专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明专利

JP2011229488A Method for real-time nucleic acid amplification by droplet manipulation 有权
标题翻译：用于实时核酸放大的方法
公开(公告)号：JP2011229488A
公开(公告)日：2011-11-17
申请号：JP2010104580
申请日：2010-04-28
申请人： Shimadzu Corp , 株式会社島津製作所
发明人： OHASHI TETSUO , WATANABE MASAYUKI
IPC分类号： C12Q1/68 , C12N15/09
CPC分类号： C12Q1/686 , C12Q2527/101 , C12Q2527/15 , C12Q2561/113 , C12Q2563/143 , C12Q2563/159
摘要： PROBLEM TO BE SOLVED: To provide a method of real-time amplification of nucleic acid, capable of speedily and accurately detecting fluorescence obtained in a nucleic acid amplification method performed in droplets in a complete closed system.SOLUTION: The method of real-time amplification of nucleic acid is a nucleic acid amplification reaction method for performing the nucleic acid amplification reaction in droplets present in a container. In the method of real-time amplification of nucleic acid, the droplets are made of nucleic acid amplification reactive solution containing nucleic acid to be amplified and magnetic particles, and the container holds a droplet-encapsulation medium which is not mixed with the nucleic acid amplification reactive solution forming the droplets and has a carrying surface being under a temperature gradient. A fluorescent dye is contained at least in the droplet-encapsulated medium among the droplets and droplet-encapsulation medium at the start of the nucleic acid amplification reaction. A magnetic field is generated by a magnetic field applying means at the start and retention of the nucleic acid amplification reaction, and the droplets are carried together with magnetic particles, and by disposing the droplets at a temperature point to achieve the start and retention of the nucleic acid synthetic reaction on the carrying surface, the temperature of the reactive solution is controlled.
摘要翻译：要解决的问题：提供一种能够快速准确地检测在完全封闭系统中以液滴的核酸扩增方法获得的荧光的核酸的实时扩增方法。解决方案：核酸的实时扩增方法是用于在存在于容器中的液滴中进行核酸扩增反应的核酸扩增反应方法。在核酸的实时扩增方法中，液滴由含有待扩增核酸的核酸扩增反应溶液和磁性粒子构成，容器中保持不与核酸扩增混合的液滴包封培养基反应溶液形成液滴并具有处于温度梯度下的载体表面。在核酸扩增反应开始时，至少在液滴和液滴包封介质中的液滴包封的介质中至少含有荧光染料。在开始和保留核酸扩增反应时，由磁场施加装置产生磁场，并且液滴与磁性颗粒携带在一起，并且通过将液滴设置在温度点以达到起始和保持在载体表面上进行核酸合成反应，控制反应溶液的温度。版权所有（C）2012，JPO＆INPIT

2. 发明专利

JP2009162580A Liquid drop manipulator and liquid drop manipulating method 审中-公开
标题翻译：液体液压操纵机构及液压操作方法
公开(公告)号：JP2009162580A
公开(公告)日：2009-07-23
申请号：JP2007341008
申请日：2007-12-28
申请人： Shimadzu Corp , 株式会社島津製作所
发明人： OHASHI TETSUO
IPC分类号： G01N35/08 , B01J19/00 , B01J19/08 , C12N15/09 , C12Q1/68 , G01N33/543 , G01N37/00
摘要： PROBLEM TO BE SOLVED: To provide a liquid drop manipulator of a simple structure having high design flexibility, and used for manipulating liquid drops with high accuracy without providing any physical fluid control mechanism, and a liquid drop manipulating method.
SOLUTION: This liquid drop manipulator is equipped with a liquid drop carrier having a carrying surface for thereon carrying liquid drops consisting of water solution containing magnetic particles, an electric field means for placing an electric field extending from below the drop carrier to above the carrying surface to fix the liquid drops on the carrying surface, a liquid drop carrier 10 having a carrying surface 10a for thereon carrying hydrophilic liquid drops 2 containing magnetic particles 2a, an electric field means 20 for placing an electric field stretching from below the drop carrier 10 to above the carrying surface 10a to fix the liquid drops 2 on the carrying surface 10a, and a magnetic field means 3 for placing a magnetic field extending from below the drop carrier 10 to above the carrying surface 10a to horizontally provide the magnetic particles 2a with force in a direction level with the carrying surface, the force being larger than the sum of fixation force of the liquid drops caused by the electric field placed thereon and surface tension of the liquid drops.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供一种具有高设计灵活性的简单结构的液滴操纵器，并且用于高精度地操作液滴而不提供任何物理流体控制机构，以及液滴操纵方法。解决方案：该液滴式操纵器配备有液滴载体，其具有用于承载由含有磁性颗粒的水溶液组成的液滴的载体表面的载液表面，用于将从液滴载体下方延伸的电场放置在上方的电场装置承载表面将液滴固定在承载表面上，液滴载体10具有承载表面10a，用于在其上承载含有磁性颗粒2a的亲水性液滴2，电场装置20，用于放置从下面延伸的电场载体10到承载表面10a上方以将液滴2固定在承载表面10a上;以及磁场装置3，用于将从液滴载体10的下方延伸的磁场放置在承载表面10a上方，以水平地提供磁性颗粒具有与承载表面一致的方向的力的力2a大于该固定力的总和由放置在其上的电场引起的液滴和液体的表面张力下降。版权所有（C）2009，JPO＆INPIT

