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1. 发明授权

EP3360972B1 SINGLE MOLECULE NUCLEIC ACID SEQUENCE ANALYSIS PROCESSES 有权
公开(公告)号：EP3360972B1
公开(公告)日：2019-12-11
申请号：EP18158658.7
申请日：2009-01-15
申请人： Sequenom, Inc.
发明人： CANTOR, Charles R.
IPC分类号： C12Q1/6806

2. 发明公开

EP3360972A1 SINGLE MOLECULE NUCLEIC ACID SEQUENCE ANALYSIS PROCESSES AND COMPOSITIONS 有权
公开(公告)号：EP3360972A1
公开(公告)日：2018-08-15
申请号：EP18158658.7
申请日：2009-01-15
申请人： Sequenom, Inc.
发明人： CANTOR, Charles R.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6806 , C12Q1/6837 , C12Q1/6844 , C12Q2565/537 , C12Q2565/519 , C12Q2565/501 , C12Q2531/101 , C12Q2525/161 , C12Q2525/155
摘要： The present invention relates to a method for preparing sample nucleic acid complements, which comprises: a. preparing a mixture comprising sample nucleic acid and a collection of solid supports under conditions in which a single molecule of the sample nucleic acid hybridizes to a nucleic acid molecule on a solid support in the collection, wherein: the nucleic acid molecules on each solid support in the collection comprise a single-stranded solid phase nucleic acid species including a primer sequence, an identifier sequence and a probe sequence orientated 5' - (primer sequence) - (identifier sequence) - (probe sequence) - 3'; the probe sequence of each solid phase nucleic acid species on a solid support in the collection hybridizes to a subsequence of the sample nucleic acid sequence when the probe sequence is complementary to a nucleotide subsequence in the sample nucleic acid sequence; the solid phase nucleic acid species of each solid support in the collection share a common probe sequence or the solid phase nucleic acid species do not share a common probe sequence with other solid phase nucleic acid species on the solid support; the solid phase nucleic acid species on each solid support in the collection share a common identifier sequence; and the sample nucleic acid is single-stranded nucleic acid; and b. contacting the mixture with extension reagents under conditions in which solid phase nucleic acid species hybridized to sample nucleic acid is extended by the reagents; whereby sample nucleic acid complements comprising the shared, unique identifier sequence of a solid support in the collection and comprising subsequences of the sample nucleic acid sequence are prepared.

3. 发明公开

EP3699291A1 SINGLE MOLECULE NUCLEIC ACID SEQUENCE ANALYSIS PROCESSES AND COMPOSITIONS 有权
公开(公告)号：EP3699291A1
公开(公告)日：2020-08-26
申请号：EP19214834.4
申请日：2009-01-15
申请人： Sequenom, Inc.
发明人： CANTOR, Charles R.
IPC分类号： C12Q1/68 , C12Q1/6806 , C12Q1/6837 , C12Q1/6844
摘要： Improved solid supports and methods for analyzing target nucleotide sequences are provided herein. Certain improvements are directed to efficiently preparing nucleic acids that comprise nucleotide sequences identical to or substantially identical to one or more target nucleotide sequences, or complement thereof. The prepared nucleic acids include a reference sequence that facilitates sequence analysis. The solid supports and methods provided herein minimize the number of steps required by published sequence analysis methodologies, and thereby offer improved sequence analysis efficiency.

4. 发明公开

EP3584324A1 NUCLEIC ACID QUANTIFICATION PRODUCTS AND PROCESSES 审中-公开
公开(公告)号：EP3584324A1
公开(公告)日：2019-12-25
申请号：EP19169246.6
申请日：2009-11-20
申请人： Sequenom, Inc.
发明人： CANTOR, Charles R.
IPC分类号： C12Q1/68 , C12N15/10 , C12Q1/6837
摘要： Described herein are products and processes for nucleic acid quantification, which are in part useful for detecting and determining the nucleotide sequence of rare nucleic acids (i.e., low copy number nucleic acids) in a sample. Such products and processes are useful for reducing the dynamic range among different nucleic acid species.

5. 发明公开

EP4450642A2 SINGLE MOLECULE NUCLEIC ACID SEQUENCE ANALYSIS PROCESSES AND COMPOSITIONS 审中-公开
公开(公告)号：EP4450642A2
公开(公告)日：2024-10-23
申请号：EP24192893.6
申请日：2009-01-15
申请人： Sequenom, Inc.
发明人： CANTOR, Charles R.
IPC分类号： C12Q1/6844
CPC分类号： C12Q1/6806 , C12Q1/6844 , C12Q1/6837
摘要： The present invention relates to a collection of solid supports comprising single-stranded nucleic acid species, (a) wherein each solid support in the collection comprises more than one single-stranded nucleic acid species covalently linked to the solid support, wherein each single-stranded nucleic acid species linked to a common solid support comprises an identifier sequence and a probe sequence, wherein the single-stranded nucleic acid species of each solid support in the collection further comprises a primer sequence, and (b) wherein all the single-stranded nucleic acid species linked to the common solid support share a common identifier sequence, and wherein the identifier sequence from the more than one single-stranded nucleic acid species linked to the common solid support in the collection is different from the identifier sequences of the single-stranded nucleic acid species linked to the other solid supports in the collection, whereby each solid support in the collection is associated with a unique identifier sequence each differing from all other identifier sequences by one or more nucleotides, when the identifier sequences are aligned with one another; and (c) wherein the probe sequence of each of the more than one single-stranded nucleic acid species linked to the solid support allows the more than one single-stranded nucleic acid to hybridize to a subsequence in a sample nucleic acid, and (d) wherein the probe sequences of the more than one single-stranded nucleic acid species on each solid support comprise a plurality of different probe sequence species, wherein each probe sequence species differs from all other probe sequence species by one or more nucleotides, when the probe sequence species are aligned with one another.

6. 发明授权

EP3181702B1 IMPROVING NUCLEIC ACID QUANTITATION BY COMPRESSING THE DYNAMIC RANGE OF HIGH-ABUNDANCE MOLECULES 有权
公开(公告)号：EP3181702B1
公开(公告)日：2019-04-17
申请号：EP16194196.8
申请日：2009-11-20
申请人： Sequenom, Inc.
发明人： CANTOR, Charles R.
IPC分类号： C12Q1/6837

7. 发明公开

EP3181702A1 NUCLEIC ACID QUANTIFICATION PRODUCTS AND PROCESSES 审中-公开
标题翻译：核酸量化产品和工艺
公开(公告)号：EP3181702A1
公开(公告)日：2017-06-21
申请号：EP16194196.8
申请日：2009-11-20
申请人： Sequenom, Inc.
发明人： CANTOR, Charles R.
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12Q1/6837 , C12Q2537/159 , C12Q2565/518 , C12Q2545/114 , C12Q2525/161
摘要： Described herein are products and processes for nucleic acid quantification, which are in part useful for detecting and determining the nucleotide sequence of rare nucleic acids (i.e., low copy number nucleic acids) in a sample. Such products and processes are useful for reducing the dynamic range among different nucleic acid species.
摘要翻译：本文描述了用于核酸定量的产物和方法，其部分用于检测和确定样品中稀有核酸（即低拷贝数核酸）的核苷酸序列。这样的产品和方法可用于降低不同核酸种类之间的动态范围。

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