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1. 发明专利

JP2004267099A Method for detecting escherichia coli and phage for detecting escherichia coli 有权
标题翻译：用于检测ESCHERICHIA COLI和PHAGE的检测方法
公开(公告)号：JP2004267099A
公开(公告)日：2004-09-30
申请号：JP2003062440
申请日：2003-03-07
申请人： Ebara Corp , Rikogaku Shinkokai , 株式会社荏原製作所 , 財団法人理工学振興会
发明人： TANJI YASUNORI , UNNO HAJIME , FURUKAWA CHIAKI , ONDA KENSUKE , MIYA AKIKO , KENMOCHI YUKIO
IPC分类号： C12N15/09 , C07K14/01 , C12N7/00 , C12N7/01 , C12Q1/02 , C12Q1/06 , C12Q1/10 , C12Q1/68 , C12Q1/70
CPC分类号： C12N7/00 , C07K14/005 , C07K2319/60 , C12N2795/10122 , C12Q1/10 , C12Q1/689
摘要： PROBLEM TO BE SOLVED: To provide a method for detecting Escherichia coli with extremely improved sensitivity by utilizing a phage by which the decrease of sensitivity caused by bacteriolysis is reduced, and the live bacteria can be specifically detected. SOLUTION: The method for detecting the Escherichia coli involves detecting the Escherichia coli present in a specimen by bringing the phage infecting specified Escherichia coli into contact with the specimen. The method comprises using the phage the coat of which has a fluorescent protein expressed therein as a protein fused with the coat protein, and which has defects in a lytic enzyme. Particulary, the method comprises detecting the fluorescence at an intensity level emitted from the fluorescent protein of the phage adsorbed on the Escherichia coli, and/or detecting the fluorescence at the intensity level emitted from the fluorescent protein expressed in the Escherichia coli caused by the adsorption of the phage. COPYRIGHT: (C)2004,JPO&NCIPI
摘要翻译：要解决的问题：提供一种通过利用由细菌溶解引起的敏感性降低降低的噬菌体来检测具有极高的灵敏度的大肠杆菌的方法，并且可以特异性地检测活细菌。解决方案：用于检测大肠杆菌的方法包括通过使感染指定的大肠杆菌的噬菌体与样品接触来检测样品中存在的大肠杆菌。该方法包括使用其外含有其中表达的荧光蛋白的噬菌体作为与外壳蛋白融合的蛋白质，并且在裂解酶中具有缺陷。所述方法包括从吸附在大肠杆菌上的噬菌体的荧光蛋白发射的强度水平检测荧光，和/或从由吸附引起的大肠杆菌中表达的荧光蛋白发射的强度水平检测荧光的噬菌体。版权所有（C）2004，JPO＆NCIPI

2. 发明专利

JP2007289946A Anaerobic treatment process and device of organic solid waste 审中-公开
标题翻译：有机固体废物处理工艺及装置
公开(公告)号：JP2007289946A
公开(公告)日：2007-11-08
申请号：JP2007090092
申请日：2007-03-30
申请人： Ebara Corp , 株式会社荏原製作所
发明人： TATEZAWA TOMOKO , HAO RINYUN , MIYA AKIKO
IPC分类号： B09B3/00 , B01F7/00 , C02F11/04
CPC分类号： Y02E50/343
摘要： PROBLEM TO BE SOLVED: To provide an anaerobic treatment device capable of enhancing a conversion ratio to methane and reducing the volume of excess sludge by simply efficiently making an organic solid soluble and low-molecular, and a process. SOLUTION: The anaerobic treatment device of waste 1 containing the organic solid comprises a first anaerobic reactor 2 for carrying out solubilization and acid fermentation under an anaerobic condition, a second anaerobic reactor 5 for carrying out methane fermentation under the anaerobic condition, and a liquid feed device for feeding a liquid from the top of the first anaerobic reactor 2 to the second anaerobic reactor 5 while keeping the anaerobic condition, wherein the first anaerobic reactor 2 includes an agitation device for agitating a material to be treated and a control mechanism of forming a solid concentration distribution concentrating the material to be treated in a vertical direction from the top to the bottom in the first anaerobic reactor 2 by controlling the agitation device and wherein the first anaerobic reactor 2 has a tapered shape gradually narrow from the top to the bottom, arranges a solid-liquid separation device at the rearmost stage of the second anaerobic reactor 5 and includes a recirculation pipe for introducing a part of the material to be treated concentrating the obtained solid into the first anaerobic reactor 2. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：要解决的问题：提供一种厌氧处理装置，其能够通过简单有效地制备有机固体可溶性和低分子量以及一种方法来提高甲烷的转化率和减少过量污泥的体积。解决方案：含有机固体的废物1的厌氧处理装置包括：在厌氧条件下进行增溶和酸发酵的第一厌氧反应器2，用于在厌氧条件下进行甲烷发酵的第二厌氧反应器5，以及用于在保持厌氧条件的同时将液体从第一厌氧反应器2的顶部供给到第二厌氧反应器5的液体进料装置，其中第一厌氧反应器2包括用于搅拌待处理材料的搅拌装置和控制机构通过控制所述搅拌装置，在所述第一厌氧反应器2中形成从所述顶部至底部的垂直方向上浓缩所述处理材料的固体浓度分布，并且其中所述第一厌氧反应器2具有从顶部逐渐变窄的渐缩形状，底部，在第二个厌氧的最后阶段安排一个固液分离装置反应器5，并且包括用于将待处理材料的一部分引入的再循环管道，将获得的固体浓缩到第一厌氧反应器2中。（C）2008年，JPO和INPIT

