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    • 3. 发明专利
    • Protein having sweetness
    • 有蛋白质的蛋白质
    • JP2008228690A
    • 2008-10-02
    • JP2007076158
    • 2007-03-23
    • Mitsukan Group Honsha:Kk株式会社ミツカングループ本社
    • ABE KEIKONAKAJIMA KENICHIROASAKURA TOMIKOMITSUSAKA TAKUMIISHII SHO
    • C12N15/09A23L27/00A23L27/30C07K14/415C12N1/15C12N1/19C12N1/21C12N5/10
    • C07K14/415A23L27/31C07K14/43
    • PROBLEM TO BE SOLVED: To find a substance having sweetness without depending on pH and to provide a new composition having sweetness, containing the substance. SOLUTION: The NAS (neoculin acidic subunit) variant is characterized by having sweetness without depending on pH in the case of combination with a polypeptide NBS (neoculin basic subunit)variant by having a variation in substitution or deletion of one or a plurality of amino acids among amino acid residues constituting a polypeptide NAS. The NBS variant is characterized by having sweetness without depending on pH in the case of combination with a polypeptide NAS variant by having a variation in substitution or deletion of one or a plurality of amino acids among amino acid residues constituting a polypeptide NBS. The dimer protein is obtained by combining the NAS variant with the polypeptide NBS variant. The recombinant vector contains a gene encoding the NAS variant or the NBS variant. The transformant contains the vector. COPYRIGHT: (C)2009,JPO&INPIT
    • 要解决的问题:找到不依赖于pH的甜味物质,并提供含有该物质的具有甜味的新组合物。 解决方案:NAS(新兴蛋白酸性亚基)变体的特征在于通过在一个或多个取代或缺失的变化中,与多肽NBS(新霉素基因亚基)变体组合的情况下,不依赖于pH而具有甜度 构成多肽NAS的氨基酸残基之间的氨基酸。 NBS变体的特征在于,通过在构成多肽NBS的氨基酸残基中具有一个或多个氨基酸的取代或缺失的变化,在与多肽NAS变体组合的情况下,不依赖于pH而具有甜味。 通过将NAS变体与多肽NBS变体组合来获得二聚体蛋白。 重组载体含有编码NAS变体或NBS变体的基因。 转化子包含载体。 版权所有(C)2009,JPO&INPIT