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    • 55. 发明授权
    • 누에 혈림프 유래 단백질의 대량 정제방법 및 이를 통해 정제된 단백질
    • 使用丝朊蛋白和纯化蛋白质的蛋白质的质量纯化方法
    • KR101346057B1
    • 2013-12-31
    • KR1020120068585
    • 2012-06-26
    • 대한민국(농촌진흥청장)차의과학대학교 산학협력단
    • 이희삼조유영권해용남성희류강선강필돈박광영김미자최용수김호진황정욱
    • C07K1/14C07K14/435C12N1/04
    • The present invention relates to a mass purification method of a silkworm hemolymph-derived protein and a protein purified thereby. The mass purification method according to the present invention comprises a first step of freeze-drying silkworm to prepare a silkworm dried material; a second step of removing silk gland and obtaining the remainder hemolymph; a third step of crushing the hemolymph and adding water thereto to prepare a hemolymph lysate; a fourth step of subjecting the hemolymph lysate to centrifugation to obtain a supernatant; and a fifth step of subjecting the supernatant to column chromatography to isolate a protein of 70kDa. According to the present invention, the silkworm hemolymph is applicable for several uses, such as a cell cryopreservative, a medium supplement for cell culture, and the like. Especially, the ingredients remaining after the isolation of silkworm hemolymph are also reusable, so that the present invention contributes to an increase in the income of sericulture farmers. [Reference numerals] (AA) Silk gland removal;(BB) Grown-up silkworm;(CC) live body weight 3.13g;(DD) Centrifugation;(EE) Quick freezing;(FF) Grown-up silkworm freeze-drying 590 mg;(GG) pulverization/selection;(HH) hemolymph 380 mg;(II) Dissolution (distilled water);(JJ) 63 mg after heat treatment;(KK) 30k 70k separation;(LL) Final retrieval amount
    • 本发明涉及一种蚕血淋巴衍生蛋白的质量纯化方法和由此纯化的蛋白质。 根据本发明的质量净化方法包括:冷冻干燥蚕的第一步骤,制备蚕干物质; 去除丝腺并获得剩余血淋巴的第二步; 破碎血淋巴并向其中加入水以制备血淋巴溶胞产物的第三步骤; 第四步,使血淋巴溶胞产物离心得到上清液; 和将上清液进行柱色谱分离70kDa的蛋白质的第五步骤。 根据本发明,蚕血淋巴可应用于细胞冷冻保存剂,细胞培养用培养基等的多种用途。 特别是在分离蚕血淋淋后残留的成分也是可重复利用的,因此本发明有助于增加蚕食农民的收入。 (参考号)(AA)丝腺去除;(BB)成熟蚕;(CC)活体重3.13g;(DD)离心;(EE)快速冷冻;(FF)成熟蚕冷冻590 (GG)粉碎/选择;(HH)血淋巴380mg;(II)溶解(蒸馏水);(JJ)热处理后63mg;(KK)30k 70k分离;(LL)最终检索量
    • 60. 发明公开
    • 곡물배지를 이용한 양자에너지 발생물질의 제조방법 및 이의 제조방법에 따라 제조된 양자에너지 발생물질
    • 使用颗粒介质的量子能量材料的制造方法和由其制造的量子能量材料
    • KR1020130012775A
    • 2013-02-05
    • KR1020110074160
    • 2011-07-26
    • 이순일
    • 이순일
    • A61N5/00A61N5/10C12N1/04C12M3/00
    • PURPOSE: A method for manufacturing a quantum energy generation material and the quantum energy generation material manufactured thereby are provided to generate and amplify natural quantum energy using soil with five colors. CONSTITUTION: A method for manufacturing a quantum energy generation material comprises a step(S210) for preparing soil with five colors, a step(S220) for preparing a first soil mixed solution by mixing the soil and a first grain culture medium, a step(S230) for preparing soil bacteria by fermenting the first soil mixed solution, a step(S240) for preparing a soil bacteria mixed solution by mixing the soil bacteria with a second grain culture medium, a step(S250) for preparing a soil bacteria culture solution by culturing the soil bacteria mixed solution, and a step(S260) for precipitating and aging the soil bacteria culture solution. [Reference numerals] (AA) Start; (BB) End; (S210) Step of preparing soil with five colors; (S210-1) Step of collecting the soil from a vein; (S210-2) Step of pulverizing the collected soil using a pulverizer and removing heavy metals and harmful components from the soil; (S210-3) Step of sterilizing and drying the soil; (S220) Step of preparing a first mixed solution by mixing the soil and a grain culture medium; (S230) Step of preparing soil bacteria by fermenting the first soil mixed solution; (S240) Step of preparing a soil bacteria mixed solution by mixing the soil bacteria with the grain culture medium; (S250) Step of preparing a soil bacteria culture solution by culturing the soil bacteria mixed solution; (S260) Step of precipitating and aging the soil bacteria culture solution
    • 目的:提供一种用于制造量子能量产生材料的方法和由此制造的量子能量产生材料,以使用五种颜色的土壤生成和放大天然量子能量。 构成:量子能量生成材料的制造方法包括:制备五色土壤的工序(S210),通过混合土壤和第一谷物培养基来制备第一土壤混合溶液的步骤(S220),步骤 S230),用于通过发酵第一土壤混合溶液来制备土壤细菌的步骤(S240),用于通过将土壤细菌与第二颗粒培养基混合来制备土壤细菌混合溶液的步骤(S240),用于制备土壤细菌培养液的步骤(S250) 通过培养土壤细菌混合溶液,并对土壤细菌培养液进行沉淀和老化的步骤(S260)。 (附图标记)(AA)开始; (BB)结束; (S210)五种颜色的土壤准备步骤; (S210-1)从静脉收集土壤的步骤; (S210-2)使用粉碎机粉碎收集的土壤并从土壤中去除重金属和有害成分的步骤; (S210-3)消毒干燥土壤的步骤; (S220)通过混合土壤和谷物培养基来制备第一混合溶液的步骤; (S230)通过发酵第一土壤混合溶液制备土壤细菌的步骤; (S240)通过将土壤细菌与谷物培养基混合来制备土壤细菌混合溶液的步骤; (S250)通过培养土壤细菌混合溶液制备土壤细菌培养液的步骤; (S260)土壤细菌培养液的沉淀和老化步骤