3. 发明专利

JP2007090232A Method and device for treating organic substance-containing waste solution 审中-公开
标题翻译：用于处理含有有机物质的废物溶液的方法和装置
公开(公告)号：JP2007090232A
公开(公告)日：2007-04-12
申请号：JP2005282775
申请日：2005-09-28
申请人： Ebara Corp , 株式会社荏原製作所
发明人： ADACHI MASANORI , MIYA AKIKO , SHIMOMURA TATSUO
IPC分类号： C02F3/30 , C02F1/28 , C02F1/32 , C02F1/52 , C02F1/58 , C02F1/72 , C02F1/78 , C02F3/04 , C02F3/06 , C02F3/34 , C02F11/04 , H01M8/16
CPC分类号： Y02E60/527 , Y02W10/15
摘要： PROBLEM TO BE SOLVED: To provide a method and a device for treating organic substance by utilizing a biological power generation technique capable of stably achiving SOLUTION: The organic substance-containing waste solution 1 is fed from a raw water storage tank 2 to the anaerobic region 5a in a biological power generator 5. On the other hand, oxygen or oxygen-containing air humidified so as to be a relative humidity of 100% is fed to the aerobic region 5b in the biological power generator 5. At this time, the oxygen or oxygen-containing air may be circulated through the aerobic region 5b in the biological power generator 5 using a pump or a fan, or may be circulated utilizing thermal convection. The organic substance-containing waste solution 1 is passed through the anaerobic region 5a while retaining its pH to a suitable range by a pH controller 8, and oxidation reaction by microorganisms having electrode activity and reduction reaction by oxygen are progressed, thus water treatment is performed simultaneously with power generation. A treated solution 6 is fed to a posttreatment tank 10 from the exhaust port of the anaerobic region 5a in the biological generator 5, so as to obtain secondarily treated water 11. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：提供一种利用生物发电技术来提供治疗有机物质的方法和装置，所述生物发电技术能够稳定地将120mg / L的BOD（生物需氧量）定为均匀的排出物标准（日平均值）基于“水污染控制法”。解决方案：将含有机物的废液1从原水储存罐2供给到生物发电机5中的厌氧区5a。另一方面，将含氧气体或含氧空气加湿至 100％的相对湿度被供给到生物发生器5中的需氧区域5b中。此时，氧气或含氧空气可以通过使用泵或者氧气的生物发生器5中的需氧区域循环风扇，或可以利用热对流循环。含有有机物的废液1通过pH值控制器8将pH值保持在合适的范围内，通过厌氧区域5a进行，并且进行具有电极活性的微生物的氧化反应和氧的还原反应，进行水处理同时发电。处理溶液6从生物发生器5中的厌氧区域5a的排气口供给到后处理槽10，以获得二次处理的水11.版权所有（C）2007，JPO＆INPIT

