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1. 发明授权

US10443051B2 Direct cloning 有权
公开(公告)号：US10443051B2
公开(公告)日：2019-10-15
申请号：US13806086
申请日：2011-06-10
申请人： Youming Zhang , Jun Fu , Adrian Francis Stewart
发明人： Youming Zhang , Jun Fu , Adrian Francis Stewart
IPC分类号： C12N15/10 , C12N9/22 , C12N15/90
摘要： A method for performing homologous recombination between at least a first nucleic acid molecule and a second nucleic acid molecule which share at least one region of sequence homology. A method for improving the efficiency of homologous recombination.

2. 发明授权

US08728820B2 Method of nucleic acid recombination 有权
标题翻译：核酸重组方法
公开(公告)号：US08728820B2
公开(公告)日：2014-05-20
申请号：US12918134
申请日：2009-02-20
申请人： Marcello Maresca , Axel Steffen Erler , Jun Fu , Philipp Martin Seibert , Adrian Francis Stewart , Youming Zhang
发明人： Marcello Maresca , Axel Steffen Erler , Jun Fu , Philipp Martin Seibert , Adrian Francis Stewart , Youming Zhang
IPC分类号： C12N15/90 , C12N15/09
CPC分类号： C12N15/1082
摘要： The invention provides a method for inserting a single stranded replacement nucleic acid into a target nucleic acid, the method comprising the steps of: a) generating a single stranded replacement nucleic acid from a double stranded nucleic acid, wherein the double stranded nucleic acid is adapted at one or both of its 5′ ends such that preferential degradation of one strand and/or strand separation generates the single stranded replacement nucleic acid, wherein the single stranded replacement nucleic acid comprises a 5′ region that is identical to sequence on the target nucleic acid, a 3′ region that is identical to sequence on the target nucleic acid and optionally a replacement region between the 5′ and 3′ regions that is not identical to sequence on the target nucleic acid, b) exposing the target nucleic acid to the single stranded replacement nucleic acid under conditions suitable for recombination to occur between the single stranded replacement nucleic acid and the target nucleic acid, and c) selecting a target nucleic acid whose sequence has been altered by inclusion of said single stranded replacement nucleic acid. Other methods for modifying target nucleic acids are also provided.
摘要翻译：本发明提供了将单链置换核酸插入靶核酸的方法，所述方法包括以下步骤：a）从双链核酸产生单链置换核酸，其中双链核酸适应在其5'末端的一个或两个上，使得一条链和/或链分离的优先降解产生单链置换核酸，其中单链置换核酸包含与靶核酸上的序列相同的5'区酸，与靶核酸上的序列相同的3'区，以及任选地在与靶核酸上的序列不相同的5'和3'区之间的置换区，b）将靶核酸暴露于在单链置换核酸和焦油之间发生适合重组的条件下，单链置换核酸得到核酸，和c）选择其核苷酸序列已经通过包含所述单链置换核酸而被改变。还提供了用于修饰靶核酸的其它方法。

3. 发明授权

US09580717B2 Heterologous hosts 有权
标题翻译：异种宿主
公开(公告)号：US09580717B2
公开(公告)日：2017-02-28
申请号：US13516758
申请日：2010-12-17
申请人： Youming Zhang , Jun Fu , Xiaoying Bian , Adrian Francis Stewart , Rolf Müller
发明人： Youming Zhang , Jun Fu , Xiaoying Bian , Adrian Francis Stewart , Rolf Müller
IPC分类号： C12N15/74 , C07D245/02 , C07D417/06 , C07D493/04 , C12N9/90 , C12N15/52 , C12P17/10 , C12P17/18 , C12N15/70 , A61K35/00 , C12N9/00
CPC分类号： C12N15/70 , A61K35/74 , A61K2035/11 , C07D245/02 , C07D417/06 , C07D493/04 , C07D498/22 , C07G17/00 , C07K5/06139 , C07K7/64 , C12N9/1288 , C12N9/90 , C12N9/93 , C12N15/52 , C12N15/74 , C12P17/10 , C12P17/181 , C12Y207/08007 , Y02P20/52
摘要： This invention is related to bacterial engineering and the heterologous expression of useful compounds. In particular, the invention relates to a heterologous host that has been engineered for expression of a gene which is capable of polyketide or non-ribosomal peptide synthesis. Methods of treating cancer are also disclosed.
摘要翻译：本发明涉及细菌工程和有用化合物的异源表达。特别地，本发明涉及已被改造用于表达能够进行聚酮化合物或非核糖体肽合成的基因的异源宿主。还公开了治疗癌症的方法。

