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1. 发明专利

JP2008228690A Protein having sweetness 审中-公开
标题翻译：有蛋白质的蛋白质
公开(公告)号：JP2008228690A
公开(公告)日：2008-10-02
申请号：JP2007076158
申请日：2007-03-23
申请人： Mitsukan Group Honsha:Kk , 株式会社ミツカングループ本社
发明人： ABE KEIKO , NAKAJIMA KENICHIRO , ASAKURA TOMIKO , MITSUSAKA TAKUMI , ISHII SHO
IPC分类号： C12N15/09 , A23L27/00 , A23L27/30 , C07K14/415 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10
CPC分类号： C07K14/415 , A23L27/31 , C07K14/43
摘要： PROBLEM TO BE SOLVED: To find a substance having sweetness without depending on pH and to provide a new composition having sweetness, containing the substance. SOLUTION: The NAS (neoculin acidic subunit) variant is characterized by having sweetness without depending on pH in the case of combination with a polypeptide NBS (neoculin basic subunit)variant by having a variation in substitution or deletion of one or a plurality of amino acids among amino acid residues constituting a polypeptide NAS. The NBS variant is characterized by having sweetness without depending on pH in the case of combination with a polypeptide NAS variant by having a variation in substitution or deletion of one or a plurality of amino acids among amino acid residues constituting a polypeptide NBS. The dimer protein is obtained by combining the NAS variant with the polypeptide NBS variant. The recombinant vector contains a gene encoding the NAS variant or the NBS variant. The transformant contains the vector. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：找到不依赖于pH的甜味物质，并提供含有该物质的具有甜味的新组合物。解决方案：NAS（新兴蛋白酸性亚基）变体的特征在于通过在一个或多个取代或缺失的变化中，与多肽NBS（新霉素基因亚基）变体组合的情况下，不依赖于pH而具有甜度构成多肽NAS的氨基酸残基之间的氨基酸。 NBS变体的特征在于，通过在构成多肽NBS的氨基酸残基中具有一个或多个氨基酸的取代或缺失的变化，在与多肽NAS变体组合的情况下，不依赖于pH而具有甜味。通过将NAS变体与多肽NBS变体组合来获得二聚体蛋白。重组载体含有编码NAS变体或NBS变体的基因。转化子包含载体。版权所有（C）2009，JPO＆INPIT

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