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1. 发明授权

EP3004385B1 NANOPORE-BASED NUCLEIC ACID ANALYSIS WITH MIXED FRET DETECTION 有权转让
公开(公告)号：EP3004385B1
公开(公告)日：2018-11-28
申请号：EP14801275.0
申请日：2014-05-23
申请人： Quantapore Inc.
发明人： HUBER, Martin , CLANCY, Bason E. , HARDENBOL, Paul
IPC分类号： C12Q1/6869
CPC分类号： C12Q1/6869 , C12Q2565/101 , C12Q2565/631
摘要： Various methods, systems and devices for optical detection and analysis of polymers, such as polynucleotides, using nanopores, e.g., for determining sequences of nucleic acids, are provided herein. In certain variations, methods and systems for determining a nucleotide sequence of a polynucleotide, which include measuring mixed FRET signals as a polynucleotide translocates through a nanopore and determining a nucleotide sequence of the polynucleotide from the mixed FRET signals, are provided.

2. 发明公开

EP3347714A1 METHODS AND SYSTEMS FOR MULTIPLEX QUANTITATIVE NUCLEIC ACID AMPLIFICATION 有权
公开(公告)号：EP3347714A1
公开(公告)日：2018-07-18
申请号：EP15903729.0
申请日：2015-09-10
申请人： InSilixa Inc.
发明人： HASSIBI, Arjang , JIRAGE, Kshama , MANICKAM, Arun , SINGH, Rituraj
IPC分类号： G01N33/53
CPC分类号： C12Q1/6851 , C12Q2537/143 , C12Q2565/101 , C12Q2565/514 , C12Q2565/607
摘要： The present disclosure provides methods, devices and systems that enable simultaneous multiplexing amplification reaction and real-time detection in a single reaction chamber. An aspect of the present disclosure provides a method for assaying a presence or absence of at least one target nucleic acid molecule. In some embodiments of aspects provided herein, at least one signal is produced upon binding of probes to the target nucleic acid molecule and/or the limiting primer. In some embodiments of aspects provided herein, a reaction mixture comprises a plurality of template nucleic acid molecules and the probes specifically bind to a plurality of target nucleic molecules as amplification products of the plurality of template nucleic acid molecules.

3. 发明公开

EP3274094A1 MULTIPLEXED ANALYSIS OF NUCLEIC ACID HYBRIDIZATION THERMODYNAMICS USING INTEGRATED ARRAYS 审中-公开
标题翻译：用集成阵列对核酸杂交热力学进行多重分析
公开(公告)号：EP3274094A1
公开(公告)日：2018-01-31
申请号：EP16769558.4
申请日：2016-03-22
申请人： InSilixa Inc.
发明人： HASSIBI, Arjang , JIRAGE, Kshama , MANICKAM,Arun , MILANINIA, Kaveh
IPC分类号： B01L3/00 , C12Q1/68 , C40B30/04 , G01N33/48 , H01L21/302
CPC分类号： C12Q1/6837 , C12Q2527/107 , C12Q2565/101 , C12Q2565/607
摘要： The present disclosure provides methods and devices for simultaneous identification of a plurality of target nucleic acid sequences in a single sample chamber that includes an addressable array of nucleic acid probes attached to a solid surface. Addressable signals can be generated and measured, in real-time, upon hybridization of target sequences at the individual probe locations within the array while the temperature of the system is varied. Such generated signals, as a function temperature, can then be used to compute the properties of nucleic acid hybridization at each addressable location which is ultimately utilized to estimate the sequence of the target nucleic acids. In particular, an integrated semiconductor biosensor array device can be used to measure the addressable signals.

4. 发明公开

EP3250711A1 METHOD AND PRODUCT FOR PREVENTING FALSE POSITIVES IN METHODS EMPLOYING DDNTP'S 审中-公开
标题翻译：用于预防使用DDNTP的方法中的假阳性的方法和产品
公开(公告)号：EP3250711A1
公开(公告)日：2017-12-06
申请号：EP16702421.5
申请日：2016-02-01
申请人： Norwegian University Of Life Sciences , Hedmark University Of Applied Sciences
发明人： RUDI, Knut , WILSON, Robert C.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6816 , C12Q1/6818 , C12Q1/6848 , C12Q2535/101 , C12Q2521/537 , C12Q2537/143 , C12Q2565/101
摘要： The present invention relates to a method which prevents undesirable binding of ddNTPs to double stranded polynucleotides when in the presence of a polymerase. Such methods may be used to prevent the appearance of false positives in methods employing ddNTPs, e.g. in sequence detection methods. The present invention also provides a method of avoiding a false Tm reading or false FRET effects (such as false positive quenching), for example in a melting curve analysis method. In particular a method is provided in which a target nucleotide sequence in a test polynucleotide is detected using a method in which a double stranded molecule is generated which may or may not comprise two labels depending on whether the target sequence is present in which the presence of the two labels is determined, preferably by performing a melting curve analysis.

