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2. 发明公开

EP3141615A1 REAL-TIME NUCLEIC ACID DETECTION PROCESSES AND COMPOSITIONS 审中-公开
标题翻译： VERFAHREN ZUR ECHTZEITERKENNUNG VONNUKLEINSÄURENUND ZUSAMMENSETZUNGEN
公开(公告)号：EP3141615A1
公开(公告)日：2017-03-15
申请号：EP16188027.3
申请日：2003-03-06
申请人： Enzo Life Sciences, Inc.
发明人： RABBANI, Elazar , STAVRIANOPOULOS, Jannis G. , DONEGAN, James J. , COLEMAN, Jack , LIU, Dakai
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q1/6834 , C12Q1/6844 , C12Q1/6853 , C12Q1/686 , G01N21/64 , G01N21/6486 , C12Q2565/101 , C12Q2533/101 , C12Q2561/113
摘要： The present invention provides a composition of matter comprising: (a) a first part comprising at least one first nucleic acid primer that comprises (i) at least one first energy transfer element; and (ii) a nucleic acid sequence which is complementary to a nucleic acid sequence in at least a portion of a nucleic acid of interest; (b) a second part comprising at least one second nucleic acid primer that comprises: (i) at least one second energy transfer element; and (ii) a nucleic acid sequence which is identical to a nucleic acid sequence in at least a portion of said nucleic acid of interest; wherein said first nucleic acid primer does not comprise said second energy transfer element, and wherein said second nucleic acid primer does not comprise said first energy transfer element; wherein the first energy transfer element is capable of acting as an energy donor and the second energy transfer element is capable of acting as an energy acceptor, or the first energy transfer element is capable of acting as acting as an energy acceptor and the second energy transfer element is capable of acting as an energy donor; and wherein the sequences of the first nucleic acid primer and the second nucleic acid primer are selected such that if the nucleic acid of interest is present in the sample, said first and second nucleic acid primers will be incorporated into different complementary strands of a double stranded amplicon in sufficient proximity for energy transfer to occur between the first energy transfer element and the second energy transfer element of the incorporated primers; (c) a sample suspected of containing said nucleic acid of interest; and (d) reagents for carrying out nucleic acid strand extension.
摘要翻译：本发明提供一种物质组合物，其包含：（a）第一部分，其包含至少一个第一核酸引物，其包含（i）至少一种第一能量转移元件; 和（ii）与感兴趣的核酸的至少一部分中的核酸序列互补的核酸序列; （b）第二部分，其包含至少一个第二核酸引物，其包含：（i）至少一个第二能量转移元件; 和（ii）与目的核酸的至少一部分中的核酸序列相同的核酸序列; 其中所述第一核酸引物不包含所述第二能量转移元件，并且其中所述第二核酸引物不包含所述第一能量转移元件; 其中所述第一能量传递元件能够用作能量供体，并且所述第二能量传递元件能够用作能量受体，或者所述第一能量传递元件能够用作能量受体并且所述第二能量传递元件元素能够作为能量供体; 选择第一核酸引物和第二核酸引物的序列，使得如果目标核酸存在于样品中，则所述第一和第二核酸引物将被并入双链的不同互补链扩增子足够接近于能量转移发生在第一能量转移元件和引入的引物的第二能量转移元件之间; （c）怀疑含有所述目的核酸的样品; 和（d）用于进行核酸链延伸的试剂。

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