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1. 发明授权

KR101882740B1 젖산의 고효율 생산을 위한 변형 미생물 有权
公开(公告)号：KR101882740B1
公开(公告)日：2018-07-27
申请号：KR1020120061819
申请日：2012-06-08
申请人： 삼성전자주식회사 , 성균관대학교산학협력단
发明人： 정순천 , 구현민 , 김재영 , 김지은 , 김진우 , 박영경 , 이소영 , 조화영 , 권대혁 , 박재찬
IPC分类号： C12N15/52 , C12P7/56 , C12N15/81 , C12N15/74
摘要： 젖산의고효율생산을위한변형미생물, 상기변형미생물을제작하기위한발현벡터, 및상기변형미생물을이용한젖산의생산방법이개시된다. 상기변형미생물은산성조건하에서도고효율로젖산을생산할수 있다.

2. 发明公开

KR1020100119018A 리그노셀룰로오스계 바이오매스의 전처리 방법 无效
标题翻译：基于木质素的生物质的预处理方法
公开(公告)号：KR1020100119018A
公开(公告)日：2010-11-09
申请号：KR1020090037915
申请日：2009-04-30
申请人： 삼성전자주식회사 , 성균관대학교산학협력단
发明人： 조화영 , 김진우 , 박재찬 , 박성민 , 심상준 , 한세종
IPC分类号： C08B1/08 , C12P7/04 , C10G3/00 , C12R1/865
CPC分类号： C12P7/10 , C08H8/00 , C12P2201/00 , C13K1/02 , Y02E50/16 , Y02E50/343 , Y02P30/20 , C08B1/08 , C10G3/00 , C12P7/04 , C12R1/865
摘要： PURPOSE: A pretreatment method of a lignocellulose-based biomass is provided to obtain cellulose with high purity and hemicelluloses with the high yield, by extracting lignin first. CONSTITUTION: A pretreatment method of a lignocellulose-based biomass comprises the following steps: adding a solvent dissolving lignin to the lignocellulose-based biomass containing the lignin, hemicelluloses, and cellulose, to extract the lignin from the biomass(S1,S2); and adding an ionic solution to the biomass without the lignin, to extract the hemicelluloses and the cellulose(S3). The solvent dissolving the lignin is an alkaline solvent.
摘要翻译：目的：提供木质纤维素基生物质的预处理方法，首先通过提取木质素，以高产率获得高纯度的纤维素和半纤维素。构成：木质纤维素基生物质的预处理方法包括以下步骤：向含木质素，半纤维素和纤维素的木质纤维素基生物质中加入溶解木质素的溶剂，从生物质提取木质素（S1，S2）; 并将离子溶液加入到没有木质素的生物质中，以提取半纤维素和纤维素（S3）。溶解木质素的溶剂是碱性溶剂。

3. 发明公开

KR1020130001121A 젖산의 고효율 생산을 위한 변형 미생물 审中-实审
标题翻译：用于高效生产乳酸的改性微生物
公开(公告)号：KR1020130001121A
公开(公告)日：2013-01-03
申请号：KR1020120061819
申请日：2012-06-08
申请人： 삼성전자주식회사 , 성균관대학교산학협력단
发明人： 정순천 , 구현민 , 김재영 , 김지은 , 김진우 , 박영경 , 이소영 , 조화영 , 권대혁 , 박재찬
IPC分类号： C12N1/19 , C12N1/21 , C12N15/53 , C12P7/56
摘要： PURPOSE: A modified microorganism for producing lactic acids and a method for producing lactic acids are provided to produce lactic acids with high efficiency under an acidic condition. CONSTITUTION: A modified microorganism for producing lactic acids with high efficiency has LDH activity of Pelodiscus sinensis japonicus, Ornithorhynchus anatinus, Tursiops truncates, and Rattus norvegicus. The modified microorganism is yeast or bacteria. The modified microorganism is E.coli or Kluyveromyces marxianus. The modified microorganism produces lactic acids with 12.2% or more of glucose. An expression vector for producing the modified microorganism comprises: a replication origin; a promoter; a polynucleotide; and a terminator. A method for producing lactic acids comprises: a step of culturing the modified microorganism in a medium containing glucose; and a step of collecting lactic acids from the culture.
摘要翻译：目的：提供用于生产乳酸的改性微生物和生产乳酸的方法，以在酸性条件下高效生产乳酸。构成：用于高效生产乳酸的改良微生物具有日本芍药，鸟腥草，Tur ps属，and and属的LDH活性。修饰的微生物是酵母或细菌。修饰的微生物是大肠杆菌或马克斯克鲁维酵母（Kluyveromyces marxianus）。经修饰的微生物产生具有12.2％或更多葡萄糖的乳酸。用于产生修饰微生物的表达载体包括：复制起点; 启动子多核苷酸; 和终结者。制备乳酸的方法包括：在含有葡萄糖的培养基中培养经修饰的微生物的步骤; 以及从培养物中收集乳酸的步骤。

