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1. 发明授权

EP2893037B1 NUCLEOTIDES AND PRIMERS WITH REMOVABLE BLOCKING GROUPS 有权
标题翻译：具有可移除封闭基团的核苷酸和引物
公开(公告)号：EP2893037B1
公开(公告)日：2018-04-25
申请号：EP13753051.5
申请日：2013-08-15
申请人： Illumina, Inc.
发明人： CHEN, Cheng-Yao , PADMABANDU, Gothami
IPC分类号： C12Q1/68
CPC分类号： C12P19/34 , C12Q1/6848 , C12Q2523/107 , C12Q2525/186 , C12Q2563/137 , C12Q2549/101
摘要： Provided herein is a method of amplifying nucleic acids using a plurality of modified nucleotides one or more of the nucleotides comprising a 3′ blocking group. Also provided is a method of amplifying nucleic acids using oligonucleotide primers one or both of the primers comprising a 3′ blocking group on one or more of the nucleotides of the primers.

2. 发明授权

EP2603608B1 IMPROVED METHOD FOR ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS 有权
标题翻译：改进方法的核酸等温扩增
公开(公告)号：EP2603608B1
公开(公告)日：2016-09-28
申请号：EP11751567.6
申请日：2011-08-09
申请人： Qiagen GmbH
发明人： KORFHAGE, Christian
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12N11/04 , C12P19/34 , C12Q1/6848 , C12Q2547/107 , C12Q2527/125 , C12Q2527/107 , C12Q2531/119 , C12Q2549/101

3. 发明公开

EP2828403A1 MODIFIED RNASE H ENZYMES AND THEIR USES 审中-公开
标题翻译：改进的RNA酶-H酶及其用途
公开(公告)号：EP2828403A1
公开(公告)日：2015-01-28
申请号：EP13713666.9
申请日：2013-03-15
申请人： Integrated DNA Technologies Inc.
发明人： WALDER, Joseph, Alan , BEHLKE, Mark, Aaron , ROSE, Scott, D. , DOBOSY, Joseph , RUPP, Susan, Marie
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6848 , C12N9/22 , C12N9/99 , C12Y301/26004 , C12Q2521/107 , C12Q2521/327 , C12Q2525/186 , C12Q2549/101
摘要： The invention provides a provides improvements to assays that employ RNase H cleavage for biological applications related to nucleic acid amplification and detection, where the RNase H has been reversibly inactivated.

4. 发明授权

EP2625284B1 METHOD FOR CELL LYSIS IN A RT-PCR REACTION BUFFER 有权
标题翻译：程序用于在细胞裂解RT-PCR反应缓冲液
公开(公告)号：EP2625284B1
公开(公告)日：2015-01-28
申请号：EP11763703.3
申请日：2011-09-30
申请人： Roche Diagnostics GmbH , F.Hoffmann-La Roche AG
发明人： HOFFMANN, Ingrid , WALCH, Heiko
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , C12Q2561/113 , C12Q2549/101 , C12Q2547/101 , C12Q2521/107

5. 发明公开

EP2644707A1 RNase-H-based assays utilizing modified RNA monomers 有权
标题翻译： RNase-H basierte测定Verwendung von RNA Monomeren
公开(公告)号：EP2644707A1
公开(公告)日：2013-10-02
申请号：EP13173388.3
申请日：2009-04-30
申请人： Integrated Dna Technologies, Inc.
发明人： Walder, Joseph Alan , Behlke, Mark Aaron , Rose, Scott , Dobosy, Joseph
IPC分类号： C12Q1/68
CPC分类号： C12N9/96 , C12Q1/6844 , C12Q1/6853 , C12Q2549/101 , C12Q2525/186 , C12Q2521/301 , C12Q2525/121
摘要： The present invention pertains to novel oligonucleotide compounds for use in various biological assays, such as nucleic acid amplification, ligation and sequencing reactions. The novel oligonucleotides comprise a ribonucleic acid domain and a blocking group at or near the 3' end of the oligonucleotide. These compounds offer an added level of specificity previously unseen. Methods for performing nucleic acid amplification, ligation and sequencing are also provided. Additionally, kits containing the oligonucleotides are also disclosed herein.
摘要翻译：本发明涉及用于各种生物测定的新型寡核苷酸化合物，例如核酸扩增，连接和测序反应。该寡核苷酸在寡核苷酸的3'末端或其附近包含核糖核酸结构域和封闭基团。这些化合物提供了前所未见的增加的特异性水平。还提供了进行核酸扩增，连接和测序的方法。此外，本文还公开了含有寡核苷酸的试剂盒。