5. 发明授权

EP2814986B1 METHOD FOR RELATIVE QUANTIFICATION OF NUCLEIC ACID SEQUENCE, EXPRESSION, OR COPY CHANGES, USING COMBINED NUCLEASE, LIGATION, AND POLYMERASE REACTIONS 有权
标题翻译：用于核酸序列，表达或复制变化的相对定量的方法，使用组合核酸酶，连接和聚合酶反应
公开(公告)号：EP2814986B1
公开(公告)日：2017-10-25
申请号：EP13749841.6
申请日：2013-02-14
申请人： Cornell University
发明人： BARANY, Francis , SPIER, Eugene , MIR, Alain
IPC分类号： C12Q1/68
CPC分类号： G01N33/5308 , C12Q1/6806 , C12Q1/6813 , C12Q1/6851 , C12Q1/6853 , C12Q1/6855 , C12Q2521/501 , C12Q2525/00 , C12Q2525/301 , C12Q2533/107 , C12Q2521/319 , C12Q2525/113 , C12Q2525/161 , C12Q2565/101 , C12Q2565/514 , C12Q2521/301
摘要： The present invention is directed to methods for identifying the presence of one or more target nucleotide sequences in a sample that involve a nuclease-ligation reaction. In some embodiments, the ligation products formed in the nuclease-ligation process of the present invention are subsequently amplified using a polymerase chain reaction. The ligated product sequences or extension products thereof are detected, and the presence of one or more target nucleotide sequences in the sample is identified based on the detection.

6. 发明公开

EP3093342A4 RNA MICRO-ARRAY FOR DETECTING INTERACTION BETWEEN PROTEIN AND CONFORMATION-CONTAINING RNA 有权
标题翻译： RNA-MIKROARRAYFÜRDEN NACHWEIS EINER WECHSELWIRKUNG ZWISCHEN PROTEINEN UND KONFORMATIONSHALTIGER RNA
公开(公告)号：EP3093342A4
公开(公告)日：2017-09-20
申请号：EP15734866
申请日：2015-01-09
申请人： UNIV KYOTO
发明人： SAITO HIROHIDE , TAYA TOSHIKI , KASHIDA SHUNICHI
IPC分类号： C12N15/09 , C12M1/00 , C12Q1/68 , G01N37/00
CPC分类号： C12Q1/6816 , C12Q1/6806 , C12Q1/6811 , C12Q1/6818 , C12Q1/6834 , C12Q2525/207 , C12Q2525/301 , C12Q2563/179 , C12Q2565/101 , C12Q2565/514 , C12Q2565/549 , C12Q2525/205 , C12Q2565/501
摘要： Interaction with a protein is detected by using an RNA probe containing the following sequences; (i) a complementary strand sequence to a DNA barcode sequence, (ii) a sequence of a first stem portion, (iii) a sequence of a second stem portion complementary to the first stem portion for hybridizing with the first stem portion to form a double-stranded stem, and (iv) a sequence of a loop portion contained in RNA for linking the first and second stem portions.
摘要翻译：通过使用含有以下序列的RNA探针检测与蛋白质的相互作用; （i）DNA条形码序列的互补链序列，（ii）第一茎部分的序列，（iii）与第一茎部分互补的第二茎部分的序列，用于与第一茎部分杂交以形成（iv）包含在用于连接第一和第二茎部分的RNA中的环部分的序列。

7. 发明授权

EP1344835B1 REAL-TIME NUCLEIC ACID DETECTION PROCESSES AND COMPOSITIONS 有权
标题翻译：实时核酸检测过程和组合物
公开(公告)号：EP1344835B1
公开(公告)日：2017-06-14
申请号：EP03004895.3
申请日：2003-03-06
申请人： Enzo Life Sciences, Inc.
发明人： RABBANI, Elazar , STAVRIANOPOULOS, Jannis G. , DONEGAN, James J. , COLEMAN, Jack , LIU, Dakai
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q1/6834 , C12Q1/6844 , C12Q1/6853 , C12Q1/686 , G01N21/64 , G01N21/6486 , C12Q2565/101 , C12Q2533/101 , C12Q2561/113