3. 发明专利

JP2006250621A Sample injection method of capillary plate 有权
标题翻译：毛细管样品注射方法
公开(公告)号：JP2006250621A
公开(公告)日：2006-09-21
申请号：JP2005065527
申请日：2005-03-09
申请人： Shimadzu Corp , 株式会社島津製作所
发明人： OHASHI TETSUO , NAKAMURA SHIN , ENDO NAOYA
IPC分类号： G01N27/447 , G01N30/46 , G01N30/60 , G01N35/08 , G01N37/00
CPC分类号： C07K1/26
摘要： PROBLEM TO BE SOLVED: To inject a very small amount of a sample by suppressing the drying of the sample. SOLUTION: One end (cathode end) of each of separation flow channels 12 is connected to each of small capacity reservoirs 14a being sample reservoirs respectively opened to the surface of a substrate and a large capacity reservior 16a having a size enough to include all of the small capacity reservoirs 14a is surrounded by a wall 8 to be formed on the surface of the substrate. The other end (anode end) of each of the separation flow channels 12 is opened so as to be connected to the common reservior 16b formed to the surface of the substrate. A first liquid is put in the large capacity reservior so as to fill the small capacity reserviors 14a and the sample dissolved in a second liquid of which the specific gravity is higher than that of the first liquid is passed through the first liquid to be injected in the small capacity reserviors of respective capillary flow channels. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：通过抑制样品的干燥来注入非常少量的样品。解决方案：每个分离流动通道12的一端（阴极端）连接到每个小容量储存器14a，分别是开放到基板表面的样品储存器和大容量储存器16a，其尺寸足以包括所有的小容量储存器14a被形成在基板的表面上的壁8包围。每个分离流道12的另一端（阳极端）打开，以便连接到形成于基底表面的共同储存器16b。将第一液体放入大容量储存器中以填充小容量贮存器14a，并且将溶解在比重比第一液体高的第二液体的样品通过待注入的第一液体各毛细血管通道的小容量储存器。版权所有（C）2006，JPO＆NCIPI

4. 发明专利

JP2006266921A Solvent for preparing analytical sample 有权
标题翻译：用于制备分析样品的溶剂
公开(公告)号：JP2006266921A
公开(公告)日：2006-10-05
申请号：JP2005086638
申请日：2005-03-24
申请人： Shimadzu Corp , 株式会社島津製作所
发明人： OHASHI TETSUO , NAKAMURA SHIN
IPC分类号： G01N27/447 , G01N37/00
CPC分类号： G01N27/44747
摘要： PROBLEM TO BE SOLVED: To restrain a sample solution from being enriched to inject even a micro amount of a sample. SOLUTION: The analytical sample is prepared with a solvent for preparing the analytical sample constituted of water as a basic component, and containing at least one component selected from a group comprising a polyhydric alcohol, saccharides and a hydrophilic polymer. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：待解决的问题：为了抑制样品溶液富集以注入甚至微量的样品。解决方案：用溶剂制备分析样品，用于制备由水作为碱性组分构成的分析样品，并含有至少一种选自多元醇，糖类和亲水性聚合物的组分。版权所有（C）2007，JPO＆INPIT

