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1. 发明申请

US20130089522A1 HETEROLOGOUS HOSTS 有权
标题翻译：异物宿主
公开(公告)号：US20130089522A1
公开(公告)日：2013-04-11
申请号：US13516758
申请日：2010-12-17
申请人： Youming Zhang , Jun Fu , Xiaoying Bian , Francis Stewart , Rolf Muller
发明人： Youming Zhang , Jun Fu , Xiaoying Bian , Francis Stewart , Rolf Muller
IPC分类号： C12N15/74 , C12N15/70
CPC分类号： C12N15/70 , A61K35/74 , A61K2035/11 , C07D245/02 , C07D417/06 , C07D493/04 , C07D498/22 , C07G17/00 , C07K5/06139 , C07K7/64 , C12N9/1288 , C12N9/90 , C12N9/93 , C12N15/52 , C12N15/74 , C12P17/10 , C12P17/181 , C12Y207/08007 , Y02P20/52
摘要： This invention is related to bacterial engineering and the heterologous expression of useful compounds. In particular, the invention relates to a heterologous host that has been engineered for expression of a gene which is capable of polyketide or non-ribosomal peptide synthesis. Methods of treating cancer are also disclosed.
摘要翻译：本发明涉及细菌工程和有用化合物的异源表达。特别地，本发明涉及已被改造用于表达能够进行聚酮化合物或非核糖体肽合成的基因的异源宿主。还公开了治疗癌症的方法。

2. 发明申请

US20090298117A1 METHODS FOR HETEROLOGOUS EXPRESSION OF SECONDARY METABOLITES 审中-公开
标题翻译：二次代谢物的异位表达方法
公开(公告)号：US20090298117A1
公开(公告)日：2009-12-03
申请号：US11577972
申请日：2005-10-26
申请人： Youming Zhang , Rolf Müller , Francis Stewart , Frank Gross , Silke C. Wenzel , Jun Fu
发明人： Youming Zhang , Rolf Müller , Francis Stewart , Frank Gross , Silke C. Wenzel , Jun Fu
IPC分类号： C12P39/00
CPC分类号： C12P17/14
摘要： The invention provides a method for the heterologous expression of a secondary metabolite encoded by a biosynthetic pathway. Also provided is a method for introducing a large sized DNA molecule into the chromosome of a heterologous host using a transposable element. Novel myxochromide S derivatives are also provided.
摘要翻译：本发明提供了由生物合成途径编码的次级代谢物异源表达的方法。还提供了使用转座元件将大尺寸DNA分子引入异源宿主染色体的方法。还提供了新的色氨酸S衍生物。

3. 发明授权

US06509156B1 DNA cloning method relying on the E. coli recE/recT recombination system 有权
标题翻译：依赖大肠杆菌recE / recT重组系统的DNA克隆方法
公开(公告)号：US06509156B1
公开(公告)日：2003-01-21
申请号：US09555510
申请日：2000-06-05
申请人： Francis Stewart , Youming Zhang , Frank Buchholz
发明人： Francis Stewart , Youming Zhang , Frank Buchholz
IPC分类号： C12Q168
CPC分类号： C12N15/10 , C12N15/902
摘要： The invention relates to methods for cloning DNA molecules using recE/recT-mediated homologous recombination mechanism between at least two DNA molecules where one DNA molecule is a circular or linear DNA molecule and the second DNA molecule is a circular DNA molecule, and the second DNA molecule contains two regions with sequence homology to the first DNA molecule. Competent cells and vectors are also described.
摘要翻译：本发明涉及使用在至少两个DNA分子之间的recE / recT介导的同源重组机制克隆DNA分子的方法，其中一个DNA分子是圆形或线性DNA分子，第二DNA分子是环状DNA分子，第二DNA 分子含有与第一个DNA分子具有序列同源性的两个区域。还描述了感受态细胞和载体。

4. 发明授权

US07736851B2 DNA cloning method 有权
标题翻译： DNA克隆方法
公开(公告)号：US07736851B2
公开(公告)日：2010-06-15
申请号：US10842534
申请日：2004-05-11
申请人： Francis Stewart , Youming Zhang , Frank Buchholz
发明人： Francis Stewart , Youming Zhang , Frank Buchholz
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12N15/10 , C12N15/902
摘要： The invention relates to methods for cloning DNA molecules using recE/recT-mediated homologous recombination mechanism between at least two DNA molecules where one DNA molecule is a circular or linear DNA molecule and the second DNA molecule is a circular DNA molecule, and the second DNA molecule contains two regions with sequence homology to the first DNA molecule. Competent cells and vectors are also described.
摘要翻译：本发明涉及使用在至少两个DNA分子之间的recE / recT介导的同源重组机制克隆DNA分子的方法，其中一个DNA分子是圆形或线性DNA分子，第二DNA分子是环状DNA分子，第二DNA 分子含有与第一个DNA分子具有序列同源性的两个区域。还描述了感受态细胞和载体。