4. 发明申请

US20130210681A1 Direct Cloning 审中-公开
标题翻译：直接克隆
公开(公告)号：US20130210681A1
公开(公告)日：2013-08-15
申请号：US13806086
申请日：2011-06-10
申请人： Youming Zhang , Jun Fu , Adrian Francis Stewart
发明人： Youming Zhang , Jun Fu , Adrian Francis Stewart
IPC分类号： C12N15/10
摘要： A method for performing homologous recombination between at least a first nucleic acid molecule and a second nucleic acid molecule which share at least one region of sequence homology. A method for improving the efficiency of homologous recombination.
摘要翻译：在至少第一核酸分子与共有至少一个序列同源性区域的第二核酸分子之间进行同源重组的方法。一种提高同源重组效率的方法。

5. 发明申请

US20110165630A1 Method of Nucleic Acid Recombination 有权
标题翻译：核酸重组方法
公开(公告)号：US20110165630A1
公开(公告)日：2011-07-07
申请号：US12918134
申请日：2009-02-20
申请人： Marcello Maresca , Axel Steffen Erler , Jun Fu , Philipp Martin Seibert , Adrian Francis Stewart , Youming Ahang
发明人： Marcello Maresca , Axel Steffen Erler , Jun Fu , Philipp Martin Seibert , Adrian Francis Stewart , Youming Ahang
IPC分类号： C12P19/34 , C07H1/00 , C12N1/21 , C12N15/70 , C07K2/00 , C12N15/87
CPC分类号： C12N15/1082
摘要： The invention provides a method for inserting a single stranded replacement nucleic acid into a target nucleic acid, said method comprising the steps of: a) generating a single stranded replacement nucleic acid from a double stranded nucleic acid, wherein the double stranded nucleic acid is adapted at one or both of its 5′ ends such that preferential degradation of one strand and/or strand separation generates the single stranded replacement nucleic acid, wherein the single stranded replacement nucleic acid comprises a 5′ region that is identical to sequence on the target nucleic acid, a 3′ region that is identical to sequence on the target nucleic acid and optionally a replacement region between the 5′ and 3′ regions that is not identical to sequence on the target N nucleic acid, b) exposing the target nucleic acid to the single stranded replacement nucleic acid under conditions suitable for recombination to occur between the single stranded replacement nucleic acid and the target nucleic acid, and c) selecting a target nucleic acid whose sequence has been altered by inclusion of said single stranded replacement nucleic acid. Other methods for modifying target nucleic acids are also provided.
摘要翻译：本发明提供了将单链置换核酸插入靶核酸的方法，所述方法包括以下步骤：a）从双链核酸产生单链置换核酸，其中双链核酸适应在其5'末端的一个或两个上，使得一条链和/或链分离的优先降解产生单链置换核酸，其中单链置换核酸包含与靶核酸上的序列相同的5'区酸，与靶核酸上的序列相同的3'区域，以及任选地在与目标N核酸上的序列不相同的5'和3'区域之间的置换区域，b）将靶核酸暴露于单链置换核酸在适于重组的条件下发生在单链置换核酸和靶核酸，以及c）通过包含所述单链置换核酸来选择其序列已被改变的靶核酸。还提供了用于修饰靶核酸的其它方法。

