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1. 发明专利

JP2009136201A Method for preserving biological component 审中-公开
标题翻译：保存生物成分的方法
公开(公告)号：JP2009136201A
公开(公告)日：2009-06-25
申请号：JP2007315271
申请日：2007-12-05
申请人： Kazuhiko Ishihara , Sumitomo Bakelite Co Ltd , 住友ベークライト株式会社 , 一彦石原
发明人： KAWAMURA KENJI , ISHIHARA KAZUHIKO , KONNO TOMOHIRO
IPC分类号： C12N5/07 , C12N5/071 , C12N5/10
摘要： PROBLEM TO BE SOLVED: To provide a method for preserving a biological component immobilized without freezing in a medium consisting essentially of water under mild conditions to a biological component of normal temperature and normal pressure.
SOLUTION: The method for preserving a biological component comprises mixing a water-soluble polymer solution into which a biological component is dispersed with a water-soluble compound solution to form a three-dimensional crosslinked product and preserving the biological component in the state of the three-dimensional crosslinked product. Preferably the water-soluble polymer is a polymer containing a phosphorylcholine group and a phenylboronic acid group and the water-soluble compound is a compound containing a polyfunctional hydroxy group.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：待解决的问题：提供一种在温和条件下将基本上由水组成的培养基中保持固定而不冷冻的生物成分与常温常压生物成分相比较的方法。解决方案：保存生物成分的方法包括将其中分散有生物成分的水溶性聚合物溶液与水溶性化合物溶液混合以形成三维交联产物并将该生物成分保持在该状态的三维交联产物。优选地，水溶性聚合物是含有磷酸胆碱基团和苯基硼酸基团的聚合物，并且水溶性化合物是含有多官能羟基的化合物。版权所有（C）2009，JPO＆INPIT

2. 发明专利

JP2010220570A Method for laminating cell 有权
标题翻译：层状细胞的方法
公开(公告)号：JP2010220570A
公开(公告)日：2010-10-07
申请号：JP2009073208
申请日：2009-03-25
申请人： Sumitomo Bakelite Co Ltd , 住友ベークライト株式会社
发明人： KAWAMURA KENJI
IPC分类号： C12N5/07
摘要： PROBLEM TO BE SOLVED: To provide an organism-resembled tissue by constructing a three-dimensional structure comprising cells by laminating the cells.
SOLUTION: The method for laminating the cells includes: forming a water-soluble polymer layer having a cell adhesion peptide as a first substance in the structure, and a water-soluble polymer layer as a second substance on the upper side of a single cell layer formed on the surface of a single base material, and further forming a single cell layer thereon. Preferably, a plurality of the first substance layers and the second substance layers are alternately constituted, and the single cell layer comprises the connective tissue cells or the epidermal cells. Preferably, the method for laminating the cells of laminating a plurality of the layers of the connective tissue cells and/or functional cells includes: repeating the method for laminating the cells for several times.
COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：通过层叠细胞来构建包含细胞的三维结构来提供生物体组织。解决方案：用于层压细胞的方法包括：在结构中形成具有细胞粘附肽作为第一物质的水溶性聚合物层，以及作为第二物质的水溶性聚合物层作为第二物质单细胞层形成在单个基材的表面上，并且在其上进一步形成单细胞层。优选地，交替地构成多个第一物质层和第二物质层，并且单细胞层包含结缔组织细胞或表皮细胞。优选地，层叠多个结缔组织细胞和/或功能细胞层的细胞的方法包括：重复用于层叠细胞数次的方法。版权所有（C）2011，JPO＆INPIT

3. 发明专利

JP2009296909A Method for dissolving three-dimensional cross-linked body 审中-公开
标题翻译：解决三维交叉体的方法
公开(公告)号：JP2009296909A
公开(公告)日：2009-12-24
申请号：JP2008152937
申请日：2008-06-11
申请人： Sumitomo Bakelite Co Ltd , 住友ベークライト株式会社
发明人： KAWAMURA KENJI
IPC分类号： C12N5/10 , C12N5/07 , C12N5/0735 , C12N5/074
摘要： PROBLEM TO BE SOLVED: To provide a method for easily dissolving a three-dimensional cross-linked body after preservation and transportation of the three-dimensional cross-linked body obtained by entrapping and immobilizing a living body component in a hydrogel being a three-dimensional cross-linked body and recovering the living body part of contents.
SOLUTION: This method is a method for dissolving a three-dimensional cross-linked body obtained by immobilizing a living body component in which the three-dimensional cross-linked body is formed by mixing a water-soluble polymer solution with a water-soluble compound solution and is brought into contact with a dissolving solution containing a compound having a plurality of hydroxy groups. Preferably, the water-soluble polymer is a polymer containing a phosphorylcholine group and a phenyl boronic acid group and the water-soluble compound is a polyfunctional hydroxy group-containing compound and the compound containing a plurality of hydroxy groups is a sugar alcohol.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：待解决的问题：提供一种容易溶解三维交联体的方法，该三维交联体在通过将生物体成分捕获并固定在水凝胶中而获得的三维交联体保存和输送后三维交联体，恢复活体内容物。解决方案：该方法是将通过将水溶性聚合物溶液与水混合形成三维交联体的活体成分固定而得到的三维交联体溶解的方法与含有具有多个羟基的化合物的溶解溶液接触。优选地，水溶性聚合物是含有磷酸胆碱基团和苯基硼酸基团的聚合物，并且水溶性化合物是含多官能羟基的化合物，并且含有多个羟基的化合物是糖醇。版权所有（C）2010，JPO＆INPIT

