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1. 发明公开

EP3201336A1 METHODS FOR EXTRACTING AND PURIFYING NON-DENATURED PROTEINS 有权转让
标题翻译：提取和纯化未降解蛋白质的方法
公开(公告)号：EP3201336A1
公开(公告)日：2017-08-09
申请号：EP15846579.9
申请日：2015-10-01
申请人： Impossible Foods Inc.
发明人： KALE, Aniket , VARADAN, Ranjani , DAVIS, Simon Christopher
IPC分类号： C12N9/88 , A61K36/81
CPC分类号： C12N9/88 , A23J1/006 , A61K36/21 , A61K2236/00 , B01D11/02 , B01D61/145 , C12Y401/01039
摘要： Materials and methods for extracting and purifying proteins are provided. For example, the materials and methods provided herein can be used for extracting and purifying proteins that denature at low temperature.
摘要翻译：提供了提取和纯化蛋白质的材料和方法。例如，本文提供的材料和方法可用于提取和纯化在低温下变性的蛋白质。

2. 发明公开

EP2631241A3 Process for generation of protein and uses thereof 失效
标题翻译：一种用于生产蛋白质的过程和其用途
公开(公告)号：EP2631241A3
公开(公告)日：2014-04-09
申请号：EP13168432.6
申请日：2007-10-10
申请人： The Australian National University
发明人： Gready, Jill , Kannappan, Babu
IPC分类号： C07K1/00 , C07K14/415 , C12N15/64 , C07H21/04 , C12N15/29 , C12N9/88
CPC分类号： C12N9/88 , C07K1/00 , C07K1/13 , C07K1/16 , C07K1/18 , C07K1/36 , C07K14/415 , C07K2299/00 , C12N15/1089 , C12Y401/01039
摘要： A method of generating a protein with an improved functional property, the method comprising: (a) identifying at least one Target amino acid Residue in a first protein, wherein said Target amino acid Residue is associated with said functional property; (b) comparing at least one homologous second protein from the same or a different phylogenetic branch as the first protein with the first protein and identifying at least one Variant amino acid Residue between the first protein and the second protein; (c) selecting at least one Candidate amino acid Residue from the Variant amino acid Residue identified in (b) on the basis of said Candidate amino acid Residue affecting said Target amino acid Residue with respect to said functional property; (d) forming at least one Candidate Mutant protein in silico or producing at least one Candidate Mutant protein in vitro in which said at least one Candidate amino acid Residue from the second protein substitutes a corresponding residue in the first protein; and (e) screening said at least one Candidate Mutant protein produced in (d) to identify a protein having said improved functional property.

3. 发明公开

EP2325197A2 Expression control elements from the Lemnaceae family 审中-公开
标题翻译：来自Lemnaceae家族的表达控制元件
公开(公告)号：EP2325197A2
公开(公告)日：2011-05-25
申请号：EP11154225.4
申请日：2007-01-17
申请人： Biolex Therapeutics, Inc.
发明人： Dickey, Lynn F. , Cox, Kevin M. , Peele, Charles G.
IPC分类号： C07K14/415 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8216 , C07K14/415 , C12N9/2442 , C12N9/88 , C12N15/8221 , C12N15/8222 , C12N15/8223 , C12Y302/01014 , C12Y401/01039
摘要： Compositions and methods for regulating expression of nucleotide sequences of interest in a plant are provided. Compositions include novel nucleic acid molecules, and variants and fragments thereof, for expression control elements isolated from the Lemnaceae ubiquitin, r-histone and chitinase genes. A method for expressing a nucleotide sequence of interest in a plant using the expression control elements disclosed herein is further provided. The method includes introducing into a plant or plant cell or nodule an expression construct comprising an expression control element of the present invention operably linked to a nucleotide sequence of interest. In particular, the compositions and methods find use in enhancing expression of nucleotide sequences of interest in duckweed. Also provided is a novel Lemnaceae signal peptide-encoding sequence and the signal peptide encoded thereby. Where an expression construct of the invention is designed to express a polypeptide of interest, this novel signal peptide-encoding sequence can be included within the expression construct of the invention to provide for extracellular secretion of the encoded polypeptide of interest.
摘要翻译：提供了用于调节植物中目的核苷酸序列表达的组合物和方法。组合物包括新颖核酸分子及其变体和片段，用于从宇木科泛素，r-组蛋白和几丁质酶基因分离的表达控制元件。进一步提供了使用本文公开的表达控制元件在植物中表达感兴趣的核苷酸序列的方法。该方法包括向植物或植物细胞或结节中导入包含与感兴趣的核苷酸序列可操作连接的本发明的表达控制元件的表达构建体。具体而言，所述组合物和方法可用于增强浮萍中感兴趣的核苷酸序列的表达。还提供了一种新颖的Lemnaceae信号肽编码序列和由此编码的信号肽。当设计本发明的表达构建体以表达感兴趣的多肽时，可将此新信号肽编码序列包括在本发明的表达构建体内以提供所编码的目的多肽的细胞外分泌。

