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    • 1. 发明公开
    • 고추 작물을 위한 길항 미생물 트라이코델마 하르지아눔을이용한 미생물 제제
    • 用于使用具有拮抗活性的TRICHODERMA HARZIANUM的大戟IGHT IGHT OF ER ER ER ER ER FOR FOR ITY ITY ITY ITY ITY ITY ITY ITY ITY ITY ITY ITY ITY ITY ITY ITY ITY ITY ITY
    • KR1020070122181A
    • 2007-12-28
    • KR1020070062516
    • 2007-06-25
    • 대구대학교 산학협력단
    • 이용세장태현송치현
    • A01N63/00C12N1/20C12N1/00
    • A01N63/04A01N25/12A01N63/02C12N1/14C12N1/20C12R1/125C12R1/885
    • A composition comprising a microorganism Trichoderma harzianum is provided to control pepper blight caused by Capsicum annuum L. in an antagonistic manner without environmental damage. A method for manufacturing a composition for biological control of pepper blight comprises the steps of: inoculating Trichoderma harzianum in potato dextrose medium containing corn starch, glucose and inorganic nutrients, culturing it at 26-28 deg.C for 48-96 hours to obtain a cultured medium(A), culturing Bacillus subtilis in the same medium above to obtain a cultured medium(B), inoculating and culturing the cultured medium (A) and (B) in a medium containing zeolite, white carbon, magnesium hydroxide, diatomite, talc, corn starch, glucose, KH2PO4, EDTA iron, zinc sulfate and water; spreading the cultured products on the fabric and culturing them at 20-28 deg.C for 5-7 days; cutting the cultured product along the lines with a distance of 1-3 cm when white hyphae of T. harzianum are generated on the surface to promote spore formation, and further culturing them for 3-7 days; drying the cultured products at 35-40 deg.C for 5-7 days; and pulverizing the dried cultured products.
    • 提供了包含微生物哈氏木霉的组合物,以对抗方式控制辣椒疫霉引起的胡椒枯病,无环境损害。 一种用于制备辣椒疫病生物防治组合物的方法,包括以下步骤:将马铃薯木霉接种在含有玉米淀粉,葡萄糖和无机营养物的马铃薯葡萄糖培养基中,在26-28℃培养48-96小时,得到 培养基(A),在上述相同的培养基中培养枯草芽孢杆菌以获得培养基(B),在含有沸石,白炭黑,硅藻土,硅藻土的培养基中接种培养培养基(A)和(B) 滑石粉,玉米淀粉,葡萄糖,KH 2 PO 4,EDTA铁,硫酸锌和水; 将培养的产品铺展在织物上并在20-28℃下培养5-7天; 在表面产生T. harzianum的白菌丝以促进孢子形成,并进一步培养3-7天,沿着距离1-3cm的线切割培养的产物。 将培养物在35-40℃干燥5-7天; 并将干燥的培养产物粉碎。
    • 2. 发明公开
    • 발효 인삼추출물의 제조방법
    • 制备发酵金山提取物的方法
    • KR1020130003944A
    • 2013-01-09
    • KR1020110065605
    • 2011-07-01
    • 에스케이바이오랜드 주식회사주식회사 엘지생활건강
    • 박지호최지휘유희종김기호이성구박형국이미경이헌식유은정윤혜경
    • A23L33/00A23L19/00A23L2/38
    • A23L33/105A23L2/38A23L2/385A23V2002/00A23V2250/2124C12R1/225C12R1/885
    • PURPOSE: A manufacturing method of a fermented ginseng extract with increased contents of ginsenoside Rd, Rg30r, and Rk1 is provided. CONSTITUTION: A ginseng extract is produced from ginseng. The ginseng extract is fermented with lactobacillus. The fermentation by the lactobacillus is processed with high pressure emulsification to destroy lactobacillus cells. Trichoderma reesei is inoculated from the high pressure emulsification processed and fermented lactobacillus, and cultivated to obtain a fermented ginseng extract. The fermented ginseng extract is heated for 20-40 minutes at 110-125 deg. C. The lactobacillus may be Lactobacillus fermentum. The high pressure emulsification process is performed at 1,300-1,600 bar for 2-4 times. The cultivation of Trichoderma reesei is performed for 3-5 days at 25-30 deg. C. The fermented ginseng extract has two or more times higher content of ginsenoside Rd, Rg3-r, and Rk1 compared to conventional ginseng extracts. [Reference numerals] (AA) Ginseng extract; (BB) Lactobacillus incubation; (CC) Processing high pressure emulsifier; (DD) Tricoderma Receei incubation; (EE) Sterilization; (FF) Enrichment
