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1. 发明专利

JP2008089440A Electrophoresis apparatus 有权
标题翻译：电泳装置
公开(公告)号：JP2008089440A
公开(公告)日：2008-04-17
申请号：JP2006271397
申请日：2006-10-03
申请人： Shimadzu Corp , 株式会社島津製作所
发明人： NAKAMURA SHIN , YAMAMOTO RINTARO , HARADA TORU , HAZAMA HAJIME , IKEGAMI TAKASHI , KACHI TORU , INAMI JIYUNJI , OHASHI TETSUO , UTSUNOMIYA SHINICHI , MIYAMOTO KEISUKE , ENDO NAOYA , KUMAGAI HIDESATO
IPC分类号： G01N27/447 , G01N35/10
CPC分类号： G01N27/44791 , G01N27/44721 , G01N27/44743 , G01N27/44782
摘要： PROBLEM TO BE SOLVED: To automate the operation of an electrophoresis apparatus and to improve the throughput. SOLUTION: This electrophoresis apparatus has two platens 2a and 2b capable of controlling temperature of electrophoresis plates placed thereon, a loading medium charging unit 8 for sending a loading medium under pressure, a loading medium charging nozzle mechanism 10 having a pair of nozzles 12a and 12b connected to the loading medium charging unit 8, a pipetter mechanism for dispensing samples to sample dispensing openings of the electrophoresis plates placed on the platens 2a and 2b, a stacker mechanism 16 for storing sample plates, a loading buffer solution supplying mechanism 20, a loading buffer solution dispensing mechanism having an injection nozzle connected to the loading buffer solution supplying mechanism, a power unit for allowing electrophoresis separation for each electrophoresis plate placed on the platens 2a and 2b, and a detector for optically detecting components migrating through each electrophoresis flow channel of the electrophoresis plates. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：要解决的问题：使电泳装置的操作自动化并提高生产量。解决方案：该电泳装置具有能够控制放置在其上的电泳板的温度的两个压板2a和2b，用于在压力下发送装载介质的装载介质充电单元8，具有一对喷嘴的装载介质装料喷嘴机构10 12a和12b连接到装载介质充电单元8，用于分配样品以移送到放置在压板2a和2b上的电泳板的样品分配开口的移液器机构，用于存储样品板的堆积器机构16，装载缓冲溶液供应机构20 ，具有连接到装载缓冲溶液供给机构的注射喷嘴的装载缓冲溶液分配机构，用于对放置在台板2a和2b上的每个电泳板进行电泳分离的动力单元和用于光学检测通过每个电泳迁移的组分的检测器电泳板的流路。版权所有（C）2008，JPO＆INPIT

2. 发明专利

JP2003098154A SAMPLE PREPARATOR FOR MASS SPECTROMETRY 审中-公开
公开(公告)号：JP2003098154A
公开(公告)日：2003-04-03
申请号：JP2001288609
申请日：2001-09-21
申请人： SHIMADZU CORP , JAPAN SCIENCE & TECH CORP
发明人： YAMAGUCHI AKIRA , IKEGAMI TAKASHI , SUGIYAMA KIYOHIRO , AKINAGA NOBUYUKI
IPC分类号： G01N27/64 , G01N1/00 , G01N1/28 , G01N27/62 , H01J49/04
摘要： PROBLEM TO BE SOLVED: To easily specify a location of laser irradiation for ionization. SOLUTION: Air is supplied by bringing a micro nozzle 12 for supplying air closely to one end of sample solution 8. In a dispensation spot 6, evaporation from the surface of the sample solution 8 is activated at a part supplied with air and crystallization progresses abruptly as compared with other parts. Subsequently, a gradient of concentration is generated in the sample solution due to local crystallization thereof and noncrystallized solute flows to the vicinity of a crystal and crystallizes. As a result, a crystal grows around the part supplied with air. Optimal location of laser irradiation can be facilitated for such a crystal and high speed automatic processing can be realized by delivering the positional information of the micro nozzle for air supply to a TOFMS and determining the laser irradiation position based on that positional information.