4. 发明公开

KR1020130001103A 젖산의 고효율 생산을 위한 변형 미생물 无效
标题翻译：用于高效生产乳酸的改性微生物
公开(公告)号：KR1020130001103A
公开(公告)日：2013-01-03
申请号：KR1020110139520
申请日：2011-12-21
申请人： 삼성전자주식회사 , 성균관대학교산학협력단
发明人： 정순천 , 구현민 , 김재영 , 김지은 , 김진우 , 박영경 , 이소영 , 조화영 , 권대혁 , 박재찬
IPC分类号： C12N15/63 , C12N1/21 , C12N15/53 , C12P7/56
摘要： PURPOSE: A modified microorganism for producing lactic acids is provided to prepare lactic acids with high efficiency under an acidic condition. CONSTITUTION: A modified microorganism for producing lactic acids has a lactate dehydrogenase(LDH) activity of Pelodiscus sinensis japonicus, Ornithorhynchus anatinus, Tursiops truncates, or Rattus norvegicus. The modified microorganism is Escherichia sp. or Kluyveromyces sp. The modified microorganism produces lactic acids with 34% or more of glucose. An expression vector contains: a replication origin for constructing the modified microroganisms; a promoter; a polynucleotide coding LDH activation of one or more species selected from the group consisting of Pelodiscus sinensis japonicus, Ornithorhynchus anatinus, Tursiops truncates, or Rattus norvegicus; and a terminator. The replication origin is ARS/CEN replication origin.
摘要翻译：目的：提供一种用于生产乳酸的改性微生物，用于在酸性条件下高效制备乳酸。构成：用于生产乳酸的改良微生物具有日本芍药（Ornisorhynchus anatinus，Tursiops truncates）或褐藻（Rattus norvegicus）的乳酸脱氢酶（LDH）活性。修饰的微生物是大肠杆菌属或克鲁维酵母属经修饰的微生物产生具有34％以上葡萄糖的乳酸。表达载体包含：用于构建修饰的微结构的复制起点; 启动子编码一种或多种选自以下的物质的LDH活化的多核苷酸：日本Pe us，鸟，us us us，，，;;;;;;;;;;;;;;;;;; 和终结者。复制起点是ARS / CEN复制来源。

5. 发明公开

KR1020150115289A 알데히드 데히드로게나제 변이체, 상기 변이체를 코딩하는 폴리뉴클레오티드, 상기 폴리뉴클레오티드를 포함하는 벡터 및 미생물, 및 이를 이용한 1,4-부탄디올의 생산방법 审中-实审
标题翻译：代谢脱氢酶突变体，编码突变体的多核苷酸，载体和具有多核苷酸的微生物，以及使用其制备1,4-丁二醇的方法
公开(公告)号：KR1020150115289A
公开(公告)日：2015-10-14
申请号：KR1020140040064
申请日：2014-04-03
申请人： 삼성전자주식회사
发明人： 정유경 , 박진환 , 김지은 , 조화영 , 조광명
IPC分类号： C12N9/04 , C12N15/53 , C12N1/21 , C12P7/16
CPC分类号： C12N9/0008 , C12N9/1025 , C12P7/18 , C12Y102/01003 , C12Y102/0101 , C12Y102/04001 , C12Y108/01004 , C12Y203/03001
摘要： 알데히드데히드로게나제변이체, 상기변이체를코딩하는폴리뉴클레오티드, 상기폴리뉴클레오티드를포함하는벡터및 미생물, 및이를이용한 1,4-부탄디올의생산방법에관한것이다.
摘要翻译：本发明涉及醛脱氢酶突变体，编码突变体的多核苷酸，载体和包含该多核苷酸的微生物，以及使用其制备1,4-丁二醇的方法。醛脱氢酶突变体具有催化从4-羟基丁酰辅酶A向4-羟基丁醛转化的活性。

