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1. 发明专利

JP2013021961A Cell evaluation method, cell evaluation apparatus, and cell evaluation program 审中-公开
标题翻译：细胞评估方法，细胞评价装置和细胞评估方案
公开(公告)号：JP2013021961A
公开(公告)日：2013-02-04
申请号：JP2011159028
申请日：2011-07-20
申请人： Hamamatsu Photonics Kk , 浜松ホトニクス株式会社
发明人： KATAOKA TAKUJI , SUGIYAMA NORIKAZU
IPC分类号： C12M1/34 , C12N5/071 , G01N21/17 , G01N33/483
摘要： PROBLEM TO BE SOLVED: To evaluate pumping power of myocardial cell in high accuracy.SOLUTION: There is provided a cell measurement method is a cell evaluation method to evaluate the pumping power of a myocardial cell C, and includes an image acquiring step S1 to acquire an optical image data 60 of the myocardial cell C attached on a transparent specimen case, a region setting step S2 to set an analyzing region RC indicating a region occupied by the myocardial cell C based on luminance values of pixels of the optical image data 60, a data acquisition step S3 to acquire at least one of thickness data showing the temporal change of the thickness of the myocardial cell C and areal data showing the temporal change of the area of the region occupied by the myocardial cell C based on the pixels of the analyzing region RC, and an evaluation step S6 to evaluate the pumping power of the myocardial cell C based on at least one of the areal data and the thickness data acquired by the data acquisition step S3.
摘要翻译：要解决的问题：以高精度评估心肌细胞的泵浦功率。解决方案：提供了一种细胞测量方法，其是评估心肌细胞C的泵浦功率的细胞评估方法，并且包括图像获取步骤S1，以获取附着在心肌细胞C上的心肌细胞C的光学图像数据60 透明样品盒，区域设定步骤S2，根据光学图像数据60的像素的亮度值设定指示心肌细胞C所占据的区域的分析区域RC，获取厚度数据显示了基于分析区域RC的像素的心肌细胞C的厚度的时间变化和显示心肌细胞C占据的区域的面积的时间变化的面积数据，以及评估步骤S6，以评估泵送基于通过数据获取步骤S3获取的面积数据和厚度数据中的至少一个，心肌细胞C的功率。版权所有（C）2013，JPO＆INPIT

2. 发明专利

JP2012231709A Cell analysis method, cell analysis device and cell analysis program 有权
标题翻译：细胞分析方法，细胞分析装置和细胞分析方案
公开(公告)号：JP2012231709A
公开(公告)日：2012-11-29
申请号：JP2011101377
申请日：2011-04-28
申请人： Hamamatsu Photonics Kk , 浜松ホトニクス株式会社
发明人： SUGIYAMA NORIKAZU , TAKESHIMA TOMOCHIKA , KANEKO KOICHI
IPC分类号： C12Q1/02 , C12M1/34 , G01N21/17
CPC分类号： G06K9/00147 , C12M41/36 , G01N21/6458 , G01N33/5026 , G02B21/14 , G06K9/00134 , G06K9/0014
摘要： PROBLEM TO BE SOLVED: To provide a cell analysis method, a cell analysis device and a cell analysis program which is quantitative, can be automated and can determine the state of a stem cell colony with high accuracy.SOLUTION: The cell analysis method for the cell analysis device D that uses an optical path length image of a cell colony constituted of a large number of cells to analyze the cell colony includes: a step wherein an acquisition unit D1 of the cell analysis device D acquires an optical path length image of a cell colony; a step wherein an extraction unit D2 of the cell analysis device D extracts, from the acquired optical path length image, circular shapes each corresponding to a cell nucleus of a cell; a step wherein a comparison unit D3 of the cell analysis device D compares, for each extracted circular shape, an inner optical path length and an outer optical path length thereof; and a step wherein an analysis unit D4 of the cell analysis device D analyzes the cell colony on the basis of the comparison result.
摘要翻译：要解决的问题：为了提供细胞分析方法，细胞分析装置和定量的细胞分析程序，可以自动化并且可以高精度地确定干细胞集落的状态。解决方案：使用由大量细胞构成的细胞集落的光路长度图像来分析细胞集落的细胞分析装置D的细胞分析方法包括：其中细胞的获取单元D1 分析装置D获取细胞集落的光路长度图像; 细胞分析装置D的提取部D2从获取的光路长度图像中提取各自对应于细胞的细胞核的圆形的步骤; 对于每个提取的圆形形状，细胞分析装置D的比较单元D3对其内部光程长度和外部光程长度进行比较的步骤; 以及其中细胞分析装置D的分析单元D4基于比较结果分析细胞群的步骤。版权所有（C）2013，JPO＆INPIT