3. 发明专利

JP2003098149A SAMPLE PREPARATOR FOR MASS SPECTROMETRY 审中-公开
公开(公告)号：JP2003098149A
公开(公告)日：2003-04-03
申请号：JP2001288599
申请日：2001-09-21
申请人： SHIMADZU CORP , JAPAN SCIENCE & TECH CORP
发明人： IKEGAMI TAKASHI , SUGIYAMA KIYOHIRO , YAMAGUCHI AKIRA , AKINAGA NOBUYUKI
IPC分类号： G01N27/62 , G01N1/28 , G01N27/64
摘要： PROBLEM TO BE SOLVED: To easily specify a location of laser irradiation for ionization. SOLUTION: A pin 42 is provided on the side face on one end side of a supporting table 40 secured with a sample plate 2, a supporting part 44 is provided on a base 21, and the pin 42 is fitted in the hole 46 at the supporting part 44. A rack 48 is provided on the end face of the supporting table 40 on the other end side thereof and meshed with a pinion 50. The rack 48 is pushed up when the pinion 50 turns and the fulcrum of the pin 42, i.e., the supporting base 40, inclines to cause inclination of the sample plate 2. When the sample plate 2 is inclined before or after dispensation of sample solution 8 and the sample solution 8 is dried under that state, crystallization takes place from the inclining lower part and a large quantity of sample is crystallized at the lower position. When a position corresponding to the low position at the time of crystallization is set as the laser irradiating position at the time of mass spectrometry, analysis can be carried out with high sensitivity.

4. 发明专利

JPH05329400A SPIN FILTER 失效
公开(公告)号：JPH05329400A
公开(公告)日：1993-12-14
申请号：JP13856992
申请日：1992-05-29
申请人： SHIMADZU CORP
发明人： IKEGAMI TAKASHI
IPC分类号： B03C5/00 , C12M1/12
摘要： PURPOSE:To enhance a filtration efficiency by preventing a main filter system from becoming clogged. CONSTITUTION:This spin filter 4 is used for separating cells and draining a culture liquid from a culture tank. The filter 4 is equipped with a rotatable main filter system 5 having a porous electroconductive cylinder and a voltage supply member 13 connected to the main filter system 5 for supplying a voltage of the same side as an electrically charged cell to the main filter system 5.

5. 发明专利

JPH0310676A BAG FOR CELL CULTURE 失效
公开(公告)号：JPH0310676A
公开(公告)日：1991-01-18
申请号：JP14711789
申请日：1989-06-09
申请人： SHIMADZU CORP
发明人： HAMAZAKI YUJI , IKEGAMI TAKASHI , YAMAMOTO RINTARO
IPC分类号： C12M3/06
摘要： PURPOSE:To obtain the subject bag capable of efficiently stirring a culture solution because of different-way movement of a stirring piece from that of the bag for cell culture by accommodating the stirring piece having a different specific gravity from that of the culture solution in the bag body for accommodating the cells and the culture solution. CONSTITUTION:A stirring piece 28 movable in a bag body 17 for cell culture and having a different specific gravity from that of a culture solution is accommodated in the bag body so that the culture solution may be stirred by the stirring piece 28 capable of different movement from that of the bag for cell culture when the cell culture is carried out while oscillating the bag for cell culture after putting cells and the culture solution into the bag body 17.

6. 发明专利

JP2003099439A RETRIEVING DEVICE FOR ANALYZING PROCESSING METHOD 审中-公开
公开(公告)号：JP2003099439A
公开(公告)日：2003-04-04
申请号：JP2001288840
申请日：2001-09-21
申请人： SHIMADZU CORP , JAPAN SCIENCE & TECH CORP
发明人： SUGIYAMA KIYOHIRO , IKEGAMI TAKASHI , YAMAGUCHI AKIRA , AKINAGA NOBUYUKI
IPC分类号： G06F17/30
摘要： PROBLEM TO BE SOLVED: To provide a retrieving method which is easy for a user to use. SOLUTION: In the picture of a sequence retrieving condition inputting window, when the attribute of a sequence is inputted by numerical values and characters, the sequence attribute memory of a database is opened and loaded on the memory of a personal computer main body. The coincidence of the loaded sequence attribute and the inputted attribute is checked, and points are calculated by each sequence. As the calculation method of these points, '1' is given as a score to a coinciding attribute and '0' is given to a non- coinciding attribute. Then, the product of the numerical value of weighting inputted by each attribute and the score of the attribute is taken and sums are taken extending all the attributes, thereby the points of coincidence concerning, the coincidence of the sequence is obtained. After this, the displaying order of the sequences is changed and displayed in order of the points.

