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1. 发明授权

EP0868530B1 A CASCADE NUCLEIC ACID AMPLIFICATION REACTION 失效
标题翻译： ONE在DNA的CASCADE平整再现响应
公开(公告)号：EP0868530B1
公开(公告)日：2003-04-23
申请号：EP96939821.3
申请日：1996-12-05
申请人： Koch, Jorn, Erland
发明人： Koch, Jorn, Erland
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12Q1/6844 , C12N15/10 , C12Q1/6804 , C12Q1/682 , C12Q2525/301 , C12Q2531/125 , C12Q2543/101 , C12Q2525/143 , C12Q2563/131
摘要： A DNA template consisting of multiple tandem repetitions of an oligonucleotide unit is produced by stepwise copying of an oligonucleotide comprising at least one and a half units of a nucleotide sequence showing dyad symmetry by means of a template- and primer-dependent DNA polymerase in the presence of the necessary nucleoside triphosphates during repeated cycles of denaturation and annealing, or by endless copying of a circular oligonucleotide comprising at least one copy of said oligonucleotide unit by means of a nucleic acid polymerase, which is capable of strand displacement and is substantially without 5'-3' exonuclease activity, in the presence of the necessary nucleoside triphosphates and, if necessary, a primer capable of binding to some portion of the oligonucleotide, or by known reactions. Thereafter, in a cascade nucleic acid amplification reaction, a great number of partial and complete DNA or RNA copies of said DNA template is produced by means of a nucleic acid polymerase, which is capable of strand displacement and is substantially without 5'-3' exonuclease activity, by contacting the template with said nucleic acid polymerase in the presence of the necessary nucleoside triphosphates and, if necessary, a primer capable of binding to the oligonucleotide unit, the polymerase thus synthesizing DNA or RNA originating from, ideally, each repeating oligonucleotide unit in the DNA template. These reactions can be used in methods for detecting a target molecule or group and in processes for the amplification of a particular DNA sequence.

2. 发明公开

EP1300466A2 A cascade nucleic acid amplification reaction 失效
标题翻译：在Kaskaden的Eine在verlaufendeVervielfältigungsreaktionvonNukleinsäuren
公开(公告)号：EP1300466A2
公开(公告)日：2003-04-09
申请号：EP03000499.8
申请日：1996-12-05
申请人： Koch, Jorn, Erland
发明人： Koch, Jorn, Erland
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12Q1/6844 , C12N15/10 , C12Q1/6804 , C12Q1/682 , C12Q2525/301 , C12Q2531/125 , C12Q2543/101 , C12Q2525/143 , C12Q2563/131
摘要： A DNA template consisting of multiple tandem repetitions of an oligonucleotide unit is produced by endless copying of a circular oligonucleotide comprising at least one copy of said oligonucleotide unit by means of a nucleic acid polymerase, which is capable of strand displacement and is substantially without 5'-3' exonuclease activity, in the presence of the necessary nucleoside triphosphates and, if necessary, a primer capable of binding to some portion of the oligonucleotide. This reaction can be used in a process for the amplification of a particular DNA sequence wherein said DNA sequence is either circularized or inserted into a circular oligonucleotide, and the resulting circular DNA is used as a template for an endless copying process.
摘要翻译：由寡核苷酸单元的多重串联重复组成的DNA模板是通过无限复制的包含至少一个拷贝的所述寡核苷酸单元的环状寡核苷酸产生的，该核酸聚合酶能够进行链排列并基本上不含5' -3'核酸外切酶活性，在必需的核苷三磷酸存在下，如果需要，可以与能够与寡核苷酸的一部分结合的引物。该反应可用于扩增特定DNA序列的方法，其中所述DNA序列被环化或插入到环状寡核苷酸中，所得到的环状DNA用作环状复制过程的模板。

3. 发明授权

EP1300466B1 A cascade nucleic acid amplification reaction 失效
标题翻译：延伸在核酸扩增级联反应
公开(公告)号：EP1300466B1
公开(公告)日：2006-04-26
申请号：EP03000499.8
申请日：1996-12-05
申请人： Koch, Jorn, Erland
发明人： Koch, Jorn, Erland
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12Q1/6844 , C12N15/10 , C12Q1/6804 , C12Q1/682 , C12Q2525/301 , C12Q2531/125 , C12Q2543/101 , C12Q2525/143 , C12Q2563/131

4. 发明公开

EP0868530A1 A CASCADE NUCLEIC ACID AMPLIFICATION REACTION 失效
标题翻译：在KASKADEN VELAUFENDEVERVIELFÄLTIGUNGSREAKTIONVONNUKLEINSÄUREN
公开(公告)号：EP0868530A1
公开(公告)日：1998-10-07
申请号：EP96939821.0
申请日：1996-12-05
申请人： Koch, Jorn, Erland
发明人： Koch, Jorn, Erland
IPC分类号： C12N15 , C12Q1
CPC分类号： C12Q1/6844 , C12N15/10 , C12Q1/6804 , C12Q1/682 , C12Q2525/301 , C12Q2531/125 , C12Q2543/101 , C12Q2525/143 , C12Q2563/131
摘要： A DNA template consisting of multiple tandem repetitions of an oligonucleotide unit is produced by endless copying of a circular oligonucleotide comprising at least one copy of said oligonucleotide unit by means of a nucleic acid polymerase, which is capable of strand displacement and is substantially without 5'-3' exonuclease activity, in the presence of the necessary nucleoside triphosphates and, if necessary, a primer capable of binding to some portion of the oligonucleotide. This reaction can be used in a process for the amplification of a particular DNA sequence wherein said DNA sequence is either circularized or inserted into a circular oligonucleotide, and the resulting circular DNA is used as a template for an endless copying process.
摘要翻译：由寡核苷酸单元的多重串联重复组成的DNA模板是通过无限复制的包含至少一个拷贝的所述寡核苷酸单元的环状寡核苷酸产生的，该核酸聚合酶能够进行链排列并基本上不含5' -3'核酸外切酶活性，在必需的核苷三磷酸存在下，如果需要，可以与能够与寡核苷酸的一部分结合的引物。该反应可用于扩增特定DNA序列的方法，其中所述DNA序列被环化或插入到环状寡核苷酸中，所得到的环状DNA用作环状复制过程的模板。

5. 发明公开

EP1300466A3 A cascade nucleic acid amplification reaction 失效
标题翻译：延伸在核酸扩增级联反应
公开(公告)号：EP1300466A3
公开(公告)日：2004-01-02
申请号：EP03000499.8
申请日：1996-12-05
申请人： Koch, Jorn, Erland
发明人： Koch, Jorn, Erland
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12Q1/6844 , C12N15/10 , C12Q1/6804 , C12Q1/682 , C12Q2525/301 , C12Q2531/125 , C12Q2543/101 , C12Q2525/143 , C12Q2563/131
摘要： A DNA template consisting of multiple tandem repetitions of an oligonucleotide unit is produced by endless copying of a circular oligonucleotide comprising at least one copy of said oligonucleotide unit by means of a nucleic acid polymerase, which is capable of strand displacement and is substantially without 5'-3' exonuclease activity, in the presence of the necessary nucleoside triphosphates and, if necessary, a primer capable of binding to some portion of the oligonucleotide. This reaction can be used in a process for the amplification of a particular DNA sequence wherein said DNA sequence is either circularized or inserted into a circular oligonucleotide, and the resulting circular DNA is used as a template for an endless copying process.

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