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1. 发明专利

JP2011125302A Fat degrading bacillus and fat degrading agent 有权
标题翻译：脂肪降解木瓜和脂肪降解剂
公开(公告)号：JP2011125302A
公开(公告)日：2011-06-30
申请号：JP2009289104
申请日：2009-12-21
申请人： Fukushima Univ , Sumitomo Heavy Ind Ltd , 住友重機械工業株式会社 , 国立大学法人福島大学
发明人： SHISHIDO YOSHIKO , SUEHIRO BUNICHI , SUGIMORI DAISUKE
IPC分类号： C12N1/20 , C02F3/34
摘要： PROBLEM TO BE SOLVED: To provide a fat degrading bacillus whose optimum fat degrading conditions (temperature, pH and the like) are not within the range of optimum growth conditions (temperature, pH and the like) of BOD degrading bacillus and which has sufficient proliferation rate and fat degrading rate.
SOLUTION: There is provided a fat degrading bacillus belonging to the genus Acinetobacter, and exhibiting following mycological properties. Cell shape is micrococcus. Cell diameter is 0.8-0.9 μm. Spore existence is -. Gram staining is -. Mobility is -. Growth at 41°C is +. Catalase is +. Oxidase is -. Acid/gas production from glucose is -/-. Oxidation/fermentation of glucose is +/-. Wherein, (+) means positive and (-) means negative.
COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：为了提供其最佳脂肪降解条件（温度，pH等）不在BOD降解杆菌的最佳生长条件（温度，pH等）的范围内的脂肪降解芽孢杆菌，具有足够的增殖率和脂肪降解率。解决方案：提供属于不动杆菌属的脂肪降解芽孢杆菌，具有以下真菌学性质。细胞形状是微球菌。电池直径为0.8-0.9μm。孢子存在是 - 。革兰染色为 - 。移动是 - 。在41℃下生长为+。过氧化氢酶是+。氧化酶是 - 。来自葡萄糖的酸/气产生是 - / - 。葡萄糖的氧化/发酵为+/-。其中（+）表示正数，（ - ）表示负数。版权所有（C）2011，JPO＆INPIT

2. 发明专利

JP2011125825A Lypolytic bacteria and lipolytic agent 有权
标题翻译： LYPOLYTIC BACTERIA和LIPOLYTIC AGENT
公开(公告)号：JP2011125825A
公开(公告)日：2011-06-30
申请号：JP2009289107
申请日：2009-12-21
申请人： Fukushima Univ , Sumitomo Heavy Ind Ltd , 住友重機械工業株式会社 , 国立大学法人福島大学
发明人： SHISHIDO YOSHIKO , FUJIMOTO NORIYUKI , SUGIMORI DAISUKE
IPC分类号： C02F3/34 , C12N1/20 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide lypolytic bacteria with high propagation rate and lypolytic rate, when optimum lypolytic conditions (temperature, pH, etc.) are out of the scope of optimum growth conditions (temperatures, pH, etc.) of BOD degrading bacteria. SOLUTION: The lipolytic bacteria belongs to Pseudmonas group and indicates the following mycologic characteristics, for example: growth + at 37°C, growth + at 45°C, growth - under an anaerobic condition, nitrate reduction activity +, indole production activity -, arginine dihydrolase activity +, urease activity -, gelatin hydrolysis activity +, β-galactosidase activity -, cytochrome oxidase activity +, dextrose assimilation nature +, L-arabinose assimilation nature, D-mannose assimilation nature -, D-mannitol assimilation nature -, N-acetyl-D-Glucosamine assimilation nature +, maltose assimilation nature -, and potassium gluconate assimilation nature +, where "+" is positivity and "-" is negative. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：为了提供高增殖率和溶解率的溶血细菌，当最佳溶解条件（温度，pH等）不在最佳生长条件（温度，pH等）的范围内时 BOD降解细菌。解决方案：脂肪分解细菌属于假单胞菌属，并表示以下真菌学特征，例如：生长+ 37℃，生长+ 45℃，生长 - 在厌氧条件下，硝酸盐还原活性+吲哚生产活性 - 精氨酸二水解酶活性+，脲酶活性 - 明胶水解活性+，β-半乳糖苷酶活性 - ，细胞色素氧化酶活性+，葡萄糖同化性质+，L-阿拉伯糖同化性质，D-甘露糖同化性质，D-甘露醇同化自然 - ，N-乙酰基-D-葡萄糖胺同化性质，麦芽糖同化性质，和葡萄糖酸钾同化性质+，其中“+”为阳性，“ - ”为阴性。版权所有（C）2011，JPO＆INPIT

