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1. 发明专利

JP2011010608A Phospholipase c and method for decomposing phospholipid using the same 审中-公开
标题翻译：磷脂酶C和使用它们分解磷脂的方法
公开(公告)号：JP2011010608A
公开(公告)日：2011-01-20
申请号：JP2009158273
申请日：2009-07-02
申请人： Fukushima Univ , 国立大学法人福島大学
发明人： SUGIMORI DAISUKE , NISHINA ATSURO , CHIBA YOGO
IPC分类号： C12N9/16 , C12P7/64 , C12P13/00
CPC分类号： Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide a phospholipase C which is high in safety and is available in food field at ease and moreover retains its activity even in neutral region, and a method for decomposing phospholipids using the same.SOLUTION: After a filtrate fluid in which peaches, lettuces, tomatoes, cabbages, eggplants or cucumbers are crushed to be filtered or a supernatant fluid upon centrifugation is mixed with an organic solvent such as acetone, a crude enzyme is taken out by freeze-drying or the like the formed precipitates if necessary. The crude enzyme is used as an enzyme upon decomposition of phospholipid.
摘要翻译：要解决的问题：提供安全性高的食品领域的磷脂酶C，并且即使在中性区域也可以保持其活性，并且使用该磷脂酶分解磷脂的方法。解决方案：将滤液将桃子，莴苣，西红柿，卷心菜，茄子或黄瓜粉碎以过滤或离心时的上清液与丙酮等有机溶剂混合，通过冷冻干燥等将粗酶取出形成的沉淀物，如果必要。粗酶在分解磷脂时用作酶。

2. 发明专利

JP2011010610A Phospholipase d 审中-公开
标题翻译：磷脂D
公开(公告)号：JP2011010610A
公开(公告)日：2011-01-20
申请号：JP2009158275
申请日：2009-07-02
申请人： Fukushima Univ , 国立大学法人福島大学
发明人： SUGIMORI DAISUKE , NISHINA ATSURO , CHIBA YOGO
IPC分类号： C12N9/16
摘要： PROBLEM TO BE SOLVED: To provide a phospholipase D which is high in safety and is available in food field at ease and moreover retains high hydrolysis activity or polar group exchanging activity.SOLUTION: After a filtrate fluid in which peaches or eggplants are crushed to be filtered or a supernatant fluid upon centrifugation is mixed with an organic solvent such as acetone, a crude enzyme is taken out by freeze-drying or the like he formed precipitates if necessary. For example, when phosphatidylserine is produced using phosphatidylcholine as a raw material, the crude enzyme is used as an enzyme.
摘要翻译：要解决的问题：提供安全性高的磷脂酶D，并且易于在食品领域获得，并且还保持高水解活性或极性基团交换活性。解决方案：将桃子或茄子粉碎后的滤液过滤或离心后的上清液与有机溶剂如丙酮混合，粗酶通过冷冻干燥等取出，如果需要，它形成沉淀物。例如，当使用磷脂酰胆碱作为原料生产磷脂酰丝氨酸时，将粗酶用作酶。

3. 发明专利

JP2011010609A Phospholipase (a) and method for producing 1-lysophospholipid or 2-lysophospholipid 审中-公开
标题翻译：磷脂酰胆碱（A）及其生产方法1-羟基磷脂酰胆碱磷酸酯或2-赖氨酸磷脂
公开(公告)号：JP2011010609A
公开(公告)日：2011-01-20
申请号：JP2009158274
申请日：2009-07-02
申请人： Fukushima Univ , 国立大学法人福島大学
发明人： SUGIMORI DAISUKE , NISHINA ATSURO , CHIBA YOGO
IPC分类号： C12N9/16 , A23J7/00 , C12P9/00
CPC分类号： Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide a phospholipase (A) which is high in safety and is available in food field at ease and moreover retains its activity even in neutral region, and a method for producing 1-lysophospholipid or 2-lysophospholipid using the same.SOLUTION: After a filtrate fluid in which lettuces, green onions or cucumbers are crushed to be filtered or a supernatant fluid upon centrifugation is mixed with an organic solvent such as acetone, a crude enzyme is taken out by freeze-drying or the like the formed precipitates if necessary. The crude enzyme is used as an enzyme upon decomposition of phospholipid.
摘要翻译：要解决的问题：提供安全性高的磷脂酶（A），并且易于在食品领域获得，并且即使在中性区域也保持其活性，以及使用其制备1-溶血磷脂或2-溶血磷脂的方法溶液：在将滤液，洋葱或黄瓜粉碎以过滤的过滤液或离心后的上清液与有机溶剂如丙酮混合后，通过冷冻干燥等将粗酶取出必要时形成沉淀物。粗酶在分解磷脂时用作酶。

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