4. 发明专利

JP2006034171A Bisphenol a-degrading microorganism and application thereof 有权
标题翻译：双酚A降解微生物及其应用
公开(公告)号：JP2006034171A
公开(公告)日：2006-02-09
申请号：JP2004218947
申请日：2004-07-27
申请人： Ebara Corp , Japan Sewage Works Agency , 日本下水道事業団 , 株式会社荏原製作所
发明人： ONDA KENSUKE , MORITA TOMOYUKI , MIYA AKIKO , TADA KEITARO , HASHIMOTO TOSHIICHI , MISHINA FUMIO
IPC分类号： C12N1/20 , C02F3/34 , C02F11/02 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide microorganisms capable of efficiently degrading bisphenol A; and to provide a degrading and treating method or the like, capable of efficiently degrading and treating the bisphenol A contained in a waste water, a waste material or the like by using the microorganisms.
SOLUTION: The bisphenol A-degrading microorganism has abilities for degrading the bisphenol A and belongs to the genus Nocardioides. The representative strain is Nocardioides sp. JBB-41 strain (FERM AP-20004). The method for degrading and treating the bisphenol A involves a step for bringing the bisphenol A-degrading microorganisms into contact with a sample from which the bisphenol A is to be removed.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供能够有效降解双酚A的微生物; 并且提供能够通过使用微生物有效降解和处理包含在废水，废物等中的双酚A的降解和处理方法等。解决方案：双酚A降解微生物具有降解双酚A并属于诺卡氏菌属的能力。代表性菌株是诺卡氏菌属（Nocardioides sp。 JBB-41菌株（FERM AP-20004）。降解和处理双酚A的方法包括使双酚A降解微生物与要除去双酚A的样品接触的步骤。版权所有（C）2006，JPO＆NCIPI

5. 发明专利

JP2005125319A Method and apparatus for power generation by organic waste 有权
标题翻译：有机废物发电的方法和装置
公开(公告)号：JP2005125319A
公开(公告)日：2005-05-19
申请号：JP2004287743
申请日：2004-09-30
申请人： Ebara Corp , 株式会社荏原製作所
发明人： MIYA AKIKO , KATAOKA NAOAKI , SERIKAWA ROBERTO MASAHIRO , USUI TAKASHI , SUZUKI TAKAYUKI
IPC分类号： B09B3/00 , C02F11/00 , C02F11/04 , C02F11/08 , H01M8/06
CPC分类号： Y02E50/343
摘要： PROBLEM TO BE SOLVED: To provide an economical and extremely low environmental-load technology which produces electric energy from organic waste by using biological reactions and chemical reactions. SOLUTION: The method and the apparatus for power generation by the organic waste are characterized in that, in the power generation method by the organic waste comprising a process for producing gaseous fuel from the organic waste by the biological reactions, a gas purification process for purifying gas obtained from the gaseous fuel production process, and a power generation process for supplying the purified gas and generating power, whole or a part of waste liquid containing a residue discharged from the process for producing the gaseous fuel by the biological reactions is fed to a hydrothermal electrolysis process for supplying a direct current, at a temperature not less than 100°C and below the critical temperature of the waste liquid, under the pressure making the waste liquid maintain its liquid phase, and hydrogen gas recovered in the hydrothermal electrolysis process is used after being mixed with gas obtained from the gaseous fuel production process, or both gases are used independently. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供经济和极低的环境负荷技术，通过使用生物反应和化学反应从有机废物产生电能。解决方案：有机废物发电的方法和装置的特征在于，在有机废弃物的发电方法中，包括通过生物反应从有机废物生产气态燃料的方法，气体净化从气态燃料生产过程获得的气体净化工艺，以及用于提供净化气体和产生动力的发电工艺，包含通过生物反应从生产气态燃料的工艺排出的残余物的废液的全部或一部分是进入水热电解过程，在不低于100℃并低于废液临界温度的温度下供给直流电，在压力下使废液保持其液相，并在氢热中回收氢气在与气体燃料生产过程中获得的气体混合后使用电解过程，或者使用两种气体 d独立。版权所有（C）2005，JPO＆NCIPI

6. 发明专利

JP2004261126A Method for evaluating alkane decomposing ability of environmental specimen and oligonucleotide therefor 审中-公开
标题翻译：评估环境样品及其寡核苷酸的碱分解能力的方法
公开(公告)号：JP2004261126A
公开(公告)日：2004-09-24
申请号：JP2003056155
申请日：2003-03-03
申请人： Ebara Corp , 株式会社荏原製作所
发明人： KARUBE MAKIKO , TAMATSUBO KAZUAKI , MIYA AKIKO
IPC分类号： C12N15/09 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To evaluate the alkane decomposing ability of an environmental specimen by determining the number of bacterial cells having high alkane decomposing ability and dominant in the alkane decomposing environmental specimen. SOLUTION: The alkane decomposing ability of an environmental specimen is evaluated by determining the number of the cells of alkane decomposing bacteria existing in the environmental specimen and having the properties of (1) having high decomposing ability to at least one kind of alkane derived from petroleum and (2) having a 16SrRNA having at least 93% homology to the base sequence of 16SrRNA of Rhodococcus and/or a gyrBDNA having at least 84% homology to the base sequence of gyrB of Rhodococcus. COPYRIGHT: (C)2004,JPO&NCIPI
摘要翻译：要解决的问题：通过确定烷烃分解能力高的烷烃分解能力的细菌细胞的数量和烷烃分解环境标本中的优势，评价环境样品的烷烃分解能力。解决方案：环境样品的烷烃分解能力通过确定环境样品中存在的烷烃分解细菌的细胞数，并具有（1）具有高分解能力的性质至少一种烷烃来评估衍生自石油和（2）具有与Rhodococcus的16SrRNA的碱基序列具有至少93％同源性的16SrRNA和/或与红球菌的gyrB的碱基序列具有至少84％同源性的gyrBDNA。版权所有（C）2004，JPO＆NCIPI

