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1. 发明公开

EP3050886A1 Fluorescent dyes and dye precursors 审中-公开
标题翻译： Fluoreszenzfarbstoffe undFarbstoffvorläufer
公开(公告)号：EP3050886A1
公开(公告)日：2016-08-03
申请号：EP15153463.3
申请日：2015-02-02
申请人： Bürkert Werke GmbH
发明人： Lelant, Célia , Bonnet, Dominique , Galzi, Jean-Luc
IPC分类号： C07D493/10 , A61K31/35 , A61K31/365 , C09B11/06 , C09K11/06 , C09K11/59 , C09K11/66 , C12N1/00 , C12Q1/02 , G01N33/48 , G01N33/50 , G01N21/64 , G01N33/52
CPC分类号： C07D493/10 , C09B11/06 , C09B11/08 , C09K11/06 , C12Q1/02 , G01N1/30 , G01N33/48 , G01N33/50 , G01N33/5091 , G01N33/582
摘要： A xanthene compound is used for discriminating living from non-living unicellular and multicellular organisms. The xanthene compound is represented by the following formula (I)

wherein
each X is, independently from each other, one of H, F, Cl, Br, I, CN;
Z is one of O, Si, Ge or Sn;
R 1 is hydrogen, -(C=O)-R 4 or -CH 2 -O-(C=O)-R 4 , wherein R 4 is substituted or unsubstituted alkyl having 1 to 18 carbon atoms, substituted or unsubstituted alkenyl having 2 to 18 carbon atoms, or substituted or unsubstituted aralkyl having at least 7 carbon atoms, or wherein R 1 is a residue derived from an amino acid, peptide, nucleotide or oligonucleotide, monosaccaride or polysaccharide;
A is -NH 2 , -OR 1 or -NR 5 (C=O)-R 4 wherein R 4 independently has the meaning as defined above and R 5 is H, substituted or unsubstituted alkyl having 1 to 18 carbon atoms, substituted or unsubstituted alkenyl having 2 to 18 carbon atoms, or substituted or unsubstituted aralkyl having at least 7 carbon atoms;
R 2 and R 3 are, independently from each other, H or -(C=O)-NR 6 R 7 , wherein at least one of R 2 and R 3 is not H, and wherein R 6 and R 7 are selected from H, substituted or unsubstituted alkyl having 1 to 18 carbon atoms, substituted or unsubstituted alkenyl having 2 to 18 carbon atoms, or substituted or unsubstituted aralkyl having at least 7 carbon atoms.
摘要翻译：呫吨化合物用于区分生活与非生物单细胞和多细胞生物的生物。呫吨化合物由下式（I）表示，其中每个X彼此独立地为H，F，Cl，Br，I，CN之一; Z是O，Si，Ge或Sn之一; R 1是氢， - （C = O）-R 4或-CH 2 -O-（C = O）-R 4，其中R 4是具有1至18个碳原子的取代或未取代的烷基， 2至18个碳原子，或取代或未取代的具有至少7个碳原子的芳烷基，或其中R 1是衍生自氨基酸，肽，核苷酸或寡核苷酸，单糖或多糖的残基; A是-NH 2，-OR 1或-NR 5（C = O）-R 4，其中R 4独立地具有如上定义的含义，R 5是H，取代或未取代的具有1至18个碳原子的烷基，取代或具有2至18个碳原子的未取代的烯基，或具有至少7个碳原子的取代或未取代的芳烷基; R 2和R 3彼此独立地为H或 - （C = O）-NR 6 R 7，其中R 2和R 3中的至少一个不是H，并且其中R 6和R 7选自 H，取代或未取代的碳原子数1〜18的烷基，取代或未取代的碳原子数为2〜18的烯基或取代或未取代的碳数7以上的芳烷基。

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