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1. 发明授权

EP3101144B1 SENSING APPARATUS AND METHOD 有权
公开(公告)号：EP3101144B1
公开(公告)日：2018-03-07
申请号：EP16179947.3
申请日：2013-07-18
申请人： DNAE GROUP HOLDINGS LIMITED
发明人： ANSARI, Zahid , AMIN, Krishna , JORGENSEN, Ginny , KOLB, Kurt , MORLEY, Daniel , PATEL, Alpesh , REED, Sam , SHEPHERD, Leila , TOUMAZOU, Christofer
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , B01L7/52 , B01L2200/147 , B01L2300/0627 , B01L2300/123 , B01L2300/1827 , C12Q1/6869 , C12Q2527/101 , C12Q2527/119 , C12Q2535/122 , C12Q2543/101 , C12Q2547/101 , C12Q2565/537 , C12Q2565/629
摘要： Provided is a sensing apparatus comprising a chip for integrated amplification and sequencing of a template polynucleotide in a sample. The apparatus comprises a chip with at least one ISFET in a well or chamber, amplification means for amplifying the template polynucleotide on a surface of said chip and comprising at least one heating means suitable for conducting amplification of the template polynucleotide at temperatures elevated with respect to room temperature, and sequencing means for sequencing the amplified template polynucleotide in said well or chamber. Methods of use are also provided.

2. 发明公开

EP3283656A1 METHODS FOR PERFORMING SPATIAL PROFILING OF BIOLOGICAL MOLECULES 审中-实审
标题翻译：实施生物分子空间分布的方法
公开(公告)号：EP3283656A1
公开(公告)日：2018-02-21
申请号：EP16781003.5
申请日：2016-04-18
申请人： Centrillion Technology Holdings Corporation
发明人： ZHOU, Wei , WARRINGTON, Janet
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12N15/1065 , C12N15/1068 , C12Q1/6804 , G01N33/54306 , G01N2458/10 , C12Q2535/122 , C12Q2543/101 , C12Q2565/514
摘要： Provided herein are methods and compositions for profiling the spatial distribution of a wide variety of biological molecules in a sample. The methods and compositions are suited for spatial labeling and sequencing of biological molecules (e.g., nucleic acids, proteins) in a biological sample.

3. 发明公开

EP3102698A1 PROXIMITY ASSAY WITH DETECTION BASED ON HYBRIDISATION CHAIN REACTION (HCR) 有权
标题翻译： PROXIMITÄTSAYAYMIT DETEKTION AUF GRUNDLAGE EINER HYBRIDISIERUNGSKETTENREAKTION（HCR）
公开(公告)号：EP3102698A1
公开(公告)日：2016-12-14
申请号：EP15702763.2
申请日：2015-02-04
申请人： Olink Bioscience AB
发明人： SÖDERBERG, Ola , KOOS, Björn , DUCANI, Cosimo , HÖGBERG, Björn
IPC分类号： C12Q1/68 , G01N33/53
CPC分类号： C12Q1/6818 , C07K16/00 , C12Q1/6804 , C12Q1/6841 , C12Q1/686 , G01N33/5308 , G01N33/54306 , G01N2458/10 , C12Q2525/186 , C12Q2525/301 , C12Q2531/113 , C12Q2565/1015 , C12Q2543/101
摘要： The present invention provides a method for detecting an analyte in a sample, said method comprising a) contacting said sample with a set of proximity probes comprising at least first and second proximity probes, which probes each comprise an analyte-binding domain capable of binding directly or indirectly to said analyte and a nucleic acid domain, such that the proximity probes can simultaneously bind, directly or indirectly, to the analyte, wherein i) the nucleic acid domains of said first and second proximity probes comprise regions capable of mediating an interaction involving said domains when under permissive conditions; and ii) the nucleic acid domain of one of said first and second probes comprises an HCR initiator region comprised within a metastable secondary structure such that it is unable to initiate an HCR reaction until released from said metastable secondary structure; b) introducing permissive conditions to allow the nucleic acid domains of said first and second probes to interact with each other when said probes have both bound directly or indirectly to the analyte, wherein said interaction results in unfolding of the metastable secondary structure of the nucleic acid domain of the first or second probe to release a single-stranded HCR initiator region; c) performing an HCR reaction using at least two HCR monomers, wherein the first HCR monomer comprises a region of complementarity to the HCR initiator region and hybridisation of the HCR initiator region to the first HCR polymer begins the HCR reaction to form a polymer; and d) detecting the polymer thereby to detect the analyte.
摘要翻译：本发明提供了一种用于检测样品中分析物的方法，所述方法包括：a）使所述样品与包含至少第一和第二接近探针的一组接近探针接触，所述探针各自包含能够直接结合的分析物结合结构域或间接地连接到所述分析物和核酸结构域，使得所述邻近探针可以同时直接或间接地结合到所述分析物，其中i）所述第一和第二邻近探针的核酸结构域包括能够介导相互作用的区域在许可条件下说域名; 和ii）所述第一和第二探针之一的核酸结构域包含包含在亚稳二级结构内的HCR引发区，使得其不能引发HCR反应，直到从所述亚稳二级结构释放; b）引入允许条件以允许所述第一和第二探针的核酸结构域彼此相互作用，当所述探针既直接或间接地与分析物结合时，其中所述相互作用导致核酸的亚稳二级结构的解折叠第一或第二探针的结构域以释放单链HCR引发区; c）使用至少两种HCR单体进行HCR反应，其中第一HCR单体包含与HCR引发剂区域互补的区域，并且HCR引发剂区域与第一HCR聚合物的杂交开始HCR反应以形成聚合物; 和d）检测聚合物从而检测分析物。

