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1. 发明专利

JP2003164286A MANNANASE AND MANNANASE GENE 审中-公开
公开(公告)号：JP2003164286A
公开(公告)日：2003-06-10
申请号：JP2001365829
申请日：2001-11-30
申请人： NODA INST FOR SCIENTIFIC RES , KIKKOMAN CORP
发明人： UMITSUKE GENRYU , HARA SEIICHI , HATAMOTO OSAMU , MASUDA TSUTOMU , KOYAMA TAIJI , MATSUDA TOSHIBUMI , ITO KOTARO , TAKAHASHI OSAMU , MATSUSHIMA KENICHIRO
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/24
摘要： PROBLEM TO BE SOLVED: To provide a new mannanase, a gene encoding the enzyme, a recombinant vector containing the gene and a transformant transformed by the vector and to provide a method for producing the mannanase by using the transformant. SOLUTION: This invention provides a protein containing a specific amino acid sequence, the mannanase gene encoding the protein, the mannanase gene containing DNA containing a specific base sequence, the recombinant vector containing the gene, the transformant containing the recombinant vector and the method for producing the mannanase by collecting the mannanase protein from culture products obtained by culturing the transformant. COPYRIGHT: (C)2003,JPO

2. 发明专利

JP2001046053A MODIFICATION OF GENOME TARGET SITE OF MULTINUCLEATED CELL, TARGETING VECTOR USED THEREFOR AND MODIFIED MULTINUCLEATED CELL 失效
公开(公告)号：JP2001046053A
公开(公告)日：2001-02-20
申请号：JP22891599
申请日：1999-08-12
申请人： KIKKOMAN CORP
发明人： HATAMOTO OSAMU , MATSUSHIMA KENICHIRO , TAKAHASHI OSAMU , UMITSUKE GENRYU , ABE TAKAYOSHI
IPC分类号： C12N15/09 , C12N1/15 , C12N5/10 , C12N15/80
摘要： PROBLEM TO BE SOLVED: To obtain a target vector for modifying genome target site of a multinucleated cell, suitable for creating gene-cleaved strain by including a DNA containing modified target site or the like. SOLUTION: This targeting vector contains (A) a target site of a gene or the like encoding modified enzyme, peptide hormone, enzyme corresponding to a synthetic system of a biologically active substance or enzyme corresponding to a toxin-biosynthesizing system, (B) a recombinant section marker comprising a positive section marker and a negative section marker, and (C) a marker for controlling the copy number encoding an enzyme selected from a group of a laccase, a β-galactosidase, a β-glucuronidase and an alkaline phosphatase. The multinucleated cell is preferably the one derived from an organism belonging to the genus selected from Aspergillus, Penicillium, Neurospora and Fusarium.

3. 发明专利

JP2013230141A Method for producing duplicated translocation of optional region in aspergillus chromosome 有权
标题翻译：用于生产ASPERGILLUS染色体中可选区域的重复转录的方法
公开(公告)号：JP2013230141A
公开(公告)日：2013-11-14
申请号：JP2013052795
申请日：2013-03-15
申请人： Kikkoman Corp , キッコーマン株式会社
发明人： TAKAHASHI OSAMU
IPC分类号： C12N1/15 , A23L27/50 , C12N15/09 , C12R1/66 , C12R1/69
CPC分类号： C12N15/80 , A23L27/50
摘要： PROBLEM TO BE SOLVED: To provide a technique to duplicate and translocate any large region in Aspergillus chromosome across a wide range, so that it will be possible to and stably and systematically acquire an Aspergillus strain having a new trait, which was un-acquirable by conventional techniques.SOLUTION: A fungus belonging to genus Aspergillus wherein a transformation marker gene with deficiency of a terminal part at the 5' end or 3' end of its coding region is integrated into an outside of a target region in a chromosome of the fungus subject to duplicated translocation, and a transformation marker gene with deficiency of a terminal part at the 3' end or 5' end is integrated into an outside of a region in another chromosome of the fungus to be replaced with the target region.
摘要翻译：要解决的问题：提供一种在广泛范围内复制和转运曲霉菌染色体中的任何大区域的技术，使得可以并且稳定和系统地获得具有新特征的曲霉菌株，其不可获得常规技术。解决方案：属于曲霉菌属的真菌，其中在其编码区的5'端或3'端的末端部分缺失的转化标记基因整合到真菌染色体中的靶区域的外侧受复制的易位，并且在3'末端或5'端的末端部分缺失的转化标记基因整合到真菌的另一个染色体中的区域的外部，以被靶区域替代。