5. 发明授权

US06787316B2 DNA cloning method 有权
标题翻译： DNA克隆方法
公开(公告)号：US06787316B2
公开(公告)日：2004-09-07
申请号：US10231013
申请日：2002-08-30
申请人： Francis Stewart , Youming Zhang , Frank Buchholz
发明人： Francis Stewart , Youming Zhang , Frank Buchholz
IPC分类号： C12Q168
CPC分类号： C12N15/10 , C12N15/902
摘要： The invention relates to methods for cloning DNA molecules using recE/recT-mediated homologous recombination mechanism between at least two DNA molecules where one DNA molecule is a circular or linear DNA molecule and the second DNA molecule is a circular DNA molecule, and the second DNA molecule contains two regions with sequence homology to the first DNA molecule. Competent cells and vectors are also described.
摘要翻译：本发明涉及使用在至少两个DNA分子之间的recE / recT介导的同源重组机制克隆DNA分子的方法，其中一个DNA分子是圆形或线性DNA分子，第二DNA分子是环状DNA分子，第二DNA 分子含有与第一个DNA分子具有序列同源性的两个区域。还描述了感受态细胞和载体。

6. 发明授权

US07000900B1 Hydraulically powered reel lift 失效
标题翻译：液压动力卷轴提升
公开(公告)号：US07000900B1
公开(公告)日：2006-02-21
申请号：US10754974
申请日：2004-01-09
申请人： Robert D. McKenney , Francis Stewart , Terry Hawkins
发明人： Robert D. McKenney , Francis Stewart , Terry Hawkins
IPC分类号： B60P1/48
CPC分类号： B66F3/08 , B65H49/32 , B65H49/325 , B66F7/0641 , B66F7/08
摘要： A portable reel jack for supporting and lifting large spools of heavy cable, wire and the like includes a hydraulic lift with an extendable piston rod. A stanchion that is pivotally connected to the piston rod carries a spool support and remains vertically oriented during movement of the piston rod relative to the cylinder. Moreover, when the piston rod moves vertically away from the base in a lifting motion, then the stanchion moves laterally away from the hydraulic cylinder. As the spool is being lifted off of the ground by the hydraulic jack, the load-bearing location of the rod that carries the spool moves increasingly farther away from where the operator is standing to operate the lifting action of the portable reel jack. Additionally, the spool support has the flexibility of three different height elevations for carrying the rod on which the spool is in turn carried. This three-tiered configuration permits the operator to select the lowest possible support position for the rod and thus enables the vertical movement of the jack's piston rod to be minimized for a spool of any given circumference.
摘要翻译：用于支撑和提升重型电缆，电线等的大型线轴的便携式卷轴千斤顶包括具有可伸缩活塞杆的液压升降机。枢转地连接到活塞杆的支柱承载卷轴支撑并且在活塞杆相对于气缸的运动期间保持垂直定向。此外，当活塞杆在升降运动中从基座垂直移动时，支柱横向移动离开液压缸。当阀芯通过液压千斤顶被抬离地面时，承载卷轴的杆的承载位置越来越远离操作者站立以操作便携式卷轴千斤顶的提升动作。此外，卷筒支撑件具有三个不同的高度高度的灵活性，用于承载在其上承载线轴的杆。这种三层构造允许操作者选择杆的最低可能的支撑位置，从而使得千斤顶的活塞杆的垂直运动能够最小化为任何给定周长的线轴。

7. 发明授权

US6040430A Nucleic acid encoding site specific recombinase and nuclear receptor fusion protein 失效
标题翻译：核酸编码位点特异性重组酶和核受体融合蛋白
公开(公告)号：US6040430A
公开(公告)日：2000-03-21
申请号：US564264
申请日：1995-12-26
申请人： Francis Stewart
发明人： Francis Stewart
IPC分类号： C12N15/09 , C07K2/00 , C07K14/195 , C07K14/705 , C07K14/72 , C07K19/00 , C12N5/10 , C12N9/00 , C12N15/12 , C12N15/62 , C07K14/435
CPC分类号： C07K14/721 , C12N9/00 , C07K2319/00
摘要： An invention is described that permits the regulation of recombination in cells, organisms or appropriate cell-free systems. The invention involves creating fusion proteins between recombinase proteins, or components of recombinase systems, and ligand binding domains derived from nuclear receptors. The fusion proteins show little recombinase activity in the absence of the ligand that binds to the ligand binding domain. Upon binding of the ligand, recombinase activity is induced. The invention provides a practical means to regulate recombination in cells and organisms and, by linking ligand binding to recombination, provides a simple means whereby ligand binding can be measured as recombination achieved.
摘要翻译： PCT No.PCT / EP94 / 02088 Sec。 371日期1995年12月26日第 102（e）日期1995年12月26日PCT提交1994年6月28日PCT公布。出版物WO95 / 00555 日期1995年1月5日描述了允许在细胞，生物体或适当的无细胞系统中调节重组的发明。本发明涉及在重组酶蛋白或重组酶系统的组分和衍生自核受体的配体结合结构域之间产生融合蛋白。融合蛋白在不存在与配体结合结构域结合的配体的情况下显示出很少的重组酶活性。在配体结合后，诱导重组酶活性。本发明提供了调控细胞和生物体中的重组以及通过连接配体结合重组的实用手段，提供了一种简单的手段，由此可以通过实现的重组来测量配体结合。

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