6. 发明申请

US20130089522A1 HETEROLOGOUS HOSTS 有权
标题翻译：异物宿主
公开(公告)号：US20130089522A1
公开(公告)日：2013-04-11
申请号：US13516758
申请日：2010-12-17
申请人： Youming Zhang , Jun Fu , Xiaoying Bian , Francis Stewart , Rolf Muller
发明人： Youming Zhang , Jun Fu , Xiaoying Bian , Francis Stewart , Rolf Muller
IPC分类号： C12N15/74 , C12N15/70
CPC分类号： C12N15/70 , A61K35/74 , A61K2035/11 , C07D245/02 , C07D417/06 , C07D493/04 , C07D498/22 , C07G17/00 , C07K5/06139 , C07K7/64 , C12N9/1288 , C12N9/90 , C12N9/93 , C12N15/52 , C12N15/74 , C12P17/10 , C12P17/181 , C12Y207/08007 , Y02P20/52
摘要： This invention is related to bacterial engineering and the heterologous expression of useful compounds. In particular, the invention relates to a heterologous host that has been engineered for expression of a gene which is capable of polyketide or non-ribosomal peptide synthesis. Methods of treating cancer are also disclosed.
摘要翻译：本发明涉及细菌工程和有用化合物的异源表达。特别地，本发明涉及已被改造用于表达能够进行聚酮化合物或非核糖体肽合成的基因的异源宿主。还公开了治疗癌症的方法。

7. 发明申请

US20090298117A1 METHODS FOR HETEROLOGOUS EXPRESSION OF SECONDARY METABOLITES 审中-公开
标题翻译：二次代谢物的异位表达方法
公开(公告)号：US20090298117A1
公开(公告)日：2009-12-03
申请号：US11577972
申请日：2005-10-26
申请人： Youming Zhang , Rolf Müller , Francis Stewart , Frank Gross , Silke C. Wenzel , Jun Fu
发明人： Youming Zhang , Rolf Müller , Francis Stewart , Frank Gross , Silke C. Wenzel , Jun Fu
IPC分类号： C12P39/00
CPC分类号： C12P17/14
摘要： The invention provides a method for the heterologous expression of a secondary metabolite encoded by a biosynthetic pathway. Also provided is a method for introducing a large sized DNA molecule into the chromosome of a heterologous host using a transposable element. Novel myxochromide S derivatives are also provided.
摘要翻译：本发明提供了由生物合成途径编码的次级代谢物异源表达的方法。还提供了使用转座元件将大尺寸DNA分子引入异源宿主染色体的方法。还提供了新的色氨酸S衍生物。

8. 发明授权

US09266062B2 Method of removing sulfur oxides and nitrogen oxides in the flue gas 有权
标题翻译：去除烟气中硫氧化物和氮氧化物的方法
公开(公告)号：US09266062B2
公开(公告)日：2016-02-23
申请号：US14235518
申请日：2012-07-27
申请人： Dawei Guo , Jiushun Zhang , Anguo Mao , Chaogang Xie , Jun Fu , Wei Wang , Jingchuan Yu
发明人： Dawei Guo , Jiushun Zhang , Anguo Mao , Chaogang Xie , Jun Fu , Wei Wang , Jingchuan Yu
IPC分类号： B01D53/86 , B01D53/50 , B01D53/56 , B01J23/10 , B01J23/63 , B01J23/83 , C01B17/04 , C10G11/18
CPC分类号： B01D53/8637 , B01D2251/20 , B01D2251/202 , B01D2251/204 , B01D2251/208 , B01D2255/10 , B01D2255/1021 , B01D2255/206 , B01D2255/2063 , B01D2255/2065 , B01D2255/207 , B01D2255/20715 , B01D2255/20746 , B01D2255/20769 , B01D2255/2092 , B01D2255/9022 , B01D2255/904 , B01J23/10 , B01J23/63 , B01J23/83 , C01B17/0404 , C01B17/0434 , C01B17/0473 , C10G11/182 , Y02P20/129
摘要： The present invention discloses a method of removing sulfur oxides and/or nitrogen oxides in a regeneration flue gas emitted from a regenerator of a catalytic cracking plant and recovering the elemental sulfur and an apparatus therefor.
摘要翻译：本发明公开了一种从催化裂化装置的再生器排出的再生烟气中除去硫氧化物和/或氮氧化物并回收元素硫的方法及其装置。

