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    • 2. 发明公开
    • 포도의 품종 판별용 스카 마커 및 이의 용도
    • 用于辨别花岗岩的标记标记及其用途
    • KR1020130078111A
    • 2013-07-10
    • KR1020110146881
    • 2011-12-30
    • 대한민국(농촌진흥청장)
    • 조강희신일섭김세희김정희황해성
    • C12N15/11C12Q1/68
    • C12Q1/6895C12Q2535/139
    • PURPOSE: A sequence characterized amplified region (SCAR) marker for determining the variety of grapes is provided to definitely distinguish the variety of grapes, to strengthen protection of plant variety right holders, and to be used in a research of molecular genetics and morphological characteristics of the variety. CONSTITUTION: A SCAR marker for determining the variety of grapes contains one or more selected from the group consisting of G509-243 (sequence number 35), GG05_281 (sequence number 36), GU19_283 (sequence number 37), G116_319 (sequence number 38), G507_334 (sequence number 39), G427_348 (sequence number 40), GU13_380 (sequence number 41), G427_382 (sequence number 42), G119_411 (sequence number 43), GV01_424 (sequence number 44), GW04_463 (sequence number 45), G169_515 (sequence number 46), G116_539 (sequence number 47), GV04_618 (sequence number 48), GV01_678 (sequence number 49), GG05_689 (sequence number 50), and GA07_775 (sequence number 51). The SCAR marker is acquired by random amplified polymorphic DNA (RAPD) analysis. A method for determining the variety of grapes comprises the steps of: isolating genome DNA from a grape; amplifying a target sequence by amplification using an oligonucleotide SCAR primer set and the genome DNA as a template; and detecting the amplified product.
    • 目的:提供用于确定葡萄品种的扩增区(SCAR)标记序列,以确定区分各种葡萄,加强对植物品种权利人的保护,并用于研究分子遗传学和形态学特征 品种。 构成:用于确定葡萄品种的SCAR标记含有选自G509-243(序列号35),GG05_281(序列号36),GU19_283(序列号37),G116_319(序列号38)中的一种或多种, ,G507_334(序列号39),G427_348(序列号40),GU13_380(序列号41),G427_382(序列号42),G119_411(序列号43),GV01_424(序列号44),GW04_463(序列号45) G169_515(序列号46),G116_539(序列号47),GV04_618(序列号48),GV01_678(序列号49),GG05_689(序列号50)和GA07_775(序列号51)。 通过随机扩增多态性DNA(RAPD)分析获得SCAR标记。 确定葡萄品种的方法包括以下步骤:从葡萄中分离基因组DNA; 使用寡核苷酸SCAR引物组和基因组DNA作为模板扩增靶序列; 并检测扩增产物。
    • 4. 发明公开
    • 담배 품종 판별용 RAPD 프라이머
    • 用于歧视烟草种类的随机放大多态性DNA引物
    • KR1020160086177A
    • 2016-07-19
    • KR1020150003588
    • 2015-01-09
    • 충북대학교 산학협력단
    • 이이조남수유제민김예진엄유리
    • C12Q1/68
    • C12Q1/6888C12Q1/686C12Q2535/139C12Q2600/13
    • 본발명은 RAPD(Random Amplified Polymorphic DNA) 분석에의한담배의품종판별방법및 그방법에사용되는프라이머에관한것으로, 보다구체적으로는서열번호 1 내지서열번호 14로이루어진군에서선택되는특정염기서열을갖는 RAPD 프라이머및 이를이용하여 PCR을수행하는것을포함하는담배의 DNA 다형성검출방법및 담배의품종판별방법에관한것이다. 본발명에따른담배품종판별용 RAPD 마커는담배의 DNA 다형성을효과적으로검출하여담배의 DNA 프로파일을작성하는데매우유용하게이용될수 있으며, 이를통해담배의유전자원을효율적으로평가함으로써신규유전자원도입시 기존보유자원과의중복성분석및 신규성확립을효과적으로수행할수 있으며, 담배의품종을판별할수 있는효과가있다. 또한, 국내에산재해있는토종품종(계통)간의구분을명확히할 수있을뿐만아니라이름없는품종들의신품종등록이가능하여우리의소중한유전자원을보호할수 있으며, 국내산과수입산의식별이가능해져국내담배시장의유통질서를확립하는데기여할수 있다.