3. 发明专利

JP2011232056A Apparatus and method for detecting change in membrane potential 有权
标题翻译：用于检测膜电位变化的装置和方法
公开(公告)号：JP2011232056A
公开(公告)日：2011-11-17
申请号：JP2010100284
申请日：2010-04-23
申请人： Hamamatsu Photonics Kk , 浜松ホトニクス株式会社
发明人： SUGIYAMA NORIKAZU , KATAOKA TAKUJI , IKEDA TAKAHIRO
IPC分类号： G01N21/27 , G01N21/03
CPC分类号： G01N21/45 , C12Q1/02 , G01N21/253
摘要： PROBLEM TO BE SOLVED: To provide an apparatus and a method for detecting a change in a membrane potential capable of detecting the change in the membrane potential of a cell in a noninvasive method without labeling the cell membrane.SOLUTION: The apparatus for detecting the change in the membrane potential 1 includes: a light source 106 for reflection interference measurement; a holding section 103 for holding a transparent member 102a mounted with a cell 101; a camera 110 for reflection interference detection for imaging the reflected light which has been emitted from the light source 106 for reflection interference measurement and has been reflected from the cell 101 through the transparent member 102a to form a reflection interference image; and an analysis section 202 for calculating a parameter dI concerning adhesion between the cell 101 and the transparent member 102a from the reflection interference image and detecting the change in the membrane potential of the cell 101 based on the change in the parameter dI.
摘要翻译：要解决的问题：提供一种用于检测能够以非侵入性方法检测细胞膜电位变化而不标记细胞膜的膜电位变化的装置和方法。解决方案：用于检测膜电位1的变化的装置包括：用于反射干涉测量的光源106; 用于保持安装有单元101的透明构件102a的保持部103; 用于对从光源106发射的用于反射干涉测量的反射光成像的反射干涉检测用摄像机110，并通过透明构件102a从单元101反射，形成反射干涉图像; 以及分析部202，用于根据反射干涉图像计算关于单元101和透明构件102a之间的粘附的参数dI，并且基于参数dI的变化来检测单元101的膜电位的变化。版权所有（C）2012，JPO＆INPIT

4. 发明专利

JP2010139326A Observation device and observation method 有权
标题翻译：观察装置和观察方法
公开(公告)号：JP2010139326A
公开(公告)日：2010-06-24
申请号：JP2008314780
申请日：2008-12-10
申请人： Hamamatsu Photonics Kk , 浜松ホトニクス株式会社
发明人： YAMAUCHI TOYOHIKO , SUGIYAMA NORIKAZU , IWAI HIDENAO
IPC分类号： G01N21/27 , G01J9/00
摘要： PROBLEM TO BE SOLVED: To provide an observation device and an observation method capable of acquiring detailed information of an object such as a cell. SOLUTION: This observation device 1 for observing the surface or the inside of an observation object 9 includes light sources 11, 12, lenses 21-25, an aperture 31, an optical multiplexer 41, an optical branching filter 42, a half mirror 43, an imaging section 51, an analyzing section 52, a displaying section 53, a light receiving section 61, a displacement detecting section 62, a piezo actuator 71, a driving section 72, a mirror 73, a stage 81, a driving section 82, and a controlling section 90. Images of an intensity component and a phase component of second reflected light generated in the observation object are determined based on an interference light image imaged by the imaging section, and an observation range is predetermined, wherein the interference light image is obtained, which is generated by the second reflected light generated by reflection of second branched light by a film of the observation object based on the image of the phase component, and an image at least in the observation range in the image of the intensity component is displayed by the image in time series, to thereby observe behavior of the film of the observation object and granule near the film. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：解决的问题：提供能够获取诸如单元的对象的详细信息的观察装置和观察方法。解决方案：用于观察观察对象9的表面或内部的观察装置1包括光源11,12，透镜21-25，孔31，光复用器41，光分路滤波器42，半镜43，成像部51，分析部52，显示部53，受光部61，位移检测部62，压电致动器71，驱动部72，反射镜73，台81，驱动部分82和控制部分90.基于由成像部分成像的干涉光图像确定观察对象中产生的第二反射光的强度分量和相位分量的图像，并且预测观察范围，其中获得由基于相位分量的图像的观察对象的膜通过第二分支光的反射产生的第二反射光产生的干涉光图像，以及至少在t 通过时间序列中的图像显示强度分量的图像中的观察范围，从而观察膜附近的观察对象和颗粒的膜的行为。版权所有（C）2010，JPO＆INPIT