4. 发明专利

JP2003250580A PROLIDASE, GENE THEREOF AND METHOD FOR PRODUCING PROLIDASE 审中-公开
公开(公告)号：JP2003250580A
公开(公告)日：2003-09-09
申请号：JP2002283759
申请日：2002-09-27
申请人： KIKKOMAN CORP , NODA INST FOR SCIENTIFIC RES
发明人： ITO KOUTARO , NAKAHARA TAKEHARU , KOYAMA TAIJI , MATSUDA TOSHIBUMI , TAKAHASHI OSAMU , MATSUSHIMA KENICHIRO , UMITSUKE GENRYU , MASUDA TSUTOMU
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/48 , C12N9/62
摘要： PROBLEM TO BE SOLVED: To improve the protein engineering of prolidase and improve microorganisms which are used in the production of enzymes for food processing and in the production of brewed foods. SOLUTION: This invention provides a protein that comprises an amino acid sequence represented by a sequence No.2 (refer to the specification) and the prolidase activity, a prolidase gene that comprises a base sequence represented by a sequence No.1 (refer to the specification), a recombinant DNA formed by transferring the gene into a vector, a transformant or a transductant including the recombinant DNA, and a method for producing the prolidase using the transformant or the transductant. COPYRIGHT: (C)2003,JPO

5. 发明专利

JP2003219888A AMINOPEPTIDASE P, AMINOPEPTIDASE P GENE, RECOMBINANT DNA AND METHOD FOR PRODUCING AMINOPEPTIDASE P 审中-公开
公开(公告)号：JP2003219888A
公开(公告)日：2003-08-05
申请号：JP2002024887
申请日：2002-02-01
申请人： KIKKOMAN CORP , NODA INST FOR SCIENTIFIC RES
发明人： MATSUSHIMA KENICHIRO , KOYAMA TAIJI , TAKAHASHI OSAMU , MATSUDA TOSHIBUMI , ITO KOUTARO , NAKAHARA TAKEHARU , MASUDA TSUTOMU , UMITSUKE GENRYU
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/64
摘要： PROBLEM TO BE SOLVED: To provide a new aminopeptidase P, an aminopeptidase P gene, a recombinant DNA, a method for producing the aminopeptidase P, a method for protein-engineering improvement of the aminopeptidase P and a method for improving microorganisms for the production of enzymes for food processing use or the production of fermented foods. SOLUTION: The invention provides an aminopeptidase P composed of a specific amino acid sequence, an aminopeptidase P gene encoding the protein, an aminopeptidase gene composed of a specific base sequence, a recombinant DNA obtained by inserting the gene into a vector DNA, a transformant or transductant containing the recombinant DNA, and a method for producing the aminopeptidase P by culturing the transformant or transductant in a medium and separating the produced aminopeptidase P from the cultured product. COPYRIGHT: (C)2003,JPO

6. 发明专利

JP2004008106A PROLINE IMINOPEPTIDASE, PROLINE IMINOPEPTIDASE GENE, RECOMBINANT VECTOR AND METHOD FOR PRODUCING PROLINE IMINOPEPTIDASE 审中-公开
公开(公告)号：JP2004008106A
公开(公告)日：2004-01-15
申请号：JP2002166755
申请日：2002-06-07
申请人： KIKKOMAN CORP , NODA INST FOR SCIENTIFIC RES
发明人： TAKAHASHI OSAMU , KOYAMA TAIJI , MATSUSHIMA KENICHIRO , MATSUDA TOSHIBUMI , ITO KOUTARO , NAKAHARA TAKEHARU , UMITSUKE GENRYU , MASUDA TSUTOMU
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/62
摘要： PROBLEM TO BE SOLVED: To provide a new proline iminopeptidase, a proline iminopeptidase gene, a recombinant vector and a method for producing the praline iminopeptidase. SOLUTION: The protein comprises a specific amino acid sequence derived from Aspergillus oryzae, or an amino acid sequence formed by deleting, substituting or inserting one or several amino acids from, with or to the amino acid sequence, and has proline iminopeptidase activities. The proline iminopeptidase gene encodes the protein. COPYRIGHT: (C)2004,JPO

7. 发明专利

JP2013034416A BACTERIUM HAVING OVERLAPPED GENOMIC REGION 审中-公开
公开(公告)号：JP2013034416A
公开(公告)日：2013-02-21
申请号：JP2011171963
申请日：2011-08-05
申请人： KIKKOMAN CORP
发明人： TAKAHASHI OSAMU , SATO ATSUSHI
IPC分类号： C12N15/09 , A23L27/50 , C12N1/15
摘要： PROBLEM TO BE SOLVED: To enable the production of a koji fungus having a new character, which cannot be obtained so far, in a steady and systematic manner on the basis of a technique for overlapping an arbitrary genomic region on a chromosome of a fungus belonging to the genus Aspergillus.SOLUTION: The transformant of the fungus belonging to the genus Aspergillus can produce a wide variety of groups of enzymes necessary for the production of soy source, e.g. proteases, at high levels. The transformant has overlapped genomic regions in tandem on the same chromosome and also has a transformed marker gene integrated into a region sandwiched by the overlapped genomic regions.

