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1. 发明专利

JP2005254168A Method and apparatus for anaerobic treatment of treatment object containing cellulosic organic material 有权
标题翻译：用于治疗含有细胞有机材料的治疗对象的方法和装置
公开(公告)号：JP2005254168A
公开(公告)日：2005-09-22
申请号：JP2004070793
申请日：2004-03-12
申请人： Ebara Corp , 株式会社荏原製作所
发明人： MIYA AKIKO , NAGAYA YOSHIKI , TAMATSUBO KAZUAKI
IPC分类号： C12N15/09 , B09B3/00 , C02F3/00 , C02F3/28 , C02F3/34 , C02F11/04 , C12M1/00 , C12N1/20
CPC分类号： Y02E50/343
摘要： PROBLEM TO BE SOLVED: To provide an aerobic treatment method and apparatus by which the cellulose degradation power in a treatment object containing a cellulosic organic material can be enhanced and maintained easily and inexpensively. SOLUTION: The anaerobic treatment method for the biodegradation of a treatment object containing a cellulosic organic material by cellulose degradative bacteria and includes the step of collecting at least a part of the treatment object to be subjected to an anaerobic treatment such as one based on methane fermentation, the step of giving, to the collected part, a cultivation environment wherein the number of the cellulose degradative bacteria increases, and the step of introducing at least a part of the cultivation-treated object into the treatment object. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供一种好氧处理方法和装置，通过该方法和装置，可以容易且廉价地提高和维持含有纤维素有机材料的处理对象中的纤维素降解能力。解决方案：用于通过纤维素降解细菌对含有纤维素有机材料的处理对象的生物降解的厌氧处理方法，包括收集至少一部分待进行厌氧处理的处理对象的步骤，例如基于对于甲烷发酵，向所收集的部分提供纤维素降解菌的数量增加的培养环境的步骤，以及将至少一部分培养处理对象引入处理对象的步骤。版权所有（C）2005，JPO＆NCIPI

2. 发明专利

JP2004261125A Method for evaluating cellulose decomposing ability of environmental specimen and oligonucleotide therefor 有权
公开(公告)号：JP2004261125A
公开(公告)日：2004-09-24
申请号：JP2003056088
申请日：2003-03-03
申请人： Ebara Corp , 株式会社荏原製作所
发明人： NAGAYA YOSHIKI , TAMATSUBO KAZUAKI , MIYA AKIKO
IPC分类号： G01N33/53 , B09B3/00 , C02F11/02 , C12N15/09 , C12Q1/68 , G01N33/569
摘要： PROBLEM TO BE SOLVED: To provide a method for evaluating the cellulose decomposing ability of an environmental specimen by determining the number of cells of cellulose decomposing bacteria in the environmental specimen. SOLUTION: The cellulose decomposing ability of an environmental specimen is evaluated by measuring the number of cells of cellulose decomposing bacteria having the following properties (1) to (4) in the specimen. (1) The bacterium belongs to the genus Clostridium; (2) the optimum growth temperature is within the temperature range of 45-65°C; (3) it has cellulose decomposing ability; and (4) it contains a 16SrRNA gene having at least 90% homology to a specific 16SrRNA gene base sequence derived from Clostridium genus and/or a cbhA gene having at least 80% homology to a specific cbhA gene base sequence derived from Clostridium genus. COPYRIGHT: (C)2004,JPO&NCIPI

3. 发明专利

JP2005253429A Method for detecting high temperature anaerobic protein-degrading bacterium and oligonucelotide for detection 审中-公开
标题翻译：检测高温厌氧蛋白降解细菌和寡核苷酸检测方法
公开(公告)号：JP2005253429A
公开(公告)日：2005-09-22
申请号：JP2004073169
申请日：2004-03-15
申请人： Ebara Corp , 株式会社荏原製作所
发明人： NAGAYA YOSHIKI , TATEZAWA TOMOKO , KARUBE MAKIKO , MIYA AKIKO , TAMATSUBO KAZUAKI
IPC分类号： C12N15/09 , B09B3/00 , C02F11/04 , C12N1/00 , C12Q1/68
CPC分类号： Y02E50/343
摘要： PROBLEM TO BE SOLVED: To provide a method for evaluating a protein-degrading ability in an environmental sample. SOLUTION: This method for evaluating a protein-degrading ability in an environmental sample, comprising measuring a protein-degrading bacterium by a quantitative PCR method using the following primer set. (a) An oligonucleotide having the first specific base sequence or its substantially homologous base sequence, and an oligonucleotide having the second specific base sequence or its substantially homologous base sequence. (b) An oligonucleotide having the third specific base sequence or its substantially homologous base sequence, and an oligonucleotide having the second specific base sequence or its substantially homologous base sequence. (c) An oligonucleotide having the fourth specific base sequence or its substantially homologous base sequence, and an oligonucleotide having the fifth specific base sequence or its substantially homologous base sequence. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：待解决的问题：提供一种评估环境样品中蛋白质降解能力的方法。解决方案：用于评估环境样品中蛋白质降解能力的方法，包括使用以下引物组通过定量PCR方法测量蛋白质降解细菌。（a）具有第一特异碱基序列或其基本上同源的碱基序列的寡核苷酸，以及具有第二特异碱基序列或其基本上同源的碱基序列的寡核苷酸。（b）具有第三特异碱基序列或其基本同源碱基序列的寡核苷酸，以及具有第二特异碱基序列或其基本上同源的碱基序列的寡核苷酸。（c）具有第四特异碱基序列或其基本上同源的碱基序列的寡核苷酸和具有第五特异碱基序列或其基本上同源的碱基序列的寡核苷酸。版权所有（C）2005，JPO＆NCIPI

4. 发明专利

JP2004113067A New microbial strain having cellulose degradation performance, using method therefor and growth promotion method 审中-公开
公开(公告)号：JP2004113067A
公开(公告)日：2004-04-15
申请号：JP2002279233
申请日：2002-09-25
申请人： Ebara Corp , 株式会社荏原製作所
发明人： TAMATSUBO KAZUAKI , NAGAYA YOSHIKI , MIYA AKIKO
IPC分类号： C12N1/20 , B09B3/00 , C02F3/28 , C02F3/34 , C02F11/04 , C12R1/145
CPC分类号： Y02E50/343 , Y02W30/20
摘要： PROBLEM TO BE SOLVED: To obtain a new microbial strain having cellulose degradation performance and to provide a using method therefor and a growth promotion method. SOLUTION: The new microbial strain is a strain which has cellulose degradation performance, comprises a base sequence of 16S rRNA gene exhibiting ≥90% homology to a sequence described by sequence number 1 and/or a base sequence of a cellulase gene exhibiting ≥80% homology to a sequence described by a sequence number 2 and belongs to genus Clostridium. Typically, Clostridium sp. JC3 strain (FERM P-19026) may be cited as the strain. The new microbial strain is used together with an acid fermentation and a methane fermentation bacteria in a cellulose-containing organic waste and/or waste water so that the cellulose contained in the organic waste and/or waste water is decomposed and recovered as methane gas. COPYRIGHT: (C)2004,JPO

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