5. 发明专利

JP2014153154A Aggregated cell evaluation method and aggregated cell evaluation device 有权
标题翻译：聚集细胞评估方法和聚集细胞评估装置
公开(公告)号：JP2014153154A
公开(公告)日：2014-08-25
申请号：JP2013022234
申请日：2013-02-07
申请人： Hamamatsu Photonics Kk , 浜松ホトニクス株式会社
发明人： IWAI HIDENAO , KATAOKA TAKUJI , YAMAMOTO SATOSHI , SUGIYAMA NORIKAZU , YAMAUCHI TOYOHIKO , GOTO KENTARO
IPC分类号： G01N21/47 , G01B11/00 , G01N21/17 , G01P3/36
CPC分类号： G01N33/4833 , G01B11/00 , G01N21/47 , G01N21/49 , G01N2021/4707 , G01N2021/479
摘要： PROBLEM TO BE SOLVED: To provide a method and device capable of easily evaluating motion of aggregated cells.SOLUTION: An aggregated cells evaluation device 1 includes a laser beam source 10, a speckle image acquisition part 20, an SC calculation part 30, an evaluation part 40, and a storage part 50. The speckle image acquisition part 20 acquires a two-dimensional speckle image caused by forward scattered light generated in aggregated cells 90 by irradiation of the aggregated cells 90 with laser beam output from the laser beam source 10. The SC calculation part 30 calculates a speckle contrast value Kof a speckle image Iat each time tto obtain a maximum value Kamong speckle contrast values Kto Kand normalizes the speckle contrast value Kat each time tby the maximum value Kto obtain a normalized speckle contrast value K'. The evaluation part 40 evaluates motion of the aggregated cells 90 on the basis of the normalized speckle contrast value K' at each time t.
摘要翻译：要解决的问题：提供能够容易地评估聚集电池的运动的方法和装置。解决方案：聚集电池评估装置1包括激光束源10，散斑图像获取部20，SC计算部30，评价部分40和存储部分50.斑点图像获取部分20通过从激光束源10输出的激光束照射聚集单元90，获得由聚集单元90中产生的正向散射光引起的二维散斑图像。 SC计算部30计算每次得到最大值Kamong散斑对比度值Kto K的斑点图像Iat的散斑对比度值K，并且每次以最大值K为标准化散斑对比度值即可得到归一化斑点对比度值K'。评价部40基于每个时刻t的归一化斑点对比度值K'来评价聚集单元90的运动。