4. 发明授权

EP0868530B1 A CASCADE NUCLEIC ACID AMPLIFICATION REACTION 失效
标题翻译： ONE在DNA的CASCADE平整再现响应
公开(公告)号：EP0868530B1
公开(公告)日：2003-04-23
申请号：EP96939821.3
申请日：1996-12-05
申请人： Koch, Jorn, Erland
发明人： Koch, Jorn, Erland
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12Q1/6844 , C12N15/10 , C12Q1/6804 , C12Q1/682 , C12Q2525/301 , C12Q2531/125 , C12Q2543/101 , C12Q2525/143 , C12Q2563/131
摘要： A DNA template consisting of multiple tandem repetitions of an oligonucleotide unit is produced by stepwise copying of an oligonucleotide comprising at least one and a half units of a nucleotide sequence showing dyad symmetry by means of a template- and primer-dependent DNA polymerase in the presence of the necessary nucleoside triphosphates during repeated cycles of denaturation and annealing, or by endless copying of a circular oligonucleotide comprising at least one copy of said oligonucleotide unit by means of a nucleic acid polymerase, which is capable of strand displacement and is substantially without 5'-3' exonuclease activity, in the presence of the necessary nucleoside triphosphates and, if necessary, a primer capable of binding to some portion of the oligonucleotide, or by known reactions. Thereafter, in a cascade nucleic acid amplification reaction, a great number of partial and complete DNA or RNA copies of said DNA template is produced by means of a nucleic acid polymerase, which is capable of strand displacement and is substantially without 5'-3' exonuclease activity, by contacting the template with said nucleic acid polymerase in the presence of the necessary nucleoside triphosphates and, if necessary, a primer capable of binding to the oligonucleotide unit, the polymerase thus synthesizing DNA or RNA originating from, ideally, each repeating oligonucleotide unit in the DNA template. These reactions can be used in methods for detecting a target molecule or group and in processes for the amplification of a particular DNA sequence.

