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1. 发明公开

EP3228698A4 HIGHLY EFFICIENT ETHANOL-FERMENTATIVE BACTERIA 失效
公开(公告)号：EP3228698A4
公开(公告)日：2018-05-23
申请号：EP14907566
申请日：2014-12-05
申请人： HONDA MOTOR CO LTD
发明人： TSUCHIDA YOSHIKI , KURIHARA IKUMI , IMAI TOMOHIRO , KOIKE IKU
IPC分类号： C12N1/15 , C12N15/09 , C12P7/06 , C12R1/84
CPC分类号： C12P7/06 , C12N1/16 , C12N15/01 , C12N15/04 , C12N15/09 , C12N15/905 , C12R1/645 , C12R1/84
摘要： A highly efficient ethanol-fermentative yeast having high efficiency in ethanol production is provided without introducing a foreign gene. The highly efficient ethanol-fermentative yeast features a fermentative yeast effectively producing ethanol from pentose and hexose and being deposited to NITE Patent Microorganisms Depositary under the accession number NITE BP-01963.

2. 发明公开

EP3290518A1 NOVEL GENE TARGETING METHOD 审中-公开
标题翻译：新型基因靶向方法
公开(公告)号：EP3290518A1
公开(公告)日：2018-03-07
申请号：EP16785983.4
申请日：2016-04-29
申请人： Hangzhou Genekine Biotech Co., Ltd
发明人： JIANG, Youwei , LI, Yunfei
IPC分类号： C12N15/11 , C12N1/16 , C12P21/00
CPC分类号： C12N15/905 , C12N1/16 , C12N9/1241 , C12N15/11 , C12N15/902 , C12N2800/30 , C12P21/00 , C12N15/102 , C12Q2521/507
摘要： Provided are a novel two-step gene targeting method and a nucleotide construct for gene targeting. The method can improve the gene targeting efficiency and accurately identify a target gene knock-out mutant. The method of the present invention comprises: firstly, efficiently replacing a target gene in a genome with a targeting box by homologous recombination, the targeting box consisting of a target gene activity variant, a marker gene and site-specific recombination sites; and secondly, resecting the targeting box by recombinase, leaving a site-specific recombination site on the target gene to generate a target gene knock-out mutant, and removing a recombinase expression vector from the knock-out mutant by using a counter selection marker in the recombinase expression vector.
摘要翻译：提供了一种新的两步基因靶向方法和用于基因靶向的核苷酸构建体。该方法可以提高基因靶向效率并准确鉴定靶基因敲除突变体。本发明的方法包括：首先通过同源重组，有效置换基因组中的靶基因，所述靶向盒由靶基因活性变异体，标记基因和位点特异性重组位点组成; 其次，通过重组酶切除靶向盒，在靶基因上留下位点特异性重组位点以产生靶基因敲除突变体，并通过使用中的计数选择标记从敲除突变体中去除重组酶表达载体重组酶表达载体。

3. 发明公开

EP3249045A1 METHODS FOR CLONING AND MANIPULATING GENOMES 审中-公开
标题翻译：克隆和操纵基因组的方法
公开(公告)号：EP3249045A1
公开(公告)日：2017-11-29
申请号：EP17177614.9
申请日：2010-03-05
申请人： Synthetic Genomics, Inc.
发明人： BENDERS, Gwynedd A , GLASS, John I , HUTCHISON, Clyde A , LARTIGUE, Carole , VASHEE, Sanjay , ALGIRE, Mikkel A , SMITH, Hamilton O , MERRYMAN, Charles E , NOSKOV, Vladimir N , CHUANG, Ray-Yuan , GIBSON, Daniel G , VENTER, J Craig
IPC分类号： C12N15/10
CPC分类号： C12N15/905 , C12N15/10 , C12N15/1031 , C12N15/1079 , C12N15/1093 , C12N15/81
摘要： Compositions and methods are disclosed herein for cloning a donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells.
摘要翻译：本文公开了用于在异源宿主细胞中克隆供体基因组的组合物和方法。在一个实施方案中，供体基因组可以在宿主细胞内进一步修饰。可以从宿主细胞中进一步分离修饰的或未修饰的基因组并将其转移至受体细胞。本文公开的方法可用于在更易处理的宿主细胞中改变来自难治性供体细胞的供体基因组。

4. 发明授权

EP2403944B1 METHODS FOR CLONING AND MANIPULATING GENOMES 有权
公开(公告)号：EP2403944B1
公开(公告)日：2017-06-28
申请号：EP10708854.4
申请日：2010-03-05
申请人： Synthetic Genomics, Inc.
发明人： BENDERS, Gwynedd, A. , GLASS, John, I. , HUTCHISON, Clyde, A. , LARTIGUE, Carole , VASHEE, Sanjay , ALGIRE, Mikkel, A. , SMITH, Hamilton, O. , MERRYMAN, Charles, E. , NOSKOV, Vladimir, N. , CHUANG, Ray-Yuan , GIBSON, Daniel G. , VENTER, J., Craig
IPC分类号： C12N15/10
CPC分类号： C12N15/905 , C12N15/10 , C12N15/1031 , C12N15/1079 , C12N15/1093 , C12N15/81