4. 发明公开

EP4388113A1 METHODS FOR MODIFYING PLASTID GENOMES 审中-公开
公开(公告)号：EP4388113A1
公开(公告)日：2024-06-26
申请号：EP22859321.6
申请日：2022-08-16
申请人： Monsanto Technology LLC
发明人： YE, Xudong
IPC分类号： C12N15/82 , C12N9/22
CPC分类号： C12N9/22 , C12N15/8214 , C12N9/88 , C12Y401/01039

5. 发明授权

EP2079755B1 PROCESS FOR GENERATION OF PROTEIN AND USES THEREOF 失效
标题翻译：产生蛋白质的方法及其用途
公开(公告)号：EP2079755B1
公开(公告)日：2017-08-30
申请号：EP07815347.5
申请日：2007-10-10
申请人： The Australian National University
发明人： GREADY, Jill, E. , KANNAPPAN, Babu
IPC分类号： C07K1/00 , C07K14/415 , C12N15/64 , C07H21/04 , C12N15/29
CPC分类号： C12N9/88 , C07K1/00 , C07K1/13 , C07K1/16 , C07K1/18 , C07K1/36 , C07K14/415 , C07K2299/00 , C12N15/1089 , C12Y401/01039
摘要： A method of generating a protein with an improved functional property, the method comprising: (a) identifying at least one Target amino acid Residue in a first protein, wherein said Target amino acid Residue is associated with said functional property; (b) comparing at least one homologous second protein from the same or a different phylogenetic branch as the first protein with the first protein and identifying at least one Variant amino acid Residue between the first protein and the second protein; (c) selecting at least one Candidate amino acid Residue from the Variant amino acid Residue identified in (b) on the basis of said Candidate amino acid Residue affecting said Target amino acid Residue with respect to said functional property; (d) forming at least one Candidate Mutant protein in silico or producing at least one Candidate Mutant protein in vitro in which said at least one Candidate amino acid Residue from the second protein substitutes a corresponding residue in the first protein; and (e) screening said at least one Candidate Mutant protein produced in (d) to identify a protein having said improved functional property.
摘要翻译：产生具有改善的功能性质的蛋白质的方法，所述方法包括：（a）鉴定第一蛋白质中的至少一个靶氨基酸残基，其中所述靶氨基酸残基与所述功能性质相关; （b）将来自与第一蛋白质相同或不同的系统发生分支的至少一种同源第二蛋白质与第一蛋白质进行比较，并鉴定第一蛋白质与第二蛋白质之间的至少一种变体氨基酸残基; （c）基于影响所述靶氨基酸残基的所述候选氨基酸残基，根据所述功能性选择来自（b）中鉴定的变体氨基酸残基的至少一种候选氨基酸残基; （d）在计算机上形成至少一种候选突变蛋白或在体外产生至少一种候选突变蛋白，其中所述至少一种来自第二蛋白的候选氨基酸残基取代第一蛋白中的相应残基; 和（e）筛选（d）中产生的所述至少一种候选突变体蛋白质以鉴定具有所述改进的功能性质的蛋白质。

6. 发明公开

EP3094717A1 LEVURES MODIFIÉES POUR UTILISER LE DIOXYDE DE CARBONE 审中-公开
标题翻译：改良的ZUR VERWENDUNG KOHLENDIOXID
公开(公告)号：EP3094717A1
公开(公告)日：2016-11-23
申请号：EP15707418.8
申请日：2015-01-16
申请人： Institut National De La Recherche Agronomique , Institut National des Sciences Appliquées de Toulouse , Centre National de la Recherche Scientifique
发明人： POMPON, Denis , PÂQUES, Frédéric , LESAGE, Julie , GUILLOUET, Stèphane , BONNOT, Florence , MARC, Jillian , GORRET, Nathalie , BIDEAUX, Carine , BOUTONNET, Christel
IPC分类号： C12N1/16 , C12N9/88 , C12N9/12 , C12P7/06 , C12N15/81
CPC分类号： C12N15/81 , C07K14/195 , C12N9/1205 , C12N9/88 , C12N2830/002 , C12Y207/01019 , C12Y401/01039 , Y02E50/17
摘要： The invention relates to yeast cells modified to express a functional type I RuBisCO enzyme, and a class II phosphoribulokinase. The expression of these enzymes recreates a Calvin cycle in said yeasts in order to enable the yeasts to use carbon dioxide.
摘要翻译：本发明涉及修饰以表达功能性I型RuBisCO酶的酵母细胞和第二类磷酸核糖激酶。这些酶的表达在所述酵母中重新产生卡尔文循环，以使酵母能够使用二氧化碳。