8. 发明公开

EP3141615A1 REAL-TIME NUCLEIC ACID DETECTION PROCESSES AND COMPOSITIONS 审中-公开
标题翻译： VERFAHREN ZUR ECHTZEITERKENNUNG VONNUKLEINSÄURENUND ZUSAMMENSETZUNGEN
公开(公告)号：EP3141615A1
公开(公告)日：2017-03-15
申请号：EP16188027.3
申请日：2003-03-06
申请人： Enzo Life Sciences, Inc.
发明人： RABBANI, Elazar , STAVRIANOPOULOS, Jannis G. , DONEGAN, James J. , COLEMAN, Jack , LIU, Dakai
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q1/6834 , C12Q1/6844 , C12Q1/6853 , C12Q1/686 , G01N21/64 , G01N21/6486 , C12Q2565/101 , C12Q2533/101 , C12Q2561/113
摘要： The present invention provides a composition of matter comprising: (a) a first part comprising at least one first nucleic acid primer that comprises (i) at least one first energy transfer element; and (ii) a nucleic acid sequence which is complementary to a nucleic acid sequence in at least a portion of a nucleic acid of interest; (b) a second part comprising at least one second nucleic acid primer that comprises: (i) at least one second energy transfer element; and (ii) a nucleic acid sequence which is identical to a nucleic acid sequence in at least a portion of said nucleic acid of interest; wherein said first nucleic acid primer does not comprise said second energy transfer element, and wherein said second nucleic acid primer does not comprise said first energy transfer element; wherein the first energy transfer element is capable of acting as an energy donor and the second energy transfer element is capable of acting as an energy acceptor, or the first energy transfer element is capable of acting as acting as an energy acceptor and the second energy transfer element is capable of acting as an energy donor; and wherein the sequences of the first nucleic acid primer and the second nucleic acid primer are selected such that if the nucleic acid of interest is present in the sample, said first and second nucleic acid primers will be incorporated into different complementary strands of a double stranded amplicon in sufficient proximity for energy transfer to occur between the first energy transfer element and the second energy transfer element of the incorporated primers; (c) a sample suspected of containing said nucleic acid of interest; and (d) reagents for carrying out nucleic acid strand extension.
摘要翻译：本发明提供一种物质组合物，其包含：（a）第一部分，其包含至少一个第一核酸引物，其包含（i）至少一种第一能量转移元件; 和（ii）与感兴趣的核酸的至少一部分中的核酸序列互补的核酸序列; （b）第二部分，其包含至少一个第二核酸引物，其包含：（i）至少一个第二能量转移元件; 和（ii）与目的核酸的至少一部分中的核酸序列相同的核酸序列; 其中所述第一核酸引物不包含所述第二能量转移元件，并且其中所述第二核酸引物不包含所述第一能量转移元件; 其中所述第一能量传递元件能够用作能量供体，并且所述第二能量传递元件能够用作能量受体，或者所述第一能量传递元件能够用作能量受体并且所述第二能量传递元件元素能够作为能量供体; 选择第一核酸引物和第二核酸引物的序列，使得如果目标核酸存在于样品中，则所述第一和第二核酸引物将被并入双链的不同互补链扩增子足够接近于能量转移发生在第一能量转移元件和引入的引物的第二能量转移元件之间; （c）怀疑含有所述目的核酸的样品; 和（d）用于进行核酸链延伸的试剂。

9. 发明公开

EP3098322A1 METHOD TO DETECT ACTIVITY OF A POLYMERASE 审中-公开
标题翻译： VERFAHREN ZUR ERKENNUNG DERAKTIVITÄTEINES POLYMERASE
公开(公告)号：EP3098322A1
公开(公告)日：2016-11-30
申请号：EP15169966.7
申请日：2015-05-29
申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. , Lead Discovery Center GmbH
发明人： BERGBREDE, Tim , LARSSON, Nils-Göran , GUSTAFSSON, Claes , FALKENBERG-GUSTAFSSON, Maria
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q1/48 , C12Q1/68 , C12Q1/6825 , G01N33/542 , G01N33/582 , G01N2333/91245 , C12Q2521/119 , C12Q2565/101
摘要： The present invention relates to methods for detection of nucleotide polymerase activity and methods of detecting compounds that modulate nucleotide polymerase activity, by detecting product formation of the nucleotide polymerase to be tested based on determination of close proximity of two labeled nucleotide probes able to bind the product of the nucleotide polymerase. It is preferred that proximity dependent energy transfer, such as förster resonance energy transfer, between said labeled nucleotide probes is determined. The invention further provides kits comprising components for carrying out the inventive methods for detection of nucleotide polymerase activity.
摘要翻译：本发明涉及用于检测核苷酸聚合酶活性的方法和检测调节核苷酸聚合酶活性的化合物的方法，通过基于能够结合产物的两个标记的核苷酸探针的紧密度的测定来检测待测试的核苷酸聚合酶的产物形成的核苷酸聚合酶。优选确定所述标记的核苷酸探针之间的接近依赖的能量转移，例如förster共振能量转移。本发明还提供了包含用于实施本发明的用于检测核苷酸聚合酶活性的方法的组分的试剂盒。

10. 发明授权

EP1746172B1 Asymmetric PCR coupled with post-PCR characterization for the identification of nucleic acids 有权
标题翻译：用PCR后表征用于核酸的标识相关联的非对称PCR
公开(公告)号：EP1746172B1
公开(公告)日：2016-10-05
申请号：EP06013329.5
申请日：2006-06-28
申请人： Roche Diagnostics GmbH , F. Hoffmann-La Roche AG
发明人： Newton, Nicolas , Will, Stephen Gordon
IPC分类号： C12Q1/68 , C12Q1/70
CPC分类号： C12Q1/6818 , C12Q1/6823 , C12Q1/6851 , C12Q1/6858 , C12Q1/6883 , C12Q1/707 , Y10S435/975 , C12Q2565/101 , C12Q2563/173 , C12Q2527/107 , C12Q2561/113 , C12Q2531/107 , C12Q2561/101 , C12Q2523/319

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