    • 本发明涉及一种通过随机扩增多态性DNA(RAPD)分析区分烟草种类的方法及其用途。 更具体地,本发明涉及具有选自SEQ ID NO:1至SEQ ID NO:14的特定碱基序列的RAPD引物; 一种检测烟草DNA多态性的方法,其中该方法包括使用该方法进行PCR; 以及鉴别烟草种类的方法。 根据本发明,用于区分烟草种类的RAPD标记物有效地检测烟草的DNA多态性,并且可以非常有用于书写烟草的DNA谱。 因此,有效地评估了烟草的遗传来源,可以有效地分析引入的新型遗传源与常规保留资源的多样性,有效地建立新颖的遗传资源。 此外,可以区分烟草的种类。 此外,国内分布的本地物种可以明确分离,没有标题的物种可以登记为新物种。 因此,珍贵的遗传资源得到保护,国内物种和进口物种可以被鉴定出来。 因此,可以在国内烟草市场建立分销订单。
    • 5. 发明公开
    • 배 품종 식별용 SCAR 마커 및 그 용도
    • 用于在梨和其中使用的花卉鉴定的标记标记
    • KR1020120069446A
    • 2012-06-28
    • KR1020100131000
    • 2010-12-20
    • 대한민국(농촌진흥청장)
    • 조강희신일섭김정희김세희김대현허성김현란
    • C12N15/11C12Q1/68
    • C12Q1/6895C12Q2535/138C12Q2535/139C12Q2600/13
    • PURPOSE: A SCAR marker for distinguishing pear species is provided to accurately distinguish native species and introduced species. CONSTITUTION: A SCAR marker for distinguishing pear species contains: P123_279 primer set of an oligonucleotide of sequence numbers 1 and 2; P561_331 primer set of an oligonucleotide of sequence numbers 3 and 4; P561_372 primer set having an oligonucleotide of sequence numbers 5 and 6; PH02_405 primer set having an oligonucleotide of sequence numbers 7 and 8; PK15_410 primer set having an oligonucleotide of sequence numbers 9 and 10; PH15_452 primer set having an oligonucleotide of sequence numbers 11 and 12; PT16_472 primer set having an oligonucleotide of sequence numbers 13 and 14; PT06_509 prime set having an oligonucleotide of sequence numbers of sequence numbers 15 and 16; PN11_516 primer set having an oligonucleotide of sequence numbers 17 and 18; PK17_556 primer set having an oligonucleotide of sequence numbers 19 and 20; PN15_575 primer set having an oligonucleotide of sequence numbers 21 and 22; PT14_578 primer set having an oligonucleotide of sequence numbers 23 and 24; P270_593 primer set having an oligonucleotide of sequence numbers 25 and 26; PF07_594 primer set having an oligonucleotide of sequence numbers 27 and 28; PQ11_649 primer set having an oligonucleotide of sequence numbers 29 and 30; PK15_672 primer set having an oligonucleotide of sequence numbers 31 and 32; PK17_771 primer set having an oligonucleotide of sequence numbers 33 and 34; PK10_514 primer set having an oligonucleotide of sequence numbers 35 and 36; or PK15_616 primer set having an oligonucleotide of sequence numbers 37 and 38.
