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1. 发明专利

JP2003164286A MANNANASE AND MANNANASE GENE 审中-公开
公开(公告)号：JP2003164286A
公开(公告)日：2003-06-10
申请号：JP2001365829
申请日：2001-11-30
申请人： NODA INST FOR SCIENTIFIC RES , KIKKOMAN CORP
发明人： UMITSUKE GENRYU , HARA SEIICHI , HATAMOTO OSAMU , MASUDA TSUTOMU , KOYAMA TAIJI , MATSUDA TOSHIBUMI , ITO KOTARO , TAKAHASHI OSAMU , MATSUSHIMA KENICHIRO
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/24
摘要： PROBLEM TO BE SOLVED: To provide a new mannanase, a gene encoding the enzyme, a recombinant vector containing the gene and a transformant transformed by the vector and to provide a method for producing the mannanase by using the transformant. SOLUTION: This invention provides a protein containing a specific amino acid sequence, the mannanase gene encoding the protein, the mannanase gene containing DNA containing a specific base sequence, the recombinant vector containing the gene, the transformant containing the recombinant vector and the method for producing the mannanase by collecting the mannanase protein from culture products obtained by culturing the transformant. COPYRIGHT: (C)2003,JPO

2. 发明专利

JP2003164284A NEW ENDOGLUCANASE AND ENDOGLUCANASE GENE 审中-公开
公开(公告)号：JP2003164284A
公开(公告)日：2003-06-10
申请号：JP2001365827
申请日：2001-11-30
申请人： NODA INST FOR SCIENTIFIC RES , KIKKOMAN CORP
发明人： UMITSUKE GENRYU , HARA SEIICHI , HATAMOTO OSAMU , MASUDA TSUTOMU , KOYAMA TAIJI , MATSUDA TOSHIBUMI , ITO KOTARO , TAKAHASHI OSAMU , MATSUSHIMA KENICHIRO
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/42
摘要： PROBLEM TO BE SOLVED: To provide a new endoglucanase, a gene encoding the enzyme, a recombinant vector containing the gene and a transformant transformed by the vector and to provide a method for producing the endoglucanase by using the transformant. SOLUTION: This invention provides a protein containing a specific amino acid sequence, the endoglucanase gene encoding the protein, the endoglucanase gene containing DNA containing a base sequence represented by the other specific amino acid sequence, the recombinant vector containing the gene, the transformant containing the recombinant vector and the method for producing the endoglucanase by collecting the endoglucanase protein from culture products obtained by culturing the transformant. COPYRIGHT: (C)2003,JPO

3. 发明专利

JP2003164285A NEW ENDOGLUCANASE AND ENDOGLUCANASE GENE 审中-公开
公开(公告)号：JP2003164285A
公开(公告)日：2003-06-10
申请号：JP2001365828
申请日：2001-11-30
申请人： NODA INST FOR SCIENTIFIC RES , KIKKOMAN CORP
发明人： UMITSUKE GENRYU , HARA SEIICHI , HATAMOTO OSAMU , MASUDA TSUTOMU , KOYAMA TAIJI , MATSUDA TOSHIBUMI , ITO KOTARO , TAKAHASHI OSAMU , MATSUSHIMA KENICHIRO
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/42
摘要： PROBLEM TO BE SOLVED: To provide a new endoglucanase, a gene encoding the enzyme, a recombinant vector containing the gene and a transformant transformed by the vector and to provide a method for producing the endoglucanase by using the transformant. SOLUTION: This invention provides a protein containing a specific amino acid sequence, the endoglucanase gene encoding the protein, the endoglucanase gene containing DNA containing a specific base sequence, the recombinant vector containing the gene, the transformant containing the recombinant vector and the method for producing the endoglucanase by collecting the endoglucanase protein from culture products obtained by culturing the transformant. COPYRIGHT: (C)2003,JPO

4. 发明专利

JP2003235584A TRANSCRIPTION FACTOR, TRANSCRIPTION FACTOR GENE, RECOMBINANT VECTOR CONTAINING TRANSCRIPTION FACTOR GENE, ASPERGILLUS ORYZAE TRANSFORMED WITH THE VECTOR AND METHOD FOR USING ASPERGILLUS ORYZAE 审中-公开
公开(公告)号：JP2003235584A
公开(公告)日：2003-08-26
申请号：JP2002045090
申请日：2002-02-21
申请人： NODA INST FOR SCIENTIFIC RES , NAT INST OF ADV IND & TECHNOL
发明人： UMITSUKE GENRYU , HATAMOTO OSAMU , HARA SEIICHI , MASUDA TSUTOMU , SANO MOTOAKI , MACHIDA MASAYUKI
IPC分类号： C12N15/09 , C07K14/38 , C12N1/14 , C12N1/15 , C12N9/50 , C12N9/62 , C12N15/31 , C12P21/00 , C12R1/69
摘要： PROBLEM TO BE SOLVED: To obtain a transcription factor having function of controlling expression of sulfur assimilation-based gene of Aspergillus oryzae in culture and a gene encoding the protein. SOLUTION: The protein contains a specific amino acid sequence. The gene encodes a specific amino acid sequence or contains a specific base sequence. COPYRIGHT: (C)2003,JPO

