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1. 发明专利

JPS6371197A STANDARD COMPOSITION FOR MEASURING BONDED TYPE SIALIC ACID 失效
公开(公告)号：JPS6371197A
公开(公告)日：1988-03-31
申请号：JP21673586
申请日：1986-09-12
申请人： TOYO BOSEKI , SNOW BRAND MILK PROD CO LTD
发明人： TEJIMA SHINICHI , HAYASHI YUZO , DOSEMARI SHUNICHI , IDEIE SAKANORI
IPC分类号： G01N33/52 , C12Q1/00 , C12Q1/32 , C12Q1/34 , C12Q1/37 , C12Q1/527 , G01N33/66
摘要： PURPOSE:The titled standard composition, containing a milk protein, glycoprotein derived therefrom or enzymic hydrolyzate thereof prepared from a relatively inexpensive and readily available substance as a raw material, having stabile quality and useful for diagnosing inflammations, observing progress of symptoms, etc. CONSTITUTION:beta-Lactoglobulin separated from whey in cow's milk as a milk protein, glycoprotein derived therefrom or enzymic hydrolyzate thereof is dissolved in a tris buffer solution at 8.0pH and trypsin dissolved in deionized water is added and reacted at 35 deg.C for 3hr. The reaction mixture is then centrifuged to give a supernatant, which is then dialyzed with deionized water and freeze dried to afford the aimed standard substance containing bonded type sialic acid. Tris buffer solution (7.0pH), neuraminidase, N-acetylneuraminic acid aldolase and reduced form of nicotinamide adenine dinucleotide (NADH) are added and reacted with the standard substance as a test solution to measure absorbance thereof at 340nm wavelength.

2. 发明专利

JP2002045200A METHOD FOR DETECTING NUCLEIC ACID 失效
公开(公告)号：JP2002045200A
公开(公告)日：2002-02-12
申请号：JP2000235554
申请日：2000-08-03
申请人： TOYO BOSEKI , MATSUMOTO KAZUKO
发明人： NISHIYA YOSHIAKI , KIMURA NAOKI , TEJIMA SHINICHI , MATSUMOTO KAZUKO
IPC分类号： G01N33/50 , C12N15/09 , C12Q1/68 , G01N33/58
摘要： PROBLEM TO BE SOLVED: To provide a novel nucleic acid fluorescence assay and provide a method for analyzing gene polymorphism which is useful in the genetic screening that is required for a gene diagnosis and a medical treatment. SOLUTION: This novel nucleic acid fluorescence assay characteristically comprises the step where the oligonucleotides marked with a fluorescent substance represented by the following formula: Ln-S-dNTP (wherein Ln is a ligand of Lanthanide metal or a complex in which a Lanthanide metal is coordinated to the ligand, S is a spacer, dNTP is deoxyribonucleotide derivative) are individually hybridized in one or plural areas in the identical or different DNA samples as templates and the DNA chains are extended by the enzyme reactions and the step where the lanthanide metal complex is formed from the ligand and the lanthanide metal in the case that Ln represents a ligand, and the resultant DNA chains are subjected to the fluorescence assay.

3. 发明专利

JP2001247857A FLUORESCENT SUBSTANCE AND FLUORESCENT LABELING PROCESS 失效
公开(公告)号：JP2001247857A
公开(公告)日：2001-09-14
申请号：JP2000061207
申请日：2000-03-06
申请人： TOYO BOSEKI , MATSUMOTO KAZUKO
发明人： NISHIYA YOSHIAKI , KIMURA NAOKI , TEJIMA SHINICHI , SUJI NAOKI , KAMEZAWA MAKOTO , TOTANI TETSUZO , MATSUMOTO KAZUKO
IPC分类号： G01N33/58 , C07F5/00 , C07H19/10 , C07H21/04 , C09K11/06 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To obtain a fluorescent substance which has a high fluorescence intensity and an excellent fluorescence stability and may be used as a reagent for preparing fluorescent labeling probes or a reagent for chromosomoal diagnosis such as CGH(Comparative Genomic Hybridization), or the like. SOLUTION: The fluorescent substance is represented by the formula: Ln-S-N (wherein Ln is 4,4'-bis(1",1",1",2",2",3",3"-heptafluoro-4",6"-hexanedion-6"-yl) chlorosulfo-o-terphenyl or its derivatives; S is a spacer molecule; and N is a nucleotide).