5. 发明专利

JP2013130548A Chip device for manipulating object component, and method using the same 有权
标题翻译：用于操纵对象组件的芯片设备及使用该对象组件的方法
公开(公告)号：JP2013130548A
公开(公告)日：2013-07-04
申请号：JP2011282185
申请日：2011-12-22
申请人： Shimadzu Corp , 株式会社島津製作所
发明人： KATADOKORO ISAO , OHASHI TETSUO
IPC分类号： G01N35/08 , C12M1/00 , C12M1/34 , G01N37/00
CPC分类号： C12Q1/6806 , B01L3/502707 , B01L3/502761 , B01L7/52 , B01L2200/0668 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/165 , B01L2400/043 , G01N33/04 , G01N33/48707 , G01N35/0098 , H02K44/02 , Y10T29/49 , C12Q2565/629
摘要： PROBLEM TO BE SOLVED: To provide a device which allows a plurality of liquid substances such as reagents required for a series of manipulations of a sample to be stably fixed in a partitioned state, respectively, in the device through before and after the manipulations.SOLUTION: A chip device for manipulating an object component comprises: a substrate 2; a groove 3 formed on the substrate surface; a manipulation chip 1 including manipulation media composed of gel phases g and aqueous liquid phases l which are alternately arranged in the groove 3 in longitudinal direction and accommodated in contact with each other; magnetic material particles for capturing and transporting the object component; and magnetic field application means which allows the magnetic material particles to be moved in longitudinal direction of the groove on the substrate with application of a magnetic field to the substrate.
摘要翻译：要解决的问题：提供一种装置，其允许在操作之前和之后使装置中的多个液体物质例如样品的一系列操纵所需的试剂分别稳定地固定在装置中。：用于操纵物体部件的芯片装置包括：基板2; 形成在基板表面上的槽3; 操作芯片1，其包括由凝胶相g和水性液相1组成的操作介质，其在纵向方向上交替地布置在槽3中并且彼此接触; 磁性材料颗粒，用于捕获和运送物体成分; 以及磁场施加装置，其允许磁性材料颗粒在衬底上的凹槽的纵向方向上移动，同时向衬底施加磁场。

6. 发明专利

JP2011232260A Device using gel droplet encapsulation medium and method for operating droplet 有权
标题翻译：使用凝胶渗滤液的装置和操作喷雾器的方法
公开(公告)号：JP2011232260A
公开(公告)日：2011-11-17
申请号：JP2010104581
申请日：2010-04-28
申请人： Shimadzu Corp , 株式会社島津製作所
发明人： OHASHI TETSUO
IPC分类号： G01N35/08 , C12Q1/68 , G01N37/00
CPC分类号： C12Q1/6806 , B01L3/502761 , B01L3/502784 , B01L7/54 , B01L2200/0647 , B01L2200/0673 , B01L2300/069 , B01L2300/087 , B01L2300/12 , B01L2400/043 , Y10T137/0391 , Y10T137/206 , C12Q2531/113 , C12Q2525/131
摘要： PROBLEM TO BE SOLVED: To provide a droplet operation method capable of operating droplets only by means of operating a magnetic field without using a physical operation such as operating an electric field, and a droplet operation device which realizes the method.SOLUTION: The droplet operation device for transporting droplets in a droplet encapsulation medium comprises: a vessel 4 for holding the droplet encapsulation medium; droplets 12, 13, and 14 composed of an aqueous liquid; a gel droplet encapsulation medium 31 that is insoluble or poorly soluble in the aqueous liquid; magnetic particles 8 contained in droplets composed of the aqueous liquid; and magnetic field application means 61 for generating a magnetic field to thereby transport the droplets together with the magnetic particles. A method for operating droplets in a droplet encapsulation medium held in a vessel using the device is also provided.
摘要翻译：要解决的问题：提供一种仅通过在不使用诸如操作电场的物理操作的情况下操作磁场来操作液滴的液滴操作方法，以及实现该方法的液滴操作装置。解决方案：用于在液滴封装介质中输送液滴的液滴操作装置包括：用于保持液滴封装介质的容器4; 液滴12,13和14由水性液体组成; 在水性液体中不溶或难溶的凝胶微滴包封介质31; 包含在由含水液体组成的液滴中的磁性颗粒8; 以及用于产生磁场从而将液滴与磁性颗粒一起输送的磁场施加装置61。还提供了使用该装置在保持在容器中的液滴封装介质中操作液滴的方法。版权所有（C）2012，JPO＆INPIT

