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    • 4. 发明公开
    • 입자 어레이의 제조 장치 및 제조 방법과 표적 물질의검출 방법
    • 用于制造珠粒阵列的装置和方法,其特征在于将表面上固定的探针的固定相的珠子提供给固体相基材的目标反应场,以及使用其来检测目标物质的方法
    • KR1020050024194A
    • 2005-03-10
    • KR1020040068931
    • 2004-08-31
    • 세이코 엡슨 가부시키가이샤
    • 다카기후미오
    • C12Q1/68
    • B01J19/0046B01J2219/00317B01J2219/00378B01J2219/00466B01J2219/005B01J2219/00545B01J2219/00554B01J2219/00563B01J2219/00574B01J2219/00576B01J2219/00596B01J2219/00722B01J2219/00725B01L3/0241B01L3/5085C40B40/06C40B40/10C40B60/14Y10T436/2575C12Q1/6834C12Q2563/149
    • PURPOSE: A device and method for manufacturing bead array, and a method for detecting a target substance using the same are provided, thereby accurately supplying the beads with fixed probes consisting of nucleic acids or proteins on the surface to a target reaction site of a solid-phase substrate. CONSTITUTION: The device(10) for manufacturing bead array comprises a substrate(11); a cartridge holder(carriage) (12) which moves in forward and backward in a X-axis direction; a table(13) which moves in forward and backward in a Y-axis direction which is perpendicular to the X-axis direction; a suction unit(14) for filling the discharging solution on the cartridge holder(12); and a carriage and table operating portion(15), wherein the cartridge holder(carriage) (12) comprises a discharging head; the discharging head comprises a liquid receiving means which receives liquid containing the biological material-fixed beads; a liquid discharging means which discharges the liquid containing the biological material-fixed beads on a target reaction site on the solid-phase substrate; the liquid discharging means uses ink-jet method; the liquid receiving means separately receives two kinds of different liquid; the biological material is nucleic acid or protein; the bead size is 0.02 to 10 micrometer; the solid-phase substrate is microtiter plate; and the reaction site is formed on the wells of the substrate surface.
    • 目的:提供珠粒阵列的制造装置和方法,以及使用该珠粒阵列的靶物质的检测方法,从而将由表面的核酸或蛋白质构成的固定探针精确地供给到固体的靶反应部位 相衬底。 构成:用于制造珠阵列的装置(10)包括基底(11); 一个在X轴方向前后移动的盒架(滑架)(12) 在与X轴方向垂直的Y轴方向上前后移动的工作台(13) 用于将排出溶液填充在盒保持器(12)上的抽吸单元(14); 以及托架和工作台操作部分(15),其中所述盒保持器(托架)(12)包括排放头; 排出头包括接收含有生物材料固定珠的液体的液体接收装置; 在固相基板上的靶反应部位上排出含有生物材料固定珠的液体的液体排出装置; 液体排出装置使用喷墨方法; 液体接收装置分别接收两种不同的液体; 生物材料是核酸或蛋白质; 珠粒尺寸为0.02至10微米; 固相底物为微量滴定板; 并且反应位点形成在基材表面的孔上。