4. 发明专利

JP2000035384A MULTI FUNCTIONAL OBSERVATION PLATE FOR ANALYZING MATERIAL COMPONENT AND MATERIAL COMPONENT ANALYZING APPARATUS AND METHOD USING THE SAME 失效
公开(公告)号：JP2000035384A
公开(公告)日：2000-02-02
申请号：JP20372298
申请日：1998-07-17
申请人： TOYO BOSEKI , A & T KK
发明人： YONEDA KEIZO , KAWAMURA YOSHIHISA , TEJIMA SHINICHI , SAKAKI TORU , YAMAZAKI TOSHIMITSU
IPC分类号： G01N15/02 , G01N1/00 , G01N1/10
摘要： PROBLEM TO BE SOLVED: To provide a multi functional observation plate capable of suppressing the occurrence of a measuring error in the analysis of a material component and capable of achieving the space saving, wt. reduction and cost reduction of a material component analyzer at the time when used in the analyzer and a material component analyzing apparatus and method using the same. SOLUTION: An observation part 2 is provided to the single side of a plate member 1 and one or more recesses 5-9 are provided to a part other than the observation part 2. The observation part is formed by arranging a light transmission sheet member 3 to the pate member 1. The recess 5 is set to a reaction tank reacting a liquid to be inspected with a reagent to form a soln. to be observed and the recess 6 is set to a reagent tank storing a reagent 6a and the recesses 7, 8 are set to washing soln. tanks storing washing solns. 7a, 8a. The recess 9 is set to a waste soln. tank storing the washing solns. after washing.

5. 发明专利

JP2000019171A ANALYSIS METHOD BY LABELED PROBE 失效
公开(公告)号：JP2000019171A
公开(公告)日：2000-01-21
申请号：JP18485298
申请日：1998-06-30
申请人： MATSUMOTO KAZUKO , TOYO BOSEKI
发明人： MATSUI KAZUHIRO , IKEDA KATSUNORI , TEJIMA SHINICHI , KAWAMURA YOSHIHISA , MATSUMOTO KAZUKO
IPC分类号： G01N33/50 , C12N15/09 , C12Q1/68 , G01N33/533 , G01N33/58
摘要： PROBLEM TO BE SOLVED: To make an object substance measurable with high sensitivity by bonding a labeled body expressed by a specific formula, to a probe of nucleic acid or the like through a cross-linking group or the like so as to react with the object substance on an organismic sample, then adding a heavy metal to obtain a complex, and measuring the fluorescence of the complex. SOLUTION: The labeled body is expressed by formula I or formula II, in which (A1-A3 are each an aromatic group; R1-R3 are each : hydrogen atom or -COCH2COCnF2n+1; (n) is an integer of 1-6). This labeled body is bonded to a probe through a cross-linking group, or a cross-linking group and a linking group to obtain a labeled probe. The probe is selected from a group comprising nucleic acid, nucleic acid bonded protein, low molecular weight ligand and ligand receptor. The object substance is an analysis object component in an organismic sample, and nucleic acid, nucleic acid bonded protein, ligand or ligand receptor is preferable. The organismic sample is dipped in a buffer solution containing this labeled probe and incubated to react the labeled probe with the object substance, and further dipped in a buffer solution containing lanthanoid metal ion after washing, and the fluorescence of a produced fluorescent complex is measured.

6. 发明专利

JP2990753B2 失效
公开(公告)号：JP2990753B2
公开(公告)日：1999-12-13
申请号：JP19428290
申请日：1990-07-23
申请人： TOYO BOSEKI KK
发明人： MIZUGUCHI KATSUHIKO , TEJIMA SHINICHI , HANYU TSUNEO
IPC分类号： C12Q1/34 , C12Q1/40

7. 发明专利

JP2775847B2 失效
公开(公告)号：JP2775847B2
公开(公告)日：1998-07-16
申请号：JP11920089
申请日：1989-05-12
申请人： TOYO BOSEKI KK
发明人： ASANO SHIGEKI , TEJIMA SHINICHI
IPC分类号： G01N33/50 , G01N33/66 , G01N33/68

