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    • 2. 发明授权
    • Method of nucleic acid recombination
    • 核酸重组方法
    • US08728820B2
    • 2014-05-20
    • US12918134
    • 2009-02-20
    • Marcello MarescaAxel Steffen ErlerJun FuPhilipp Martin SeibertAdrian Francis StewartYouming Zhang
    • Marcello MarescaAxel Steffen ErlerJun FuPhilipp Martin SeibertAdrian Francis StewartYouming Zhang
    • C12N15/90C12N15/09
    • C12N15/1082
    • The invention provides a method for inserting a single stranded replacement nucleic acid into a target nucleic acid, the method comprising the steps of: a) generating a single stranded replacement nucleic acid from a double stranded nucleic acid, wherein the double stranded nucleic acid is adapted at one or both of its 5′ ends such that preferential degradation of one strand and/or strand separation generates the single stranded replacement nucleic acid, wherein the single stranded replacement nucleic acid comprises a 5′ region that is identical to sequence on the target nucleic acid, a 3′ region that is identical to sequence on the target nucleic acid and optionally a replacement region between the 5′ and 3′ regions that is not identical to sequence on the target nucleic acid, b) exposing the target nucleic acid to the single stranded replacement nucleic acid under conditions suitable for recombination to occur between the single stranded replacement nucleic acid and the target nucleic acid, and c) selecting a target nucleic acid whose sequence has been altered by inclusion of said single stranded replacement nucleic acid. Other methods for modifying target nucleic acids are also provided.
    • 本发明提供了将单链置换核酸插入靶核酸的方法,所述方法包括以下步骤:a)从双链核酸产生单链置换核酸,其中双链核酸适应 在其5'末端的一个或两个上,使得一条链和/或链分离的优先降解产生单链置换核酸,其中单链置换核酸包含与靶核酸上的序列相同的5'区 酸,与靶核酸上的序列相同的3'区,以及任选地在与靶核酸上的序列不相同的5'和3'区之间的置换区,b)将靶核酸暴露于 在单链置换核酸和焦油之间发生适合重组的条件下,单链置换核酸 得到核酸,和c)选择其核苷酸序列已经通过包含所述单链置换核酸而被改变。 还提供了用于修饰靶核酸的其它方法。
    • 5. 发明申请
    • Method of Nucleic Acid Recombination
    • 核酸重组方法
    • US20110165630A1
    • 2011-07-07
    • US12918134
    • 2009-02-20
    • Marcello MarescaAxel Steffen ErlerJun FuPhilipp Martin SeibertAdrian Francis StewartYouming Ahang
    • Marcello MarescaAxel Steffen ErlerJun FuPhilipp Martin SeibertAdrian Francis StewartYouming Ahang
    • C12P19/34C07H1/00C12N1/21C12N15/70C07K2/00C12N15/87
    • C12N15/1082
    • The invention provides a method for inserting a single stranded replacement nucleic acid into a target nucleic acid, said method comprising the steps of: a) generating a single stranded replacement nucleic acid from a double stranded nucleic acid, wherein the double stranded nucleic acid is adapted at one or both of its 5′ ends such that preferential degradation of one strand and/or strand separation generates the single stranded replacement nucleic acid, wherein the single stranded replacement nucleic acid comprises a 5′ region that is identical to sequence on the target nucleic acid, a 3′ region that is identical to sequence on the target nucleic acid and optionally a replacement region between the 5′ and 3′ regions that is not identical to sequence on the target N nucleic acid, b) exposing the target nucleic acid to the single stranded replacement nucleic acid under conditions suitable for recombination to occur between the single stranded replacement nucleic acid and the target nucleic acid, and c) selecting a target nucleic acid whose sequence has been altered by inclusion of said single stranded replacement nucleic acid. Other methods for modifying target nucleic acids are also provided.
    • 本发明提供了将单链置换核酸插入靶核酸的方法,所述方法包括以下步骤:a)从双链核酸产生单链置换核酸,其中双链核酸适应 在其5'末端的一个或两个上,使得一条链和/或链分离的优先降解产生单链置换核酸,其中单链置换核酸包含与靶核酸上的序列相同的5'区 酸,与靶核酸上的序列相同的3'区域,以及任选地在与目标N核酸上的序列不相同的5'和3'区域之间的置换区域,b)将靶核酸暴露于 单链置换核酸在适于重组的条件下发生在单链置换核酸和 靶核酸,以及c)通过包含所述单链置换核酸来选择其序列已被改变的靶核酸。 还提供了用于修饰靶核酸的其它方法。
    • 9. 发明授权
    • Humidity measurement device and method
    • 湿度测量装置及方法
    • US09151725B2
    • 2015-10-06
    • US13518028
    • 2010-04-26
    • Jun FuWei ChangLianbo Yu
    • Jun FuWei ChangLianbo Yu
    • G01N27/22
    • G01N27/223G01N27/228
    • A humidity measurement device comprises a capacitance measurement unit (101) and a microcontroller (102). The capacitance measurement unit (101) is used to output a frequency signal of a humidity-sensitive capacitor (C1) by the humidity-sensitive capacitor (C1) and a frequency signal of a reference capacitor (C2) by the reference capacitor (C2) respectively. The microcontroller (102) is used to obtain a frequency value of the humidity-sensitive capacitor (C1) and a frequency value of the reference capacitor (C2) based on the frequency signal of the humidity-sensitive capacitor (C1) and the frequency signal of the reference capacitor (C2), and obtain a current ambient humidity value by using a linear function relationship of a frequency difference between the humidity-sensitive capacitor (C1) and the reference capacitor (C2) and a humidity value. A humidity measurement method includes using the said humidity measurement device to measure a current ambient humidity value.
    • 湿度测量装置包括电容测量单元(101)和微控制器(102)。 电容测量单元(101)用于通过湿度敏感电容器(C1)输出湿度敏感电容器(C1)的频率信号和参考电容器(C2)的参考电容器(C2)的频率信号, 分别。 微控制器(102)用于根据湿度敏感电容器(C1)的频率信号和频率信号来获得湿度敏感电容器(C1)的频率值和参考电容器(C2)的频率值 的参考电容器(C2)的频率差的线性函数关系,并且通过使用湿度敏感电容器(C1)和参考电容器(C2)之间的频率差的线性函数关系和湿度值来获得当前的环境湿度值。 湿度测量方法包括使用所述湿度测量装置来测量当前的环境湿度值。