , Toshio Miyake
发明人： Hiroyuki Okabe , Hajime Aga , Michio Kubota , Toshio Miyake
IPC分类号： A61K31/702 , A23L1/236 , A61K8/72 , A61P1/00 , A61Q99/00 , C12P19/00 , C13D3/16 , C13F1/02 , C13F3/00
CPC分类号： C13B30/02 , A23L27/33 , C07H3/06 , C12P19/14 , C12P19/18 , C13B20/142
摘要： An object of the present invention is to provide a lactosucrose high content saccharide which comprises 70% or more of lactosucrose, on a saccharide composition basis, with a lower content of 1-kestose and fructosyl lactosucrose as by-products. Another object of the present invention is to provide processes for producing a lactosucrose high content saccharide and high purity lactosucrose containing 90% or more of lactosucrose, on a saccharide composition basis, which are feasible for industrial production. Further object of the present invention is to provide a solid lactosucrose with low hygroscopicity and a solid composition comprising the same. The present invention solves above objects by providing a lactosucrose high content saccharide comprising 70% or more of lactosucrose and less than 3% of the total amount of 1-kestose and fructosyl lactosucrose, on a saccharide composition basis; a process for producing the lactosucrose high content saccharide, comprising the steps of allowing β-fructofuranosidase, derived from a microorganism belonging to the genus Bacillus, and sucrose-unassimilable yeast to contact with an aqueous solution containing sucrose and lactose to obtain a reaction mixture comprising 70% or more of lactosucrose and less than 3% of the total amount of 1-kestose and fructosyl lactosucrose and collecting the resulting lactosucrose high content saccharide; a process for producing a high purity lactosucrose, comprising the steps of subjecting the lactosucrose high content saccharide to a chromatography using a resin and collecting fractions containing 90% or more of lactosucrose, on a saccharide composition basis; a process for producing crystalline lactosucrose; and various solid compositions prepared by incorporating the crystalline lactosucrose.
摘要翻译：本发明的目的是提供一种乳糖蔗糖高含量糖，其以糖组成为基础含有70％以上的乳糖蔗糖，其中1-kestose和果糖基乳糖为副产物含量较低。本发明的另一个目的是提供一种以糖组成为基础制备含有90％以上乳蔗糖的乳糖蔗糖高含量糖和高纯度乳糖蔗糖的方法，其可用于工业生产。本发明的另一个目的是提供具有低吸湿性的固体乳糖蔗糖和包含它的固体组合物。本发明通过以糖组成为基础，提供含有70％以上的乳糖和低于1-kestose和果糖乳糖蔗糖总量的3％的乳糖蔗糖高含量糖，一种生产乳糖蔗糖高含量糖的方法，包括以下步骤：将衍生自属于芽孢杆菌属的微生物的β-呋喃果糖苷酶和蔗糖不可同化的酵母与含有蔗糖和乳糖的水溶液接触，得到包含 70％以上的乳蔗糖，少于1-kestose和果糖乳糖蔗糖总量的3％，并收集得到的乳糖蔗糖高含量糖; 一种生产高纯度乳糖蔗糖的方法，包括以糖组成为基础，使用树脂对乳糖蔗糖高含量糖进行色谱分离并收集含有90％以上乳蔗糖的级分的步骤; 一种生产结晶乳糖的方法; 和通过掺入结晶乳蔗糖制备的各种固体组合物。

20. 发明授权

US08524889B2 Highly-branched starch, its production and uses 有权
标题翻译：高分支淀粉，其生产和用途
公开(公告)号：US08524889B2
公开(公告)日：2013-09-03
申请号：US13443296
申请日：2012-04-10
申请人： Tomoyuki Nishimoto , Katsuhiko Hino , Takanori Okura , Hiroto Chaen , Shigeharu Fukuda , Toshio Miyake
发明人： Tomoyuki Nishimoto , Katsuhiko Hino , Takanori Okura , Hiroto Chaen , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C07H1/06 , C08B31/00
CPC分类号： C12P19/18 , A23L33/10 , A23V2002/00 , A61K9/0014 , A61K9/0056 , A61K9/06 , A61K9/2054 , A61K9/4816 , A61K9/7007 , A61K31/718 , C08B30/12 , A23V2200/302 , A23V2250/5118
摘要： An object of the present invention is to provide a novel starchy substance having a retrogradation-resistance, a process for producing the starchy substance efficiently from a material starch by enzymatic reaction, and uses thereof. The present invention attains the above object by providing branched starch having 6-α-maltosyl- and/or 6-α-maltotetraosyl-structure(s) with a marked retrogradation-resistance, a process for producing the branched starch without lowering the molecular weight of material starch, and uses thereof.
摘要翻译：本发明的目的是提供一种具有抗退行性的新型淀粉物质，通过酶反应从材料淀粉有效地生产淀粉质物质的方法及其应用。本发明通过提供具有显着抗退行性的具有6-α-麦芽糖基 - 和/或6-α-麦芽四糖基结构的支链淀粉来实现上述目的，一种生产支化淀粉而不降低分子量的方法的材料淀粉及其用途。

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