9. 发明授权

US09151725B2 Humidity measurement device and method 有权
标题翻译：湿度测量装置及方法
公开(公告)号：US09151725B2
公开(公告)日：2015-10-06
申请号：US13518028
申请日：2010-04-26
申请人： Jun Fu , Wei Chang , Lianbo Yu
发明人： Jun Fu , Wei Chang , Lianbo Yu
IPC分类号： G01N27/22
CPC分类号： G01N27/223 , G01N27/228
摘要： A humidity measurement device comprises a capacitance measurement unit (101) and a microcontroller (102). The capacitance measurement unit (101) is used to output a frequency signal of a humidity-sensitive capacitor (C1) by the humidity-sensitive capacitor (C1) and a frequency signal of a reference capacitor (C2) by the reference capacitor (C2) respectively. The microcontroller (102) is used to obtain a frequency value of the humidity-sensitive capacitor (C1) and a frequency value of the reference capacitor (C2) based on the frequency signal of the humidity-sensitive capacitor (C1) and the frequency signal of the reference capacitor (C2), and obtain a current ambient humidity value by using a linear function relationship of a frequency difference between the humidity-sensitive capacitor (C1) and the reference capacitor (C2) and a humidity value. A humidity measurement method includes using the said humidity measurement device to measure a current ambient humidity value.
摘要翻译：湿度测量装置包括电容测量单元（101）和微控制器（102）。电容测量单元（101）用于通过湿度敏感电容器（C1）输出湿度敏感电容器（C1）的频率信号和参考电容器（C2）的参考电容器（C2）的频率信号，分别。微控制器（102）用于根据湿度敏感电容器（C1）的频率信号和频率信号来获得湿度敏感电容器（C1）的频率值和参考电容器（C2）的频率值的参考电容器（C2）的频率差的线性函数关系，并且通过使用湿度敏感电容器（C1）和参考电容器（C2）之间的频率差的线性函数关系和湿度值来获得当前的环境湿度值。湿度测量方法包括使用所述湿度测量装置来测量当前的环境湿度值。

10. 发明授权

US09316437B2 Microwave vacuum-drying of organic materials 有权
标题翻译：微波真空干燥有机材料
公开(公告)号：US09316437B2
公开(公告)日：2016-04-19
申请号：US13521410
申请日：2010-10-25
申请人： Jun Fu , Timothy D. Durance , Parastoo Yaghmaee
发明人： Jun Fu , Timothy D. Durance , Parastoo Yaghmaee
IPC分类号： F26B3/347 , F26B5/04 , F26B17/04 , A23L3/01 , A23L3/54 , A23B4/03 , A23B7/02 , A23N12/08
CPC分类号： F26B5/048 , A23B4/031 , A23B7/02 , A23L3/01 , A23L3/54 , A23L5/34 , A23N12/08 , A23V2002/00 , F26B3/347 , F26B5/041 , F26B17/04
摘要： An apparatus 20 for microwave vacuum-drying organic materials such as foods and bioactives has a plurality of microwave generators 50 actuated so as to cause interference between their respective microwave streams and evenly distribute the microwave energy across the vacuum chamber 34. The microwave-transparent window 36 in the chamber is arranged so the organic material to be dried is moved across it on a conveyor belt 60 and the microwave energy passing into the chamber 34 immediately encounters the organic materials, thus attenuating the energy and reducing arcing.
摘要翻译：用于微波真空干燥有机材料如食品和生物活性物质的装置20具有致动多个微波发生器50，以便引起它们各自的微波流之间的干扰，并将微波能均匀分布在真空室34内。微波透明窗 36被布置成使得待干燥的有机材料在传送带60上移动穿过它，并且进入室34的微波能量立即遇到有机材料，从而衰减能量并减少电弧。

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