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1. 发明专利

JP2003194824A FLUORESCENT MATERIAL AND FLUORESCENT LABELING METHOD 审中-公开
公开(公告)号：JP2003194824A
公开(公告)日：2003-07-09
申请号：JP2001394480
申请日：2001-12-26
申请人： TOYO BOSEKI , MATSUMOTO KAZUKO
发明人： TSUJI KATSUMI , NISHIYA YOSHIAKI , TEJIMA SHINICHI , MATSUMOTO KAZUKO
IPC分类号： G01N33/58 , C07H19/10 , C09K11/06 , C12Q1/68 , G01N21/78
摘要： PROBLEM TO BE SOLVED: To provide a fluorescent material usable as a fluorescent labeling probe preparing reagent having high fluorescent intensity, and superior fluorescent stability, and a chromosome diagnosing reagent such as CGH (Comparative Genomic Hybridization). SOLUTION: This fluorescent material is expressed by a general formula (1) Ln-S-N (in the equation, Ln represents N,N,N 1 ,N 1 -[2,6-bis(3'-amino methyl-1'- pyrazolyl)-4-phenyl pyridine] tetrakis (an acetic acid) or its derivative, S represents a spacer molecule, and N represents nucleotide, respectively). COPYRIGHT: (C)2003,JPO

2. 发明专利

JP2002045200A METHOD FOR DETECTING NUCLEIC ACID 失效
公开(公告)号：JP2002045200A
公开(公告)日：2002-02-12
申请号：JP2000235554
申请日：2000-08-03
申请人： TOYO BOSEKI , MATSUMOTO KAZUKO
发明人： NISHIYA YOSHIAKI , KIMURA NAOKI , TEJIMA SHINICHI , MATSUMOTO KAZUKO
IPC分类号： G01N33/50 , C12N15/09 , C12Q1/68 , G01N33/58
摘要： PROBLEM TO BE SOLVED: To provide a novel nucleic acid fluorescence assay and provide a method for analyzing gene polymorphism which is useful in the genetic screening that is required for a gene diagnosis and a medical treatment. SOLUTION: This novel nucleic acid fluorescence assay characteristically comprises the step where the oligonucleotides marked with a fluorescent substance represented by the following formula: Ln-S-dNTP (wherein Ln is a ligand of Lanthanide metal or a complex in which a Lanthanide metal is coordinated to the ligand, S is a spacer, dNTP is deoxyribonucleotide derivative) are individually hybridized in one or plural areas in the identical or different DNA samples as templates and the DNA chains are extended by the enzyme reactions and the step where the lanthanide metal complex is formed from the ligand and the lanthanide metal in the case that Ln represents a ligand, and the resultant DNA chains are subjected to the fluorescence assay.

3. 发明专利

JP2001247857A FLUORESCENT SUBSTANCE AND FLUORESCENT LABELING PROCESS 失效
公开(公告)号：JP2001247857A
公开(公告)日：2001-09-14
申请号：JP2000061207
申请日：2000-03-06
申请人： TOYO BOSEKI , MATSUMOTO KAZUKO
发明人： NISHIYA YOSHIAKI , KIMURA NAOKI , TEJIMA SHINICHI , SUJI NAOKI , KAMEZAWA MAKOTO , TOTANI TETSUZO , MATSUMOTO KAZUKO
IPC分类号： G01N33/58 , C07F5/00 , C07H19/10 , C07H21/04 , C09K11/06 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To obtain a fluorescent substance which has a high fluorescence intensity and an excellent fluorescence stability and may be used as a reagent for preparing fluorescent labeling probes or a reagent for chromosomoal diagnosis such as CGH(Comparative Genomic Hybridization), or the like. SOLUTION: The fluorescent substance is represented by the formula: Ln-S-N (wherein Ln is 4,4'-bis(1",1",1",2",2",3",3"-heptafluoro-4",6"-hexanedion-6"-yl) chlorosulfo-o-terphenyl or its derivatives; S is a spacer molecule; and N is a nucleotide).