5. 发明公开

EP1300466A2 A cascade nucleic acid amplification reaction 失效
标题翻译：在Kaskaden的Eine在verlaufendeVervielfältigungsreaktionvonNukleinsäuren
公开(公告)号：EP1300466A2
公开(公告)日：2003-04-09
申请号：EP03000499.8
申请日：1996-12-05
申请人： Koch, Jorn, Erland
发明人： Koch, Jorn, Erland
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12Q1/6844 , C12N15/10 , C12Q1/6804 , C12Q1/682 , C12Q2525/301 , C12Q2531/125 , C12Q2543/101 , C12Q2525/143 , C12Q2563/131
摘要： A DNA template consisting of multiple tandem repetitions of an oligonucleotide unit is produced by endless copying of a circular oligonucleotide comprising at least one copy of said oligonucleotide unit by means of a nucleic acid polymerase, which is capable of strand displacement and is substantially without 5'-3' exonuclease activity, in the presence of the necessary nucleoside triphosphates and, if necessary, a primer capable of binding to some portion of the oligonucleotide. This reaction can be used in a process for the amplification of a particular DNA sequence wherein said DNA sequence is either circularized or inserted into a circular oligonucleotide, and the resulting circular DNA is used as a template for an endless copying process.
摘要翻译：由寡核苷酸单元的多重串联重复组成的DNA模板是通过无限复制的包含至少一个拷贝的所述寡核苷酸单元的环状寡核苷酸产生的，该核酸聚合酶能够进行链排列并基本上不含5' -3'核酸外切酶活性，在必需的核苷三磷酸存在下，如果需要，可以与能够与寡核苷酸的一部分结合的引物。该反应可用于扩增特定DNA序列的方法，其中所述DNA序列被环化或插入到环状寡核苷酸中，所得到的环状DNA用作环状复制过程的模板。

6. 发明授权

EP0863213B1 Thermal cycler equipment 失效
标题翻译：热循环仪
公开(公告)号：EP0863213B1
公开(公告)日：2001-10-31
申请号：EP98200769.2
申请日：1992-07-22
申请人： PE Corporation (NY) , THE RESEARCH FOUNDATION OF STATE UNIVERSITY OF NEW YORK
发明人： Bloch, Will , Nuovo, Gerard J.
IPC分类号： C12Q1/68 , C12M1/38 , B01L3/00 , B01L7/00
CPC分类号： C12Q1/6841 , B01L7/52 , C12Q1/686 , C12Q2549/101 , C12Q2543/101 , C12Q2522/101

7. 发明公开

EP3384048A1 METHODS AND COMPOSITIONS FOR NUCLEIC ACID ANALYSIS 有权转让
公开(公告)号：EP3384048A1
公开(公告)日：2018-10-10
申请号：EP16822294.1
申请日：2016-12-02
申请人： 10X Genomics, Inc.
发明人： ZHENG, Xinying , SAXONOV, Serge , SCHNALL-LEVIN, Michael , NESS, Kevin , BHARADWAJ, Rajiv
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/6806 , C12Q1/6834 , C12Q1/6869 , C12Q2521/501 , C12Q2523/101 , C12Q2525/155 , C12Q2525/191 , C12Q2535/122 , C12Q2543/101 , C12Q2563/149 , C12Q2563/159 , C12Q2563/179 , C12Q2565/631
摘要： The present invention is directed to methods, compositions and systems for analyzing sequence information while retaining structural and molecular context of that sequence information.

8. 发明公开

EP3167044A1 MICROFLUIDIC DEVICE 有权转让
公开(公告)号：EP3167044A1
公开(公告)日：2017-05-17
申请号：EP15818165.1
申请日：2015-06-12
申请人： Elf, Johan
发明人： BALTEKIN, Özden , ANDERSSON, Dan I.
IPC分类号： C12M1/34 , B01L3/00 , C12N1/04
CPC分类号： C12Q1/18 , B01L3/502761 , C12N15/1093 , C12Q1/6806 , C12Q1/6841 , C12Q1/6874 , G01N33/4833 , C12Q2563/185 , C12Q2521/501 , C12Q2525/185 , C12Q2531/125 , C12Q2543/101 , C12Q2565/601 , C12Q2565/629
摘要： A microfluidic device comprises a substrate transparent for imaging and having a plurality of spatially defined and separated cell channels having a dimension to accommodate cells in monolayer. A respective first end of the cell channels is in fluid connection with a flow input channel having a first end in fluid connection with a first fluid port and a second end in fluid connection with a second fluid port. A respective second end of the cell channels is in fluid connection with a first end of a respective wash channel having a second end in fluid connection with a flow output channel. The flow output channel is in fluid connection with a third fluid port. The wash channels have a dimension too small to accommodate the cells.
摘要翻译：微流体装置（1）包括透明用于成像的基底（10），并具有多个空间限定和分离的细胞通道（20），其具有适应单层细胞的尺寸。细胞通道（20）的相应的第一端（22）与具有与第一流体端口（31）流体连接的第一端（32）和第二端（34）的流输入通道（30）流体连接。）与第二流体端口（33）流体连接。细胞通道（20）的相应第二端（24）与相应洗涤通道（40）的第一端（42）流体连接，该洗涤通道具有与流动输出通道（50）流体连接的第二端（44））。流动输出通道（50）与第三流体端口（51）流体连接。清洗通道（40）的尺寸太小而不能适应细胞。

