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    • 6. 发明授权
    • 누에 혈림프 유래 단백질의 대량 정제방법 및 이를 통해 정제된 단백질
    • 使用丝朊蛋白和纯化蛋白质的蛋白质的质量纯化方法
    • KR101346057B1
    • 2013-12-31
    • KR1020120068585
    • 2012-06-26
    • 대한민국(농촌진흥청장)차의과학대학교 산학협력단
    • 이희삼조유영권해용남성희류강선강필돈박광영김미자최용수김호진황정욱
    • C07K1/14C07K14/435C12N1/04
    • The present invention relates to a mass purification method of a silkworm hemolymph-derived protein and a protein purified thereby. The mass purification method according to the present invention comprises a first step of freeze-drying silkworm to prepare a silkworm dried material; a second step of removing silk gland and obtaining the remainder hemolymph; a third step of crushing the hemolymph and adding water thereto to prepare a hemolymph lysate; a fourth step of subjecting the hemolymph lysate to centrifugation to obtain a supernatant; and a fifth step of subjecting the supernatant to column chromatography to isolate a protein of 70kDa. According to the present invention, the silkworm hemolymph is applicable for several uses, such as a cell cryopreservative, a medium supplement for cell culture, and the like. Especially, the ingredients remaining after the isolation of silkworm hemolymph are also reusable, so that the present invention contributes to an increase in the income of sericulture farmers. [Reference numerals] (AA) Silk gland removal;(BB) Grown-up silkworm;(CC) live body weight 3.13g;(DD) Centrifugation;(EE) Quick freezing;(FF) Grown-up silkworm freeze-drying 590 mg;(GG) pulverization/selection;(HH) hemolymph 380 mg;(II) Dissolution (distilled water);(JJ) 63 mg after heat treatment;(KK) 30k 70k separation;(LL) Final retrieval amount
    • 本发明涉及一种蚕血淋巴衍生蛋白的质量纯化方法和由此纯化的蛋白质。 根据本发明的质量净化方法包括:冷冻干燥蚕的第一步骤,制备蚕干物质; 去除丝腺并获得剩余血淋巴的第二步; 破碎血淋巴并向其中加入水以制备血淋巴溶胞产物的第三步骤; 第四步,使血淋巴溶胞产物离心得到上清液; 和将上清液进行柱色谱分离70kDa的蛋白质的第五步骤。 根据本发明,蚕血淋巴可应用于细胞冷冻保存剂,细胞培养用培养基等的多种用途。 特别是在分离蚕血淋淋后残留的成分也是可重复利用的,因此本发明有助于增加蚕食农民的收入。 (参考号)(AA)丝腺去除;(BB)成熟蚕;(CC)活体重3.13g;(DD)离心;(EE)快速冷冻;(FF)成熟蚕冷冻590 (GG)粉碎/选择;(HH)血淋巴380mg;(II)溶解(蒸馏水);(JJ)热处理后63mg;(KK)30k 70k分离;(LL)最终检索量
    • 7. 发明公开
    • 누에 실샘 유래 가수분해물의 수득방법
    • 来自丝M兰的水解产物的获得方法
    • KR1020130031194A
    • 2013-03-28
    • KR1020120068600
    • 2012-06-26
    • 대한민국(농촌진흥청장)차의과학대학교 산학협력단
    • 이희삼조유영권해용남성희류강선강필돈박광영김미자최용수김호진황정욱
    • C07K14/435C07K1/12
    • PURPOSE: A method for preparing a silkworm grand-derived hydrolysate is provided to obtain a hydrolysate with excellent cell proliferation effect without a harmful solvent. CONSTITUTION: A method for preparing a silkworm grand-derived hydrolysate comprises: a step of preparing a silkworm; a step of freeze-drying the silkworm and isolating a silk gland; a step of dissolving the silk gland with water and preparing a first lysate; a step of centrifuging the first lysate and collecting supernatant; a step of adding alkali to the supernatant and performing hydrolysate to prepare a second lysate; a step of adding acid to the second lysate and neutralizing to prepare a neutralized solution; a step of desalting the neutralized solution and preparing a hydrolysate; and a step of freeze-drying the hydrolysate. [Reference numerals] (AA) Silkworm; (BB) Freeze-drying and isolating; (CC) Silk gland; (DD) Pulverizing; (EE) Powder; (FF) Dissolving in water; (GG) Centrifuging and isolating supernatant; (HH) Adding alkali; (II) Hydrolyzing; (JJ) Neutralizing; (KK) Desalting; (LL) Freeze-drying; (MM) Hydrolysate
    • 目的:提供一种制备蚕大分子水解物的方法,以获得没有有害溶剂的具有优异细胞增殖作用的水解产物。 构成:一种蚕源水解产物的制备方法,包括:制备蚕的步骤; 冷冻干燥蚕丝并分离丝腺的步骤; 将丝腺溶解在水中并制备第一裂解液的步骤; 离心第一裂解物并收集上清液的步骤; 向上清液中加入碱,并进行水解产物制备第二裂解液的步骤; 将酸添加到第二裂解物中并中和以制备中和溶液的步骤; 将中和溶液脱盐并制备水解产物的步骤; 和冷冻干燥水解物的步骤。 (附图标记)(AA)蚕; (BB)冷冻干燥和隔离; (CC)丝腺; (DD)粉碎; (EE)粉末; (FF)溶于水; (GG)离心分离上清液; (HH)加碱; (二)水解; (JJ)中和; (KK)脱盐; (LL)冷冻干燥; (MM)水解产物