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1. 发明公开

EP3411383A1 METHODS FOR EXTRACTING PROTEINS FROM A BLOOD-BASED MATERIAL 审中-公开
公开(公告)号：EP3411383A1
公开(公告)日：2018-12-12
申请号：EP17748315.3
申请日：2017-02-03
申请人： Plasma Technologies, LLC
发明人： ZURLO, Eugene , NOWOTNIK, David Peter , HELDEBRANT, Charles , CURTIN, Dennis
IPC分类号： C07K1/30 , C07K1/16 , C07K1/22 , C07K1/34 , C07K1/36 , C07K14/81 , C07K16/06
CPC分类号： C07K1/16 , A61K35/14 , A61K38/36 , A61K38/363 , A61K38/37 , A61K38/38 , A61K38/4846 , A61K38/57 , C07K1/22 , C07K1/30 , C07K1/34 , C07K14/81 , C07K16/06
摘要： Methods of producing multiple protein products from blood-based materials including alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins are described herein. The inventive methods include steps of: salt fractionation, chromatography, ultrafiltration, diafiltration, solvent-detergent treatment, and sterile filtration. Advantageously, the inventive methods are simple and produce alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins in high yields. The sequence of process steps can be selected to obtain multiple products from various in-process materials, such as supernatants, pastes, chromatography flow-though, and chromatography washes.

2. 发明授权

EP2961761B1 PROTEIN PURIFICATION IN THE PRESENCE OF NONIONIC ORGANIC POLYMERS AT ELEVATED CONDUCTIVITY 有权
公开(公告)号：EP2961761B1
公开(公告)日：2018-09-12
申请号：EP14757178.0
申请日：2014-02-27
申请人： Agency for Science, Technology and Research
发明人： GAGNON, Peter Stanley
IPC分类号： C07K1/14 , C07K16/06 , C07K1/16 , C07K1/30 , C07K16/36
CPC分类号： C07K1/16 , C07K1/30 , C07K16/065 , C07K16/36
摘要： A method of purifying a desired protein from a preparation includes providing the preparation in a form having less than about 5% of the chromatin residing in the original production medium, contacting the preparation with a nonionic organic polymer in an amount sufficient to cause the desired protein to precipitate or adsorb on a nonionic hydrophilic surface, and adjusting a salt concentration before or during the contacting step, the adjusting step providing a sufficient salt concentration to produce a conductivity greater than physiological conductivity.

3. 发明公开

EP3118209A4 METHOD FOR PURIFYING IMMUNOGLOBULIN 有权
公开(公告)号：EP3118209A4
公开(公告)日：2017-11-08
申请号：EP14885531
申请日：2014-03-11
申请人： GREEN CROSS HOLDINGS CORP
发明人： SON KI-HWAN , KANG YONG , PARK DONG-HWAN , CHOI SUNG MIN , SEO KANG YUN , KIM KI-YONG
IPC分类号： C07K1/18 , C07K1/36 , C07K16/00 , C07K16/06
CPC分类号： C07K1/36 , C07K1/1136 , C07K1/18 , C07K1/30 , C07K1/34 , C07K16/065
摘要： The present invention relates to a method for purifying an immunoglobulin, and more particularly, to a method for purifying an immunoglobulin, which comprises: dialyzing and concentrating an immunoglobulin-containing plasma protein fraction II paste; removing thrombotic substances from the dialyzed and concentrated fraction by a purification process using ceramic cation exchange resin; and performing elution while maintaining salt concentration at a constant level to maintain the polymer content of the immunoglobulin at a low level. When the immunoglobulin purification method according to the present invention is used, the efficiency with which impurities and thrombotic substances are removed can be increased and the polymer content of the immunoglobulin can be maintained, and thus a stable immunoglobulin with improved quality can be produced.

4. 发明公开

EP3239165A1 COMPOSITION CONTAINING NITROGEN HETEROCYCLIC HEXAPEPTIDE PRECURSOR AND PREPARATION METHOD AND APPLICATION THEREOF 审中-公开
标题翻译：含有氮杂环六肽前体的组合物及其制备方法和应用
公开(公告)号：EP3239165A1
公开(公告)日：2017-11-01
申请号：EP15871972.4
申请日：2015-12-24
申请人： Shanghai Techwell Biopharmaceutical Co., Ltd
发明人： LIU, Shidong , WANG, Xiusheng , JI, Xiaoming
IPC分类号： C07K1/30 , C07K7/56
CPC分类号： C07K1/30 , A61K38/12 , A61K47/10 , A61K47/18 , C07D487/14 , C07K1/34 , C07K7/56
摘要： Disclosed is a preparation method of a composition containing a nitrogen heterocyclic hexapeptide precursor, composition A and B containing a nitrogen heterocyclic hexapeptide precursor of a compound having formula I and formula II obtained by the method, and an application of the composition used for preparing a compound having formula III.
摘要翻译：本发明公开了含有氮杂环六肽前体的组合物的制备方法，含有通过该方法获得的式I和式II化合物的氮杂环六肽前体的组合物A和B，以及用于制备化合物具有公式III。