    • 目的:提供用于区分梨种的SCAR标记,以准确区分本地物种和引种物种。 构成:用于区分梨物种的SCAR标记物包含:序列号1和2的寡核苷酸的引物组:P123_279; P561_331序列号3和4的寡核苷酸的引物组; P561_372具有序列号5和6的寡核苷酸的引物组; 具有序列号7和8的寡核苷酸的PH02_405引物组; 具有序列号9和10的寡核苷酸的PK15_410引物组; 具有序列号11和12的寡核苷酸的PH15_452引物组; 具有序列号13和14的寡核苷酸的PT16_472引物组; PT06_509具有序列号为15和16的序列号的寡核苷酸的序列; 具有序列号17和18的寡核苷酸的PN11_516引物组; 具有序列号19和20的寡核苷酸的PK17_556引物组; 具有序列号21和22的寡核苷酸的PN15_575引物组; 具有序列号23和24的寡核苷酸的PT14_578引物组; P270_593具有序列号25和26的寡核苷酸的引物组; 具有序列号27和28的寡核苷酸的PF07_594引物组; PQ11_649具有序列号29和30的寡核苷酸的引物组; 具有序列号31和32的寡核苷酸的PK15_672引物组; 具有序列号33和34的寡核苷酸的PK17_771引物组; 具有序列号35和36的寡核苷酸的PK10_514引物组; 或具有序列号37和38的寡核苷酸的PK15_616引物组。
    • 6. 发明公开
    • 알에이피디 표지인자를 이용한 배추 뿌리혹병 저항성유전자 판별방법
    • 使用RAPD标记鉴定中华绒螯蟹基因耐药基因
    • KR1020080043164A
    • 2008-05-16
    • KR1020060111866
    • 2006-11-13
    • 대한민국(농촌진흥청장)
    • 조광수홍수영유승열우종규문지영장석우
    • C12N15/09C12N15/00C12Q1/68
    • C12N15/09C12N15/00C12Q1/6844C12Q2535/139
    • A method for identification of a clubroot resistance gene in Chinese cabbage is provided to reduce the identification time by performing identification with RAPD(randomly amplified polymorphic DNA) marker in a laboratory without a resistance examination process in the field, and screen a clubroot-resistant individual rapidly in a relatively small space. A clubroot resistance gene in Chinese cabbage is identified by confirming the genotype of Chinese cabbage clubroot resistance with RAPD marker, and selecting a resistant individual "CR-serona", wherein the resistance gene analysis of "CR-serona" is performed by discerning the resistance gene pattern of Chinese cabbage clubroot by using single spore-derived pathogenic bacteria(Rrace 4, Willinams method) and a group of F2, BC1P1 and BC1P2, and extracting DNAs from resistant and susceptible individuals from 230 individuals of F2 group and developing DNA marker closely associated with the resistance gene by BSA-RAPD(bulked segregant analysis-randomly amplified polymorphic DNA); and the RAPD marker is a DNA fragment amplified specifically by PCR(polymerase chain reaction) of Chinese cabbage genome by using RAPD primer(OPJ05).
