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    • 2. 发明公开
    • 배 품종 식별용 SCAR 마커 및 그 용도
    • 用于在梨和其中使用的花卉鉴定的标记标记
    • KR1020120069446A
    • 2012-06-28
    • KR1020100131000
    • 2010-12-20
    • 대한민국(농촌진흥청장)
    • 조강희신일섭김정희김세희김대현허성김현란
    • C12N15/11C12Q1/68
    • C12Q1/6895C12Q2535/138C12Q2535/139C12Q2600/13
    • PURPOSE: A SCAR marker for distinguishing pear species is provided to accurately distinguish native species and introduced species. CONSTITUTION: A SCAR marker for distinguishing pear species contains: P123_279 primer set of an oligonucleotide of sequence numbers 1 and 2; P561_331 primer set of an oligonucleotide of sequence numbers 3 and 4; P561_372 primer set having an oligonucleotide of sequence numbers 5 and 6; PH02_405 primer set having an oligonucleotide of sequence numbers 7 and 8; PK15_410 primer set having an oligonucleotide of sequence numbers 9 and 10; PH15_452 primer set having an oligonucleotide of sequence numbers 11 and 12; PT16_472 primer set having an oligonucleotide of sequence numbers 13 and 14; PT06_509 prime set having an oligonucleotide of sequence numbers of sequence numbers 15 and 16; PN11_516 primer set having an oligonucleotide of sequence numbers 17 and 18; PK17_556 primer set having an oligonucleotide of sequence numbers 19 and 20; PN15_575 primer set having an oligonucleotide of sequence numbers 21 and 22; PT14_578 primer set having an oligonucleotide of sequence numbers 23 and 24; P270_593 primer set having an oligonucleotide of sequence numbers 25 and 26; PF07_594 primer set having an oligonucleotide of sequence numbers 27 and 28; PQ11_649 primer set having an oligonucleotide of sequence numbers 29 and 30; PK15_672 primer set having an oligonucleotide of sequence numbers 31 and 32; PK17_771 primer set having an oligonucleotide of sequence numbers 33 and 34; PK10_514 primer set having an oligonucleotide of sequence numbers 35 and 36; or PK15_616 primer set having an oligonucleotide of sequence numbers 37 and 38.
    • 目的:提供用于区分梨种的SCAR标记,以准确区分本地物种和引种物种。 构成:用于区分梨物种的SCAR标记物包含:序列号1和2的寡核苷酸的引物组:P123_279; P561_331序列号3和4的寡核苷酸的引物组; P561_372具有序列号5和6的寡核苷酸的引物组; 具有序列号7和8的寡核苷酸的PH02_405引物组; 具有序列号9和10的寡核苷酸的PK15_410引物组; 具有序列号11和12的寡核苷酸的PH15_452引物组; 具有序列号13和14的寡核苷酸的PT16_472引物组; PT06_509具有序列号为15和16的序列号的寡核苷酸的序列; 具有序列号17和18的寡核苷酸的PN11_516引物组; 具有序列号19和20的寡核苷酸的PK17_556引物组; 具有序列号21和22的寡核苷酸的PN15_575引物组; 具有序列号23和24的寡核苷酸的PT14_578引物组; P270_593具有序列号25和26的寡核苷酸的引物组; 具有序列号27和28的寡核苷酸的PF07_594引物组; PQ11_649具有序列号29和30的寡核苷酸的引物组; 具有序列号31和32的寡核苷酸的PK15_672引物组; 具有序列号33和34的寡核苷酸的PK17_771引物组; 具有序列号35和36的寡核苷酸的PK10_514引物组; 或具有序列号37和38的寡核苷酸的PK15_616引物组。
    • 7. 发明公开
    • 사과 바이로이드 감염 진단을 위한 프라이머 및 이를이용한 진단방법
    • 用于诊断苹果皮肤病毒感染的准备和使用该方法进行诊断的方法
    • KR1020100010349A
    • 2010-02-01
    • KR1020080071277
    • 2008-07-22
    • 대한민국(농촌진흥청장)
    • 허성김현란김대일황정환강상조신용억
    • C12N15/10C12N15/29C12Q1/68
    • C12Q1/701C12Q2600/112C12Q2600/13
    • PURPOSE: A primer for diagnosing ASSVd(apple scar skin viroid) infection and a diagnosis method using the same are provided to accurately detect infection by ASSVd and improve diagnosis accuracy. CONSTITUTION: A primer for diagnosing ASSVd(apple scar skin viroid) comprises a sequence number 1 or 2. A method of NASBA(nucleic acid sequence based amplification) for diagnosing infection by ASSVd comprises: a first step of annealing a first primer on a sense RNA and synthesizing single-stranded DNA molecule; a second step of reacting RNase to DNA-RNA hybrid molecule to remove RNA; a third step of annealing a second primer on single-stranded DNA molecule and reacting reverse transcriptase to synthesize double stranded DNA; and a step of synthesizing antisense RNA mediating RNA polymerase with single-stranded DNA molecule as an template.