6. 发明公开

KR1020130094115A 1,4-부탄디올의 생산을 위한 변형 미생물 无效
标题翻译：用于生产1,4-丁二醇的改性微生物
公开(公告)号：KR1020130094115A
公开(公告)日：2013-08-23
申请号：KR1020120015526
申请日：2012-02-15
申请人： 삼성전자주식회사
发明人： 이영민 , 구현민 , 김재영 , 김진우 , 박재찬 , 박준성 , 조화영 , 이평천
IPC分类号： C12N1/21 , C12P7/16 , C12N15/52 , C12N15/63
CPC分类号： C12P7/18 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12N9/1217 , C12N9/13 , C12N9/88 , C12Y101/01001 , C12Y101/01016 , C12Y102/01016 , C12Y102/04002 , C12Y203/01019 , C12Y207/02007 , C12Y208/03005 , C12Y401/01071
摘要： PURPOSE: A deformed microorganism for production of 1,4-butanediol is provided to produce 1,4-BDO by a biological producing process. CONSTITUTION: A deformed microorganism for production of 1.4-butanediol comprises: an activation converting alpha-ketoglutarate or succinate into 4-hydroxybutyril-CoA ("4-HB-CoA"); and an activation converting the 4-HB-CoA into 1,4-butanediols ("1,4-BDO"). The activation converting the 4-HB-CoA into the 1,4-BDO is coded by more than one kind of genes selected from a group comprising adh1, yiaY, adh4, adhB, mdh, eutG, fucO, dhaT, aldA, eutE, adhE1, adhE2 and adh2.
摘要翻译：目的：提供用于生产1,4-丁二醇的变形微生物，以通过生物制备方法生产1,4-BDO。构成：用于生产1,4-丁二醇的变形的微生物包括：将α-酮戊二酸或琥珀酸转化成4-羟基丁酰辅酶A（“4-HB-CoA”）的活化; 和将4-HB-CoA转化为1,4-丁二醇（“1,4-BDO”）的活化。将4-HB-CoA转化为1,4-BDO的激活由选自包含adh1，yyY，adh4，adhB，mdh，eutG，fucO，dhaT，aldA，eutE， adhE1，adhE2和adh2。

7. 发明公开

KR1020130000688A 젖산-생산 균주의 고속 선별 방법 无效
标题翻译：乳酸生产微生物的高通量筛选
公开(公告)号：KR1020130000688A
公开(公告)日：2013-01-03
申请号：KR1020110061366
申请日：2011-06-23
申请人： 삼성전자주식회사
发明人： 조화영 , 유병조 , 박재찬 , 박성민 , 구현민 , 이주영
IPC分类号： C12Q1/04 , C12N1/20 , C12N1/14 , C12P7/56
CPC分类号： G01N21/80 , C12Q1/025
摘要： PURPOSE: A high throughput screening system and a method of lactic acid-producing strains using two or more pH indicators are provided to quickly and accurately screen strains. CONSTITUTION: A high throughput screening system of lactic acid-producing strains contains the strain, a medium, and two or more pH indicators. The strains are a wild type strain, a mutant strain, and a recombinant strain. The pH indicators contain bromocresol green and methyl red in a ratio of 2:1-10:1. A method for screening the lactic acid-producing strains comprises: a step of adding the pH indicators to a medium; a step of culturing the strains; and a step of observing color change of the pH indicators, and measuring lactic acid production quantity.
摘要翻译：目的：提供高通量筛选系统和使用两种或多种pH指示剂的乳酸生产菌株的方法，以快速准确筛选菌株。构成：乳酸生产菌株的高通量筛选系统含有菌株，培养基和两种或多种pH指示剂。菌株是野生型菌株，突变菌株和重组菌株。 pH指标含有溴甲酚绿和甲基红，比例为2：1-10：1。筛选乳酸生产菌株的方法包括：将pH指示剂加入到培养基中的步骤; 培养菌株的一个步骤; 以及观察pH指示剂的变色，测定乳酸生成量的工序。

