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1. 发明公开

EP3303621A1 SURFACE-BASED TAGMENTATION 审中-实审转让
标题翻译：基于表面的编码
公开(公告)号：EP3303621A1
公开(公告)日：2018-04-11
申请号：EP16728718.4
申请日：2016-05-27
申请人： Illumina Cambridge Limited
发明人： GORMLEY, Niall Anthony , IOANNOU, Avgousta , JACKSON, Rosamond , MORRELL, Natalie
IPC分类号： C12Q1/68 , C12N15/10 , C12N9/22
CPC分类号： C12N15/1065 , B01J2219/00351 , B01J2219/00596 , B01J2219/00641 , C12N9/22 , C12N15/1017 , C12Q1/6806 , C12Q1/686 , C12Q2521/543 , C12Q2525/191 , C12Q2563/179 , C40B50/14 , C12Q2535/122 , C12Q2565/518
摘要： Presented herein are methods and compositions surface-based tagmentation. In particular embodiments, methods of preparing an immobilized library of fragmented and tagged DNA molecules on a solid surface are presented. In particular embodiments, the solid surface comprises immobilized transposomes in a dried format, suitable for reconstitution upon contact with liquid, such as a liquid sample.

2. 发明授权

EP2689028B1 ISOLATION OF POLYMERASE-NUCLEIC ACID COMPLEXES AND LOADING ONTO SUBSTRATES 有权转让
标题翻译：聚合酶 - 核酸复合物的分离和加载到基质上
公开(公告)号：EP2689028B1
公开(公告)日：2017-08-30
申请号：EP12761363.6
申请日：2012-03-20
申请人： Pacific Biosciences Of California, Inc.
发明人： THANG, Pham , GRAY, Jeremy , FEHR, Adrian , BHAMIDIPATI, Arunashree , CHMURA, Aj , TRAVERS, Kevin , OLIVARES, Eric , TSAI, Yu-Chih
IPC分类号： C12Q1/48 , C12Q1/68 , C12N15/11 , G01N27/26 , G01N33/68
CPC分类号： C12Q1/6874 , B01L9/523 , C12Q1/6869 , C40B60/14 , G01N33/54313 , G01N33/54353 , G01N2333/922 , C12Q2521/543 , C12Q2523/303 , C12Q2525/301

3. 发明授权

EP2678444B1 ROTATION-DEPENDENT TRANSCRIPTIONAL SEQUENCING SYSTEMS AND METHODS OF USING 有权
标题翻译：旋转依赖性转录测序系统及其使用方法
公开(公告)号：EP2678444B1
公开(公告)日：2016-02-17
申请号：EP12709419.1
申请日：2012-02-23
申请人： Eve Biomedical, Inc.
发明人： KOTSEROGLOU, Theofilos
IPC分类号： C12Q1/68 , B01L3/00
CPC分类号： C12Q1/6869 , C12Q1/6874 , C12Q2521/119 , C12Q2521/543 , C12Q2523/303 , C12Q2563/143 , C12Q2565/601

4. 发明公开

EP2689028A4 ISOLATION OF POLYMERASE-NUCLEIC ACID COMPLEXES AND LOADING ONTO SUBSTRATES 有权转让
标题翻译：聚合酶核酸复合物的分离及其在底物上的加载
公开(公告)号：EP2689028A4
公开(公告)日：2014-09-17
申请号：EP12761363
申请日：2012-03-20
申请人： PACIFIC BIOSCIENCES CALIFORNIA
发明人： THANG PHAM , GRAY JEREMY , FEHR ADRIAN , BHAMIDIPATI ARUNASHREE , CHMURA AJ , TRAVERS KEVIN , OLIVARES ERIC , TSAI YU-CHIH
IPC分类号： C12Q1/48 , C12N15/11 , C12Q1/68 , G01N27/26 , G01N33/68
CPC分类号： C12Q1/6874 , B01L9/523 , C12Q1/6869 , C40B60/14 , G01N33/54313 , G01N33/54353 , G01N2333/922 , C12Q2521/543 , C12Q2523/303 , C12Q2525/301