7. 发明专利

JP2005253429A Method for detecting high temperature anaerobic protein-degrading bacterium and oligonucelotide for detection 审中-公开
标题翻译：检测高温厌氧蛋白降解细菌和寡核苷酸检测方法
公开(公告)号：JP2005253429A
公开(公告)日：2005-09-22
申请号：JP2004073169
申请日：2004-03-15
申请人： Ebara Corp , 株式会社荏原製作所
发明人： NAGAYA YOSHIKI , TATEZAWA TOMOKO , KARUBE MAKIKO , MIYA AKIKO , TAMATSUBO KAZUAKI
IPC分类号： C12N15/09 , B09B3/00 , C02F11/04 , C12N1/00 , C12Q1/68
CPC分类号： Y02E50/343
摘要： PROBLEM TO BE SOLVED: To provide a method for evaluating a protein-degrading ability in an environmental sample. SOLUTION: This method for evaluating a protein-degrading ability in an environmental sample, comprising measuring a protein-degrading bacterium by a quantitative PCR method using the following primer set. (a) An oligonucleotide having the first specific base sequence or its substantially homologous base sequence, and an oligonucleotide having the second specific base sequence or its substantially homologous base sequence. (b) An oligonucleotide having the third specific base sequence or its substantially homologous base sequence, and an oligonucleotide having the second specific base sequence or its substantially homologous base sequence. (c) An oligonucleotide having the fourth specific base sequence or its substantially homologous base sequence, and an oligonucleotide having the fifth specific base sequence or its substantially homologous base sequence. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：待解决的问题：提供一种评估环境样品中蛋白质降解能力的方法。解决方案：用于评估环境样品中蛋白质降解能力的方法，包括使用以下引物组通过定量PCR方法测量蛋白质降解细菌。（a）具有第一特异碱基序列或其基本上同源的碱基序列的寡核苷酸，以及具有第二特异碱基序列或其基本上同源的碱基序列的寡核苷酸。（b）具有第三特异碱基序列或其基本同源碱基序列的寡核苷酸，以及具有第二特异碱基序列或其基本上同源的碱基序列的寡核苷酸。（c）具有第四特异碱基序列或其基本上同源的碱基序列的寡核苷酸和具有第五特异碱基序列或其基本上同源的碱基序列的寡核苷酸。版权所有（C）2005，JPO＆NCIPI

8. 发明专利

JP2005253428A Nucleic acid primer for detecting female hormone-degrading microorganism and method for detecting female hormone-degrading microorganism 审中-公开
标题翻译：用于检测雌激素降解微生物的核酸原液和检测雌激素降解微生物的方法
公开(公告)号：JP2005253428A
公开(公告)日：2005-09-22
申请号：JP2004073167
申请日：2004-03-15
申请人： Ebara Corp , Japan Sewage Works Agency , 日本下水道事業団 , 株式会社荏原製作所
发明人： ONDA KENSUKE , MORITA TOMOYUKI , MIYA AKIKO , TADA KEITARO , HASHIMOTO TOSHIICHI , MISHINA FUMIO , LUXMY BEGUM SHAILA
IPC分类号： C12N15/09 , C02F3/12 , C12Q1/68
CPC分类号： Y02W10/15
摘要： PROBLEM TO BE SOLVED: To provide a method for specifically evaluating the amount of a female hormone-degrading microorganism having a female hormone-degrading ability in a short time. SOLUTION: The method for detecting the female hormone-degrading microorganism existing in activated sludge or the like comprises subjecting a nucleic acid specimen originated from the female hormone-degrading microorganism existing in the activated sludge or the like to a PCR method using primers designed on consensus regions in the 16SrDNA base sequence of the female hormone-degrading microorganism and then detecting the amplified product obtained by the PCR method. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：待解决的问题：提供一种用于在短时间内具体评价具有雌性激素降解能力的雌激素降解微生物的量的方法。解决方案：用于检测存在于活性污泥等中的雌性激素降解微生物的方法包括使来自存在于活性污泥中的雌性激素降解微生物的核酸试剂等使用引物设计在雌激素降解微生物的16SrDNA碱基序列的共有区域，然后检测通过PCR方法获得的扩增产物。版权所有（C）2005，JPO＆NCIPI