8. 发明专利

JP2012187102A Novel protease gene, recombinant dna, and method for making protease 审中-公开
标题翻译：新型蛋白酶基因，重组DNA和制备蛋白质的方法
公开(公告)号：JP2012187102A
公开(公告)日：2012-10-04
申请号：JP2012039230
申请日：2012-02-24
申请人： Kikkoman Corp , Noda Institute For Scientific Research , キッコーマン株式会社 , 公益財団法人野田産業科学研究所
发明人： OGAWA SHINKO , SATO ATSUSHI , TAKAHASHI OSAMU , OSHIMA KENSHIRO , HATTORI SHOHEI , KOYAMA TAIJI , OGUMA TETSUYA , HANYA YOSHISATO
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/62
摘要： PROBLEM TO BE SOLVED: To provide: a novel protease capable of decomposing a protein present in a raw material such as a soy sauce and an enzyme decomposing seasoning; a novel gene encoding the protease; a recombinant DNA including the gene; a transformant or a transductant including the recombinant DNA; and a method for making the protease using the transformant and the transductant.SOLUTION: The method for making the protease is characterized by: culturing the proteins having protease activity including a specific amino acid sequence or others, a gene encoding the protein or others, the recombinant vector including the gene, the transformant including the recombinant vector, and the transformant; and recovering the protease from the obtained cultured substance.
摘要翻译：待解决的问题：提供：能够分解存在于原料如酱油和酶分解调味料中的蛋白质的新型蛋白酶; 编码蛋白酶的新基因; 包含该基因的重组DNA; 包含重组DNA的转化体或转导体; 以及使用转化体和转导体制造蛋白酶的方法。解决方案：制备蛋白酶的方法的特征在于：培养具有蛋白酶活性的蛋白酶活性，包括特定氨基酸序列等，编码蛋白质的基因等，包含该基因的重组载体，转化体包括重组体载体和转化体; 从所得培养物中回收蛋白酶。版权所有（C）2013，JPO＆INPIT

9. 发明专利

JP2003164284A NEW ENDOGLUCANASE AND ENDOGLUCANASE GENE 审中-公开
公开(公告)号：JP2003164284A
公开(公告)日：2003-06-10
申请号：JP2001365827
申请日：2001-11-30
申请人： NODA INST FOR SCIENTIFIC RES , KIKKOMAN CORP
发明人： UMITSUKE GENRYU , HARA SEIICHI , HATAMOTO OSAMU , MASUDA TSUTOMU , KOYAMA TAIJI , MATSUDA TOSHIBUMI , ITO KOTARO , TAKAHASHI OSAMU , MATSUSHIMA KENICHIRO
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/42
摘要： PROBLEM TO BE SOLVED: To provide a new endoglucanase, a gene encoding the enzyme, a recombinant vector containing the gene and a transformant transformed by the vector and to provide a method for producing the endoglucanase by using the transformant. SOLUTION: This invention provides a protein containing a specific amino acid sequence, the endoglucanase gene encoding the protein, the endoglucanase gene containing DNA containing a base sequence represented by the other specific amino acid sequence, the recombinant vector containing the gene, the transformant containing the recombinant vector and the method for producing the endoglucanase by collecting the endoglucanase protein from culture products obtained by culturing the transformant. COPYRIGHT: (C)2003,JPO

10. 发明专利

JP2003250581A PROLIDASE, GENE THEREOF AND METHOD FOR PRODUCING PROLIDASE 审中-公开
公开(公告)号：JP2003250581A
公开(公告)日：2003-09-09
申请号：JP2002283795
申请日：2002-09-27
申请人： KIKKOMAN CORP , NODA INST FOR SCIENTIFIC RES
发明人： ITO KOUTARO , NAKAHARA TAKEHARU , KOYAMA TAIJI , MATSUDA TOSHIBUMI , TAKAHASHI OSAMU , MATSUSHIMA KENICHIRO , UMITSUKE GENRYU , MASUDA TSUTOMU
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/62
摘要： PROBLEM TO BE SOLVED: To improve the protein engineering of prolidase and improve microorganisms which are used in the production of enzymes for food processing and in the production of brewed foods. SOLUTION: This invention provides a protein that comprises a specific amino acid sequence represented by a specific sequence, a prolidase gene that encodes the protein, a prolidase gene that comprises a base sequence represented by a specific sequence, a recombinant DNA formed by transducing these genes into a vector DNA, a transformer or a transductant containing the recombinant DNA, and a method for producing the prolidase using the transformant or the transductant. COPYRIGHT: (C)2003,JPO

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