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1. 发明授权

US08728820B2 Method of nucleic acid recombination 有权
标题翻译：核酸重组方法
公开(公告)号：US08728820B2
公开(公告)日：2014-05-20
申请号：US12918134
申请日：2009-02-20
申请人： Marcello Maresca , Axel Steffen Erler , Jun Fu , Philipp Martin Seibert , Adrian Francis Stewart , Youming Zhang
发明人： Marcello Maresca , Axel Steffen Erler , Jun Fu , Philipp Martin Seibert , Adrian Francis Stewart , Youming Zhang
IPC分类号： C12N15/90 , C12N15/09
CPC分类号： C12N15/1082
摘要： The invention provides a method for inserting a single stranded replacement nucleic acid into a target nucleic acid, the method comprising the steps of: a) generating a single stranded replacement nucleic acid from a double stranded nucleic acid, wherein the double stranded nucleic acid is adapted at one or both of its 5′ ends such that preferential degradation of one strand and/or strand separation generates the single stranded replacement nucleic acid, wherein the single stranded replacement nucleic acid comprises a 5′ region that is identical to sequence on the target nucleic acid, a 3′ region that is identical to sequence on the target nucleic acid and optionally a replacement region between the 5′ and 3′ regions that is not identical to sequence on the target nucleic acid, b) exposing the target nucleic acid to the single stranded replacement nucleic acid under conditions suitable for recombination to occur between the single stranded replacement nucleic acid and the target nucleic acid, and c) selecting a target nucleic acid whose sequence has been altered by inclusion of said single stranded replacement nucleic acid. Other methods for modifying target nucleic acids are also provided.
摘要翻译：本发明提供了将单链置换核酸插入靶核酸的方法，所述方法包括以下步骤：a）从双链核酸产生单链置换核酸，其中双链核酸适应在其5'末端的一个或两个上，使得一条链和/或链分离的优先降解产生单链置换核酸，其中单链置换核酸包含与靶核酸上的序列相同的5'区酸，与靶核酸上的序列相同的3'区，以及任选地在与靶核酸上的序列不相同的5'和3'区之间的置换区，b）将靶核酸暴露于在单链置换核酸和焦油之间发生适合重组的条件下，单链置换核酸得到核酸，和c）选择其核苷酸序列已经通过包含所述单链置换核酸而被改变。还提供了用于修饰靶核酸的其它方法。

2. 发明申请

US20110165630A1 Method of Nucleic Acid Recombination 有权
标题翻译：核酸重组方法
公开(公告)号：US20110165630A1
公开(公告)日：2011-07-07
申请号：US12918134
申请日：2009-02-20
申请人： Marcello Maresca , Axel Steffen Erler , Jun Fu , Philipp Martin Seibert , Adrian Francis Stewart , Youming Ahang
发明人： Marcello Maresca , Axel Steffen Erler , Jun Fu , Philipp Martin Seibert , Adrian Francis Stewart , Youming Ahang
IPC分类号： C12P19/34 , C07H1/00 , C12N1/21 , C12N15/70 , C07K2/00 , C12N15/87
CPC分类号： C12N15/1082
摘要： The invention provides a method for inserting a single stranded replacement nucleic acid into a target nucleic acid, said method comprising the steps of: a) generating a single stranded replacement nucleic acid from a double stranded nucleic acid, wherein the double stranded nucleic acid is adapted at one or both of its 5′ ends such that preferential degradation of one strand and/or strand separation generates the single stranded replacement nucleic acid, wherein the single stranded replacement nucleic acid comprises a 5′ region that is identical to sequence on the target nucleic acid, a 3′ region that is identical to sequence on the target nucleic acid and optionally a replacement region between the 5′ and 3′ regions that is not identical to sequence on the target N nucleic acid, b) exposing the target nucleic acid to the single stranded replacement nucleic acid under conditions suitable for recombination to occur between the single stranded replacement nucleic acid and the target nucleic acid, and c) selecting a target nucleic acid whose sequence has been altered by inclusion of said single stranded replacement nucleic acid. Other methods for modifying target nucleic acids are also provided.
摘要翻译：本发明提供了将单链置换核酸插入靶核酸的方法，所述方法包括以下步骤：a）从双链核酸产生单链置换核酸，其中双链核酸适应在其5'末端的一个或两个上，使得一条链和/或链分离的优先降解产生单链置换核酸，其中单链置换核酸包含与靶核酸上的序列相同的5'区酸，与靶核酸上的序列相同的3'区域，以及任选地在与目标N核酸上的序列不相同的5'和3'区域之间的置换区域，b）将靶核酸暴露于单链置换核酸在适于重组的条件下发生在单链置换核酸和靶核酸，以及c）通过包含所述单链置换核酸来选择其序列已被改变的靶核酸。还提供了用于修饰靶核酸的其它方法。

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