4. 发明专利

JP2000019171A ANALYSIS METHOD BY LABELED PROBE 失效
公开(公告)号：JP2000019171A
公开(公告)日：2000-01-21
申请号：JP18485298
申请日：1998-06-30
申请人： MATSUMOTO KAZUKO , TOYO BOSEKI
发明人： MATSUI KAZUHIRO , IKEDA KATSUNORI , TEJIMA SHINICHI , KAWAMURA YOSHIHISA , MATSUMOTO KAZUKO
IPC分类号： G01N33/50 , C12N15/09 , C12Q1/68 , G01N33/533 , G01N33/58
摘要： PROBLEM TO BE SOLVED: To make an object substance measurable with high sensitivity by bonding a labeled body expressed by a specific formula, to a probe of nucleic acid or the like through a cross-linking group or the like so as to react with the object substance on an organismic sample, then adding a heavy metal to obtain a complex, and measuring the fluorescence of the complex. SOLUTION: The labeled body is expressed by formula I or formula II, in which (A1-A3 are each an aromatic group; R1-R3 are each : hydrogen atom or -COCH2COCnF2n+1; (n) is an integer of 1-6). This labeled body is bonded to a probe through a cross-linking group, or a cross-linking group and a linking group to obtain a labeled probe. The probe is selected from a group comprising nucleic acid, nucleic acid bonded protein, low molecular weight ligand and ligand receptor. The object substance is an analysis object component in an organismic sample, and nucleic acid, nucleic acid bonded protein, ligand or ligand receptor is preferable. The organismic sample is dipped in a buffer solution containing this labeled probe and incubated to react the labeled probe with the object substance, and further dipped in a buffer solution containing lanthanoid metal ion after washing, and the fluorescence of a produced fluorescent complex is measured.

5. 发明专利

JP2003254958A NEW FLUORESCENCE DETECTION REAGENT AND HIGHLY-SENSITIVE FLUORESCENCE ANALYTICAL METHOD USING THE SAME 审中-公开
公开(公告)号：JP2003254958A
公开(公告)日：2003-09-10
申请号：JP2002061322
申请日：2002-03-07
申请人： MATSUMOTO KAZUKO
发明人： EN HIROTOSHI , IGARASHI ISAO , FUKUI TAKASHI , MATSUMOTO KAZUKO
IPC分类号： G01N31/00 , C09K11/07 , G01N33/532 , G01N33/58
摘要： PROBLEM TO BE SOLVED: To provide a micro-encapsulating method of a fluorescence label agent, and a new enhancing method. SOLUTION: The method for micro-encapsulating the rare earth fluorescence label agent, and the enhancing method of the rare earth fluorescence label agent through coexistence of enhancing ions and a synergistic ligand are provided. COPYRIGHT: (C)2003,JPO

6. 发明专利

JP2000146965A REAGENT AND KIT FOR IMMUNOLOGICAL ANALYSIS AND IMMUNOLOGICAL ANALYZING METHOD 失效
公开(公告)号：JP2000146965A
公开(公告)日：2000-05-26
申请号：JP31617398
申请日：1998-11-06
申请人： IATRON LAB , MATSUMOTO KAZUKO
发明人： HOSHINO NOBUHIRO , MATSUYA TAKESHI , MATSUMOTO KAZUKO
IPC分类号： G01N33/53 , G01N33/531 , G01N33/532
摘要： PROBLEM TO BE SOLVED: To provide both a reagent for immunological analysis capable of stably maintaining reactivity while developing the advantage of an avidin-biotin reaction and an immunological analyzing method capable of performing speedy, simple, and accurate analysis on compounds to be analyzed. SOLUTION: A reagent for immunological analysis contains a complex of a biotin introducing antibody fragment Fab' or a biotin introducing antigen (1) and a labeled cross-linked avidin (2) subjected to a cross-linking treatment and combined with a labeled substance. The biotin introducing antibody fragment Fab' or the biotin introducing antigen (1) and a post labeled cross-linked avidin (2) combined with a labeled substance after a cross-linking treatment are used for an immunological analyzing method.

7. 发明专利

JPH06107674A CATALYST FOR OXIDATION OR HYDROGENATION OF OLEFIN AND PLATINUM COMPLEX TO BE USED AS THE LIKE 失效
公开(公告)号：JPH06107674A
公开(公告)日：1994-04-19
申请号：JP28537692
申请日：1992-09-30
申请人： MATSUMOTO KAZUKO
发明人： MATSUMOTO KAZUKO
IPC分类号： B01J31/22 , C07B61/00 , C07C27/12 , C07C31/02 , C07C45/29 , C07C45/34 , C07C47/06 , C07C47/22 , C07C49/04 , C07C49/395 , C07C49/433 , C07C49/603 , C07F15/00
摘要： PURPOSE:To obtain an olefin oxide or hydrogenated product of olefin by reaction of an olefin with oxygen or hydrogen using a catalyst under mild conditions. CONSTITUTION:The objective olefin oxide or hydrogenated product can be obtained using a catalyst, a platinum complex of formula (1), (2), (3), or (4) each described below, or a salt thereof; [Pt4(NH3)8(L)4] ...(1); [Pt8(NH3)16(L)8] ...(2); [Pt2(NH3)4(L)2] ...(3); and [Pt2(NH3)4(L)2(L')(L'')] ...(4). In these formulas, L is proton-eliminated amide ligand; L' and L'' are each ligand; n is 5 or 6; m is 9 or 10; 1 is 0, 2, 3 or 4. Of the platinum complexes of the respective formulas (1) to (4) and the salts thereof, those where L is C5H10NO or C7H6NO are new compounds.

