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1. 发明公开

KR1020100117465A 호스트셀의 알코올 내성을 증대시키는 단리된 폴리뉴클레오티드, 이를 포함하는 벡터, 호스트셀 및 이를 이용한 알코올의 생산방법 无效
标题翻译：分离多元醇增加酒精耐受性，载体和含有它的宿主细胞，以及使用其制造酒精的方法
公开(公告)号：KR1020100117465A
公开(公告)日：2010-11-03
申请号：KR1020090036253
申请日：2009-04-24
申请人： 삼성전자주식회사 , 성균관대학교산학협력단
发明人： 유병조 , 박재찬 , 박성민 , 권대혁 , 홍민의
IPC分类号： C12N15/31 , C12N15/63 , C12P7/04 , C12R1/865
CPC分类号： C07K14/395
摘要： PURPOSE: A method for producing bio alcohol using a polynucleotide which enhances alcohol resistance of host cells is provided to enhance productivity in alcohol fermentation. CONSTITUTION: An isolated polynucleotide comprises: a polynucleotide comprising nucleotide having 90% or more identity with a nucleotide selected from sequence numbers 1-8; a polynucleotide encoding a polypeptide comprising amino acids having 90% or more identity with amino acids selected from sequence numbers 14-20; a polynucleotide comprising nucleotide sequence hybridizing with the selected nucleotide sequence under severe condition; and a polypeptide encoding polypeptide.
摘要翻译：目的：提供使用增强宿主细胞的耐酒精性的多核苷酸生产生物醇的方法，以提高酒精发酵的生产率。构成：分离的多核苷酸包含：包含与选自序列号1-8的核苷酸具有90％或更高同一性的核苷酸的多核苷酸; 编码多肽的多核苷酸，其包含与选自序列号14-20的氨基酸具有90％以上同一性的氨基酸; 包含在恶劣条件下与选择的核苷酸序列杂交的核苷酸序列的多核苷酸; 和编码多肽的多肽。

2. 发明公开

KR1020130124065A 동시 당화 및 발효를 위한 변형 미생물 无效
标题翻译：改良的微生物同时测定和发酵
公开(公告)号：KR1020130124065A
公开(公告)日：2013-11-13
申请号：KR1020120047683
申请日：2012-05-04
申请人： 삼성전자주식회사 , 성균관대학교산학협력단
发明人： 유병조 , 구현민 , 박성민 , 권대혁 , 김재영 , 박재찬
IPC分类号： C12N1/19 , C12N15/55 , C12P7/06 , C12N15/81
CPC分类号： Y02E50/17 , C12N15/815 , C12N9/2402 , C12P7/10 , C12P7/40 , C12P13/04 , C12P19/02 , C12P19/04
摘要： Disclosed is modified microorganisms for simultaneous saccharification and fermentation, an expression vector for preparing the modified microorganisms, and a method for producing chemical materials using the modified microorganisms. According to one side, disclosed is modified Kluyveromyces marxianus which produces chemical materials by simultaneous saccharification and fermentation and contains a replication origin; a promoter; a gene encodes one or more cellulose decomposing enzymes selected among beta-glucosidase, endoglucanase, exoglucanase, and cellobiohydrolase; and a terminator.
摘要翻译：公开了用于同时糖化和发酵的改性微生物，用于制备改性微生物的表达载体，以及使用改性微生物生产化学物质的方法。一方面，公开了通过同时进行糖化和发酵生产化学物质的马克斯克鲁维酵母（Kluyveromyces marxianus），并含有复制起点; 启动子一种基因编码一种或多种选自β-葡糖苷酶，内切葡聚糖酶，外切葡聚糖酶和纤维二糖水解酶的纤维素分解酶; 和终结者。

