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1. 发明授权

EP3161152B1 METHODS AND COMPOSITIONS USING ONE-SIDED TRANSPOSITION 有权
公开(公告)号：EP3161152B1
公开(公告)日：2018-12-26
申请号：EP15734543.0
申请日：2015-06-26
申请人： Illumina, Inc.
发明人： STEEMERS, Frank J. , FISHER, Jeffrey S. , GUNDERSON, Kevin L. , AMINI, Sasan , GLOECKNER, Christian
IPC分类号： C12Q1/68 , C12N9/10 , C12N15/10
CPC分类号： C12N15/1093 , C12N9/22 , C12Q1/6806 , C12Q2521/507 , C12Q2565/514 , C12Q2521/101 , C12Q2535/122 , C12Q2563/179
摘要： Embodiments provided herein relate to methods and compositions for next generation sequencing. Some embodiments include the preparation of a template library from a target nucleic acid using one-sided transposition, and sequencing the template library.

2. 发明公开

EP3368668A1 METHOD AND APPARATUS FOR ENCODING CELLULAR SPATIAL POSITION INFORMATION 有权
公开(公告)号：EP3368668A1
公开(公告)日：2018-09-05
申请号：EP16860845.3
申请日：2016-10-27
申请人： Silicon Valley Scientific, Inc.
发明人： JOVANOVICH, Stevan, B. , WAGNER, Peter
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12N15/1003 , C12N15/1017 , C12N15/1093 , C12Q1/6806 , C12Q2525/191 , C12Q2535/122 , C12Q2563/179 , C12Q2525/161 , C12Q2525/173 , C12Q2563/149 , C12Q2563/159 , C12Q2565/514 , C12Q2521/507
摘要： A system, methods, and apparatus are described to collect and prepare single cells and groups of cells from microsamples of specimens and encode spatial information of the physical position of the cells in the specimen. In some embodiment, beads or surfaces with oligonucleotides containing spatial barcodes are used to analyze DNA or RNA. The spatial barcodes allow the position of the cell to be defined and the nucleic acid sequencing information, such as target sequencing, whole genome, gene expression, used to analyze the cells in a microsample for cell type, expression pattern, DNA sequence, and other information, in the context of the cell's physical position in the specimen. In other embodiment, markers such as isotopes are added to a microsample to encode spatial position with mass spectoscopy or other analysis. The spatial encoded information is then readout by analysis such as DNA sequencing, mass spectrometry, fluorescence, or other methods.

3. 发明授权

EP2694666B1 MONITORING RECOMBINASE POLYMERASE AMPLIFICATION MIXTURES 有权转让
公开(公告)号：EP2694666B1
公开(公告)日：2018-05-23
申请号：EP12767452.1
申请日：2012-04-06
申请人： Alere San Diego, Inc.
发明人： ARMES, Niall A. , PIEPENBURG, Olaf , GREENWOOD, Catherine Jean
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6851 , C12Q1/6844 , C12Q1/689 , C12Q2600/158 , G01N21/6428 , G01N21/6456 , G01N2021/6432 , Y02A50/53 , C12Q2521/507 , C12Q2522/101 , C12Q2527/137 , C12Q2565/629 , C12Q2563/155 , C12Q2563/159
摘要： A process includes providing a mixture that includes a recombinase, a single-strand binding protein, and one or more oligonucleotides; and detecting particles in the reaction mixture.

4. 发明公开

EP3290518A1 NOVEL GENE TARGETING METHOD 审中-公开
标题翻译：新型基因靶向方法
公开(公告)号：EP3290518A1
公开(公告)日：2018-03-07
申请号：EP16785983.4
申请日：2016-04-29
申请人： Hangzhou Genekine Biotech Co., Ltd
发明人： JIANG, Youwei , LI, Yunfei
IPC分类号： C12N15/11 , C12N1/16 , C12P21/00
CPC分类号： C12N15/905 , C12N1/16 , C12N9/1241 , C12N15/11 , C12N15/902 , C12N2800/30 , C12P21/00 , C12N15/102 , C12Q2521/507
摘要： Provided are a novel two-step gene targeting method and a nucleotide construct for gene targeting. The method can improve the gene targeting efficiency and accurately identify a target gene knock-out mutant. The method of the present invention comprises: firstly, efficiently replacing a target gene in a genome with a targeting box by homologous recombination, the targeting box consisting of a target gene activity variant, a marker gene and site-specific recombination sites; and secondly, resecting the targeting box by recombinase, leaving a site-specific recombination site on the target gene to generate a target gene knock-out mutant, and removing a recombinase expression vector from the knock-out mutant by using a counter selection marker in the recombinase expression vector.
摘要翻译：提供了一种新的两步基因靶向方法和用于基因靶向的核苷酸构建体。该方法可以提高基因靶向效率并准确鉴定靶基因敲除突变体。本发明的方法包括：首先通过同源重组，有效置换基因组中的靶基因，所述靶向盒由靶基因活性变异体，标记基因和位点特异性重组位点组成; 其次，通过重组酶切除靶向盒，在靶基因上留下位点特异性重组位点以产生靶基因敲除突变体，并通过使用中的计数选择标记从敲除突变体中去除重组酶表达载体重组酶表达载体。