8. 发明专利

JPH08242860A MODIFIED ENZYME HAVING CHOLESTEROL OXIDASE ACTIVITY 失效
公开(公告)号：JPH08242860A
公开(公告)日：1996-09-24
申请号：JP4733995
申请日：1995-03-07
申请人： TOYO BOSEKI
发明人： NISHIYA YOSHIAKI , HARADA NAOKO , YAMAMOTO KAZUMI , TEJIMA SHINICHI , AISUI SHIGENORI
IPC分类号： C12N15/09 , C12N1/21 , C12N9/04 , C12Q1/26 , C12Q1/28 , C12Q1/44 , C12Q1/60 , C12R1/19 , C12R1/465
摘要： PURPOSE: To obtain a modified enzyme-coding gene, having high stability, enabling the practice of measurement of cholesterol activities with high reliability, useful as a reagent, etc., for clinical tests and improved in thermostability and useful for creating the subject enzyme according to the genetic engineering. CONSTITUTION: This modified enzyme-coding gene codes for an amino acid sequence in which at least one amino acid residue of an amino acid sequence ranging from the amino terminal to an active site is substituted, inserted or deficient in a primary structure of a precursor protein of a cholesterol oxidase (e.g. the primary structure having an amino acid sequence of the formula in the precursor protein of the cholesterol oxidase produced by Streptomyces sp. ASA-COO). A gene, etc., coding for the amino acid sequence in which Ser residue at the 103rd position is substituted with Thr residue are preferred. This creation of the modified enzyme can be achieved according to a genetic engineering technique such as transduction of a site-specific variation into the amino acid sequence of the formula.

9. 发明专利

JPH08187078A NEW ALKALI PHOSPHATASE AND PRODUCTION THEREOF 失效
公开(公告)号：JPH08187078A
公开(公告)日：1996-07-23
申请号：JP335495
申请日：1995-01-12
申请人： TOYO BOSEKI
发明人： HATTORI SHIZUO , YAMAMOTO KAZUMI , TEJIMA SHINICHI
IPC分类号： C12N9/16 , C12R1/645
摘要： PURPOSE: To obtain a new alkali phosphatase useful as a labeling enzyme for biding assay, excellent in thermal stability, and having sugar chain. CONSTITUTION: This alkali phosphatase is obtained by culturing a strain belonging to Acremonium and having ability to produce alkali phosphatase, e.g. Acremonium strictum TE5084 (FERM P-14682) and has the following physicochemical properties: (1) catalyzing a reaction to form an alcohol and orthophosphoric acid from an orthophosphoric monoester and water; (2) acting on p-nitrophenylphosphoric acid, β-glycerophosphoric acid, 4-methyl umbelliferylphosphoric acid, and NADP; (3) optimal temperature: >=50 deg.C, thermal stability at pH7.5 for 15min: =11, stable pH at 25 deg.C for 16h: 6-11; (5) Km value for p-nitrophenylphosphoric acid: 0.41mM; and (6) molecular weight: 630000 (gel filtration method).

10. 发明专利

JPH07322898A REAGENT COMPOSITION FOR MEASURING AMMONIA 失效
公开(公告)号：JPH07322898A
公开(公告)日：1995-12-12
申请号：JP16755595
申请日：1995-07-03
申请人： TOYO BOSEKI
发明人： HONGO TOKUYUKI , HATTORI SHIZUO , TEJIMA SHINICHI , KAWAMURA YOSHIHISA
IPC分类号： C12N9/06 , C12N15/09 , C12Q1/32 , C12Q1/34 , C12Q1/58 , C12R1/38
摘要： PURPOSE:To obtain the subject composition not activated with ADP, containing NADH-dependent glutamic acid dehydrogenase, alpha-ketoglutaric acid (salt), and NADH, not requiring expensive NAD, and good in stability. CONSTITUTION:This ammonia measurement reagent composition not requiring expensive NAD having been necessary as an activating agent, excellent in stability and useful for measuring ammonia, urea form nitrogen, creatinine, etc., is obtained by combining (A) glutamic acid dehydrogenase not activated with ADP, dependent on NADH, stable at approximately 60 deg.C (pH8.3, for 10min), having the optimal pH of 10.5-11.5 (oxidative deamination reaction), stable at a pH of 5-10 (at 25 deg.C for 20hr), having the optimal action temperature of approximately 60 deg.C (oxidative deamination reaction), a specific activity of >=300V/mg, and mol.wts. of approximately 280000 (gel filtration method) and approximately 41000 (SDS-PAGE), (B) alpha-ketoglutaric acid or its salt, and (C) NADH.

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