3. 发明公开

KR1020100047407A 갈락토오스의 대사 이용율을 증대시키는 유전자, 이를 포함하는 재조합 벡터 및 재조합 미생물 有权
标题翻译：基因增加了糖醇，重组载体和含有其的重组微生物的代谢可用性
公开(公告)号：KR1020100047407A
公开(公告)日：2010-05-10
申请号：KR1020080106278
申请日：2008-10-29
申请人： 삼성전자주식회사 , 성균관대학교산학협력단
发明人： 유병조 , 박재찬 , 구현민 , 진용수 , 이기성
IPC分类号： C12N15/52 , C12N15/56 , C12N15/09
CPC分类号： C12N15/1034 , C07K14/395 , C12P7/04 , C12P7/06 , C12P7/16 , C12P7/28 , Y02E50/10 , Y02E50/17 , C12N15/52 , C12N9/24 , C12N15/09
摘要： PURPOSE: An SNR84 gene enhancing usability of galactose metabolism is provided to enhance metabolic rate of galactose and enhance productivity of bioalcohol from carbon source. CONSTITUTION: An SNR84 gene enhances galactose metabolism rate. The gene is denoted by sequence number 1. A pRS424 recombinant vector contains the SNR84 gene of sequence number 1. A transformed recombinant microorganism is obtained using the recombinant vector. The recombinant microorganism overexpresses the SNR84 gene. The microorganism is yeast. The yeast is Saccharomyces sp., Pachysolen sp., Clavispora sp., Kluyveromyces sp., Debaryomyces sp., Schwanniomyces sp., Candida sp., Pichia sp., or Dekkera sp. The deposit number KCTC 11388 BP of the recombinant microorganism is CEN.PK2-1D/pRS424-SNR84.
摘要翻译：目的：提供增强半乳糖代谢可用性的SNR84基因，以提高半乳糖的代谢率，并提高生物醇从碳源的生产力。构成：SNR84基因增强半乳糖代谢率。该基因由序列号1表示.PRS424重组载体含有序列号1的SNR84基因。使用重组载体获得转化的重组微生物。重组微生物过表达SNR84基因。微生物是酵母。酵母是酵母属（Saccharomyces sp。），Pachysolen sp。，Clavispora sp。，Kluyveromyces sp。，Debaryomyces sp。，Schwanniomyces sp。，Candida sp。，Pichia sp。或Dekkera sp。重组微生物的保藏号KCTC 11388 BP为CEN.PK2-1D / pRS424-SNR84。

4. 发明授权

KR101569210B1 갈락토오스의 대사 이용율을 증대시키는 유전자, 이를 포함하는 재조합 벡터 및 재조합 미생물 有权
标题翻译：基因增加半乳糖重组载体和含有其的重组微生物的代谢可用性
公开(公告)号：KR101569210B1
公开(公告)日：2015-11-23
申请号：KR1020080106278
申请日：2008-10-29
申请人： 삼성전자주식회사 , 성균관대학교산학협력단
发明人： 유병조 , 박재찬 , 구현민 , 진용수 , 이기성
IPC分类号： C12N15/52 , C12N15/56 , C12N15/09
CPC分类号： C12N15/1034 , C07K14/395 , C12P7/04 , C12P7/06 , C12P7/16 , C12P7/28 , Y02E50/10 , Y02E50/17
摘要： 과발현시갈락토오스의대사이용성을증대시킬수 있는유전자에관한기술을제공한다. 구체적인예에서, 과발현시갈락토오스의대사이용성을증가시키는유전자로서, SNR84 유전자를제공한다. SNR84 유전자는과발현됨으로써갈락토오스의대사율을증가시킬수 있으므로, 갈락토오스를함유하는탄소원으로부터바이오알코올의생산성을크게증가시킬수 있다.

5. 发明公开

KR1020130001121A 젖산의 고효율 생산을 위한 변형 미생물 审中-实审
标题翻译：用于高效生产乳酸的改性微生物
公开(公告)号：KR1020130001121A
公开(公告)日：2013-01-03
申请号：KR1020120061819
申请日：2012-06-08
申请人： 삼성전자주식회사 , 성균관대학교산학협력단
发明人： 정순천 , 구현민 , 김재영 , 김지은 , 김진우 , 박영경 , 이소영 , 조화영 , 권대혁 , 박재찬
IPC分类号： C12N1/19 , C12N1/21 , C12N15/53 , C12P7/56
摘要： PURPOSE: A modified microorganism for producing lactic acids and a method for producing lactic acids are provided to produce lactic acids with high efficiency under an acidic condition. CONSTITUTION: A modified microorganism for producing lactic acids with high efficiency has LDH activity of Pelodiscus sinensis japonicus, Ornithorhynchus anatinus, Tursiops truncates, and Rattus norvegicus. The modified microorganism is yeast or bacteria. The modified microorganism is E.coli or Kluyveromyces marxianus. The modified microorganism produces lactic acids with 12.2% or more of glucose. An expression vector for producing the modified microorganism comprises: a replication origin; a promoter; a polynucleotide; and a terminator. A method for producing lactic acids comprises: a step of culturing the modified microorganism in a medium containing glucose; and a step of collecting lactic acids from the culture.
摘要翻译：目的：提供用于生产乳酸的改性微生物和生产乳酸的方法，以在酸性条件下高效生产乳酸。构成：用于高效生产乳酸的改良微生物具有日本芍药，鸟腥草，Tur ps属，and and属的LDH活性。修饰的微生物是酵母或细菌。修饰的微生物是大肠杆菌或马克斯克鲁维酵母（Kluyveromyces marxianus）。经修饰的微生物产生具有12.2％或更多葡萄糖的乳酸。用于产生修饰微生物的表达载体包括：复制起点; 启动子多核苷酸; 和终结者。制备乳酸的方法包括：在含有葡萄糖的培养基中培养经修饰的微生物的步骤; 以及从培养物中收集乳酸的步骤。