    • 目的:提供人参皂苷Rd,Rg30r和Rk1含量增加的发酵人参提取物的制备方法。 构成:人参提取物是由人参制成的。 人参提取物用乳杆菌发酵。 用高压乳化法处理乳杆菌的发酵以破坏乳杆菌细胞。 将里氏木霉从高压乳化处理和发酵乳杆菌中接种,培养得到发酵人参提取物。 将发酵的人参提取物在110-125度加热20-40分钟。 C.乳杆菌可以是发酵乳杆菌(Lactobacillus fermentum)。 高压乳化过程在1,300-1,600巴下进行2-4次。 里氏木霉的培养在25-30度进行3-5天。 C.与常规人参提取物相比,发酵人参提取物的人参皂苷Rd,Rg3-r和Rk1含量高出两倍以上。 (参考号)(AA)人参提取物; (BB)培养的乳杆菌; (CC)加工高压乳化剂; (DD)Tricoderma Receei孵育; (EE)灭菌; (FF)浓缩
    • 6. 发明公开
    • 트리코데르마 아트로비리데 OB―1 균주를 이용한 미생물제제
    • 使用TRICHODERMA ATROVIRIDE OB-1的微生物代理
    • KR1020120104875A
    • 2012-09-24
    • KR1020110022547
    • 2011-03-14
    • (주)오비트
    • 이동현이극래이준석박기병문준혁
    • C12N1/14A01N63/04C12R1/885
    • A01N63/04C12R1/885
    • PURPOSE: A Trichoderma atroviride strain and a microorganism formulation using the same are provided to ensure resistance to various plant pathogens. CONSTITUTION: A microorganism formulation contains Trichoderma atroviride OB-1(deposit number KCCM 11173P) or culture thereof as an active ingredient. The microorganism formulation also contains agriculturally acceptable carrier and is used for promoting plant growth or preventing plant diseases. The plant diseases are Botrytis cinerea, Rhizoctonia solani, Collectotrichum acutatum, or Fusarium oxysporum. [Reference numerals] (AA) Growth length(cm); (BB) Before irradiation; (CC) First irradiation; (DD) Second irradiation; (EE) Third irradiation; (FF) Fourth irradiation; (GG) Fifth irradiation; (HH) Sixth irradiation; (II) Seventh irradiation; (JJ) Eighth irradiation; (KK) Ninth irradiation; (LL) Tenth irradiation; (MM) Dipping treatment; (NN) Surface soil treatment; (OO) Dipping treatment + surface soil treatment; (PP) Non-treated group
    • 目的:提供木霉霉素菌株和使用其的微生物制剂以确保对各种植物病原体的抗性。 构成:微生物制剂含有叶绿体木霉OB-1(保藏号KCCM 11173P)或其培养物作为活性成分。 微生物制剂还含有农业上可接受的载体,用于促进植物生长或预防植物病害。 植物病害是灰葡萄孢(Botrytis cinerea),立枯丝核菌(Rhizoctonia solani),,,(Collectotrichum acutatum)或尖孢镰刀菌(Fusarium oxysporum)。 (AA)生长长度(cm); (BB)照射前; (CC)第一次照射; (DD)第二次照射; (EE)第三次照射; (FF)第四次照射; (GG)第五次照射; (HH)第六次照射; (二)第七次照射; (JJ)第八次照射; (KK)第九次照射; (LL)第十次照射; (MM)浸渍处理; (NN)表土处理; (OO)浸渍处理+表土处理; (PP)未处理组
    • 9. 发明公开
    • 식물병원균 방제에 유용한 무독성 살균제조성물
    • 不挥发性生物杀虫剂用于植物病原体的繁殖
    • KR1020000036322A
    • 2000-07-05
    • KR1020000003114
    • 2000-01-22
    • 주식회사 그린월드
    • 황연성신영봉
    • A01N63/00
    • A01N63/04A01G9/00A01N63/02C12R1/06C12R1/125C12R1/39C12R1/66C12R1/80C12R1/885
    • PURPOSE: Nontoxic bio-pesticides exterminating plant pathogen are provided which replace chemical pesticides used for the diseases of grass of golf course and such like. CONSTITUTION: Antagonist microorganisms are isolated from soil of grass field. Fungi such as Trichoderma harzianum, Aspergillus niger and Penicillium frequentans (KCTC 0721BP) are identified and bacteria such as Bacillus subtilis, Pseudomonas fluorescenct and Athrobacter terrengens (KCTC 0720BP) are also identified. Fungi are preserved in PDA plate at 4°C and transferred at every 2 months. Long term stock is preserved in Silica gel and 7% of skim milk at desiccator. Mass culture of fungi is tried in the liquid medium consisted of yeast peptone, sodium acetate, glucose and antifoaming agent or YMB, or solid medium consisted of rice bran, wheat bran and powder of cow skin. Nutrient agar is used to preserve bacteria and transferred at every 1 month. Glycerol stock is used for long term stock of bacteria. After the cultivation, broth is diluted with water about 100 times and sprayed 7-10 times from spring to fall. The amount of chemical pesticide used is decreased about 80%.
    • 目的:提供灭菌植物病原体的无毒生物杀虫剂,替代高尔夫球场草药疾病等化学农药。 构成:拮抗剂微生物从草地土壤中分离。 鉴定了真菌如哈茨木霉,黑曲霉和青霉菌(KCTC 0721BP),还鉴定了枯草芽孢杆菌,荧光假单胞菌和铁杆菌属(KCTC 0720BP)等细菌。 真菌在4℃保存在PDA板上,每2个月转移。 长期储存保存在硅胶中,7%的脱脂奶在干燥器中保存。 在由酵母蛋白胨,乙酸钠,葡萄糖和消泡剂或YMB组成的液体培养基中,或者由米糠,麦麸和牛皮粉末组成的固体培养基中,尝试真菌的大量培养。 营养琼脂用于保存细菌,每1个月转移一次。 甘油原料用于长期储存细菌。 培养后,汤水用水稀释约100次,从春季喷施7-10次。 化学农药使用量减少约80%。