7. 发明专利

JPH0310679A CELL CULTURE BAG 失效
公开(公告)号：JPH0310679A
公开(公告)日：1991-01-18
申请号：JP14712189
申请日：1989-06-09
申请人： SHIMADZU CORP
发明人： HAMAZAKI YUJI , IKEGAMI TAKASHI , TOZAKI NAMI , TAKANO JUN , YAMAGATA KOICHI
IPC分类号： C12M3/06
摘要： PURPOSE:To provide the subject bag capable of simplifying a process for obtaining an objective substance from a cultured solution and not reducing the activity of the objective substance by receiving in a bag main body an adsorbent specifically adsorbing the useful substance excreted from cultured cells. CONSTITUTION:An adsorbent 90 specifically adsorbing an useful substance excreted from cultured cells 91 is received in a bag main body 1 to provide the objective bag in which the cells and a culture medium are charged to culture the cells. The adsorbent 90 is usually composed from ligand portions relating to an interaction for the adsorption and matrix portions not relating to the interaction. The ligand is e.g. hydroxy apatite when the useful substance is cytokinin, and the matrix is e.g. alumina.

8. 发明专利

JPH0310677A CULTURE BAG 失效
公开(公告)号：JPH0310677A
公开(公告)日：1991-01-18
申请号：JP14711989
申请日：1989-06-09
申请人： SHIMADZU CORP
发明人： HAMAZAKI YUJI , IKEGAMI TAKASHI , TOZAKI MINA , TAKANO JUN , YAMAGATA KOICHI
IPC分类号： C12M1/34 , C12M3/06
摘要： PURPOSE:To provide the subject bag enabling to readily measure the density of cells therein and continuously monitor the density and not having the danger of the contamination of various bacteria by disposing an optical sensor in a bag main body receiving the cells and a culture medium to optically detect the density of the cells. CONSTITUTION:An optical sensor 25 is disposed in a bag main body 17 for receiving cells and a culture medium to provide the objective bag enabling to optically detect the density of the cells in the bag main body 17. The detection of the cell density is performed e.g. by detecting the degree of the scattering of light changed in response to the change of the cell density with the optical sensor and subsequently computing the detected cell density with a control section based on the output change of the optical sensor.

9. 发明专利

JPH1019847A ELECTROPHORETTC APPARATUS 失效
公开(公告)号：JPH1019847A
公开(公告)日：1998-01-23
申请号：JP18880896
申请日：1996-06-28
申请人： SHIMADZU CORP
发明人： IKEGAMI TAKASHI
IPC分类号： G01N27/447 , G01N27/26
摘要： PROBLEM TO BE SOLVED: To obtain an electrophoretic apparatus in which a liquid leak can be detected with good accuracy. SOLUTION: A voltage and current minitoring circuit 18 monitors the value of a voltage applied to an upper-part buffer tank 6 from a high-voltage generation part 16 when the voltage is input from the high-voltage generation part 16, and it detects a current flowing to a lower-part buffer tank 8. A CPU 12 finds the value of electric power on the basis of the value of the monitored voltage and on the basis of the value of the current as their product, a linear regression curve is found on the basis of a time-dependent change in the value of the electric power, and whether liquid peak exists or not is judged.

10. 发明专利

JPH037575A APPARATUS FOR CELL CULTURE 失效
公开(公告)号：JPH037575A
公开(公告)日：1991-01-14
申请号：JP14711889
申请日：1989-06-09
申请人： SHIMADZU CORP
发明人： HAMAZAKI YUJI , YAMAMOTO RINTARO , IKEGAMI TAKASHI , TOZAKI NAMI , TAKANO JUN , YAMAGATA KOICHI
IPC分类号： C12M3/00
摘要： PURPOSE:To omit labor in exchanging a culture solution and prevent a fear of contamination in the exchanging by rocking a bag pedestal having a culture bag fixed thereto about a vertical shaft and simultaneously replacing the culture solution synchronously with the rocking. CONSTITUTION:A culture bag 17 containing a diaphragm bag 19 housing T cell, IL2, etc., of patients suffering from cancer is placed on a tilted culture bag pedestal 52 and the corners of the bag 17 are fixed with fixing members 53. A motor 43 is then revolved to rotate a rotating shaft 41 through bevel gears 44 and 42 and the pedestal 52 is rocked on an intersecting point of the center line(O1) of a supporting part 50 tilted to the shaft 41 and the center line (O2) of the shaft 41 as the center. A lever 57 held by guide plates 58 is simultaneously driven to rock the pedestal 52 about the vertical shaft. On the other hand, a cam 45 is rotated simultaneously with the rotation of the shaft 41 to turn ON or OFF a microswitch 48. A squeeze pump is driven to introduce a fresh culture medium through a pipe 23 into the bag 17 and the old culture medium is simultaneously discharged through a culture medium discharge pipe 22.

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