6. 发明公开

KR1020130001103A 젖산의 고효율 생산을 위한 변형 미생물 无效
标题翻译：用于高效生产乳酸的改性微生物
公开(公告)号：KR1020130001103A
公开(公告)日：2013-01-03
申请号：KR1020110139520
申请日：2011-12-21
申请人： 삼성전자주식회사 , 성균관대학교산학협력단
发明人： 정순천 , 구현민 , 김재영 , 김지은 , 김진우 , 박영경 , 이소영 , 조화영 , 권대혁 , 박재찬
IPC分类号： C12N15/63 , C12N1/21 , C12N15/53 , C12P7/56
摘要： PURPOSE: A modified microorganism for producing lactic acids is provided to prepare lactic acids with high efficiency under an acidic condition. CONSTITUTION: A modified microorganism for producing lactic acids has a lactate dehydrogenase(LDH) activity of Pelodiscus sinensis japonicus, Ornithorhynchus anatinus, Tursiops truncates, or Rattus norvegicus. The modified microorganism is Escherichia sp. or Kluyveromyces sp. The modified microorganism produces lactic acids with 34% or more of glucose. An expression vector contains: a replication origin for constructing the modified microroganisms; a promoter; a polynucleotide coding LDH activation of one or more species selected from the group consisting of Pelodiscus sinensis japonicus, Ornithorhynchus anatinus, Tursiops truncates, or Rattus norvegicus; and a terminator. The replication origin is ARS/CEN replication origin.
摘要翻译：目的：提供一种用于生产乳酸的改性微生物，用于在酸性条件下高效制备乳酸。构成：用于生产乳酸的改良微生物具有日本芍药（Ornisorhynchus anatinus，Tursiops truncates）或褐藻（Rattus norvegicus）的乳酸脱氢酶（LDH）活性。修饰的微生物是大肠杆菌属或克鲁维酵母属经修饰的微生物产生具有34％以上葡萄糖的乳酸。表达载体包含：用于构建修饰的微结构的复制起点; 启动子编码一种或多种选自以下的物质的LDH活化的多核苷酸：日本Pe us，鸟，us us us，，，;;;;;;;;;;;;;;;;;; 和终结者。复制起点是ARS / CEN复制来源。

7. 发明公开

KR1020130000883A 클루이베로마이세스 마르시아누스 내에서의 향상된 단백질 생산 无效
标题翻译：在KLUYVEROMYCES MARXIANUS中增强蛋白质生产
公开(公告)号：KR1020130000883A
公开(公告)日：2013-01-03
申请号：KR1020110061677
申请日：2011-06-24
申请人： 삼성전자주식회사 , 성균관대학교산학협력단
发明人： 구현민 , 박성민 , 유병조 , 이기성 , 박재찬 , 권대혁
IPC分类号： C12N15/70 , C12N15/81 , C12N15/67
CPC分类号： C12N15/815 , C12P21/00 , C12P21/02
摘要： PURPOSE: An expression vector which overexpresses target proteins, and a method for producing the target protein are provided to obtain the proteins in K. marxianus. CONSTITUTION: An expression vector contains: a replication origin; a CYC promoter, a TEF promoter, a GPD promoter, or an ADH promoter; and a terminator. The CYC promoter contains a sequence of sequence number 1 or a sequence having 70% or more sequence homology with the sequence of sequence number 1. The TEF promoter has a sequence of sequence number 2 or a sequence with 70% or more sequence homology with the sequence of sequence number 2. GPD promoter contains a sequence of sequence number 3 or a sequence having 70% or more homology with the sequence of sequence number 3. The ADH promoter has a sequence of sequence number 4 or a sequence having 70% or more sequence homology with the sequence of sequence number 4.
摘要翻译：目的：提供过表达靶蛋白的表达载体和产生靶蛋白的方法，以获得马克斯马氏霉属中的蛋白质。构成：表达载体包含：复制起点; CYC启动子，TEF启动子，GPD启动子或ADH启动子; 和终结者。 CYC启动子含有序列号1的序列或与序列号1的序列具有70％以上序列同源性的序列.TEF启动子具有序列号2的序列或具有70％以上序列同源性的序列序列号2的序列.GPD启动子含有序列号3的序列或与序列号3的序列具有70％以上同源性的序列.ADH启动子具有序列号4的序列或70％以上的序列与序列号4的序列同源性。