4. 发明专利

JP2004340818A Immunoassay container 审中-公开
公开(公告)号：JP2004340818A
公开(公告)日：2004-12-02
申请号：JP2003139139
申请日：2003-05-16
申请人： Sumitomo Bakelite Co Ltd , 住友ベークライト株式会社
发明人： KAWAMURA KENJI
IPC分类号： G01N33/543
摘要： PROBLEM TO BE SOLVED: To provide an immunoassay container capable of shortening greatly a measuring time, and controlling surely physico-chemical adsorption, in the immunoassay by an ELISA method.
SOLUTION: This immunoassay container has antibody bonding molecules on the surface of a portion used for an immunoreaction on the immunoassay container surface, and the other region thereof is subjected to hydrophilicity treatment. Preferably, the antibody bonding molecule is at least one selected from protein A, protein G, an anti-Fc antibody and an anti-Fc antibody Fab fragment, and the hydrophilicity treatment is application of a hydrophilic polymer, graft polymerization of hydrophilic monomers, or a combination thereof.
COPYRIGHT: (C)2005,JPO&NCIPI

5. 发明专利

JP2004166532A Culture container 审中-公开
标题翻译：文化容器
公开(公告)号：JP2004166532A
公开(公告)日：2004-06-17
申请号：JP2002333954
申请日：2002-11-18
申请人： Sumitomo Bakelite Co Ltd , 住友ベークライト株式会社
发明人： KAWAMURA KENJI , TAKEYAMA KENJIRO
IPC分类号： C12M3/00
摘要： PROBLEM TO BE SOLVED: To provide a culture container whose cover can simply be removed, when needed, and from which cultured cells can easily be recovered.
SOLUTION: This culture container for culturing cells, comprising a main body and a cover, is characterized in that the main body and the cover are fixed with a heat-shrinkable film. In the culture container, a rubber O-ring or a packing is preferably mounted between the main body and the cover.
COPYRIGHT: (C)2004,JPO
摘要翻译：要解决的问题：提供一种培养容器，其能够根据需要简单地除去盖子，并且可以容易地回收培养的细胞。解决方案：用于培养细胞的培养容器，其包括主体和盖子，其特征在于，主体和盖子用热收缩膜固定。在培养容器中，橡胶O形圈或填料优选安装在主体和盖之间。版权所有（C）2004，JPO

6. 发明专利

JP2004361295A Immune analyzing vessel 审中-公开
公开(公告)号：JP2004361295A
公开(公告)日：2004-12-24
申请号：JP2003161422
申请日：2003-06-06
申请人： Sumitomo Bakelite Co Ltd , 住友ベークライト株式会社
发明人： KAWAMURA KENJI
IPC分类号： G01N33/543
摘要： PROBLEM TO BE SOLVED: To provide an immune analyzing vessel for surely fixing a primary antibody in a short time and eliminating blocking operation in an immune analysis using an ELISA method.
SOLUTION: A blocking agent is derived from a protein, blocks specific absorption of an antibody coupling molecule and the protein and is previously applied to a surface of a region used for reaction in the immune analyzing vessel. The antibody coupling molecule is preferably selected from a protein A, a protein G, an anti-Fc antibody and an anti-Fc antibody Fab fragment. The blocking agent is selected from an albumin, a milk protein, a gelatine and a collagen.
COPYRIGHT: (C)2005,JPO&NCIPI