7. 发明专利

JP2014221061A 管内で対象成分を操作するための操作管有权
标题翻译：用于操作管道中的目标组件的操作管
公开(公告)号：JP2014221061A
公开(公告)日：2014-11-27
申请号：JP2014136442
申请日：2014-07-02
申请人：株式会社島津製作所 , Shimadzu Corp
发明人： OHASHI TETSUO
IPC分类号： C12M1/00
CPC分类号： G01N35/0098 , B01J2219/00364 , B01J2219/00466 , B01J2219/00468 , B01L3/5025 , B01L3/5082 , B01L2200/0647 , B01L2200/0673 , B01L2300/0832 , B01L2300/0861 , B01L2300/087 , B01L2400/043 , B03C1/00 , B03C1/12 , B03C1/288 , Y10T29/49826
摘要：【課題】磁性粒子を用いて汚染源の発生を極力抑えつつ、所望の一連の操作の全てを行うことができる、小型且つ低ランニングコストのデバイスの提供。【解決手段】操作管1内で対象成分を操作するためのデバイスであって:一方に対象成分を含む試料を供給するための閉鎖可能であってよい開口端5及び他方に閉口端を有する管と、前記管内に収容され且つゲル層g,3g及び水系液体層3l,lが管の長手方向に重層した操作用媒体3とを含む操作管1と;対象成分を運搬させるべき磁性体粒子6と;操作管1に磁場を印加することによって磁性体粒子6を管の長手方向に移動させることができる磁場印加手段31とを含むデバイス。【選択図】図1
摘要翻译：要解决的问题：提供一种低运行成本的小型装置，这使得可以执行所有一系列操作，同时最大限度地通过使用磁性颗粒来抑制任何污染源的发生。解决方案：用于操作目标的装置操作管1中的部件包括：操作管1，其包括在一侧上具有开口端5的管，该管可以关闭以便供应包含目标部件并且在另一侧具有封闭端的样本，操作介质3容纳在管中并具有在管的纵向方向上层叠的凝胶层g，3g和水性液体层31l，磁性材料颗粒6，目标组分将与其一起运输; 以及磁场施加装置31，其通过向操作管1施加磁场使得能够沿着管的纵向方向移动磁性材料颗粒6。

8. 发明专利

JP2008089440A Electrophoresis apparatus 有权
标题翻译：电泳装置
公开(公告)号：JP2008089440A
公开(公告)日：2008-04-17
申请号：JP2006271397
申请日：2006-10-03
申请人： Shimadzu Corp , 株式会社島津製作所
发明人： NAKAMURA SHIN , YAMAMOTO RINTARO , HARADA TORU , HAZAMA HAJIME , IKEGAMI TAKASHI , KACHI TORU , INAMI JIYUNJI , OHASHI TETSUO , UTSUNOMIYA SHINICHI , MIYAMOTO KEISUKE , ENDO NAOYA , KUMAGAI HIDESATO
IPC分类号： G01N27/447 , G01N35/10
CPC分类号： G01N27/44791 , G01N27/44721 , G01N27/44743 , G01N27/44782
摘要： PROBLEM TO BE SOLVED: To automate the operation of an electrophoresis apparatus and to improve the throughput. SOLUTION: This electrophoresis apparatus has two platens 2a and 2b capable of controlling temperature of electrophoresis plates placed thereon, a loading medium charging unit 8 for sending a loading medium under pressure, a loading medium charging nozzle mechanism 10 having a pair of nozzles 12a and 12b connected to the loading medium charging unit 8, a pipetter mechanism for dispensing samples to sample dispensing openings of the electrophoresis plates placed on the platens 2a and 2b, a stacker mechanism 16 for storing sample plates, a loading buffer solution supplying mechanism 20, a loading buffer solution dispensing mechanism having an injection nozzle connected to the loading buffer solution supplying mechanism, a power unit for allowing electrophoresis separation for each electrophoresis plate placed on the platens 2a and 2b, and a detector for optically detecting components migrating through each electrophoresis flow channel of the electrophoresis plates. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：要解决的问题：使电泳装置的操作自动化并提高生产量。解决方案：该电泳装置具有能够控制放置在其上的电泳板的温度的两个压板2a和2b，用于在压力下发送装载介质的装载介质充电单元8，具有一对喷嘴的装载介质装料喷嘴机构10 12a和12b连接到装载介质充电单元8，用于分配样品以移送到放置在压板2a和2b上的电泳板的样品分配开口的移液器机构，用于存储样品板的堆积器机构16，装载缓冲溶液供应机构20 ，具有连接到装载缓冲溶液供给机构的注射喷嘴的装载缓冲溶液分配机构，用于对放置在台板2a和2b上的每个电泳板进行电泳分离的动力单元和用于光学检测通过每个电泳迁移的组分的检测器电泳板的流路。版权所有（C）2008，JPO＆INPIT