5. 发明授权

EP2256188B1 METHOD FOR INCREASING ENZYMATIC REACTIVITY 有权
标题翻译：法提高酶促反应性
公开(公告)号：EP2256188B1
公开(公告)日：2014-06-18
申请号：EP09714443.0
申请日：2009-02-27
申请人： Riken , Dnaform
发明人： KOJIMA, Miki , CARNINCI, Piero , HAYASHIZAKI, Yoshihide , HARBERS, Matthias
IPC分类号： C12N9/00 , C12N15/09 , C12Q1/68
CPC分类号： C12N9/96 , C12Q1/6834 , C12Q1/686 , C12Q1/6869 , C12Q2527/127 , C12Q2527/125 , C12Q2521/543

6. 发明公开

EP2689028A2 ISOLATION OF POLYMERASE-NUCLEIC ACID COMPLEXES AND LOADING ONTO SUBSTRATES 有权转让
标题翻译：核酸聚合酶复合体的分离和他们的货物在基板上
公开(公告)号：EP2689028A2
公开(公告)日：2014-01-29
申请号：EP12761363.6
申请日：2012-03-20
申请人： Pacific Biosciences Of California, Inc.
发明人： THANG, Pham , GRAY, Jeremy , FEHR, Adrian , BHAMIDIPATI, Arunashree , CHMURA, Aj , TRAVERS, Kevin , OLIVARES, Eric , TSAI, Yu-Chih
IPC分类号： C12Q1/48 , C12Q1/68 , C12N15/11 , G01N27/26 , G01N33/68
CPC分类号： C12Q1/6874 , B01L9/523 , C12Q1/6869 , C40B60/14 , G01N33/54313 , G01N33/54353 , G01N2333/922 , C12Q2521/543 , C12Q2523/303 , C12Q2525/301
摘要： Disclosed are compositions, devices, and methods for loading molecules of interest onto a substrate by contacting beads having molecules of interest attached to them with the substrate, for example by providing a field that brings the beads into proximity or contact with the substrate and moves the beads with respect to the substrate. Such molecules of interest can be deposited onto substrates for single-molecule analysis.

7. 发明公开

EP2657330A1 DETECTION TOOL, AND DETECTION SYSTEM 审中-公开
标题翻译： DETEKTIONSINSTRUMENT UND DETEKTIONSSYSTEM
公开(公告)号：EP2657330A1
公开(公告)日：2013-10-30
申请号：EP11851713.5
申请日：2011-12-19
申请人： NEC Soft, Ltd.
发明人： WAGA Iwao
IPC分类号： C12M1/00 , C12M1/34 , G01N21/78 , C12N15/115
CPC分类号： G01N33/5302 , B01L3/5023 , B01L3/545 , B01L2300/021 , B01L2300/0627 , C12Q1/6823 , C12Q1/6837 , G01N21/78 , G01N33/56911 , G01N33/56983 , G01N33/581 , C12Q2521/543 , C12Q2525/161 , C12Q2525/205 , C12Q2563/125 , C12Q2565/513
摘要： The present invention provides a detection instrument capable of easily detecting an intended detection object without any skilled technique. The detection instrument (1) of the present invention includes a detection portion (12), a detection reagent which develops a color by specifically reacting with a detection object in a sample is placed in the detection portion (12), positional information of the detection reagent in the detection portion (12) is information on the detection object, and color development of the detection reagent can be optically read. It is preferred that a bar code is formed in the detection portion (12), and the detection reagent is placed as a part of the bar code.
摘要翻译：本发明提供一种能够容易地检测预期检测对象的检测仪器，而无需任何技术上的技术。本发明的检测仪器（1）包括检测部（12），通过与试样中的检测对象特异性反应而形成颜色的检测试剂被放置在检测部（12）中，检测位置信息检测部（12）中的试剂是关于检测对象的信息，可以光学地读取检测试剂的显色性。优选地，在检测部分（12）中形成条形码，并且将检测试剂置于条形码的一部分。

8. 发明公开

EP2267165A3 Nucleic acid sequence analysis 有权转让
公开(公告)号：EP2267165A3
公开(公告)日：2012-10-10
申请号：EP10182026.4
申请日：1998-07-24
申请人： GEN-PROBE INCORPORATED
发明人： Densham, Daniel
IPC分类号： C12Q1/68 , G01N21/00 , G01R33/00 , C07H19/06 , C07H19/16
CPC分类号： C12Q1/6825 , C12Q1/6869 , C12Q2565/628 , C12Q2521/543 , C12Q2521/101 , C12Q2565/633 , C12Q2525/186 , C12Q2523/319
摘要： The invention relates to the use of a nucleotide in a method for sequencing a polynucleotide, wherein the nucleotide comprises a blocking group at the 3' position and at the terminal phosphate group of the triphosphate chain, wherein the blocking groups are removable by monochromatic light of different wavelengths.

