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3. 发明公开

KR1020070122181A 고추 작물을 위한 길항 미생물 트라이코델마 하르지아눔을이용한 미생물 제제 有权
标题翻译：用于使用具有拮抗活性的TRICHODERMA HARZIANUM的大戟IGHT IGHT OF ER ER ER ER ER FOR FOR ITY ITY ITY ITY ITY ITY ITY ITY ITY ITY ITY ITY ITY ITY ITY ITY ITY ITY ITY
公开(公告)号：KR1020070122181A
公开(公告)日：2007-12-28
申请号：KR1020070062516
申请日：2007-06-25
申请人： 대구대학교 산학협력단
发明人： 이용세 , 장태현 , 송치현
IPC分类号： A01N63/00 , C12N1/20 , C12N1/00
CPC分类号： A01N63/04 , A01N25/12 , A01N63/02 , C12N1/14 , C12N1/20 , C12R1/125 , C12R1/885
摘要： A composition comprising a microorganism Trichoderma harzianum is provided to control pepper blight caused by Capsicum annuum L. in an antagonistic manner without environmental damage. A method for manufacturing a composition for biological control of pepper blight comprises the steps of: inoculating Trichoderma harzianum in potato dextrose medium containing corn starch, glucose and inorganic nutrients, culturing it at 26-28 deg.C for 48-96 hours to obtain a cultured medium(A), culturing Bacillus subtilis in the same medium above to obtain a cultured medium(B), inoculating and culturing the cultured medium (A) and (B) in a medium containing zeolite, white carbon, magnesium hydroxide, diatomite, talc, corn starch, glucose, KH2PO4, EDTA iron, zinc sulfate and water; spreading the cultured products on the fabric and culturing them at 20-28 deg.C for 5-7 days; cutting the cultured product along the lines with a distance of 1-3 cm when white hyphae of T. harzianum are generated on the surface to promote spore formation, and further culturing them for 3-7 days; drying the cultured products at 35-40 deg.C for 5-7 days; and pulverizing the dried cultured products.
摘要翻译：提供了包含微生物哈氏木霉的组合物，以对抗方式控制辣椒疫霉引起的胡椒枯病，无环境损害。一种用于制备辣椒疫病生物防治组合物的方法，包括以下步骤：将马铃薯木霉接种在含有玉米淀粉，葡萄糖和无机营养物的马铃薯葡萄糖培养基中，在26-28℃培养48-96小时，得到培养基（A），在上述相同的培养基中培养枯草芽孢杆菌以获得培养基（B），在含有沸石，白炭黑，硅藻土，硅藻土的培养基中接种培养培养基（A）和（B）滑石粉，玉米淀粉，葡萄糖，KH 2 PO 4，EDTA铁，硫酸锌和水; 将培养的产品铺展在织物上并在20-28℃下培养5-7天; 在表面产生T. harzianum的白菌丝以促进孢子形成，并进一步培养3-7天，沿着距离1-3cm的线切割培养的产物。将培养物在35-40℃干燥5-7天; 并将干燥的培养产物粉碎。

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