9. 发明专利

JP2008012490A Method and apparatus for performing microchemical reaction 有权
标题翻译：用于微化学反应的方法和装置
公开(公告)号：JP2008012490A
公开(公告)日：2008-01-24
申请号：JP2006188708
申请日：2006-07-07
申请人： Shimadzu Corp , 株式会社島津製作所
发明人： OHASHI TETSUO
IPC分类号： B01J19/08 , B01J19/00 , C12M1/00 , C12N15/09 , G01N37/00
摘要： PROBLEM TO BE SOLVED: To provide a method and an apparatus for performing a chemical reaction, in each of which operations necessary for the chemical reaction such as the sending, collection, separation, mixing, dilution, agitation and temperature control of a liquid can be performed without using fluid control elements such as a pump, a valve and a mixer.
SOLUTION: The method for performing the chemical reaction, in which the chemical reaction is performed in the liquid of a liquid droplet existing in a reaction vessel or on the surface of a reaction substrate, comprises the steps of: imparting fluctuations of a magnetic filed to the liquid droplet formed from an aqueous solution containing particles of a magnetic material having a hydrophilic surface, so that the magnetic material transmits physical force to the surrounding aqueous solution and moves the liquid droplet to perform operations necessary for the chemical reaction; and arranging a temperature changeable area, in which the temperature changes continuously, in the reaction vessel or on the reaction substrate, placing the liquid droplet at at least one spot in the temperature changeable area by using fluctuations of the magnetic field to control the temperature of the liquid droplet and performing the chemical reaction.
COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：要解决的问题：为了提供用于进行化学反应的方法和装置，在其中进行化学反应所需的操作，例如发送，收集，分离，混合，稀释，搅拌和温度控制可以在不使用诸如泵，阀和混合器的流体控制元件的情况下执行液体。解决方案：用于进行化学反应的方法，其中在反应容器中或反应基材表面上的液滴的液体中进行化学反应，包括以下步骤：对由含有具有亲水表面的磁性材料的颗粒的水溶液形成的液滴进行磁场磁化，使得磁性材料向周围的水溶液传递物理力并移动液滴，进行化学反应所需的操作; 并且在反应容器或反应基板上排列温度连续变化的温度变化区域，通过使用磁场的波动将液滴放置在温度变化区域的至少一个点上，以控制温度的变化液滴并进行化学反应。版权所有（C）2008，JPO＆INPIT

10. 发明专利

JPH05123197A METHOD FOR DETECTING NUCLEIC ACID 失效
公开(公告)号：JPH05123197A
公开(公告)日：1993-05-21
申请号：JP28475591
申请日：1991-10-30
申请人： SHIMADZU CORP
发明人： TADA ATSUSHI , OHASHI TETSUO
IPC分类号： C12Q1/68 , C12N15/09 , G01N33/50 , G01N33/52
摘要： PURPOSE:To carry out detection of the objective nucleic acid in high sensitivity and short time without carrying out electrophoresis. CONSTITUTION:A specific DNA is proliferated by a PCR method using two kinds of oligonucleotides in which at least one is labeled as a chain extending reaction primer and then the resultant reaction product is added to a microplate fixing a label-specific substance used in the above-mentioned reaction to the labeled oligonucleotide containing a part of a proliferation fragment and complimentary sequence and signal produced by the label is measured to detect whether or not the objective nucleic acid exists.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式