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    • 7. 发明公开
    • Method of separating living cells
    • Methode zur Abtrennung lebender Zellen。
    • EP0368621A2
    • 1990-05-16
    • EP89311517.0
    • 1989-11-07
    • HITACHI, LTD.
    • Ishida, MasahikoHaga, RyoichiMatsuzaki, Harumi
    • B04B5/00B04B15/12C12M1/00C12N1/00C12M3/00
    • B04B5/00B04B15/12C12M47/02C12N1/02Y10T436/25375
    • A centrifugal separation method for living cells is disclosed in which a cell containing solution is charged in a rotor chamber confined by a steam pressure-resistant wall. A rotor having septa is disposed in the rotor chamber. The septa are rotated in the same direction at the same angular velocity as the rotor while at least part thereof is immersed in a solution in the rotor when cells are to be centrifuged from the cell-containing solution. After the cells are separated and adhered to the inner surface of the rotor, a supernatant in the rotor is discharged from the rotor. Then a fresh cultivating medium is introduced into the rotor, and the septa and the rotor are rotated at mutually different angular velocities while at least part of the septa is immersed in the fresh medium in the rotor. As a result, the cells are again suspended in the fresh medium.
    • 公开了一种用于活细胞的离心分离方法,其中将含细胞的溶液装入由耐蒸汽压壁限制的转子室中。 具有隔片的转子设置在转子室中。 隔片以与转子相同的角速度沿相同的方向旋转,而当细胞从含细胞溶液离心时,其至少一部分浸入到转子中的溶液中。 在细胞分离并粘附到转子的内表面之后,转子中的上清液从转子中排出。 然后将新鲜的栽培培养基引入转子中,并且隔片和转子以相互不同的角速度旋转,同时至少部分隔片浸入转子中的新鲜培养基中。 结果,细胞再次悬浮在新鲜培养基中。