9. 发明专利

JP2005125149A Method and apparatus for anaerobically treating organic waste 有权
标题翻译：用于厌氧处理有机废物的方法和装置
公开(公告)号：JP2005125149A
公开(公告)日：2005-05-19
申请号：JP2003360718
申请日：2003-10-21
申请人： Ebara Corp , 株式会社荏原製作所
发明人： KATAOKA NAOAKI , SHOBU SHOHEI , MIYA AKIKO
IPC分类号： B09B3/00 , C02F11/04
CPC分类号： Y02E50/343 , Y02W30/20
摘要： PROBLEM TO BE SOLVED: To provide a hydrogen fermentation/methane fermentation treatment method for treating solid-containing organic waste such as sewage sludge, waste sludge, livestock excreta, garbage and the waste of a food factory, and in particular to optimize a biological treatment process for simply and efficiently solubilizing/degrading the organic solid content into lower molecular weight materials in a short time by making good use of the reactive characteristics of an anaerobic microbe into a hydrogen/methane two-stage fermentation process. SOLUTION: The method and an apparatus for anaerobically treating organic waste are constituted so that hydrogen and methane are recovered biologically by a two-stage fermentation process consisting of an anaerobic solubilization/hydrogen fermentation stage as the former stage of two stages and a methane fermentation stage as the latter stage. The anaerobic treatment is a biological reaction carried out at 30 to 70°C at pH 5 to 8.5. The organic waste is fermented in two stages by returning a part of an outflow component from the methane fermentation stage to the solubilization/hydrogen fermentation stage. It is preferable that a hydraulic residence time of the solubilization/hydrogen fermentation stage is 0.2 to 5 days, that of the methane fermentation step is 0.2 to 2 days and that of a solubilization step is 3 to 4.8 days. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：待解决的问题：提供用于处理固体含有机废物如污水污泥，废污泥，家畜排泄物，垃圾和食品厂废物的氢发酵/甲烷发酵处理方法，特别是优化通过充分利用厌氧微生物的反应特性进入氢/甲烷两阶段发酵过程，在短时间内简单且有效地将有机固体成分溶解/降解成较低分子量材料的生物处理方法。解决方案：用于厌氧处理有机废物的方法和装置构成为通过由厌氧增溶/氢发酵阶段组成的两阶段发酵过程生物地回收氢和甲烷作为前两阶段的阶段和甲烷发酵阶段为后期。厌氧处理是在pH5〜8.5下在30〜70℃下进行的生物反应。通过将部分流出物从甲烷发酵阶段返回到溶解/氢发酵阶段，有机废物分两个阶段发酵。溶解/氢发酵阶段的液压停留时间优选为0.2〜5天，甲烷发酵工序的液压停留时间为0.2〜2天，增溶步骤为3〜4.8天。版权所有（C）2005，JPO＆NCIPI

10. 发明专利

JP2004113067A New microbial strain having cellulose degradation performance, using method therefor and growth promotion method 审中-公开
公开(公告)号：JP2004113067A
公开(公告)日：2004-04-15
申请号：JP2002279233
申请日：2002-09-25
申请人： Ebara Corp , 株式会社荏原製作所
发明人： TAMATSUBO KAZUAKI , NAGAYA YOSHIKI , MIYA AKIKO
IPC分类号： C12N1/20 , B09B3/00 , C02F3/28 , C02F3/34 , C02F11/04 , C12R1/145
CPC分类号： Y02E50/343 , Y02W30/20
摘要： PROBLEM TO BE SOLVED: To obtain a new microbial strain having cellulose degradation performance and to provide a using method therefor and a growth promotion method. SOLUTION: The new microbial strain is a strain which has cellulose degradation performance, comprises a base sequence of 16S rRNA gene exhibiting ≥90% homology to a sequence described by sequence number 1 and/or a base sequence of a cellulase gene exhibiting ≥80% homology to a sequence described by a sequence number 2 and belongs to genus Clostridium. Typically, Clostridium sp. JC3 strain (FERM P-19026) may be cited as the strain. The new microbial strain is used together with an acid fermentation and a methane fermentation bacteria in a cellulose-containing organic waste and/or waste water so that the cellulose contained in the organic waste and/or waste water is decomposed and recovered as methane gas. COPYRIGHT: (C)2004,JPO

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