3. 发明专利

JP2014082991A Phospholipase d, polynucleotide, and production method of phospholipase d 有权
标题翻译：磷脂酶D，多核苷酸和磷脂酶D的生产方法
公开(公告)号：JP2014082991A
公开(公告)日：2014-05-12
申请号：JP2012234402
申请日：2012-10-24
申请人： Fukushima Univ , 国立大学法人福島大学
发明人： SUGIMORI DAISUKE , MATSUMOTO YUSAKU
IPC分类号： C12N15/09 , C12N9/16
摘要： PROBLEM TO BE SOLVED: To provide a phospholipase D which can act on a cholinergic plasmalogen, and can act on a lysophosphatidylcholine to inexpensively produce a lysophosphatidic acid, and a polynucleotide, which are obtained from a microorganism easily handled, and to provide a production method of the phospholipase D.SOLUTION: A phospholipase D which exerts an action in which a cholinergic plasmalogen and a tertiary phosphoric ester of a lysophospholipid are hydrolyzed to liberate a choline, includes either one of polypeptides described in (a) to (c): (a) a polypeptide containing an amino acid sequence consisting of a specific sequence; (b) a polypeptide containing an amino acid sequence in which one or a plurality of amino acids are substituted, inserted, deleted and/or added in the amino acid sequence consisting of the specific sequence, and having hydrolytic activity; and (c) a polypeptide containing at least 90% of homology with the amino acid sequence consisting of the specific sequence, and having hydrolytic activity.
摘要翻译：要解决的问题：提供可以作用于胆碱能浆细胞原子的磷脂酶D，并且可以作用于溶血磷脂酰胆碱以便廉价地产生溶血磷脂酸，以及从易于处理的微生物获得的多核苷酸，并提供生产方法的磷脂酶D。解决方案：发挥其中胆碱能浆果原和溶血磷脂的叔磷酸酯水解以释放胆碱的作用的磷脂酶D包括（a）至（c）中描述的多肽之一：（ a）含有由特定序列组成的氨基酸序列的多肽; （b）含有氨基酸序列的多肽，其中一个或多个氨基酸被取代，插入，缺失和/或添加在由特定序列组成的氨基酸序列中并具有水解活性; 和（c）与由特定序列构成的氨基酸序列具有至少90％的同源性且具有水解活性的多肽。

4. 发明专利

JP2011010609A Phospholipase (a) and method for producing 1-lysophospholipid or 2-lysophospholipid 审中-公开
标题翻译：磷脂酰胆碱（A）及其生产方法1-羟基磷脂酰胆碱磷酸酯或2-赖氨酸磷脂
公开(公告)号：JP2011010609A
公开(公告)日：2011-01-20
申请号：JP2009158274
申请日：2009-07-02
申请人： Fukushima Univ , 国立大学法人福島大学
发明人： SUGIMORI DAISUKE , NISHINA ATSURO , CHIBA YOGO
IPC分类号： C12N9/16 , A23J7/00 , C12P9/00
CPC分类号： Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide a phospholipase (A) which is high in safety and is available in food field at ease and moreover retains its activity even in neutral region, and a method for producing 1-lysophospholipid or 2-lysophospholipid using the same.SOLUTION: After a filtrate fluid in which lettuces, green onions or cucumbers are crushed to be filtered or a supernatant fluid upon centrifugation is mixed with an organic solvent such as acetone, a crude enzyme is taken out by freeze-drying or the like the formed precipitates if necessary. The crude enzyme is used as an enzyme upon decomposition of phospholipid.
摘要翻译：要解决的问题：提供安全性高的磷脂酶（A），并且易于在食品领域获得，并且即使在中性区域也保持其活性，以及使用其制备1-溶血磷脂或2-溶血磷脂的方法溶液：在将滤液，洋葱或黄瓜粉碎以过滤的过滤液或离心后的上清液与有机溶剂如丙酮混合后，通过冷冻干燥等将粗酶取出必要时形成沉淀物。粗酶在分解磷脂时用作酶。

