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1. 发明专利

JP2012105635A Microorganism preparation and method for treating waste liquid 有权
标题翻译：微生物制备和处理废液的方法
公开(公告)号：JP2012105635A
公开(公告)日：2012-06-07
申请号：JP2011193155
申请日：2011-09-05
申请人： Fukushima Univ , Matsumoto Biseibutsu Kenkyusho:Kk , 国立大学法人福島大学 , 株式会社松本微生物研究所
发明人： SUGIMORI DAISUKE , MAKI TAKAAKI , SASAHIRA TAKASHI
IPC分类号： C12N1/20 , C02F3/34 , C12N1/00 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a microorganism preparation using microorganisms capable of exhibiting excellent oil and fat-degrading ability even under a low-pH condition, and to provide a method for treating a waste liquid.SOLUTION: The microorganism preparation for the oil and fat degradation contains Raoultella planticola. The method for treating the waste liquid includes degrading the oil and fat in the waste liquid by adding the microorganisms to the waste liquid containing the oil and fat. The microorganisms can exhibit the oil and fat-degrading ability in a wide pH range of from about 3 to about 9.
摘要翻译：待解决的问题：即使在低pH条件下也能够使用能够表现出优异的油脂降解能力的微生物提供微生物制剂，并且提供了处理废液的方法。解决方案：油和脂肪降解的微生物制剂含有蓖麻（Raoultella planticola）。处理废液的方法包括通过将微生物添加到含有油脂的废液中来降解废液中的油脂。微生物可以在约3至约9的宽pH范围内显示出油和脂肪降解能力。版权所有（C）2012，JPO＆INPIT

2. 发明专利

JP2014023474A Microbial preparation for fat degradation and method for treating wastewater 有权
标题翻译：用于脂肪降解的微生物制备和处理废水的方法
公开(公告)号：JP2014023474A
公开(公告)日：2014-02-06
申请号：JP2012166341
申请日：2012-07-26
申请人： Matsumoto Biseibutsu Kenkyusho:Kk , 株式会社松本微生物研究所 , Fukushima Univ , 国立大学法人福島大学
发明人： SUGIMORI DAISUKE , MAKI TAKAAKI , SASAHIRA TAKASHI
IPC分类号： C12N1/00 , C02F3/34 , C12R1/01
摘要： PROBLEM TO BE SOLVED: To provide a microbial preparation for fat-degradation and a method for treating wastewater using a microorganism exerting an excellent fat-degrading ability at 40°C and at pH4.SOLUTION: The microbial preparation for fat-degradation contains Burkholderia multivorans. The method for wastewater treatment is characterized in that the microorganism is added to the fat-containing wastewater. The microorganism can exert excellent fat-degrading ability at a wide pH range of around 3.5-9, and/or at 30-45°C.
摘要翻译：要解决的问题：提供用于脂肪降解的微生物制剂和使用在40℃和pH4下具有优异的降脂能力的微生物处理废水的方法。解决方案：用于脂肪降解的微生物制剂包含伯克霍尔德杆菌多组分。废水处理方法的特征在于将微生物加入到含脂肪废水中。该微生物能够在3.5-9和/或30-45℃的宽pH范围内发挥优异的降脂能力。

3. 发明专利

JP2014082991A Phospholipase d, polynucleotide, and production method of phospholipase d 有权
标题翻译：磷脂酶D，多核苷酸和磷脂酶D的生产方法
公开(公告)号：JP2014082991A
公开(公告)日：2014-05-12
申请号：JP2012234402
申请日：2012-10-24
申请人： Fukushima Univ , 国立大学法人福島大学
发明人： SUGIMORI DAISUKE , MATSUMOTO YUSAKU
IPC分类号： C12N15/09 , C12N9/16
摘要： PROBLEM TO BE SOLVED: To provide a phospholipase D which can act on a cholinergic plasmalogen, and can act on a lysophosphatidylcholine to inexpensively produce a lysophosphatidic acid, and a polynucleotide, which are obtained from a microorganism easily handled, and to provide a production method of the phospholipase D.SOLUTION: A phospholipase D which exerts an action in which a cholinergic plasmalogen and a tertiary phosphoric ester of a lysophospholipid are hydrolyzed to liberate a choline, includes either one of polypeptides described in (a) to (c): (a) a polypeptide containing an amino acid sequence consisting of a specific sequence; (b) a polypeptide containing an amino acid sequence in which one or a plurality of amino acids are substituted, inserted, deleted and/or added in the amino acid sequence consisting of the specific sequence, and having hydrolytic activity; and (c) a polypeptide containing at least 90% of homology with the amino acid sequence consisting of the specific sequence, and having hydrolytic activity.
摘要翻译：要解决的问题：提供可以作用于胆碱能浆细胞原子的磷脂酶D，并且可以作用于溶血磷脂酰胆碱以便廉价地产生溶血磷脂酸，以及从易于处理的微生物获得的多核苷酸，并提供生产方法的磷脂酶D。解决方案：发挥其中胆碱能浆果原和溶血磷脂的叔磷酸酯水解以释放胆碱的作用的磷脂酶D包括（a）至（c）中描述的多肽之一：（ a）含有由特定序列组成的氨基酸序列的多肽; （b）含有氨基酸序列的多肽，其中一个或多个氨基酸被取代，插入，缺失和/或添加在由特定序列组成的氨基酸序列中并具有水解活性; 和（c）与由特定序列构成的氨基酸序列具有至少90％的同源性且具有水解活性的多肽。

