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1. 发明专利

JP2013192537A Efficient fusion method of aspergillus cell line 有权
标题翻译： ASPERGILLUS细胞系的有效融合方法
公开(公告)号：JP2013192537A
公开(公告)日：2013-09-30
申请号：JP2012065157
申请日：2012-03-22
申请人： Univ Of Tokyo , 国立大学法人東京大学 , Noda Institute For Scientific Research , 公益財団法人野田産業科学研究所
发明人： KITAMOTO KATSUHIKO , MARUYAMA JUNICHI , WADA RYUTA , KOYAMA TAIJI , JIN FENG JIE
IPC分类号： C12N15/02 , C12N1/15 , C12N15/09 , C12Q1/02
摘要： PROBLEM TO BE SOLVED: To provide a method to identify completion of sexual reproduction when implementing sexual reproduction experiments using Aspergillus cell line, and a method to improve efficiency in fusion of the cell line.SOLUTION: There are provided a method to increase fusion efficiency of Aspergillus cell lines by increasing expression of sclerotium formation promoter SclR in at least one of the cell lines to be fused, in fusion of two kinds of Aspergillus cell lines (hypha); and a method to identify fusion cells based on criteria where two Aspergillus cell lines to be fused are transformants which are distinguishable by mutually distinctive phenotypes, and in fused cells, the phenotypes are complementing each other and/or the fused cells have two phenotypes, in fusion of two kinds of Aspergillus cell lines (hypha).
摘要翻译：要解决的问题：提供一种在使用曲霉菌细胞系实施有性繁殖实验时确定有性生殖完成的方法，以及提高细胞系融合效率的方法。提供了一种提高融合效率的方法曲霉菌细胞系通过增加至少一种待融合的细胞系中菌核形成启动子SclR的表达，融合两种曲霉菌细胞株（菌丝）; 以及基于标准的鉴定融合细胞的方法，其中待融合的两个曲霉细胞系是通过相互独特的表型可区分的转化体，在融合的细胞中，表型彼此互补和/或融合的细胞具有两种表型，融合两种曲霉菌细胞株（菌丝）。

2. 发明专利

JP2014039559A Transformant not producing cyclopiazonic acid and preparation method of the same 有权
标题翻译：转化不生产环丙烯酸及其制备方法
公开(公告)号：JP2014039559A
公开(公告)日：2014-03-06
申请号：JP2013213095
申请日：2013-10-10
申请人： Noda Institute For Scientific Research , 公益財団法人野田産業科学研究所
发明人： TOKUOKA MASAFUMI , TAKAHASHI OSAMU , KOYAMA TAIJI , FUJII ISAO , SESHIME YASUYO
IPC分类号： C12N15/09 , C12N1/15
CPC分类号： C12N15/52 , C12P17/182 , C12Q1/6895 , C12Q2600/156
摘要： PROBLEM TO BE SOLVED: To provide a rapid, accurate and genetic engineering method for detecting producing ability of a mycotoxin cyclopiazonic acid in strains of the genus Aspergillus or the genus Penicillium etc., and to provide a transformant not producing cyclopiazonic acid, a nerve toxin, or its precursor cyclo-acetoacetyl-L-tryptophan (CAT), since some strains of Aspergillus oryzae used for production of soy source, miso (fermented bean paste) or Japanese sake, or Penicillium camenberti used for cheese production produce cyclopiazonic acid.SOLUTION: Provided is a genetic engineering method for preparing a transformant of the genus Aspergillus or the genus Penicillium transformed not to produce cyclopiazonic acid, where the transformant includes a strain not producing CAT and a strain not expressing polyketide synthase/non-ribosomal peptide synthetase. Also provided is a transformant not producing cyclopiazonic acid obtained by the preparation method.
摘要翻译：要解决的问题：提供一种快速，准确和遗传的工程方法，用于检测真菌毒素环氮酸在曲霉属或青霉属等菌株中的产生能力，并提供不产生环氮酸的转化体，神经毒素，或其前体环 - 乙酰乙酰基-L-色氨酸（CAT），因为用于生产大豆源，味噌（发酵豆酱）或日本清酒或用于奶酪生产的青霉菌的一些米曲霉菌株产生环氮酸。提供了一种用于制备转化体不产生环磷酸的曲霉属或青霉属的转化体的遗传工程方法，其中转化体包括不产生CAT的菌株和不表达聚酮化合物合酶/非核糖体多肽合成酶的菌株。还提供了通过制备方法获得的不产生环硼酸的转化体。

3. 发明专利

JP2012187102A Novel protease gene, recombinant dna, and method for making protease 审中-公开
标题翻译：新型蛋白酶基因，重组DNA和制备蛋白质的方法
公开(公告)号：JP2012187102A
公开(公告)日：2012-10-04
申请号：JP2012039230
申请日：2012-02-24
申请人： Kikkoman Corp , Noda Institute For Scientific Research , キッコーマン株式会社 , 公益財団法人野田産業科学研究所
发明人： OGAWA SHINKO , SATO ATSUSHI , TAKAHASHI OSAMU , OSHIMA KENSHIRO , HATTORI SHOHEI , KOYAMA TAIJI , OGUMA TETSUYA , HANYA YOSHISATO
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/62
摘要： PROBLEM TO BE SOLVED: To provide: a novel protease capable of decomposing a protein present in a raw material such as a soy sauce and an enzyme decomposing seasoning; a novel gene encoding the protease; a recombinant DNA including the gene; a transformant or a transductant including the recombinant DNA; and a method for making the protease using the transformant and the transductant.SOLUTION: The method for making the protease is characterized by: culturing the proteins having protease activity including a specific amino acid sequence or others, a gene encoding the protein or others, the recombinant vector including the gene, the transformant including the recombinant vector, and the transformant; and recovering the protease from the obtained cultured substance.
摘要翻译：待解决的问题：提供：能够分解存在于原料如酱油和酶分解调味料中的蛋白质的新型蛋白酶; 编码蛋白酶的新基因; 包含该基因的重组DNA; 包含重组DNA的转化体或转导体; 以及使用转化体和转导体制造蛋白酶的方法。解决方案：制备蛋白酶的方法的特征在于：培养具有蛋白酶活性的蛋白酶活性，包括特定氨基酸序列等，编码蛋白质的基因等，包含该基因的重组载体，转化体包括重组体载体和转化体; 从所得培养物中回收蛋白酶。版权所有（C）2013，JPO＆INPIT

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