5. 发明公开

EP3227461A1 HIGH-THROUGHPUT SEQUENCING OF POLYNUCLEOTIDES 无效
标题翻译：多核苷酸的高通量测序
公开(公告)号：EP3227461A1
公开(公告)日：2017-10-11
申请号：EP15819931.5
申请日：2015-12-04
申请人： Amyris, Inc.
发明人： DEAN, Erik Jedediah , HOLMES, Victor , REEVES, Christopher , SHAPLAND, Elaine
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12Q1/6806 , C12N15/1003 , C12N15/1093 , C12N15/66 , C12Q2521/507 , C12Q2525/191 , C12Q2535/122 , C12Q2563/179
摘要： Provided herein are methods, compositions, and kits for simultaneously sequencing polynucleotides from a plurality of samples in a single sequencing run. In an embodiment, the present invention improves efficiency of the next-generation sequencing process, in part, by reducing reaction volumes to a sub-microliter range and generating and using a set of novel barcode sequences to tag a plurality of polynucleotides. In addition, the sample preparation processes have been simplified to save time and cost, while providing high-quality sequence coverage for all samples.
摘要翻译：本文提供了用于在单次测序运行中同时测序来自多个样品的多核苷酸的方法，组合物和试剂盒。在一个实施方案中，本发明部分地通过将反应体积减少至亚微升范围并产生并使用一组新的条形码序列来标记多个多核苷酸来提高下一代测序过程的效率。此外，样品制备过程已经过简化，以节省时间和成本，同时为所有样品提供高质量的序列覆盖率。

6. 发明授权

EP2895620B1 NUCLEIC ACID AMPLIFICATION 有权转让
公开(公告)号：EP2895620B1
公开(公告)日：2017-08-02
申请号：EP13771260.0
申请日：2013-09-10
申请人： Life Technologies Corporation
发明人： LI, Chieh-Yuan , RUFF, David , CHEN, Shiaw-Min , O'NEIL, Jennifer , KASINSKAS, Rachel , ROTHBERG, Jonathan , HINZ, Wolfgang
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6846 , C12Q2521/101 , C12Q2521/507 , C12Q2522/101 , C12Q2527/101

7. 发明公开

EP3143157A1 METHODS OF IDENTIFYING ENZYMES AND MICROORGANISMS 审中-公开
标题翻译：密克罗尼西亚VERFAHREN ZUR IDENTIFIZIERUNG VON ENZYMEN
公开(公告)号：EP3143157A1
公开(公告)日：2017-03-22
申请号：EP15723908.8
申请日：2015-05-15
申请人： Zymonostics ApS
发明人： STOUGAARD, Magnus , KNUDSEN, Birgitta Ruth , HEDE, Marianne Smedegaard , THOMSEN, Jonas , HO, Yi-Ping
IPC分类号： C12Q1/68 , C12Q1/02 , C12Q1/04 , G01N33/573 , C12Q1/25 , C12Q1/533
CPC分类号： C12Q1/6893 , C12Q1/025 , C12Q1/04 , C12Q1/25 , C12Q1/485 , C12Q1/533 , C12Q1/68 , C12Q1/689 , G01N33/573 , G01N2333/9015 , G01N2333/91245 , G01N2800/26 , Y02A50/58 , C12Q2521/501 , C12Q2533/107 , C12Q2565/629 , C12Q2521/507 , C12Q2521/519
摘要： The invention provides a method of identifying a microorganism that expresses a nucleic acid-modifying enzyme, in sample, the method comprising: (a) contacting a nucleic acid substrate targeted by the nucleic acid-modifying enzyme with the sample; (b) adding a further nucleic acid molecule to the sample which nucleic acid molecule is ligated to the nucleic acid substrate in the presence of the nucleic acid-modifying enzyme to form a ligation product which comprises a linear single strand of nucleic acid that is capable of being detected; and (c) detecting the presence of the ligation product.
摘要翻译：本发明提供了一种鉴定在样品中表达核酸修饰酶的微生物的方法，所述方法包括：（a）使核酸修饰酶靶向的核酸底物与样品接触; （b）在所述样品中加入另外的核酸分子，所述核酸分子在所述核酸修饰酶存在下与所述核酸底物连接以形成连接产物，所述连接产物包含能够具有的线性单链核酸被检测到和（c）检测结扎产物的存在。