4. 发明专利

JP2011010609A Phospholipase (a) and method for producing 1-lysophospholipid or 2-lysophospholipid 审中-公开
标题翻译：磷脂酰胆碱（A）及其生产方法1-羟基磷脂酰胆碱磷酸酯或2-赖氨酸磷脂
公开(公告)号：JP2011010609A
公开(公告)日：2011-01-20
申请号：JP2009158274
申请日：2009-07-02
申请人： Fukushima Univ , 国立大学法人福島大学
发明人： SUGIMORI DAISUKE , NISHINA ATSURO , CHIBA YOGO
IPC分类号： C12N9/16 , A23J7/00 , C12P9/00
CPC分类号： Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide a phospholipase (A) which is high in safety and is available in food field at ease and moreover retains its activity even in neutral region, and a method for producing 1-lysophospholipid or 2-lysophospholipid using the same.SOLUTION: After a filtrate fluid in which lettuces, green onions or cucumbers are crushed to be filtered or a supernatant fluid upon centrifugation is mixed with an organic solvent such as acetone, a crude enzyme is taken out by freeze-drying or the like the formed precipitates if necessary. The crude enzyme is used as an enzyme upon decomposition of phospholipid.
摘要翻译：要解决的问题：提供安全性高的磷脂酶（A），并且易于在食品领域获得，并且即使在中性区域也保持其活性，以及使用其制备1-溶血磷脂或2-溶血磷脂的方法溶液：在将滤液，洋葱或黄瓜粉碎以过滤的过滤液或离心后的上清液与有机溶剂如丙酮混合后，通过冷冻干燥等将粗酶取出必要时形成沉淀物。粗酶在分解磷脂时用作酶。

5. 发明专利

JP2012196175A Method for producing biodiesel fuel 审中-公开
标题翻译：生物柴油燃料生产方法
公开(公告)号：JP2012196175A
公开(公告)日：2012-10-18
申请号：JP2011062253
申请日：2011-03-22
申请人： Fukushima Univ , 国立大学法人福島大学
发明人： SUGIMORI DAISUKE
IPC分类号： C12P7/64 , C10L1/02 , C10L1/08 , C11B13/00 , C11C1/06 , C11C3/08 , C12N9/16 , C12N15/09
CPC分类号： Y02E50/13 , Y02W30/74
摘要： PROBLEM TO BE SOLVED: To provide a new method for producing a biodiesel fuel.SOLUTION: The method for producing a biodiesel fuel by treating a phospholipid-containing biomass feedstock with an enzyme to hydrolize the phospholipids in the biomass feedstock along with esterifying fatty acids, is provided. In this method, as the enzyme, such one as to exhibit hydrolytic activity on the phospholipids, preferably at least either one of polypeptides each having two kinds of specific amino acid sequences, is used, wherein these polypeptides are each optionally such as to have the amino acid sequence with one or more amino acids substituted, inserted, deleted and/or added or with 75% homology. The objective biodiesel fuel can be easily produced in high productivity by subjecting the biomass feedstock to enzyme treatment.
摘要翻译：要解决的问题：提供生产生物柴油燃料的新方法。提供了通过用酶处理含磷脂生物质原料来生产生物柴油燃料的方法，以使生物质原料中的磷脂与酯化脂肪酸一起水解。在该方法中，作为对磷脂具有水解活性的酶，优选使用各自具有两种特定氨基酸序列的多肽的至少任一种，其中，这些多肽各自任选具有具有一个或多个被取代，插入，缺失和/或添加或具有75％同源性的氨基酸的氨基酸序列。通过对生物质原料进行酶处理，可以高生产率容易地生产目标生物柴油燃料。版权所有（C）2013，JPO＆INPIT