8. 发明公开

KR1020120099315A 3-하이드록시프로피온산 및 1,3-프로판디올 동시 생산용 재조합미생물 无效
标题翻译：同时生产3-羟基丙酸和1,3-丙二醇的重组微生物
公开(公告)号：KR1020120099315A
公开(公告)日：2012-09-10
申请号：KR1020110007877
申请日：2011-01-26
申请人： 삼성전자주식회사
发明人： 박성민 , 유병조 , 김재영 , 박영경 , 박재찬 , 조화영
IPC分类号： C12N1/21 , C12N15/53 , C12N15/70 , C12P7/18
CPC分类号： C12Y402/0103 , C12N9/0006 , C12N9/0008 , C12N9/88 , C12P7/18 , C12P7/42 , C12Y101/01202 , C12Y102/01
摘要： PURPOSE: A method for preparing 3-HP(3-hydroxypropionic acid) and 1,3-PDO(1,3-propandiol) using microorganisms is provided to convert a glycerol substrate into 3-HP and 1,3-PDO. CONSTITUTION: A recombinant microorganism for preparing 3-HP and 1,3-PDO contains genes producing the compounds. The genes are dhaB(glycerol dehydratease), alhH(aldehyde dehydrogenase), and dhaT(1,3-propandiol oxidoreductase). dhaB gene is derived from K. pneumoniae or C. butyricum. alhH gene is derived from E.coli. The recombinant microorganism is E.coli. A method for producing 3-HP and 1,3-PDO comprises a step of culturing the recombinant microorganism in a medium containing a carbon substrate. The carbon substrate is glucose, sucrose, cellulose, or glycerol.
摘要翻译：目的：提供使用微生物制备3-HP（3-羟基丙酸）和1,3-PDO（1,3-丙二醇）的方法，以将甘油底物转化为3-HP和1,3-PDO。构成：用于制备3-HP和1,3-PDO的重组微生物含有产生该化合物的基因。基因是dhaB（甘油脱水酶），alhH（醛脱氢酶）和dhaT（1,3-丙二醇氧化还原酶）。 dhaB基因衍生自肺炎克雷伯杆菌或C.丁酸菌。 alhH基因来自大肠杆菌。重组微生物是大肠杆菌。制备3-HP和1,3-PDO的方法包括在含有碳底物的培养基中培养重组微生物的步骤。碳底物是葡萄糖，蔗糖，纤维素或甘油。

9. 发明授权

KR102097065B1 4-히드록시부티레이트 생산 균주 및 이를 이용한 4-히드록시부티레이트의 혐기적 생산 방법 有权
公开(公告)号：KR102097065B1
公开(公告)日：2020-04-03
申请号：KR1020130100568
申请日：2013-08-23
申请人： 삼성전자주식회사
发明人： 이영민 , 이우용 , 김진우 , 박재찬 , 박진환 , 조화영
IPC分类号： C12N1/21 , C12N15/52 , C12N15/77 , C12P7/42

10. 发明授权

KR101860442B1 3-하이드록시프로피온산의 생산을 위한 유전자 조작 有权
公开(公告)号：KR101860442B1
公开(公告)日：2018-05-24
申请号：KR1020110062312
申请日：2011-06-27
申请人： 삼성전자주식회사
发明人： 김재영 , 박성민 , 박영경 , 유병조 , 박재찬 , 조화영 , 구현민
IPC分类号： C12N15/53 , C12N15/63 , C12P7/52
CPC分类号： C12P7/42 , C12N9/0006 , C12N9/1029 , C12Y101/01001 , C12Y101/01027 , C12Y203/01008
摘要： Malonic Semialdehyde 환원경로를이용하여 3-하이드록시프로피온산(3-HP)을생산하는데 있어서, 락테이트탈수소화효소(lactate dehydrogenase), 포스포트란스아세틸라아제(phosphotransacetylase) 및알코올탈수소화효소(alcohol dehydrogenase)의발현을저해함으로써중간대사산물의유출(metabolite leak)을막아 Malonyl-CoA 풀(pool)을증가시켜 3-HP 생성효율을현저히높일수 있는방법에관한것이다.

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