8. 发明授权

EP2612925B1 KIT FOR THE PRODUCTION OF CLOSED LINEAR DNA 有权转让
标题翻译： KIT用于生产闭合线性DNA
公开(公告)号：EP2612925B1
公开(公告)日：2016-11-30
申请号：EP13152077.7
申请日：2010-02-01
申请人： Touchlight IP Limited
发明人： HILL, Vanessa
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , A61K39/00 , A61K48/00 , A61K2039/53 , C12N9/0069 , C12N15/11 , C12Q1/6844 , C12Q1/6846 , C12Y113/12007 , C12Q2521/113 , C12Q2521/507 , C12Q2531/125 , C12Q2525/301
摘要： An in vitro process for the production of closed linear deoxyribonucleic acid (DNA) comprises (a) contacting a DNA template comprising at least one protelomerase target sequence with at least one DNA polymerase in the presence of one or more primers under conditions promoting amplification of said template; and (b) contacting amplified DNA produced in (a) with at least one protelomerase under conditions promoting production of closed linear DNA. A kit provides components necessary in the process.

9. 发明公开

EP3095879A1 NUCLEIC ACID AMPLIFICATION 有权
标题翻译：核酸扩增
公开(公告)号：EP3095879A1
公开(公告)日：2016-11-23
申请号：EP16177421.1
申请日：2013-03-15
申请人： Life Technologies Corporation
发明人： LI, Chieh-Yuan , RUFF, David , CHEN, Shiaw-Min , O'NEIL, Jennifer , KASINSKAS, Rachel , ROTHBERG, Jonathan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q2521/507 , C12Q2527/101 , C12Q2527/153 , C12Q2535/122 , C12Q2563/155 , C12Q2563/159 , C12Q2565/537 , C12Q2565/607
摘要： In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming an reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of an reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.
摘要翻译：在一些实施方案中，本教导提供了用于核酸扩增的方法，包括形成反应混合物，并使反应混合物经受适于核酸扩增的条件。在一些实施方案中，用于核酸扩增的方法包括使待扩增的核酸经历部分变性条件。在一些实施方案中，用于核酸扩增的方法包括扩增而不完全变性扩增的核酸。在一些实施方案中，用于核酸扩增的方法使用催化同源重组和聚合酶的酶。在一些实施方案中，用于核酸扩增的方法可以在单个反应容器中进行。在一些实施方案中，用于核酸扩增的方法可以在反应混合物的单一连续液相中进行，而不需要反应混合物的区室化或反应组分的固定化。在一些实施方案中，用于核酸扩增的方法包括在等温扩增条件下任选地在聚合物存在下将至少一种多核苷酸扩增到表面上。聚合物可以包含筛分剂和/或扩散减少剂。

10. 发明公开

EP3088533A1 RECOMBINASE POLYMERASE AMPLIFICATION 有权转让
标题翻译： REKOMBINASE - 聚合酶AMPLIFIKATION
公开(公告)号：EP3088533A1
公开(公告)日：2016-11-02
申请号：EP16157807.5
申请日：2007-05-04
申请人： Alere San Diego, Inc.
发明人： Armes, Niall Antony , Piepenburg, Olaf , Parker, Mathew James David
IPC分类号： C12Q1/68
CPC分类号： C12N9/1241 , C12N9/00 , C12Q1/6844 , C12Y207/07 , C12Q2521/507 , C12Q2531/119
摘要： The present invention features novel, diverse, hybrid and engineered recombinase enzymes, and the utility of such proteins with associated recombination factors for carrying out DNA amplification assays. The present invention also features different recombinase 'systems' having distinct biochemical activities in DNA amplification assays, and differing requirements for loading factors, single-stranded DNA binding proteins (SSBs), and the quantity of crowding agent employed.
摘要翻译：本发明的特征在于新颖，多样的，杂交和工程重组酶，以及具有相关重组因子的这种蛋白质用于进行DNA扩增测定。本发明还具有在DNA扩增测定中具有不同生物化学活性的不同重组酶“系统”，以及对负载因子，单链DNA结合蛋白（SSB）的不同要求以及所用的拥挤剂的量。

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