6. 发明专利

JP2011147400A Cell discrimination method, reference data formation method for cell discrimination, and cell distinguishing device 有权
标题翻译：细胞鉴别方法，细胞鉴别的参考数据形成方法和细胞鉴别装置
公开(公告)号：JP2011147400A
公开(公告)日：2011-08-04
申请号：JP2010012125
申请日：2010-01-22
申请人： Hamamatsu Photonics Kk , Hamamatsu Univ School Of Medicine , 国立大学法人浜松医科大学 , 浜松ホトニクス株式会社
发明人： TERAKAWA SUSUMU , SAKURAI KOJI , SUGIYAMA NORIKAZU , YAMAUCHI TOYOHIKO , IWAI HIDENAO
IPC分类号： C12Q1/02 , C12M1/00 , C12M1/34
CPC分类号： C12M47/04
摘要： PROBLEM TO BE SOLVED: To provide a cell discrimination method, which can discriminate cells without invasion and with high precision, a method of production of reference data for cell discrimination and a device for distinguishing cells.
SOLUTION: The method includes a reference data generation step of measuring the vibration of a subject of cells with a quantitative phase microscope for a certain fixed time, analyzing the measured results for frequencies to produce reference data for the discrimination of the cells, and a step of distinguishing specimen cells by comparing the results of measuring the vibration of cells with the quantitative phase microscope for the certain fixed time duration and the frequency analysis to be conducted for the results of the measurement with the reference data produced in the reference data generation step.
COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：解决的问题：为了提供能够以不侵入且高精度地区分细胞的细胞鉴别方法，用于产生用于细胞鉴别的参考数据的方法和用于区分细胞的装置。解决方案：该方法包括参考数据生成步骤，用于在一定固定时间内用定量相位显微镜测量细胞对象的振动，分析频率的测量结果以产生用于鉴别细胞的参考数据，以及通过将参考数据产生的参考数据与测量结果进行比较，比较测定细胞振动的结果与定量相位显微镜的特定固定持续时间和频率分析的步骤生成步骤。版权所有（C）2011，JPO＆INPIT

7. 发明专利

JP2004283776A Production apparatus and production method for functional water 有权
标题翻译：功能水的生产设备和生产方法
公开(公告)号：JP2004283776A
公开(公告)日：2004-10-14
申请号：JP2003081498
申请日：2003-03-24
申请人： Hamamatsu Photonics Kk , 浜松ホトニクス株式会社
发明人： KATAOKA TAKUJI , SUGIYAMA NORIKAZU , FUKUSHIMA MASANORI , MORI KANEO
IPC分类号： C02F1/30 , C02F1/48
摘要： PROBLEM TO BE SOLVED: To provide a production apparatus for functional water which has cell activation effects, increases immune functions, cell proliferation capacity, and ATP (adenosine triphosphate) content in cells, and brings beneficial effects on living bodies such as human body, and a production method therefor.
SOLUTION: The production apparatus for the functional water has a magnetic shielding means for forming a magnetic shielding space by shielding a magnetic field from the outside of a predetermined space, a water storage container for holding water to be treated inside the magnetic shielding space, a direct current magnetic field application means and a low-frequency micromagnetic field application means for obtaining the functional water by simultaneously applying steady direct current magnetic field and low-frequency micromagnetic field to the water to be treated inside the water storage container, and an angle adjustment means for holding the direct current magnetic field application means and the low-frequency micromagnetic field application means so that the angle between a first axis corresponding to the direction of the direct current magnetic field and a second axis corresponding to the direction of the low-frequency micromagnetic field becomes a predetermined angle.
COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：为了提供具有细胞活化作用的功能性水的生产装置，增加细胞中的免疫功能，细胞增殖能力和ATP（三磷酸腺苷）含量，并且对人体如人体产生有益效果身体及其生产方法。解决方案：功能水的制造装置具有磁屏蔽装置，用于通过屏蔽来自预定空间的外部的磁场形成磁屏蔽空间，用于在磁屏蔽内保持被处理水的储水容器空间，直流磁场施加装置和低频微磁场施加装置，用于通过向储水容器内的待处理水施加稳定的直流磁场和低频微磁场来获得功能水;以及用于保持直流磁场施加装置和低频微磁场施加装置的角度调节装置，使得对应于直流磁场方向的第一轴线和对应于直流磁场施加装置的方向的第二轴线之间的角度低频微磁场变为预定角度。版权所有（C）2005，JPO＆NCIPI