9. 发明授权

EP1923472B1 Detection of nucleic acid reactions on bead arrays 有权
标题翻译：检测珠阵列上的核酸反应
公开(公告)号：EP1923472B1
公开(公告)日：2012-04-11
申请号：EP07120734.4
申请日：2000-04-20
申请人： Illumina, Inc.
发明人： Gunderson, Kevin , Stuelpnagel, John, R. , Chee, Mark, S.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , B01J2219/005 , B01J2219/00596 , B01J2219/00648 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , C12Q1/6813 , C12Q1/6834 , C12Q1/6869 , C40B40/06 , C12Q2565/301 , C12Q2533/101 , C12Q2521/543 , C12Q2533/107 , C12Q2525/161 , C12Q2561/125 , C12Q2565/518 , C12Q2565/607 , C12Q2537/143

10. 发明公开

EP2365093A2 Method for sequencing nucleic acid molecules 有权
标题翻译： Verfahren zur Sequenzierung vonNukleinsäuremolekülen
公开(公告)号：EP2365093A2
公开(公告)日：2011-09-14
申请号：EP10166075.1
申请日：2000-05-18
申请人： CORNELL RESEARCH FOUNDATION, INC.
发明人： Korlach, Jonas , Webb, Watt W. , Levene, Michael , Turner, Stephen , Craighead, Harold G , Foquet, Mathieu
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6869 , Y10S436/80 , Y10S436/805 , Y10T436/143333 , C12Q2565/537 , C12Q2537/149 , C12Q2561/113 , C12Q2521/543 , C12Q2565/518 , C12Q2561/12
摘要： The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e., the activity of a nucleic acid polymerizing enzyme on the templage nucleic acid molecule to be sequenced is followed in real-time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonucleotide primer at an active site. A plurality of labelled types of nucleotide analogs are provided proximate to the active site, with each distinguishable type of nucleotide analog being complementary to a different nucleotide in the target nucleic acid sequence. The growing nucleic acid strand is extended by using the polymerase to add a nucleotide analog to the nucleic acid strand at the active site, where the nucleotide analog being added is complementary to the nucleotide of the target nucleic acid at the active site. The nucleotide analog added to the oligonucleotide primer as a result of the polymerizing step is identified. The steps of providing labelled nucleotide analogs, polymerizing the growing nucleic acid strand, and identifying the added nucleotide analog are repeated so that the nucleic acid strand is further extended and the sequence of the target nucleic acid is determined.
摘要翻译：本发明涉及对具有多个碱基的靶核酸分子进行测序的方法。在其原理中，聚合反应中碱添加的时间顺序是在核酸分子上测量的，即核酸聚合酶对待测序的模板核酸分子的活性是实时的。通过在碱添加序列的每个步骤中通过核酸聚合酶的催化活性鉴定哪个碱基被掺入目标核酸的生长互补链中来推断该序列。提供靶核酸分子复合物上的聚合酶在适于沿靶核酸分子移动的位置并在活性位点延伸寡核苷酸引物。在活性位点附近提供多个标记类型的核苷酸类似物，每个可区分类型的核苷酸类似物与靶核酸序列中的不同核苷酸互补。通过使用聚合酶来扩增生长的核酸链，在活性位点向核酸链添加核苷酸类似物，其中加入的核苷酸类似物与活性位点处的靶核酸的核苷酸互补。鉴定作为聚合步骤的结果添加到寡核苷酸引物中的核苷酸类似物。重复提供标记核苷酸类似物，聚合生长核酸链和鉴定添加的核苷酸类似物的步骤，使得核酸链进一步延长并确定靶核酸序列。

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