5. 发明专利

JP2014023474A Microbial preparation for fat degradation and method for treating wastewater 有权
标题翻译：用于脂肪降解的微生物制备和处理废水的方法
公开(公告)号：JP2014023474A
公开(公告)日：2014-02-06
申请号：JP2012166341
申请日：2012-07-26
申请人： Matsumoto Biseibutsu Kenkyusho:Kk , 株式会社松本微生物研究所 , Fukushima Univ , 国立大学法人福島大学
发明人： SUGIMORI DAISUKE , MAKI TAKAAKI , SASAHIRA TAKASHI
IPC分类号： C12N1/00 , C02F3/34 , C12R1/01
摘要： PROBLEM TO BE SOLVED: To provide a microbial preparation for fat-degradation and a method for treating wastewater using a microorganism exerting an excellent fat-degrading ability at 40°C and at pH4.SOLUTION: The microbial preparation for fat-degradation contains Burkholderia multivorans. The method for wastewater treatment is characterized in that the microorganism is added to the fat-containing wastewater. The microorganism can exert excellent fat-degrading ability at a wide pH range of around 3.5-9, and/or at 30-45°C.
摘要翻译：要解决的问题：提供用于脂肪降解的微生物制剂和使用在40℃和pH4下具有优异的降脂能力的微生物处理废水的方法。解决方案：用于脂肪降解的微生物制剂包含伯克霍尔德杆菌多组分。废水处理方法的特征在于将微生物加入到含脂肪废水中。该微生物能够在3.5-9和/或30-45℃的宽pH范围内发挥优异的降脂能力。

6. 发明专利

JP2012196175A Method for producing biodiesel fuel 审中-公开
标题翻译：生物柴油燃料生产方法
公开(公告)号：JP2012196175A
公开(公告)日：2012-10-18
申请号：JP2011062253
申请日：2011-03-22
申请人： Fukushima Univ , 国立大学法人福島大学
发明人： SUGIMORI DAISUKE
IPC分类号： C12P7/64 , C10L1/02 , C10L1/08 , C11B13/00 , C11C1/06 , C11C3/08 , C12N9/16 , C12N15/09
CPC分类号： Y02E50/13 , Y02W30/74
摘要： PROBLEM TO BE SOLVED: To provide a new method for producing a biodiesel fuel.SOLUTION: The method for producing a biodiesel fuel by treating a phospholipid-containing biomass feedstock with an enzyme to hydrolize the phospholipids in the biomass feedstock along with esterifying fatty acids, is provided. In this method, as the enzyme, such one as to exhibit hydrolytic activity on the phospholipids, preferably at least either one of polypeptides each having two kinds of specific amino acid sequences, is used, wherein these polypeptides are each optionally such as to have the amino acid sequence with one or more amino acids substituted, inserted, deleted and/or added or with 75% homology. The objective biodiesel fuel can be easily produced in high productivity by subjecting the biomass feedstock to enzyme treatment.
摘要翻译：要解决的问题：提供生产生物柴油燃料的新方法。提供了通过用酶处理含磷脂生物质原料来生产生物柴油燃料的方法，以使生物质原料中的磷脂与酯化脂肪酸一起水解。在该方法中，作为对磷脂具有水解活性的酶，优选使用各自具有两种特定氨基酸序列的多肽的至少任一种，其中，这些多肽各自任选具有具有一个或多个被取代，插入，缺失和/或添加或具有75％同源性的氨基酸的氨基酸序列。通过对生物质原料进行酶处理，可以高生产率容易地生产目标生物柴油燃料。版权所有（C）2013，JPO＆INPIT

7. 发明专利

JP2011010610A Phospholipase d 审中-公开
标题翻译：磷脂D
公开(公告)号：JP2011010610A
公开(公告)日：2011-01-20
申请号：JP2009158275
申请日：2009-07-02
申请人： Fukushima Univ , 国立大学法人福島大学
发明人： SUGIMORI DAISUKE , NISHINA ATSURO , CHIBA YOGO
IPC分类号： C12N9/16
摘要： PROBLEM TO BE SOLVED: To provide a phospholipase D which is high in safety and is available in food field at ease and moreover retains high hydrolysis activity or polar group exchanging activity.SOLUTION: After a filtrate fluid in which peaches or eggplants are crushed to be filtered or a supernatant fluid upon centrifugation is mixed with an organic solvent such as acetone, a crude enzyme is taken out by freeze-drying or the like he formed precipitates if necessary. For example, when phosphatidylserine is produced using phosphatidylcholine as a raw material, the crude enzyme is used as an enzyme.
摘要翻译：要解决的问题：提供安全性高的磷脂酶D，并且易于在食品领域获得，并且还保持高水解活性或极性基团交换活性。解决方案：将桃子或茄子粉碎后的滤液过滤或离心后的上清液与有机溶剂如丙酮混合，粗酶通过冷冻干燥等取出，如果需要，它形成沉淀物。例如，当使用磷脂酰胆碱作为原料生产磷脂酰丝氨酸时，将粗酶用作酶。