7. 发明公开

EP2160399A4 NOVEL METHODS FOR RECOVERY OF LEAF PROTEINS 审中-公开
标题翻译：新方法回收叶片蛋白质
公开(公告)号：EP2160399A4
公开(公告)日：2010-09-15
申请号：EP08754483
申请日：2008-05-15
申请人： UNIV MARYLAND
发明人： LO MARTIN , FU HONG
IPC分类号： C07K1/14 , A61K36/81 , C07K1/30 , C07K14/415
CPC分类号： C12N9/88 , A61K36/81 , B01D9/0059 , B01D11/0288 , C07K1/145 , C07K1/30 , C07K1/36 , C12Y401/01039
摘要： A novel method for processing soluble plant leaf proteins is described. While leaf proteins are considered potentially the most abundant source of protein in nature, the lack of efficient processing techniques for leaf proteins has limited their commercial use. The method described in this patent provides a means of extracting and purifying leaf proteins from plants which is suitable for leaf protein production on an industrial scale.

8. 发明公开

EP4353087A1 MEAT ANALOGUE PRODUCTS 审中-公开
公开(公告)号：EP4353087A1
公开(公告)日：2024-04-17
申请号：EP22203394.6
申请日：2022-10-24
申请人： BK Giulini GmbH , Plantible Foods, Inc.
发明人： GRAHN, Irene , GERDES, Annika , SRICHUWONG, Sathaporn , SPORKA, Radovan , CILLIERS, Nina , DAVIS, Huntington
IPC分类号： A23L29/256 , A23J3/14 , A23J3/22 , A23L29/281
CPC分类号： A23J3/14 , A23J3/227 , A23L29/256 , A23L29/281 , C12Y401/01039 , C12N9/88
摘要： The composition of the present invention is suitable for use in preparing emulsion-type meat analogue, preferably as a sausage type in a casing, which comprises rubisco protein and kappa-carrageenan in relative amounts between 1:5 to 30:1. Generally, the composition comprises 0.5-30 wt% rubisco and 0.1-15 wt% carrageenan relative the finished meat analogue product, and vegan protein other than rubisco preferably in an amount of 0.5 - 30 wt% relative to the meat analogue product. The meat analogue may contain the composition described in an amount of between 2-40 wt% with 30-90 wt% water and 2-40 wt% plant-based lipid.

9. 发明公开

EP3201336A4 METHODS FOR EXTRACTING AND PURIFYING NON-DENATURED PROTEINS 有权转让
公开(公告)号：EP3201336A4
公开(公告)日：2018-03-07
申请号：EP15846579
申请日：2015-10-01
申请人： IMPOSSIBLE FOODS INC
发明人： KALE ANIKET , VARADAN RANJANI , DAVIS SIMON CHRISTOPHER
IPC分类号： C12N9/88 , A61K36/81
CPC分类号： C12N9/88 , A23J1/006 , A61K36/21 , A61K2236/00 , B01D11/02 , B01D61/145 , C12Y401/01039
摘要： Materials and methods for extracting and purifying proteins are provided. For example, the materials and methods provided herein can be used for extracting and purifying proteins that denature at low temperature.

10. 发明公开

EP2465515A1 Cyanobacterium of the family pseudanabaenaceae (oscillatoriales, cyanophyta), genus protolyngbya, species protolyngbya sp. Strain ITD-01 审中-公开
标题翻译： pseudanabaenaceae科（蓝藻属，蓝藻属），属protolyngbya，物种protolyngbya sp。应变ITD-01
公开(公告)号：EP2465515A1
公开(公告)日：2012-06-20
申请号：EP11193982.3
申请日：2011-12-16
申请人： Associazione Termalisti Isola d'Ischia
发明人： Andreoli, Carlo , Rascio, Nicoletta , Moro, Isabella , Sciuto, Katia
IPC分类号： A61K35/74 , C12N1/20
CPC分类号： C12R1/01 , A23L33/135 , A61K35/748 , C12N1/20 , C12N9/88 , C12Y401/01039
摘要： It is herein described the identification and characterization of a new strain of cyanobacterium belonging to the family Pseudanabaenaceae (Oscillatoriales, Cyanophyta), genus Protolyngbya, designated strain ITD-01, found on the surface of the Ischia thermal muds. This cyanobacterium is found to be particularly rich in C-phycocyanin.
摘要翻译：本文描述了属于在Ischia热泥浆表面上发现的属于假鱼腥藻科（Oscillatoriales，Cyanophyta），Protolyngbya属，命名为菌株ITD-01的新菌株的鉴定和表征。发现该蓝细菌特别富含C-藻蓝蛋白。

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