5. 发明授权

EP2274080B1 HYDROPHOBE CELLULOSE-MEMBRAN, VERFAHREN ZU IHRER HERSTELLUNG UND IHRE VERWENDUNG IN DER HYDROPHOBEN INTERAKTIONSCHROMATOGRAPHY 有权
标题翻译：疏水纤维素膜，其制造方法相同，它们在疏水INTERAKTIONSCHROMATOGRAPHY
公开(公告)号：EP2274080B1
公开(公告)日：2017-10-18
申请号：EP09731848.9
申请日：2009-02-10
申请人： Sartorius Stedim Biotech GmbH
发明人： FABER, René
IPC分类号： B01D69/02 , B01D71/10 , B01D67/00 , B01J20/28 , B01J20/26 , B01D15/32 , B01J20/285 , C07K1/30 , C08B15/10
CPC分类号： B01D67/0093 , B01D15/327 , B01D69/02 , B01D71/10 , B01D2323/16 , B01D2323/30 , B01D2323/36 , B01D2325/02 , B01D2325/12 , B01D2325/14 , B01D2325/16 , B01J20/262 , B01J20/265 , B01J20/267 , B01J20/28033 , B01J20/28078 , B01J20/285 , C07K1/30 , C08B15/10

6. 发明授权

EP2890710B1 METHODS FOR PURIFICATION OF ANTIBODIES USING ALIPHATIC ALCOHOLS 有权
公开(公告)号：EP2890710B1
公开(公告)日：2017-08-09
申请号：EP13753180.2
申请日：2013-08-27
申请人： Novartis AG
发明人： JUNGBAUER, Alois , SATZER, Peter , TSCHELIESSNIG, Anne-Luise
IPC分类号： C07K16/06 , C07K1/30
CPC分类号： C07K16/00 , C07K1/30 , C07K16/065

7. 发明公开

EP3177712A1 METHODS FOR THE ISOLATION OF EXTRACELLULAR VESICLES AND OTHER BIOPARTICLES FROM URINE AND OTHER BIOFLUIDS 审中-公开
标题翻译：方法细胞外微囊的和尿液和其它生物体液中的其他生物粒子
公开(公告)号：EP3177712A1
公开(公告)日：2017-06-14
申请号：EP15830435.2
申请日：2015-08-05
申请人： Ymir Genomics LLC
发明人： PENDERGRAST, P. Shannon , PENDERGRAST, R. Scott , PENDERGRAST, J. Stephen , MARKOWSKA, Anna Irmina
IPC分类号： C12N5/07 , B01D9/02 , B01D21/26 , C07K1/30 , C07K14/705
CPC分类号： B01D9/0054 , B01D21/26 , B01D21/262 , B01D2009/0086 , C07K1/30 , C07K1/303 , C07K1/306 , C07K14/705 , C12N15/1003 , G01N1/4055
摘要： Compositions and methods for the isolation of protein-nucleic acid complexes and microvesicles (collectively referred to as “bioparticles”) released by mammalian cells into body fluids or cell culture media are provided. Isolated bioparticles of the invention contain biological molecules that are useful as diagnostic/prognostic biomarkers or for identification of therapeutic targets (e.g., disease or disorder-associated miRNAs). The isolation of biological molecules as described herein results in purification and concentration of the molecules. Methods for producing bio fluids that are free of detectable bioparticles, that are largely depleted of bioparticles, or that possess a reduced concentration of bioparticles compared to a bio fluid starting material (collectively termed “bioparticle-depleted”) are also provided. Isolation of bioparticle-depleted biofluid is useful, e.g., in experimental systems where it is desirable to use a biofluid that does not contain endogenous bioparticles, or has been substantially depleted of endogenous bioparticles, from the source material.
摘要翻译：组合物和蛋白质 - 核酸复合物和微泡的分离方法（通过对统称为“生物颗粒”）由哺乳动物细胞释放到体液或细胞培养基中提供。本发明的分离的生物颗粒包含生物分子确实可用作诊断/预后生物标记物或用于治疗靶标（例如，疾病或病症相关的miRNA）的识别是有用的。生物分子的分离如在分子的纯化和浓缩的结果进行说明。用于生产生物流体的方法那样被自由可检测生物颗粒的，并在很大程度上被耗尽生物颗粒，或确实拥有相较于生物流体原料（统称为“生物颗粒贫化”）被提供，因此生物颗粒的浓度降低。生物颗粒耗尽生物流体的分离是有用的，e.g“在实验系统中，其中需要使用生物流体并不含有内源性生物颗粒，或已被基本耗尽的内源性生物颗粒的，从源材料。

