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    • 5. 发明公开
    • PROCESS FOR GENERATION OF PROTEIN AND USES THEREOF
    • VERFAHREN ZUR HERSTELLUNG VON PROTEINEN UND ANWENDUNGEN DAVON
    • EP2079755A1
    • 2009-07-22
    • EP07815347.5
    • 2007-10-10
    • The Australian National University
    • GREADY, Jill, E.KANNAPPAN, Babu
    • C07K1/00C07K14/415C12N15/64C07H21/04C12N15/29
    • C12N9/88C07K1/00C07K1/13C07K1/16C07K1/18C07K1/36C07K14/415C07K2299/00C12N15/1089C12Y401/01039
    • A method of generating a protein with an improved functional property, the method comprising: (a) identifying at least one Target amino acid Residue in a first protein, wherein said Target amino acid Residue is associated with said functional property; (b) comparing at least one homologous second protein from the same or a different phylogenetic branch as the first protein with the first protein and identifying at least one Variant amino acid Residue between the first protein and the second protein; (c) selecting at least one Candidate amino acid Residue from the Variant amino acid Residue identified in (b) on the basis of said Candidate amino acid Residue affecting said Target amino acid Residue with respect to said functional property; (d) forming at least one Candidate Mutant protein in silico or producing at least one Candidate Mutant protein in vitro in which said at least one Candidate amino acid Residue from the second protein substitutes a corresponding residue in the first protein; and (e) screening said at least one Candidate Mutant protein produced in (d) to identify a protein having said improved functional property.
    • 一种产生具有改善的功能性质的蛋白质的方法,所述方法包括:(a)鉴定第一种蛋白质中的至少一种靶氨基酸残基,其中所述目标氨基酸残基与所述功能性质相关; (b)将来自与第一蛋白质相同或不同系统发生分支的至少一种同源第二蛋白质与第一蛋白质进行比较,并鉴定第一蛋白质和第二蛋白质之间的至少一个变体氨基酸残基; (c)基于所述功能性质影响所述靶氨基酸残基的所述候选氨基酸残基,从(b)中鉴定的变体氨基酸残基中选择至少一个候选氨基酸残基; (d)在体外形成至少一种候选突变体蛋白质或在体外产生至少一种候选突变体蛋白质,其中来自第二蛋白质的所述至少一个候选氨基酸残基取代第一蛋白质中相应的残基; 和(e)筛选(d)中产生的至少一种候选突变体蛋白质以鉴定具有所述改进的功能性质的蛋白质。
    • 8. 发明授权
    • PROCESS FOR GENERATION OF PROTEIN AND USES THEREOF
    • 产生蛋白质的方法及其用途
    • EP2079755B1
    • 2017-08-30
    • EP07815347.5
    • 2007-10-10
    • The Australian National University
    • GREADY, Jill, E.KANNAPPAN, Babu
    • C07K1/00C07K14/415C12N15/64C07H21/04C12N15/29
    • C12N9/88C07K1/00C07K1/13C07K1/16C07K1/18C07K1/36C07K14/415C07K2299/00C12N15/1089C12Y401/01039
    • A method of generating a protein with an improved functional property, the method comprising: (a) identifying at least one Target amino acid Residue in a first protein, wherein said Target amino acid Residue is associated with said functional property; (b) comparing at least one homologous second protein from the same or a different phylogenetic branch as the first protein with the first protein and identifying at least one Variant amino acid Residue between the first protein and the second protein; (c) selecting at least one Candidate amino acid Residue from the Variant amino acid Residue identified in (b) on the basis of said Candidate amino acid Residue affecting said Target amino acid Residue with respect to said functional property; (d) forming at least one Candidate Mutant protein in silico or producing at least one Candidate Mutant protein in vitro in which said at least one Candidate amino acid Residue from the second protein substitutes a corresponding residue in the first protein; and (e) screening said at least one Candidate Mutant protein produced in (d) to identify a protein having said improved functional property.
    • 产生具有改善的功能性质的蛋白质的方法,所述方法包括:(a)鉴定第一蛋白质中的至少一个靶氨基酸残基,其中所述靶氨基酸残基与所述功能性质相关; (b)将来自与第一蛋白质相同或不同的系统发生分支的至少一种同源第二蛋白质与第一蛋白质进行比较,并鉴定第一蛋白质与第二蛋白质之间的至少一种变体氨基酸残基; (c)基于影响所述靶氨基酸残基的所述候选氨基酸残基,根据所述功能性选择来自(b)中鉴定的变体氨基酸残基的至少一种候选氨基酸残基; (d)在计算机上形成至少一种候选突变蛋白或在体外产生至少一种候选突变蛋白,其中所述至少一种来自第二蛋白的候选氨基酸残基取代第一蛋白中的相应残基; 和(e)筛选(d)中产生的所述至少一种候选突变体蛋白质以鉴定具有所述改进的功能性质的蛋白质。