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    • 4. 发明公开
    • 아그로박테리움(Agrobacterium)을 이용한사과 '후지' 품종의 형질전환
    • 使用农杆菌遗传转化“富士”苹果
    • KR1020050028329A
    • 2005-03-23
    • KR1020030064437
    • 2003-09-17
    • 대한민국(농촌진흥청장)
    • 김정희송관정황정환신용억
    • A01H1/00
    • C12N15/8205C12N5/0025C12N15/8209C12N15/825C12N2500/34C12N2501/30
    • A method for genetic transformation of 'Fuji' apple using Agrobacterium tumefaciens is provided, thereby improving color development in apples, and reducing labor and costs required for color development of apples, so that production efficiency of apples can be improved. The method for genetic transformation of 'Fuji' apple using Agrobacterium tumefaciens comprises the steps of: (1) culturing apple shoots and rooting the cultured apple shoots in a rooting medium; (2) culturing Agrobacterium tumefaciens containing a foreign gene; (3) inoculating the cultured Agrobacterium tumefaciens to the young leaf cuttings of the apple shoots; (4) culturing the Agrobacterium tumefaciens inoculated young leaf cuttings of the apple shoots in a co-culturing medium; and (5) regenerating the cultured young leaf cuttings of the apple shoots in a regeneration selection medium, wherein the foreign gene is DFR or B-peru.
    • 提供了使用根癌农杆菌对“富士”苹果进行遗传转化的方法,从而改善了苹果的发色,降低了苹果发色所需的劳动和成本,从而可以提高苹果的生产效率。 使用根癌农杆菌对“富士”苹果进行遗传转化的方法包括以下步骤:(1)在生根培养基中培养苹果芽,培养苹果芽; (2)培养含有外来基因的根癌土壤杆菌; (3)将培养的根癌土壤杆菌接种到苹果芽的幼叶片中; (4)在共培养培养基中培养根癌土壤杆菌接种苹果芽的幼叶片; 和(5)在再生选择培养基中再生培养的苹果芽幼嫩叶片,其中外源基因是DFR或B-Peru。
    • 9. 发明公开
    • 사과 바이로이드 감염 진단을 위한 프라이머 및 이를이용한 진단방법
    • 用于诊断苹果皮肤病毒感染的准备和使用该方法进行诊断的方法
    • KR1020100010349A
    • 2010-02-01
    • KR1020080071277
    • 2008-07-22
    • 대한민국(농촌진흥청장)
    • 허성김현란김대일황정환강상조신용억
    • C12N15/10C12N15/29C12Q1/68
    • C12Q1/701C12Q2600/112C12Q2600/13
    • PURPOSE: A primer for diagnosing ASSVd(apple scar skin viroid) infection and a diagnosis method using the same are provided to accurately detect infection by ASSVd and improve diagnosis accuracy. CONSTITUTION: A primer for diagnosing ASSVd(apple scar skin viroid) comprises a sequence number 1 or 2. A method of NASBA(nucleic acid sequence based amplification) for diagnosing infection by ASSVd comprises: a first step of annealing a first primer on a sense RNA and synthesizing single-stranded DNA molecule; a second step of reacting RNase to DNA-RNA hybrid molecule to remove RNA; a third step of annealing a second primer on single-stranded DNA molecule and reacting reverse transcriptase to synthesize double stranded DNA; and a step of synthesizing antisense RNA mediating RNA polymerase with single-stranded DNA molecule as an template.
    • 目的:提供用于诊断ASSVd(苹果瘢痕皮肤病毒)感染的引物和使用其的诊断方法,以准确检测ASSVd感染并提高诊断准确性。 构成:用于诊断ASSVd(苹果瘢痕皮肤病毒)的引物包含序列号1或2.一种用于诊断ASSVd感染的NASBA(基于核酸序列的扩增)的方法包括:第一步骤,对第一引物进行退火 RNA和合成单链DNA分子; 使RNA酶与DNA-RNA杂交分子反应以除去RNA的第二步; 将第二引物退火在单链DNA分子上并使反转录酶反应合成双链DNA的第三步骤; 以单链DNA分子为模板合成反义RNA介导RNA聚合酶的步骤。