5. 发明专利

JP2000026499A NEW PEPTIDE, ANTIMICROBIAL AGENT, NEW PEPTIDE GENE, NEW RECOMBINANT DNA AND PRODUCTION OF NEW PEPTIDE 失效
公开(公告)号：JP2000026499A
公开(公告)日：2000-01-25
申请号：JP19719098
申请日：1998-07-13
申请人： NATL INST OF SERICULTURAL & EN , NODA INST FOR SCIENTIFIC RES
发明人： YAMAKAWA MINORU , MIYANOSHITA AKITA , HARA SEIICHI
IPC分类号： C12N15/09 , A01N37/44 , A61K38/00 , A61P31/04 , C07K14/435
摘要： PROBLEM TO BE SOLVED: To obtain a new peptide comprising a peptide having a specific amino acid sequence, existing in the body fluid of beetle, exhibiting a strongly antibacterial action on Escherichia coli, Staphylococcus aureus, etc., useful as an antiseptic for foods, an antimicrobial agent for treatment, etc. SOLUTION: This new peptide comprises an amino acid sequence of the formula or comprises an amino acid sequence in which one or a plurality of amino acids are added, deleted or replaced in the amino acid sequence of the formula and has antimicrobial activity, has excellent antimicrobial activity against Escherichia coli, Staphylococcus aureus, etc., and is useful as an antiseptic for foods, an antimicrobial agent for medical treatment, etc. The peptide is obtained by injecting Escherichia coli into a beetle larva to induce antimicrobial activity, cutting a foot to release a body fluid, adding aprotinin to the body fluid, centrifuging, heat-treating the supernatant liquid, subjecting the heat-treated liquid to reversed phase chromatography, eluting an adsorbed fraction, collecting an antimicrobial active fraction and purifying it by high- performance liquid chromatography.

6. 发明专利

JP2011239681A Koji mold alkaline protease promoter 审中-公开
标题翻译： KOJI模具碱性蛋白酶促进剂
公开(公告)号：JP2011239681A
公开(公告)日：2011-12-01
申请号：JP2008240116
申请日：2008-09-19
申请人： Kikkoman Corp , キッコーマン株式会社
发明人： HARA SEIICHI
IPC分类号： C12N15/09 , C12N1/15 , C12P21/02
CPC分类号： C12N15/80 , C12N9/62 , C12P21/02
摘要： PROBLEM TO BE SOLVED: To provide a strong promoter by clarifying the relation between the length of the upstream region of koji mold alkaline protease gene and the strength of the promoter activity.SOLUTION: There are provided an alkaline protease promoter comprising the upstream region of the koji mold alkaline protease gene and having a length longer than about 1.1 kb and equal to or less than about 2.5 kb, a gene expression unit containing the promoter, a homologous recombination cassette containing the gene expression unit, and a transformant microorganism and the like prepared by transferring the alkaline protease promoter into the genomic gene of a host microorganism.
摘要翻译：待解决的问题：通过澄清曲霉碱性蛋白酶基因上游区域的长度与启动子活性的强度之间的关系来提供强启动子。提供了一种碱性蛋白酶促进剂，其包含曲霉碱性蛋白酶基因的上游区域，长度大于约1.1kb且等于或小于约2.5kb，含有启动子的基因表达单元，含有基因表达单位的同源重组盒，以及通过将碱性蛋白酶启动子转移到宿主微生物的基因组基因中而制备的转化体微生物等。版权所有（C）2012，JPO＆INPIT