9. 发明公开

EP2029745A1 DIAGNOSTIC METHODS AND MARKERS 有权
标题翻译：诊断过程后标记
公开(公告)号：EP2029745A1
公开(公告)日：2009-03-04
申请号：EP07808640.2
申请日：2007-06-07
申请人： Otago Innovation Limited
发明人： ASSINDER, Stephen, John , STANTON, Jo-Ann
IPC分类号： C12N15/11 , A61B5/00 , C12Q1/00
CPC分类号： C07K14/4748 , C12Q1/6816 , C12Q1/6886 , G01N33/5023 , G01N33/57434 , G01N2500/00 , G01N2800/52 , C12Q2543/101
摘要： The present invention relates to methods of detecting, monitoring and treating prostate cancer (PRC) OR prostatic intraepithelial neoplasia (PIN) or a predisposition to same. Provided for use in the methods is a novel cancer marker, PSPU43, as well as bioassays and kits.

10. 发明公开

EP1007728A4 NUCLEIC ACID AMPLIFICATION METHOD: HYBRIDIZATION SIGNAL AMPLIFICATION METHOD (HSAM) 失效
标题翻译：方法的核酸扩增：METHOD FOR杂交信号GAIN
公开(公告)号：EP1007728A4
公开(公告)日：2004-04-07
申请号：EP97935207
申请日：1997-07-30
申请人： ZHANG DAVID Y , BRANDWEIN MARGARET
发明人： ZHANG DAVID Y , BRANDWEIN MARGARET
IPC分类号： C12N15/09 , C12Q1/68 , G01N33/53
CPC分类号： C12Q1/6844 , C12Q1/6813 , C12Q1/682 , C12Q1/6834 , C12Q1/686 , C12Q1/6862 , C12Q1/6865 , C12Q2563/131 , C12Q2531/125 , C12Q2531/113 , C12Q2543/101
摘要： An improved method allowing for rapid sensitive and standardized detection of a target nucleic acid from a pathogenic microorganism or virus or normal or abnormal gene in a sample is provided. The method involves hybridizing a target nucleic acid to several non-overlapping oligonucleotide probes that hybridize to adjacent regions in the target nucleic acid, the probes being referred to capture/amplification probes and amplification probes, respectively, in the presence of paramagnetic beads coated with a ligand binding moiety. Through the binding of a ligand attached to one end of the capture/amplification probe and the specific hybridization of portions of the probes to adjacent sequences in the target nucleic acid, a complex comprising the target nucleic acid, the probes and the paramagnetic beads is formed. The probes may then be ligated together to form a contiguous ligated amplification sequence bound to the beads, which complex may be denatured to remove the target nucleic acid and unligated probes. Alternatively, separate capture and amplification probes may be used which form continuous full-length or circular probes, and may be directly detected or amplified using a suitable amplification technique, e.g., PCR, RAM or HSAM for detection. The detection of the ligated amplification sequence, either directly or following amplification of the ligated amplification sequence, indicates the presence of the target nucleic acid in a sample. Methods for the detection of the ligated amplification sequence, including hybridization signal amplification method and ramification-extension amplification method, are also provided.

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