8. 发明公开

EP3110829A1 ANTIBODY PURIFICATION PROCESS 审中-公开
标题翻译： ANTIKÖRPERREINIGUNGSVERFAHREN
公开(公告)号：EP3110829A1
公开(公告)日：2017-01-04
申请号：EP14883697.6
申请日：2014-02-27
申请人： Agency for Science, Technology and Research
发明人： GAGNON, Peter Stanley
IPC分类号： C07K1/14
CPC分类号： C07K1/30 , C07K1/32 , C07K16/00
摘要： A method of purifying a target antibody includes contacting a cell culture harvest or a protein preparation including at least one target antibody with at least one fatty acid having 7 to 10 carbon atoms to form a mixture, contacting this mixture with allantoin, and then separating solid materials to provide a solution comprising the target antibody. Solid materials can be removed by filtration, sedimentation or centrifugation, and the fatty acids can be enanthic, caprylic, pelargonic, nonenoic or capric acid. The invention is also directed to kits used to facilitate this method of antibody purification.
摘要翻译：纯化靶抗体的方法包括将细胞培养物收获物或包含至少一种靶抗体的蛋白质制剂与至少一种具有7至10个碳原子的脂肪酸接触以形成混合物，使该混合物与尿囊素接触，然后分离固体提供包含靶抗体的溶液的材料。固体材料可以通过过滤，沉淀或离心去除，脂肪酸可以是甘油，辛酸，壬酸，壬烯或癸酸。本发明还涉及用于促进抗体纯化方法的试剂盒。

9. 发明公开

EP3088412A1 PROTEIN PURIFICATION METHOD 审中-公开
标题翻译： PROTEINAUFREINIGUNGSVERFAHREN
公开(公告)号：EP3088412A1
公开(公告)日：2016-11-02
申请号：EP16170137.0
申请日：2002-03-11
申请人： CHUGAI SEIYAKU KABUSHIKI KAISHA
发明人： TAKEDA, Kozo , OCHI, Norimichi
IPC分类号： C07K1/30 , C07K1/22 , C07K16/00 , C12P21/08 , A61K39/395
CPC分类号： C07K1/30
摘要： A method for removing contaminant DNA in a sample containing a physiologically active protein, which comprises the following steps:
1) converting the sample containing a physiologically active protein into a neutral aqueous solution of low conductivity; and
2) removing the resulting particles.
摘要翻译：一种除去含有生理活性蛋白质的样品中的污染物DNA的方法，其包括以下步骤：1）将含有生理活性蛋白质的样品转化为低电导率的中性水溶液; 和2）去除所得颗粒。

10. 发明公开

EP3071590A1 METHODS FOR STRUCTURAL DETERMINATION OF SELENIUM DERIVATIZED NUCLEIC ACID COMPLEXES 审中-公开
标题翻译： VERFAHREN ZUR STRUKTURBESTIMMUNG VON SELENDERIVATISITENTENNUKLEINSÄUREKOMPLEXEN
公开(公告)号：EP3071590A1
公开(公告)日：2016-09-28
申请号：EP14863946.1
申请日：2014-11-21
申请人： SeNA Research, Inc.
发明人： HUANG, Zhen
IPC分类号： C07K1/14 , C07K1/30 , G01N33/53 , G01N33/68
CPC分类号： A61K47/4823 , A61K38/465 , A61K47/61 , C07K1/30 , C12N9/22 , C12N9/96 , C12Y301/26004 , G01N33/5308 , G06F19/00 , G16H50/50 , Y02A90/26
摘要： Methods for crystallizing a molecule of interest, such as a polypeptide, in complex with nucleic acid, including contacting the molecule of interest with selenium-derivatized nucleic acid and crystallizing the molecule of interest/selenium-derivatized nucleic acid complex are provided. Methods for determining the X-ray crystal structure of molecule of interest/selenium-derivatized nucleic acid complexes are also provided. Typically, the method of X-ray crystal structural determination includes selenium single-wavelength anomalous phasing of the selenium-derivatized nucleic acid. In some embodiments the phases for the X-ray crystal structure of the molecule of interest are not provided from another crystal. Also disclosed are methods of affecting a biological process by administering a functional nucleic acid to a cell or a subject and/or by bringing into contact a nuclease and a functional nucleic acid, where the functional nucleic acid is selenium-derivatized nucleic acid.
摘要翻译：提供了与核酸复合的目标分子（例如多肽）结晶的方法，包括将感兴趣的分子与硒衍生的核酸接触并结晶感兴趣的分子/硒衍生的核酸复合物。还提供了用于确定目的分子的X射线晶体结构/硒衍生的核酸复合物的方法。通常，X射线晶体结构测定的方法包括硒衍生化核酸的硒单波长异常定相。在一些实施方案中，感兴趣的分子的X射线晶体结构的相不是从另一晶体提供的。还公开了通过向细胞或受试者施用功能性核酸和/或通过使功能性核酸为硒衍生化的核酸与核酸酶和功能性核酸接触来影响生物学过程的方法。

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