7. 发明专利

JP2004222545A Culture container with cell 审中-公开
公开(公告)号：JP2004222545A
公开(公告)日：2004-08-12
申请号：JP2003011719
申请日：2003-01-21
申请人： Sumitomo Bakelite Co Ltd , 住友ベークライト株式会社
发明人： KAWAMURA KENJI
IPC分类号： C12M3/06
摘要： PROBLEM TO BE SOLVED: To provide a culture container with cells, with which liquids are replaced without damaging cells in a microplate after the cells are frozen and melted in a cultured state, optical microscope observation and fluorescence microscope observation are made.
SOLUTION: The culture container with cells is a culture container in which cells are frozen in a cultured state, has porous structure through which a culture solution is passed so that cells are not floated and removed from a culture surface in replacing culture solutions immediately after melting. The porous structure is detachable from the culture container with cells. Preferably the porous structure has a knob and is readily disconnectable.
COPYRIGHT: (C)2004,JPO&NCIPI

8. 发明专利

JP2004138583A Micro-chip for measuring function of cell 审中-公开
公开(公告)号：JP2004138583A
公开(公告)日：2004-05-13
申请号：JP2002305852
申请日：2002-10-21
申请人： Sumitomo Bakelite Co Ltd , 住友ベークライト株式会社
发明人： SAWAI HIROSHI , YOKOYAMA KANEHISA , KAWAMURA KENJI
IPC分类号： G01N31/20 , C12M1/00 , C12M1/34 , C12Q1/02 , G01N27/447 , G01N30/60 , G01N30/88 , G01N33/53 , G01N37/00
CPC分类号： G01N30/6095
摘要： PROBLEM TO BE SOLVED: To more easily and more accurately identify a protein, unlike the Western blot that requires the time, in viewing an intracellular expression of the protein. SOLUTION: The micro-chip measures the function of a cell, by the protein in the cell. A cell culture part, a cell crush part, a cell residue removal part, a protein solubilization part, a protein refining part, a protein separation part, and a protein identification part are provided, in which each part is successively constituted, using a groove on the chip, a connector, and/or a tube. The operations from the cell culture to the determination of the protein to be detected are successively performed. COPYRIGHT: (C)2004,JPO

9. 发明专利

JP2006087396A Cell culture substrate and method for producing the same 审中-公开
标题翻译：细胞培养基底及其制造方法
公开(公告)号：JP2006087396A
公开(公告)日：2006-04-06
申请号：JP2004279893
申请日：2004-09-27
申请人： Katsumi Mochidate , National Institute For Environmental Studies , Sumitomo Bakelite Co Ltd , 住友ベークライト株式会社 , 克身持立 , 独立行政法人国立環境研究所
发明人： MOCHIDATE KATSUMI , KAWAMURA KENJI , TAKADA WATARU
IPC分类号： C12N5/10 , A61L27/00 , C07K14/475 , C07K14/78 , C12N5/07 , C12N5/071
摘要： PROBLEM TO BE SOLVED: To provide a new cell culture substrate able to solidify two or more peptides on the surface of the substrate in an amount of arbitrarily controlled introduction and controlling adhesion, growth, proliferation, and the like, of cells by combining a plurality of kinds of peptides and provide a method for producing the same. SOLUTION: The method for producing the cell culture substrate has a process for coating a peptide-introduced polymer, in which single kind of peptide is introduced into a hydrophobically bonded adsorbing polymer, onto the surface of the substrate for culturing two or more kinds of cells. Preferably, the hydrophobically bonded adsorbing polymer has, in the molecule, a hydrophobic straight chain carbon skeleton and functional groups able to react with peptides in the molecule. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供一种新的细胞培养基质，其能够以任意控制的量引导和控制细胞的粘附，生长，增殖等，使基底表面上的两种或更多种肽固化组合多种肽并提供其制备方法。解决方案：细胞培养基质的制造方法具有将单体肽引入到疏水键合的吸附性聚合物中的肽导入性聚合物的涂布于基材的表面上进行培养2次以上的方法细胞种类优选地，疏水键合的吸附聚合物在分子中具有疏水性直链碳骨架和能够与分子中的肽反应的官能团。版权所有（C）2006，JPO＆NCIPI

10. 发明专利

JP2003294741A Microchip for cell function measurement 审中-公开
标题翻译：细胞功能测量的MICROCHIP
公开(公告)号：JP2003294741A
公开(公告)日：2003-10-15
申请号：JP2002094067
申请日：2002-03-29
申请人： Sumitomo Bakelite Co Ltd , 住友ベークライト株式会社
发明人： KAWAMURA KENJI
IPC分类号： G01N33/53 , C12M1/34 , C12M3/00 , G01N37/00
摘要： PROBLEM TO BE SOLVED: To provide a container for simply and efficiently extracting a minute substance from cultivated cells and supplying the substance to a specimen.
SOLUTION: This microchip for cell function measurement is characterized in that it comprises a cell cultivation part and a measurement chip part, and cells are cultivated in the cultivation part, and the cultivation part comprises a molded plastic article and can be reserved in a frozen state.
COPYRIGHT: (C)2004,JPO

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