8. 发明专利

JP2014027908A Method for producing biodiesel fuel 审中-公开
标题翻译：生物柴油燃料生产方法
公开(公告)号：JP2014027908A
公开(公告)日：2014-02-13
申请号：JP2012170335
申请日：2012-07-31
申请人： Fukushima Univ , 国立大学法人福島大学
发明人： SUGIMORI DAISUKE
IPC分类号： C12P7/62 , B09B3/00 , C02F11/02 , C10L1/02 , C11B11/00 , C11B13/00 , C11C1/06 , C11C3/08 , C12N9/16
CPC分类号： Y02E50/13 , Y02W30/74
摘要： PROBLEM TO BE SOLVED: To provide a new method for producing a biodiesel fuel.SOLUTION: The method for producing a biodiesel fuel is provided by treating a phospholipid-containing biomass feedstock with an enzyme to esterify fatty acids FA to fatty acid esters ME along with hydrolyzing of the phospholipids in the biomass feedstock. As the enzyme, the enzyme exhibiting hydrolytic activity on the phospholipids, preferably at least one of the enzymes which contain polypeptides each having two kinds of specific amino acid sequences, is used. These polypeptides may optionally have the amino acid sequence with one or more amino acids substituted, inserted, deleted, and/or added, or with at least 75% homology. The biodiesel fuel can be easily produced in high productivity by subjecting the biomass feedstock to enzyme treatment.
摘要翻译：要解决的问题：提供生产生物柴油燃料的新方法。解决方案：通过用酶处理含磷脂的生物质原料来提供生物柴油燃料的生产方法，以将脂肪酸FA酯化成脂肪酸酯ME以及水解生物质原料中的磷脂。作为酶，使用对磷脂表现出水解活性的酶，优选含有各自含有两种特定氨基酸序列的多肽的至少一种酶。这些多肽可任选具有一个或多个氨基酸取代，插入，缺失和/或添加或具有至少75％同源性的氨基酸序列。通过对生物质原料进行酶处理，可以容易地以高生产率生产生物柴油燃料。

9. 发明专利

JP2012105635A Microorganism preparation and method for treating waste liquid 有权
标题翻译：微生物制备和处理废液的方法
公开(公告)号：JP2012105635A
公开(公告)日：2012-06-07
申请号：JP2011193155
申请日：2011-09-05
申请人： Fukushima Univ , Matsumoto Biseibutsu Kenkyusho:Kk , 国立大学法人福島大学 , 株式会社松本微生物研究所
发明人： SUGIMORI DAISUKE , MAKI TAKAAKI , SASAHIRA TAKASHI
IPC分类号： C12N1/20 , C02F3/34 , C12N1/00 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a microorganism preparation using microorganisms capable of exhibiting excellent oil and fat-degrading ability even under a low-pH condition, and to provide a method for treating a waste liquid.SOLUTION: The microorganism preparation for the oil and fat degradation contains Raoultella planticola. The method for treating the waste liquid includes degrading the oil and fat in the waste liquid by adding the microorganisms to the waste liquid containing the oil and fat. The microorganisms can exhibit the oil and fat-degrading ability in a wide pH range of from about 3 to about 9.

10. 发明专利

JP2011010608A Phospholipase c and method for decomposing phospholipid using the same 审中-公开
标题翻译：磷脂酶C和使用它们分解磷脂的方法
公开(公告)号：JP2011010608A
公开(公告)日：2011-01-20
申请号：JP2009158273
申请日：2009-07-02
申请人： Fukushima Univ , 国立大学法人福島大学
发明人： SUGIMORI DAISUKE , NISHINA ATSURO , CHIBA YOGO
IPC分类号： C12N9/16 , C12P7/64 , C12P13/00
CPC分类号： Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide a phospholipase C which is high in safety and is available in food field at ease and moreover retains its activity even in neutral region, and a method for decomposing phospholipids using the same.SOLUTION: After a filtrate fluid in which peaches, lettuces, tomatoes, cabbages, eggplants or cucumbers are crushed to be filtered or a supernatant fluid upon centrifugation is mixed with an organic solvent such as acetone, a crude enzyme is taken out by freeze-drying or the like the formed precipitates if necessary. The crude enzyme is used as an enzyme upon decomposition of phospholipid.
摘要翻译：要解决的问题：提供安全性高的食品领域的磷脂酶C，并且即使在中性区域也可以保持其活性，并且使用该磷脂酶分解磷脂的方法。解决方案：将滤液将桃子，莴苣，西红柿，卷心菜，茄子或黄瓜粉碎以过滤或离心时的上清液与丙酮等有机溶剂混合，通过冷冻干燥等将粗酶取出形成的沉淀物，如果必要。粗酶在分解磷脂时用作酶。

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