8. 发明专利

JP2001199994A LABELING AGENT AND HIGH-SENSITIVITY ANALYSIS USING THE SAME 失效
公开(公告)号：JP2001199994A
公开(公告)日：2001-07-24
申请号：JP2000006884
申请日：2000-01-14
申请人： MATSUMOTO KAZUKO , JAPAN SCIENCE & TECH CORP
发明人： MATSUMOTO KAZUKO
IPC分类号： C07J1/00 , C09K11/06 , G01N33/533
摘要： PROBLEM TO BE SOLVED: To provide a novel labeling agent that forms a fluorescent complex with Eu3+ ion, A high-sensitive assay that uses the novel labeling agent, particularly for analyzing estrogen in the environment with a high level of sensitivity. SOLUTION: The novel labeling agent according to this invention has a chemical structure represented by formula (I) (wherein n is an integer of from 2 to 5). In the high-sensitivity assay according to this invention, the object is labeled by allowing it to react with the labeling agent, the labeled object is separated by chromatography, the isolated object is allowed to react with the Eu ion to form a Eu ion complex. Then, the concentration of the Eu ion complex is determined by the time-resolved fluorometric detection.

9. 发明专利

JPH09241233A LABELING REAGENT FOR IMMUNOLOGICAL MEASUREMENT AND FLUORESCENT COMPOUND AND COMPLEX USING THE SAME, AND IMMUNOLOICAL MEASUREMENT OF BIOLOGICAL SUBSTANCE WITH THE SAME 失效
公开(公告)号：JPH09241233A
公开(公告)日：1997-09-16
申请号：JP5118596
申请日：1996-03-08
申请人： MATSUMOTO KAZUKO , SUZUKI MOTOR CO
发明人： MATSUMOTO KAZUKO , EN HIROTOSHI
IPC分类号： C07C45/45 , C07C49/782 , C07C49/813 , C07C49/92 , C07C309/86 , C07F5/00 , C09K11/06 , G01N33/533
摘要： PROBLEM TO BE SOLVED: To obtain a fluorescent compound large in fluorescent intensity, capable of being synthesized in a high yield and at a low cost, and useful as a labeling reagent for immunological measurements, capable of being used for both solid phase measurements and liquid phase measurements in immunological measurement methods and rapidly giving measurement results in a few measuring processes. SOLUTION: This complex comprises a compound of formula I [R is a group capable of being bound to proteins; Ar is a group having conjugated double bonds; X is F, a group of formula II; (n) is an integer] and a lanthanide metal ion. The compound of formula I is obtained by subjecting an acetylated aromatic ring compound and an ethyl perfluorocarboxylate compound or a diethyl perfluorodicarboxylate compound to a Claisen's condensation reaction and subsequently subjecting the obtained β-diketone compound and chlorosulfuric acid to a chlorosulfonation reaction. The labeling reagent can directly be bound to a measurement target, a protein such as an antigen or antibody, and can emit fluorescent light in a state of the reagent alone and also in a state bound to the protein.

10. 发明专利

JP2003116581A LABELED NUCLEIC ACID AND METHOD FOR PRODUCING THE SAME 审中-公开
公开(公告)号：JP2003116581A
公开(公告)日：2003-04-22
申请号：JP2001321104
申请日：2001-10-18
申请人： DNA CHIP KENKYUSHO KK , HITACHI SOFTWARE ENG , MATSUMOTO KAZUKO
发明人： KOMATSU YASUO , OTSUKA EIKO , MATSUMOTO KAZUKO
IPC分类号： G01N33/53 , C12M1/00 , C12N15/09 , C12Q1/68 , G01N33/543 , G01N33/566 , G01N33/58
摘要： PROBLEM TO BE SOLVED: To use a β-diketone europium complex for detecting a nucleic acid in a region at a low concentration which cannot be detected with a conventional fluorescent dye in a DNA chip or a DNA microarray and to provide a method for assaying an immobilized DNA immobilized on a stationary phase such as a glass or a membrane. SOLUTION: A labeling substance represented by the general formula (I) is bound through a specific connecting group to the nucleic to provide a labeled nucleic acid.

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