    • 目的:提供用于诊断ASSVd(苹果瘢痕皮肤病毒)感染的引物和使用其的诊断方法,以准确检测ASSVd感染并提高诊断准确性。 构成:用于诊断ASSVd(苹果瘢痕皮肤病毒)的引物包含序列号1或2.一种用于诊断ASSVd感染的NASBA(基于核酸序列的扩增)的方法包括:第一步骤,对第一引物进行退火 RNA和合成单链DNA分子; 使RNA酶与DNA-RNA杂交分子反应以除去RNA的第二步; 将第二引物退火在单链DNA分子上并使反转录酶反应合成双链DNA的第三步骤; 以单链DNA分子为模板合成反义RNA介导RNA聚合酶的步骤。
    • 8. 发明公开
    • 사과의 품종 판별용 SCAR 표지 및 이의 용도
    • 用于歧视苹果品种及其用途的标识标记
    • KR1020110074204A
    • 2011-06-30
    • KR1020090131107
    • 2009-12-24
    • 대한민국(농촌진흥청장)
    • 조강희허성김현란김정희신일섭
    • C12N15/29C12N15/11C12Q1/68
    • C12N15/11C12Q1/6844C12Q2600/158
    • PURPOSE: A SCAR(sequence characterized amplified region) marker for determining apples is provided to reinforce protection of apple species and quickly and accurately certify apple species. CONSTITUTION: A SCAR marker for determining apple species contains AL1_343(sequence number 35[SEQ ID NO:35]), AW15_368(sequence number 36), A330_424(sequence number 37), AL1_427(sequence number 38), AN11_433(sequence number 39), AT6_453 (sequence number 40), AG14_502 (sequence number 41), AN8_566 (sequence number 42), AK17_574 (sequence number 43), AK14_575 (sequence number 44), A408_592 (sequence number 45), AK17_653 (sequence number 46), AM16_708 (sequence number 47), AO4_711 (sequence number 48), AO4_779 (sequence number 49), AT14_789 (sequence number 50), or AL1_851(sequence number 51). A kit for determining apple species comprises: oligonucleotide SCAR primer set for amplifying SCAR marker; and reagent for amplification.
    • 目的:提供确定苹果的SCAR(序列特征扩增区)标记,以加强对苹果物种的保护,并快速,准确地认证苹果种类。 构成:用于确定苹果物种的SCAR标记包含AL1_343(序列号35 [SEQ ID NO:35]),AW15_368(序列号36),A330_424(序列号37),AL1_427(序列号38),AN11_433(序列号39 ),AT6_453(序列号40),AG14_502(序列号41),AN8_566(序列号42),AK17_574(序列号43),AK14_575(序列号44),A408_592(序列号45),AK17_653(序列号46) ,AM16_708(序列号47),AO4_711(序列号48),AO4_779(序列号49),AT14_789(序列号50)或AL1_851(序列号51)。 用于测定苹果种的试剂盒包括:用于扩增SCAR标记的寡核苷酸SCAR引物组; 和扩增试剂。