8. 发明专利

JPH11258526A LIGHT QUANTITY ADJUSTING METHOD/DEVICE 失效
公开(公告)号：JPH11258526A
公开(公告)日：1999-09-24
申请号：JP6558598
申请日：1998-03-16
申请人： HAMAMATSU PHOTONICS KK
发明人： TERADA HIROTOSHI , TOMITA TAKAFUMI , SUGIYAMA NORIKAZU
IPC分类号： H01S3/10 , G02B26/02
摘要： PROBLEM TO BE SOLVED: To eliminate the adjustment of the optical axis of outgoing light and to be able to continuously change the light quantity. SOLUTION: In the device 10, same polarizing beam splitters 14 and 15 different in transmission quantity corresponding to polarization component are symmetrically arranged against a plane orthogonal to the rotary axis of an inner lens barrel 12 arranged in an outer lens barrel 11 so that it can freely rotate by inclining them at an angle θ by using a holding member 13. When an optical axis makes the linear polarizing light from a laser light source 1 enter so that it may be matched with the rotary axis of the inner lens barrel 12 and the inner lens barrel 12 is rotated to a desired position, the rate of the polarization components of laser light beams which are made incident on the polarizing beam splitters 14 and 15 changes in accordance with a rotary angle. Thus, the light quantity of outgoing light continuously changes and the optical axis shift and the angle blurring of the beam splitters 14 and 15 are canceled by each other. Consequently, an outgoing optical axis is always matched with an incident optical axis.

9. 发明专利

JP2001141735A POSITIONING DEVICE FOR CIRCULATING NOZZLE 失效
公开(公告)号：JP2001141735A
公开(公告)日：2001-05-25
申请号：JP32691899
申请日：1999-11-17
申请人： HAMAMATSU PHOTONICS KK
发明人： SUGIYAMA NORIKAZU , TANABE HIROSHI
IPC分类号： H01J37/20 , C12M1/00 , G01N37/00 , G02B21/26
摘要： PROBLEM TO BE SOLVED: To provide a positioning device for circulating nozzle capable of easily replacing a sample vessel without impairing the reproducibility of the position of a circulating nozzle. SOLUTION: In this positioning device for circulating nozzle 1 for retaining a circulating nozzle N for injecting a liquid to a vessel (Petri dish or the like) or discharging the liquid from the vessel, a nozzle retaining member 3 for retaining the circulating nozzle N is provided on a base part 2 fixable on a prescribed reference plane (the stage of a microscope). According to this, the circulating nozzle N can be fixed to the prescribed reference plane. A magnet 34 is further provided on the bottom surface of the nozzle retaining member 3, and the nozzle retaining member 3 and the base part 2 formed of a magnetic material are fixed by the magnetic force. The fixing position of the nozzle retaining member 3 on the base part 2 can be thus easily changed.

10. 发明专利

JP2019020125A 試料観察装置及び試料観察方法审中-公开
公开(公告)号：JP2019020125A
公开(公告)日：2019-02-07
申请号：JP2017135427
申请日：2017-07-11
申请人： HAMAMATSU PHOTONICS KK
发明人： SUGIYAMA NORIKAZU , MATSUBARA MASANORI , YAMAMOTO SATOSHI
IPC分类号： G01N21/64 , G02B21/06 , G02B21/36
摘要：【課題】メンブレンフィルタを通って移動した試料と移動しなかった試料との双方を観察できる試料観察装置及び試料観察方法を提供する。【解決手段】試料観察装置1は、メンブレンフィルタを透過する面状光の照射によって発生した観察光を撮像して蛍光画像データを出力する撮像部13と、第1の試料保持空間に対応する第1の領域と第2の試料保持空間に対応する第2の領域とを特定し、第1の領域に対応する第1の部分画像データと第2の領域に対応する第2の部分画像データとを生成する部分画像生成部と、これらの部分画像データに基づいて第1の観察画像データ及び第2の部分画像データを生成する観察画像生成部と、第1の観察画像データ及び第2の観察画像データに基づいて試料Sの解析を行う解析部と、を備える。【選択図】図1

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