6. 发明专利

JP2011010610A Phospholipase d 审中-公开
标题翻译：磷脂D
公开(公告)号：JP2011010610A
公开(公告)日：2011-01-20
申请号：JP2009158275
申请日：2009-07-02
申请人： Fukushima Univ , 国立大学法人福島大学
发明人： SUGIMORI DAISUKE , NISHINA ATSURO , CHIBA YOGO
IPC分类号： C12N9/16
摘要： PROBLEM TO BE SOLVED: To provide a phospholipase D which is high in safety and is available in food field at ease and moreover retains high hydrolysis activity or polar group exchanging activity.SOLUTION: After a filtrate fluid in which peaches or eggplants are crushed to be filtered or a supernatant fluid upon centrifugation is mixed with an organic solvent such as acetone, a crude enzyme is taken out by freeze-drying or the like he formed precipitates if necessary. For example, when phosphatidylserine is produced using phosphatidylcholine as a raw material, the crude enzyme is used as an enzyme.
摘要翻译：要解决的问题：提供安全性高的磷脂酶D，并且易于在食品领域获得，并且还保持高水解活性或极性基团交换活性。解决方案：将桃子或茄子粉碎后的滤液过滤或离心后的上清液与有机溶剂如丙酮混合，粗酶通过冷冻干燥等取出，如果需要，它形成沉淀物。例如，当使用磷脂酰胆碱作为原料生产磷脂酰丝氨酸时，将粗酶用作酶。

7. 发明专利

JP2014027908A Method for producing biodiesel fuel 审中-公开
标题翻译：生物柴油燃料生产方法
公开(公告)号：JP2014027908A
公开(公告)日：2014-02-13
申请号：JP2012170335
申请日：2012-07-31
申请人： Fukushima Univ , 国立大学法人福島大学
发明人： SUGIMORI DAISUKE
IPC分类号： C12P7/62 , B09B3/00 , C02F11/02 , C10L1/02 , C11B11/00 , C11B13/00 , C11C1/06 , C11C3/08 , C12N9/16
CPC分类号： Y02E50/13 , Y02W30/74
摘要： PROBLEM TO BE SOLVED: To provide a new method for producing a biodiesel fuel.SOLUTION: The method for producing a biodiesel fuel is provided by treating a phospholipid-containing biomass feedstock with an enzyme to esterify fatty acids FA to fatty acid esters ME along with hydrolyzing of the phospholipids in the biomass feedstock. As the enzyme, the enzyme exhibiting hydrolytic activity on the phospholipids, preferably at least one of the enzymes which contain polypeptides each having two kinds of specific amino acid sequences, is used. These polypeptides may optionally have the amino acid sequence with one or more amino acids substituted, inserted, deleted, and/or added, or with at least 75% homology. The biodiesel fuel can be easily produced in high productivity by subjecting the biomass feedstock to enzyme treatment.
摘要翻译：要解决的问题：提供生产生物柴油燃料的新方法。解决方案：通过用酶处理含磷脂的生物质原料来提供生物柴油燃料的生产方法，以将脂肪酸FA酯化成脂肪酸酯ME以及水解生物质原料中的磷脂。作为酶，使用对磷脂表现出水解活性的酶，优选含有各自含有两种特定氨基酸序列的多肽的至少一种酶。这些多肽可任选具有一个或多个氨基酸取代，插入，缺失和/或添加或具有至少75％同源性的氨基酸序列。通过对生物质原料进行酶处理，可以容易地以高生产率生产生物柴油燃料。

8. 发明专利

JP2011010608A Phospholipase c and method for decomposing phospholipid using the same 审中-公开
标题翻译：磷脂酶C和使用它们分解磷脂的方法
公开(公告)号：JP2011010608A
公开(公告)日：2011-01-20
申请号：JP2009158273
申请日：2009-07-02
申请人： Fukushima Univ , 国立大学法人福島大学
发明人： SUGIMORI DAISUKE , NISHINA ATSURO , CHIBA YOGO
IPC分类号： C12N9/16 , C12P7/64 , C12P13/00
CPC分类号： Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide a phospholipase C which is high in safety and is available in food field at ease and moreover retains its activity even in neutral region, and a method for decomposing phospholipids using the same.SOLUTION: After a filtrate fluid in which peaches, lettuces, tomatoes, cabbages, eggplants or cucumbers are crushed to be filtered or a supernatant fluid upon centrifugation is mixed with an organic solvent such as acetone, a crude enzyme is taken out by freeze-drying or the like the formed precipitates if necessary. The crude enzyme is used as an enzyme upon decomposition of phospholipid.
摘要翻译：要解决的问题：提供安全性高的食品领域的磷脂酶C，并且即使在中性区域也可以保持其活性，并且使用该磷脂酶分解磷脂的方法。解决方案：将滤液将桃子，莴苣，西红柿，卷心菜，茄子或黄瓜粉碎以过滤或离心时的上清液与丙酮等有机溶剂混合，通过冷冻干燥等将粗酶取出形成的沉淀物，如果必要。粗酶在分解磷脂时用作酶。