8. 发明公开

KR1020100048224A 갈락토오스의 대사 이용율을 증대시키는 유전자, 이를 포함하는 재조합 벡터 및 재조합 미생물 无效
标题翻译：基因增加了糖醇，重组载体和含有其的重组微生物的代谢可用性
公开(公告)号：KR1020100048224A
公开(公告)日：2010-05-11
申请号：KR1020080107277
申请日：2008-10-30
申请人： 삼성전자주식회사 , 성균관대학교산학협력단
发明人： 유병조 , 박재찬 , 구현민 , 진용수 , 이기성
IPC分类号： C12N15/12 , C12N1/16 , C12N15/66
CPC分类号： C12P7/08 , C07K14/395 , Y02E50/17
摘要： PURPOSE: A gene which enhances metabolic rate of galactose is provided to enhance productivity of bioalcohol from biomass. CONSTITUTION: A gene which enhances metabolic rate of galactose is formed by losing whole or partial expression suppressing domain in a regulatory gene which suppresses expression of galactose matobolic gene. The regulatory gene is a gene encoding a TUP1 protein. A domain suppressing expression of the gene is a C-terminal repressor domain. The regulatory gene has a polynucleotide sequence of sequence number 1. The TUP1 protein has an amino acid sequence of sequence number 2. A pRS424 recombinant vector contains the of sequence number 1. A transformed recombinant microorganism is prepared using the recombinant vector. The microorganism is yeast. The recombinant microorganism is Saccharomyces cerevisiae CEN.PK2-1D/pRS424-truncated TUP1 of deposit number KCTC 11387 BP.
摘要翻译：目的：提高提高半乳糖代谢率的基因，以提高生物质中生物醇的生产力。构成：通过在抑制半乳糖基质的表达的调节基因中失去全部或部分表达抑制结构域，形成提高半乳糖代谢率的基因。调节基因是编码TUP1蛋白的基因。抑制基因表达的结构域是C末端阻遏酶结构域。调节基因具有序列号1的多核苷酸序列.TUP1蛋白具有序列号2的氨基酸序列.PRS424重组载体含有序列号1.使用重组载体制备转化的重组微生物。微生物是酵母。重组微生物是保藏号为KCTC 11387BP的酿酒酵母CEN.PK2-1D / pRS424-截短的TUP1。

9. 发明授权

KR101882740B1 젖산의 고효율 생산을 위한 변형 미생물 有权
公开(公告)号：KR101882740B1
公开(公告)日：2018-07-27
申请号：KR1020120061819
申请日：2012-06-08
申请人： 삼성전자주식회사 , 성균관대학교산학협력단
发明人： 정순천 , 구현민 , 김재영 , 김지은 , 김진우 , 박영경 , 이소영 , 조화영 , 권대혁 , 박재찬
IPC分类号： C12N15/52 , C12P7/56 , C12N15/81 , C12N15/74
摘要： 젖산의고효율생산을위한변형미생물, 상기변형미생물을제작하기위한발현벡터, 및상기변형미생물을이용한젖산의생산방법이개시된다. 상기변형미생물은산성조건하에서도고효율로젖산을생산할수 있다.

10. 发明公开

KR1020100119018A 리그노셀룰로오스계 바이오매스의 전처리 방법 无效
标题翻译：基于木质素的生物质的预处理方法
公开(公告)号：KR1020100119018A
公开(公告)日：2010-11-09
申请号：KR1020090037915
申请日：2009-04-30
申请人： 삼성전자주식회사 , 성균관대학교산학협력단
发明人： 조화영 , 김진우 , 박재찬 , 박성민 , 심상준 , 한세종
IPC分类号： C08B1/08 , C12P7/04 , C10G3/00 , C12R1/865
CPC分类号： C12P7/10 , C08H8/00 , C12P2201/00 , C13K1/02 , Y02E50/16 , Y02E50/343 , Y02P30/20 , C08B1/08 , C10G3/00 , C12P7/04 , C12R1/865
摘要： PURPOSE: A pretreatment method of a lignocellulose-based biomass is provided to obtain cellulose with high purity and hemicelluloses with the high yield, by extracting lignin first. CONSTITUTION: A pretreatment method of a lignocellulose-based biomass comprises the following steps: adding a solvent dissolving lignin to the lignocellulose-based biomass containing the lignin, hemicelluloses, and cellulose, to extract the lignin from the biomass(S1,S2); and adding an ionic solution to the biomass without the lignin, to extract the hemicelluloses and the cellulose(S3). The solvent dissolving the lignin is an alkaline solvent.
摘要翻译：目的：提供木质纤维素基生物质的预处理方法，首先通过提取木质素，以高产率获得高纯度的纤维素和半纤维素。构成：木质纤维素基生物质的预处理方法包括以下步骤：向含木质素，半纤维素和纤维素的木质纤维素基生物质中加入溶解木质素的溶剂，从生物质提取木质素（S1，S2）; 并将离子溶液加入到没有木质素的生物质中，以提取半纤维素和纤维素（S3）。溶解木质素的溶剂是碱性溶剂。

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