7. 发明专利

JP2006340641A KOJI MOLD IN WHICH NEUTRAL TREHALASE GENE IS BROKEN 审中-公开
公开(公告)号：JP2006340641A
公开(公告)日：2006-12-21
申请号：JP2005168129
申请日：2005-06-08
申请人： KIKKOMAN CORP
发明人： MATSUDA TOSHIBUMI , HARA SEIICHI , KOYAMA TAIJI , UMITSUKE GENRYU , HATAMOTO OSAMU
IPC分类号： C12N1/14 , C12N15/09 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide a koji mold which vigorously proliferates even when water content charged to raw materials is lower than a conventional water content and having rapid growing rate in a method for producing koji for brewing and to provide the method for producing the koji for brewing by using the koji mold. SOLUTION: The present invention provides the koji mold in which decomposition ability of trehalose in a cell or a conidium is deficient, particularly the koji mold in which a gene involving decomposition of trehalose, e.g., neutral trehalase gene is broken and provides the method for producing the koji for brewing by inoculating the koji mold into a protein raw material derived from a plant or an animal and culturing the koji mold. COPYRIGHT: (C)2007,JPO&INPIT

8. 发明专利

JP2007228819A Offensive/musty odor-low-productive koji mold 审中-公开
标题翻译：有风味/必须气味低的KOJI模具
公开(公告)号：JP2007228819A
公开(公告)日：2007-09-13
申请号：JP2006051520
申请日：2006-02-28
申请人： Kikkoman Corp , キッコーマン株式会社
发明人： TAKECHI JUNYA , HARA SEIICHI , HANYA YOSHISATO , KOYAMA TAIJI
IPC分类号： C12N1/15 , A23L5/20 , A23L27/50 , C12N15/09 , C12Q1/02
摘要： PROBLEM TO BE SOLVED: To provide well-flavored high-quality food/drink slight in offensive and stuffy odor. SOLUTION: A koji mold is provided, which is produced by a gene manipulation such as gene disruption and in which a branched-chain amino acid aminotransferase gene or branched-chain α-keto-acid dehydrogenase gene does not function. A method for screening the above koji mold is also provided, being based on the phenotype of a koji mold in which the above gene is deleted, or comprising assaying the activity of the above-mentioned enzyme. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：提供味道好的高品质食品/饮料，有点冒险和闷热的气味。提供了通过诸如基因破坏等基因操作产生的曲线霉菌，其中支链氨基酸氨基转移酶基因或支链α-酮酸脱氢酶基因不起作用。还提供了用于筛选上述曲霉的方法，其基于上述基因缺失的曲霉的表型，或包括测定上述酶的活性。版权所有（C）2007，JPO＆INPIT

9. 发明专利

JP2011147348A Carboxypeptidase gene 审中-公开
标题翻译：羧基酶基因
公开(公告)号：JP2011147348A
公开(公告)日：2011-08-04
申请号：JP2010008619
申请日：2010-01-19
申请人： Kikkoman Corp , キッコーマン株式会社
发明人： YOSHIMURA TARO , HARA SEIICHI , HATTORI SHOHEI
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/62
摘要： PROBLEM TO BE SOLVED: To provide a novel carboxypeptidase capable of decomposing peptides existing in soy sauce and a seasoning agent containing an enzymatically decomposed material. SOLUTION: There is disclosed the following carboxypeptidase of (a) or (b): (a) a protein consisting of a specific amino acid sequence; and (b) a protein consisting of an amino acid sequence in which one or more amino acids in the amino acid sequence of (a) are deleted, substituted or added, and having carboxypeptidase activity. The novel carboxypeptidase can efficiently decompose a raw protein material and improve amination rate. COPYRIGHT: (C)2011,JPO&INPIT

10. 发明专利

JP2010029183A Peptide-containing seasoning material 有权
标题翻译：含肽包装材料
公开(公告)号：JP2010029183A
公开(公告)日：2010-02-12
申请号：JP2009153239
申请日：2009-06-29
申请人： Kikkoman Corp , キッコーマン株式会社
发明人： NAKAHARA TAKEHARU , UCHIDA RIICHIRO , HANADA YOICHI , ENDO YOSHITOMO , TANIZAWA SHIGENORI , AOTA HITOMI , HARA SEIICHI
IPC分类号： A23L1/227
CPC分类号： A23L27/50
摘要： PROBLEM TO BE SOLVED: To provide a fermented seasoning material easily taken orally without having a bitter taste peculiar to a peptide, although containing much peptides having useful physiological properties. SOLUTION: The fermented seasoning material is obtained by mashing after adding salt water to a koji mold culture or a material obtained by mixing the koji mold culture with a plant-originated proteinous raw material, and controlling a leucine aminopeptidase I activity as ≤1.0 U/ml and a leucine aminopeptidase II activity as ≤0.5 U/ml in mashed material liquid within 10 days after the mashing. COPYRIGHT: (C)2010,JPO&INPIT

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