9. 发明专利

JP2010178748A Method for treating oil and fat-containing substance 有权
标题翻译：用于处理油和含脂物质的方法
公开(公告)号：JP2010178748A
公开(公告)日：2010-08-19
申请号：JP2010065259
申请日：2010-03-19
申请人： Fukushima Univ , Sakura Nyugyo Kk , 国立大学法人福島大学 , 桜乳業株式会社
发明人： SUGIMORI DAISUKE , SAKURAOKA TOSHIYUKI
IPC分类号： C12N1/16 , C02F3/34
摘要： PROBLEM TO BE SOLVED: To provide a method for treating oil and fat-containing substances using a microorganism having a high oil and fat degrading effect. SOLUTION: This method for treating the oil and fat-containing substances comprises degrading the oil and fat with Rhodotorula pacifica. Even when the contents of nitrogen and phosphorus are small, and even when a grease trap has a low temperature of 20 to 25°C which is the average water temperature, an oil and fat degradation rate can remarkably be improved. Since good results are obtained for various kinds of fats and oils, the method can widely be applied. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：提供一种使用具有高油脂降解效果的微生物处理含油脂物质的方法。解决方案：用于处理含油和脂肪的物质的方法包括用红薯属（Rhodotorula pacifica）降解油脂。即使当氮和磷的含量较小时，即使在油水分离器的温度低于平均水温的20〜25℃的情况下，也可以显着提高油脂的降解率。由于可以获得各种脂肪和油的良好结果，所以该方法可以广泛应用。版权所有（C）2010，JPO＆INPIT

10. 发明专利

JP2014233219A エタノールアミンオキシダーゼ有权
标题翻译：乙醇胺氧化酶
公开(公告)号：JP2014233219A
公开(公告)日：2014-12-15
申请号：JP2013115395
申请日：2013-05-31
申请人：国立大学法人福島大学 , Fukushima Univ , 旭化成ファーマ株式会社 , Asahi Kasei Pharma Kk
发明人： SUGIMORI DAISUKE , SAKASEGAWA SHINICHI , MATSUMOTO HIDEYUKI
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/06 , C12P3/00 , C12P7/24
CPC分类号： Y02P20/52
摘要：【課題】モノエタノールアミンを効率良く酸化する作用を有する蛋白質を提供する。【解決手段】下記(A)又は(B)の蛋白質:(A)特定のアミノ酸配列からなる蛋白質;(B)特定のアミノ酸配列において、1又は複数個のアミノ酸が欠失、置換若しくは付加されたアミノ酸配列からなり、式:HOCH2CH2NH2+O2+H2O→HOCH2CHO+NH3+H2O2で表される反応を触媒する蛋白質。前記塩基配列を含む遺伝子。前記遺伝子を含む組換えベクター。前記組換えベクターを含む形質転換体。前記蛋白質の製造方法。前記蛋白質を用いるモノエタノールアミンの酸化方法。前記蛋白質を用いてモノエタノールアミンからグリコールアルデヒド、アンモニア、又は過酸化水素を製造する方法。前記蛋白質を含有するモノエタノールアミン酸化剤。【選択図】なし
摘要翻译：要解决的问题：提供能够有效地氧化单乙醇胺的蛋白质。解决方案：本发明提供以下（A）或（B）的蛋白质：（A）特定氨基酸序列的蛋白质; （B）其氨基酸序列与一个或多个缺失，取代或加入的氨基酸的氨基酸序列不同的蛋白质，并催化由式HOCHCHNH + O + HO→HOCHCHO + NH + HO表示的反应。本发明还提供了包含编码该蛋白质的碱基序列的基因，包含该基因的重组载体，包含该重组载体的转化体，该蛋白质的制备方法，使用该蛋白质来氧化单乙醇胺的方法，，氨或过氧化氢，和包含该蛋白质的单乙醇胺氧化剂。

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