6. 发明公开

EP2551356A4 METHOD FOR DETECTING TARGET BASE SEQUENCE USING COMPETITIVE PRIMER 有权
标题翻译： VERFAHREN ZUR ERKENNUNG VON ZIELBASISSEQUENZEN MITHILFE KONKURRIERENDER PRIMER
公开(公告)号：EP2551356A4
公开(公告)日：2013-09-11
申请号：EP11759408
申请日：2011-03-23
申请人： TOPPAN PRINTING CO LTD
发明人： MAKINO YOICHI , YAMANE AKIO , NAKAYAMA MASATO
IPC分类号： C12Q1/68 , C12N15/09
CPC分类号： C12Q1/6869 , C12Q1/6827 , C12Q1/6858 , C12Q2531/119 , C12Q2537/161 , C12Q2531/143 , C12Q2531/113 , C12Q2525/161 , C12Q2525/185 , C12Q2525/204 , C12Q2535/125 , C12Q2549/101 , C12Q2563/107 , C12Q2565/101
摘要： Disclosed is a method of detecting a target base sequence having a polymorphic base, the method including: (a) a step of adding to a nucleic acid sample having a target nucleic acid that includes a base sequence including the target base sequence: at least one type of detection primer, at least one type of competitive primer, and at least one type of common primer; (b) a step of annealing the detection primer and the competitive primer to the target nucleic acid in a competitive manner, thereby synthesizing an extension product A; (c) a step of annealing the common primer to the extension product A obtained in the step (b) or in the following step (d), thereby synthesizing an extension product B; (d) a step of annealing the detection primer or the competitive primer to the extension product B obtained in the previous step (c), thereby synthesizing the extension product A; and (e) a step of detecting the extension product A or the extension product B.
摘要翻译：公开了一种检测具有多态性碱基的靶碱基序列的方法，该方法包括：（a）向具有靶核酸的核酸样品添加步骤，所述靶核酸包括含有靶碱基序列的碱基序列：至少一个类型的检测引物，至少一种类型的竞争性引物和至少一种类型的通用引物; （b）以竞争的方式将检测用引物和竞争性引物与靶核酸退火而合成延伸产物A的工序; （c）将通用引物退火至在步骤（b）或在随后的步骤（d）中获得的延伸产物A的步骤，由此合成延伸产物B; （d）将检测用引物或竞争性引物退火至前一步骤（c）中获得的延伸产物B，从而合成延伸产物A的步骤; 和（e）检测延伸产品A或延伸产品B的步骤。

7. 发明公开

EP2625284A1 METHOD FOR CELL LYSIS IN A RT-PCR REACTION BUFFER 有权
标题翻译：程序用于在细胞裂解RT-PCR反应缓冲液
公开(公告)号：EP2625284A1
公开(公告)日：2013-08-14
申请号：EP11763703.3
申请日：2011-09-30
申请人： Roche Diagnostics GmbH , F.Hoffmann-La Roche AG
发明人： HOFFMANN, Ingrid , WALCH, Heiko
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , C12Q2561/113 , C12Q2549/101 , C12Q2547/101 , C12Q2521/107
摘要： The present invention in general provides a method for amplification of a target RNA, comprising the steps of (i) transfering a liquid with a first volume comprising at least one or more living cells into a vessel (ii) adding to said vessel an RT-PCR reaction buffer with a second volume, whereas said second volume is at least 2x as large as said first volume (iii) lysing said at least one or more living cells within said vessel by means of incubation for at least 20 seconds at at least 90°C, and (iv) amplifying said target by means of a one-step RT-PCR without performance of an intermediate purification step.