    • 提供了一种用于鉴定大白菜的根茎抗性基因的方法,通过在实地没有电阻检查过程的RAPD(随机扩增多态性DNA)标记物进行鉴定来减少识别时间,并筛选出一个耐发丝的个体 迅速在相对较小的空间。 通过使用RAPD标记确认大白菜丝状杆菌耐药性的基因型,选择抗性个体“CR-serona”来鉴定大白菜中的种子抗性基因,其中通过鉴别抗性来进行“CR-serona”的抗性基因分析 通过使用单孢子源性病原菌(Rrace 4,Willinams法)和一组F2,BC1P1和BC1P2,从F2群体230个个体的抗性和易感个体提取DNA,并开发DNA标记 与BSA-RAPD的抗性基因相关(大量分离分析 - 随机扩增多态性DNA); RAPD标记是通过使用RAPD引物(OPJ05)的大白菜基因组的PCR(聚合酶链式反应)特异性扩增的DNA片段。
    • 7. 发明公开
    • 쥬빌리계 수박 호피 무늬 형질 선발용 DNA 마커
    • 用于选择水体的JUBILEE型条纹图案的DNA标记
    • KR1020160095212A
    • 2016-08-11
    • KR1020150015716
    • 2015-01-31
    • 부산대학교 산학협력단
    • 박영훈김용재
    • C12Q1/68
    • C12Q1/6895C12Q2535/139C12Q2600/13C12Q2600/156
    • 본발명은쥬빌리계수박호피무늬형질선발용 DNA 마커에관한것으로서, 상세하게는서열번호 1로표시되는 AT14-900 DNA 마커및 서열번호 2로표시되는 wsbin6-11 DNA 마커중 어느하나이상의 DNA 마커로이루어진쥬빌리-타입(Jubilee-type; JT) 호피무늬수박품종판별용마커를제공한다. 또한, 상기마커를증폭할수 있는프라이머세트, 쥬빌리-타입(Jubilee-type; JT) 호피무늬수박품종판별키트및 쥬빌리-타입(Jubilee-type; JT) 호피무늬수박품종판별방법을제공한다. 이러한 DNA 마커를이용하여쥬빌리계호피무늬품종을매우신속하고정확하게육성함으로써종자회사의육종프로그램에크게활용될수 있을것으로예상된다.
    • 本发明涉及一种用于选择禧年(JT)西瓜条纹图案特征的DNA标记。 特别地,本发明提供了一种用于确定JT条纹图案西瓜种类的标记,其中所述标记包括由SEQ ID NO:1表示的AT14-900 DNA标记和SEQ ID NO:1所示的wsbin6-11 DNA标记中的任何一个或多个 此外,提供了能够扩增标记的引物组,用于确定JT条纹图案西瓜种的试剂盒,以及用于确定JT条纹图案西瓜种的方法。 通过使用这样的DNA标记,JT条纹图谱种类非常迅速和准确地提高,因此预期DNA标记物将在种子公司的育种计划中得到很大的利用。
    • 10. 发明公开
    • 알에이피디법을 이용한 바이러스의 동정방법
    • 使用RAPD鉴定病毒的方法
    • KR1020120129279A
    • 2012-11-28
    • KR1020110047439
    • 2011-05-19
    • 한국외국어대학교 연구산학협력단
    • 홍진경명희준이규호조재창
    • C12Q1/04C12Q1/68C12N7/01
    • C12Q1/04C12Q1/6844C12Q2535/139
    • PURPOSE: A diagnosis method of virus using RAPD method is provided to simply and efficiently diagnose virus which do not have index gene which informs phylogenic location. CONSTITUTION: A diagnosis method of virus uses RAPD method(random amplified polymorphic DNA). A diagnosis method of virus comprises the following steps: amplifying DNA pieces of virus by applying RAPD PCR to virus which will be diagnosed; and comparing banding patterns of band by performing electrophoresis the amplified DNA pieces. A random primer manufacturing method used in RAPD PCR comprises the following steps: manufacturing primers comprised of 10 nucleotides by varying G+C content of primer base sequence and
    • 目的:提供使用RAPD方法的病毒诊断方法,简单有效地诊断不具有通知系统发生位置的指标基因的病毒。 构成:病毒诊断方法采用RAPD法(随机扩增多态性DNA)。 病毒的诊断方法包括以下步骤:通过将RAPD PCR应用于待诊断的病毒来扩增DNA片段; 并通过对扩增的DNA片段进行电泳来比较条带的条带模式。 在RAPD PCR中使用的随机引物制备方法包括以下步骤:通过改变引物碱基序列的G + C含量和两端的<= G来制造由10个核苷酸组成的引物; 检查在制造的底漆上是否存在发夹或二聚体纹理发生; 并对在计算机中计算的效率最高的引物序列(等级= 100 +(<= G(Dimer)X 1.8 + <= G(发夹)X 1.4))进行实验分析。