5. 发明授权

EP2650365B1 Rationally designed meganucleases with altered sequence specificity and DNA-binding affinity 有权
标题翻译：合理设计具有改变的序列特异性和DNA结合亲和力的大范围核酸酶
公开(公告)号：EP2650365B1
公开(公告)日：2016-09-14
申请号：EP13164564.0
申请日：2006-10-18
申请人： Precision Biosciences
发明人： Hellinga, Homme, W. , Smith, James, Jefferson , Jantz, Derek
IPC分类号： C12N9/22 , C12N15/90
CPC分类号： C12N9/22 , A61K38/465 , A61K48/00 , C12N15/8213 , C12N15/902 , C12N15/905 , C12N15/907
摘要： Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.

6. 发明授权

EP1953224B1 METHOD OF MODIFYING CHROMOSOME 有权转让
标题翻译：方法是用于变形的染色体
公开(公告)号：EP1953224B1
公开(公告)日：2013-03-27
申请号：EP06833655.1
申请日：2006-11-29
申请人： Asahi Glass Company, Limited
发明人： HIRASHIMA, Kyotaro , TOHDA, Hideki , HAMA, Yuko
IPC分类号： C12N15/00 , C12N15/09 , C12Q1/68
CPC分类号： C12N15/815 , C12N15/1079 , C12N15/1082 , C12N15/905

7. 发明授权

EP2357226B1 Rationally-designed meganucleases with altered sequence specificity and DNA-binding affinity 有权转让
标题翻译：合理设计的大范围核酸酶具有改变的序列特异性和DNA结合亲和力
公开(公告)号：EP2357226B1
公开(公告)日：2012-03-14
申请号：EP10191888.6
申请日：2006-10-18
申请人： Precision Biosciences
发明人： Hellinga, Homme W. , Smith, James Jefferson , Jantz, Derek
IPC分类号： C12N9/22 , C12N15/90
CPC分类号： C12N9/22 , A61K38/465 , A61K48/00 , C12N15/8213 , C12N15/902 , C12N15/905 , C12N15/907
摘要： Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.

8. 发明授权

EP1474522B1 A METHOD FOR GENERATING ENGINEERED CELLS FOR LOCUS SPECIFIC GENE REGULATION AND ANALYSIS 有权转让
标题翻译：用于生产操纵细胞基因座特异性基因调控及分析
公开(公告)号：EP1474522B1
公开(公告)日：2012-03-07
申请号：EP03703842.9
申请日：2003-01-17
申请人： Morphotek, Inc.
发明人： GRASSO, Luigi , KLINE, J., Bradford , NICOLAIDES, Nicholas, C. , SASS, Philip, M.
IPC分类号： C12N15/87 , C12N15/63 , C12N1/20 , C12N15/00 , C12Q1/68 , C07H21/04
CPC分类号： C12N15/905 , C12N15/102 , C12N15/902 , C12N15/907
摘要： Inhibitors of mismatch repair can be used to generate hypermutable cells and organisms. By inhibiting this process in cells, new cell lines and varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of homologous recombination. These methods are useful for generating targeted loci that can alter the expression profiles of target genes as well as tag exons of gene with a reporter marker to facilitate the monitoring of a given gene product when the host is grown under different conditions or exposed to biological and chemical entities.

9. 发明公开

EP2327774A2 Rationally-designed meganucleases with altered sequence specificity and DNA-binding affinity 审中-公开
标题翻译：理性设计的大范围核酸酶具有改变的序列特异性和DNA结合亲和力
公开(公告)号：EP2327774A2
公开(公告)日：2011-06-01
申请号：EP10191885.2
申请日：2006-10-18
申请人： Precision Biosciences
发明人： Hellinga, Homme W. , Smith, James Jefferson , Jantz, Derek
IPC分类号： C12N9/22 , C12N15/90
CPC分类号： C12N9/22 , A61K38/465 , A61K48/00 , C12N15/8213 , C12N15/902 , C12N15/905 , C12N15/907
摘要： Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.

10. 发明授权

EP2004827B1 IMPROVED METHOD FOR HOMOLOGOUS RECOMBINATION IN FUNGAL CELLS 有权
标题翻译：改进方法同源重组蘑菇细胞
公开(公告)号：EP2004827B1
公开(公告)日：2010-03-10
申请号：EP07726886.0
申请日：2007-03-14
申请人： DSM IP Assets B.V.
发明人： BERG, VAN DEN, Marco Alexander , OFFRINGA, Remko , BOER, DE, Paulo
IPC分类号： C12N15/80 , C12N15/90
CPC分类号： C12N15/815 , C12N15/905 , C12N2800/30 , C12N2800/70

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