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    • 6. 发明公开
    • 젖산의 고효율 생산을 위한 변형 미생물
    • 用于高效生产乳酸的改性微生物
    • KR1020130001121A
    • 2013-01-03
    • KR1020120061819
    • 2012-06-08
    • 삼성전자주식회사성균관대학교산학협력단
    • 정순천구현민김재영김지은김진우박영경이소영조화영권대혁박재찬
    • C12N1/19C12N1/21C12N15/53C12P7/56
    • PURPOSE: A modified microorganism for producing lactic acids and a method for producing lactic acids are provided to produce lactic acids with high efficiency under an acidic condition. CONSTITUTION: A modified microorganism for producing lactic acids with high efficiency has LDH activity of Pelodiscus sinensis japonicus, Ornithorhynchus anatinus, Tursiops truncates, and Rattus norvegicus. The modified microorganism is yeast or bacteria. The modified microorganism is E.coli or Kluyveromyces marxianus. The modified microorganism produces lactic acids with 12.2% or more of glucose. An expression vector for producing the modified microorganism comprises: a replication origin; a promoter; a polynucleotide; and a terminator. A method for producing lactic acids comprises: a step of culturing the modified microorganism in a medium containing glucose; and a step of collecting lactic acids from the culture.
    • 目的:提供用于生产乳酸的改性微生物和生产乳酸的方法,以在酸性条件下高效生产乳酸。 构成:用于高效生产乳酸的改良微生物具有日本芍药,鸟腥草,Tur ps属,and and属的LDH活性。 修饰的微生物是酵母或细菌。 修饰的微生物是大肠杆菌或马克斯克鲁维酵母(Kluyveromyces marxianus)。 经修饰的微生物产生具有12.2%或更多葡萄糖的乳酸。 用于产生修饰微生物的表达载体包括:复制起点; 启动子 多核苷酸; 和终结者。 制备乳酸的方法包括:在含有葡萄糖的培养基中培养经修饰的微生物的步骤; 以及从培养物中收集乳酸的步骤。
    • 7. 发明公开
    • 젖산의 고효율 생산을 위한 변형 미생물
    • 用于高效生产乳酸的改性微生物
    • KR1020130001103A
    • 2013-01-03
    • KR1020110139520
    • 2011-12-21
    • 삼성전자주식회사성균관대학교산학협력단
    • 정순천구현민김재영김지은김진우박영경이소영조화영권대혁박재찬
    • C12N15/63C12N1/21C12N15/53C12P7/56
    • PURPOSE: A modified microorganism for producing lactic acids is provided to prepare lactic acids with high efficiency under an acidic condition. CONSTITUTION: A modified microorganism for producing lactic acids has a lactate dehydrogenase(LDH) activity of Pelodiscus sinensis japonicus, Ornithorhynchus anatinus, Tursiops truncates, or Rattus norvegicus. The modified microorganism is Escherichia sp. or Kluyveromyces sp. The modified microorganism produces lactic acids with 34% or more of glucose. An expression vector contains: a replication origin for constructing the modified microroganisms; a promoter; a polynucleotide coding LDH activation of one or more species selected from the group consisting of Pelodiscus sinensis japonicus, Ornithorhynchus anatinus, Tursiops truncates, or Rattus norvegicus; and a terminator. The replication origin is ARS/CEN replication origin.
    • 目的:提供一种用于生产乳酸的改性微生物,用于在酸性条件下高效制备乳酸。 构成:用于生产乳酸的改良微生物具有日本芍药(Ornisorhynchus anatinus,Tursiops truncates)或褐藻(Rattus norvegicus)的乳酸脱氢酶(LDH)活性。 修饰的微生物是大肠杆菌属 或克鲁维酵母属 经修饰的微生物产生具有34%以上葡萄糖的乳酸。 表达载体包含:用于构建修饰的微结构的复制起点; 启动子 编码一种或多种选自以下的物质的LDH活化的多核苷酸:日本Pe us,鸟,us us us,,,;;;;;;;;;;;;;;;;;; 和终结者。 复制起点是ARS / CEN复制来源。
    • 8. 发明公开
    • 클루이베로마이세스 마르시아누스 내에서의 향상된 단백질 생산
    • 在KLUYVEROMYCES MARXIANUS中增强蛋白质生产
    • KR1020130000883A
    • 2013-01-03
    • KR1020110061677
    • 2011-06-24
    • 삼성전자주식회사성균관대학교산학협력단
    • 구현민박성민유병조이기성박재찬권대혁
    • C12N15/70C12N15/81C12N15/67
    • C12N15/815C12P21/00C12P21/02
    • PURPOSE: An expression vector which overexpresses target proteins, and a method for producing the target protein are provided to obtain the proteins in K. marxianus. CONSTITUTION: An expression vector contains: a replication origin; a CYC promoter, a TEF promoter, a GPD promoter, or an ADH promoter; and a terminator. The CYC promoter contains a sequence of sequence number 1 or a sequence having 70% or more sequence homology with the sequence of sequence number 1. The TEF promoter has a sequence of sequence number 2 or a sequence with 70% or more sequence homology with the sequence of sequence number 2. GPD promoter contains a sequence of sequence number 3 or a sequence having 70% or more homology with the sequence of sequence number 3. The ADH promoter has a sequence of sequence number 4 or a sequence having 70% or more sequence homology with the sequence of sequence number 4.
    • 目的:提供过表达靶蛋白的表达载体和产生靶蛋白的方法,以获得马克斯马氏霉属中的蛋白质。 构成:表达载体包含:复制起点; CYC启动子,TEF启动子,GPD启动子或ADH启动子; 和终结者。 CYC启动子含有序列号1的序列或与序列号1的序列具有70%以上序列同源性的序列.TEF启动子具有序列号2的序列或具有70%以上序列同源性的序列 序列号2的序列.GPD启动子含有序列号3的序列或与序列号3的序列具有70%以上同源性的序列.ADH启动子具有序列号4的序列或70%以上的序列 与序列号4的序列同源性。