8. 发明公开

EP2418286A1 Improved method for isothermal amplification of nucleic acids 审中-公开
标题翻译： Verbeetes Verfahren zur isothermalen Amplifikation vonNukleinsäuren
公开(公告)号：EP2418286A1
公开(公告)日：2012-02-15
申请号：EP10172400.3
申请日：2010-08-10
申请人： QIAGEN GmbH
发明人： Korfhage, Christian
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12N11/04 , C12P19/34 , C12Q1/6848 , C12Q2547/107 , C12Q2527/125 , C12Q2527/107 , C12Q2531/119 , C12Q2549/101
摘要： A method is disclosed for improved isothermal amplification of nucleic acids comprising the step of release of an essential component from a matrix under predetermined conditions. Furthermore, the invention relates to a kit comprising mesophilic enzyme and a matrix with embedded essential components for isothermal amplification. A composition comprising a matrix and a mesophilic enzyme and a method for embedding a mesophilic enzyme are disclosed as well.
摘要翻译：公开了一种用于改进核酸等温扩增的方法，包括在预定条件下从基质中释放必需组分的步骤。此外，本发明涉及包含嗜温酶和具有用于等温扩增的嵌入必需成分的基质的试剂盒。还公开了包含基质和嗜温酶的组合物和用于包埋嗜温酶的方法。

9. 发明公开

EP2308990A1 Analyzing messenger RNA and micro RNA in the same reaction mixture 有权
标题翻译：分析von Boten-RNA和Mikro-RNA im gleichen Reaktionsansatz
公开(公告)号：EP2308990A1
公开(公告)日：2011-04-13
申请号：EP10191745.8
申请日：2006-07-17
申请人： Life Technologies Corporation
发明人： Lao, Kai Qin , Straus, Neil A.
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6809 , C12Q1/6844 , C12Q1/6848 , C12Q1/6853 , C12Q2549/101 , C12Q2537/143 , C12Q2527/107 , C12Q2525/301 , C12Q2525/207 , C12Q2533/101
摘要： The present teachings provide methods, compositions, and kits for performing primer extension reactions on at least two target polynucleotides in the same reaction mixture. In some embodiments, a reverse transcription reaction is performed on a first target polynucleotide with a hot start primer comprising a self-complementary stem and a loop, and extension products form at high temperatures but extension products form less so at low temperatures since the self-complementary stem of the hot start primer prevents hybridization of the target specific region to the target. However, non-hot start primers with free target specific regions can hybridize to their corresponding targets at the low temperature and extension can happen at the low temperature.
摘要翻译：本教导提供了用于在相同反应混合物中的至少两个靶多核苷酸上进行引物延伸反应的方法，组合物和试剂盒。在一些实施方案中，对具有包含自身互补茎和环的热起始底物的第一靶多核苷酸进行逆转录反应，并且在高温下形成延伸产物，但是延伸产物在低温下形成较少，热启动引物的互补干扰物可防止目标特异性区域与靶标的杂交。然而，具有游离目标特异性区域的非热启动引物可以在低温下与其相应的靶标杂交，并且延伸可以在低温下发生。

10. 发明公开

EP2241635A1 Nucleic acid ligand inhibitors to DNA polymerases 审中-公开
标题翻译： Nukleinsäure-配体抑制基因DNA聚合酶
公开(公告)号：EP2241635A1
公开(公告)日：2010-10-20
申请号：EP10007279.2
申请日：2000-02-24
申请人： Gilead Sciences, Inc.
发明人： Jayasena, Sumedha , Gold, Larry
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04 , C07H21/02
CPC分类号： C07H21/00 , C12N15/115 , C12N2310/16 , C12N2310/321 , C12Q1/6848 , G01N2333/96433 , C12Q2549/101 , C12Q2525/205 , C12N2310/3533
摘要： This invention discloses high-affinity oligonucleotide ligands to the thermostable Taq polymerase, Tth polymerase and TZ05 polymerase. Specifically, this invention discloses DNA ligands having the ability to bind to the Taq, Tth and TZ05 polymerases and the methods for obtaining such ligands. The ligands are capable of inhibiting polymerases at any predetermined temperature.
摘要翻译：本发明公开了耐热Taq聚合酶，Tth聚合酶和TZ05聚合酶的高亲和力寡核苷酸配体。具体地，本发明公开了具有结合Taq，Tth和TZ05聚合酶的能力的DNA配体和获得这些配体的方法。配体能够在任何预定温度下抑制聚合酶。

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