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71. 发明公开

EP0970195A4 FATTY-ACID AMIDE HYDROLASE 失效
标题翻译：脂肪水解酶
公开(公告)号：EP0970195A4
公开(公告)日：2001-12-05
申请号：EP97948210
申请日：1997-11-04
申请人： SCRIPPS RESEARCH INST
发明人： GILULA NORTON B , CRAVATT BENJAMIN F , LERNER RICHARD A
IPC分类号： C12N15/00 , A61K31/12 , A61K38/46 , A61P25/00 , A61P25/20 , A61P43/00 , C07C45/45 , C07C49/227 , C07C57/03 , C07C233/09 , C07C327/22 , C07F9/54 , C12N9/80 , C12N9/99 , C12N15/09 , C12P7/64 , C12Q1/34 , C12N9/78 , C12N9/14
CPC分类号： C07C45/45 , C07C49/227 , C07C57/03 , C07C233/09 , C07C327/22 , C07F9/5407 , C12N9/80 , C12N9/99 , C12P7/6418
摘要： The soporific activity of cis-9,10-octadecenoamide and other soporific fatty-acid primary amides is neutralized by hydrolysis in the presence of fatty-acid amide hydrolase (FAAH). Hydrolysis of cis-9,10-octadecenoamide by FAAH leads to the formation of oleic acid, a compound without soporific activity. FAAH has been isolated and the gene encoding FAAH has been cloned, sequenced, and used to express recombinant FAAH. Inhibitors of FAAH are disclosed to block the hydrolase activity.

72. 发明公开

EP0920496A4 RECOMBINATION OF POLYNUCLEOTIDE SEQUENCES USING RANDOM OR DEFINED PRIMERS 失效
标题翻译：多核苷酸序列的重组使用任何偶然的或限定的底层涂料
公开(公告)号：EP0920496A4
公开(公告)日：2001-05-02
申请号：EP98915171
申请日：1998-03-25
申请人： CALIFORNIA INST OF TECHN
发明人： ARNOLD FRANCES H , SHAO ZHIXIN , AFFHOLTER JOSEPH A , ZHAO HUIMIN , GIVER LORRAINE J
IPC分类号： C12N15/09 , C12N9/16 , C12N9/56 , C12N9/80 , C12N15/10 , C12Q1/68 , C12P19/34
CPC分类号： C12N15/1027 , C12N9/16 , C12N9/54 , C12N9/80 , C12Q1/6811 , C12Q1/683 , C12Q2537/155 , C12Q2533/101 , C12Q2525/179 , C12Q2549/119
摘要： A method for in vitro mutagenesis and recombination of polynucleotide sequences based on polymerase-catalyzed extension of primer oligonucleotides is disclosed. The method involves priming template polynucleotide(s) with random-sequences or defined-sequence primers to generate a pool of short DNA fragments with a low level of point mutations. The DNA fragments are subjected to denaturization followed by annealing and further enzyme-catalyzed DNA polymerization. This procedure is repeated a sufficient number of times to produce full-length genes which comprise mutants of the original template polynucleotides. These genes can be further amplified by the polymerase chain reaction and cloned into a vector for expression of the encoded proteins.

73. 发明公开

EP0940409A4 PROCESS FOR THE PREPARATION OF SPHINGOLIPIDS AND SPHINGOLIPID DERIVATIVES 失效
标题翻译：用于生产鞘脂和神经鞘脂
公开(公告)号：EP0940409A4
公开(公告)日：2001-01-17
申请号：EP97930823
申请日：1997-07-17
申请人： TAKARA SHUZO CO
发明人： ITO MAKOTO , KURITA TOYOHISA , MITSUTAKE SUSUMU , KITA KATSUHIRO
IPC分类号： C12P13/00 , C07C231/02 , C07C233/18 , C07C233/20 , C07F9/10 , C07H15/04 , C12N9/80 , C12P13/02 , C12P19/44 , C07F9/09
CPC分类号： C07H15/04 , C07F9/10 , C12N9/80 , C12P13/02
摘要： A process for specifically preparing sphingolipids or sphingolipid derivatives wherein the long-chain fatty acid moiety bonded to a sphingoid is modified or substituted; and sphingolipids or sphingolipid derivatives prepared by the process. The process is characterized by enzymatically reacting a sphingolipid with an aliphatic carboxylic acid or the like in the presence of an enzyme specifically hydrolyzing the acid-amide linkage between the sphingoid and the fatty acid component constituting the sphingolipid to give another sphingolipid or sphingolipid derivative different from the original one in the aliphatic acid chain.

74. 发明公开

EP0919616A1 METHODS FOR PREPARING BIOREACTORS 失效
标题翻译：用于生产生物反应器
公开(公告)号：EP0919616A1
公开(公告)日：1999-06-02
申请号：EP97933862.1
申请日：1997-07-30
申请人： FUJISAWA PHARMACEUTICAL CO., LTD.
发明人： ASAI, Katsushi
IPC分类号： C12N9/00 , C12N11/06 , C12N9/80
CPC分类号： C12N9/80 , C12N11/02 , C12N11/08 , Y10S435/816
摘要： Methods for purifying enzymes, in particular, acylases characterized by selectively aggregating and precipitating contaminating enzymes, in particular, deacetylases with the use of surfactants, in particular, cationic surfactants; methods for regenerating immobilized enzyme carriers, in particular, those made from synthetic absorbents or ion exchange resins by treating immobilized enzymes prepared by bonding the enzymes to the carriers optionally followed by cross-linking with proteases; methods for regenerating carriers as described above wherein the carriers are porous ones; and methods for regenerating carriers as described above wherein the enzyme is a cephalosporin C acylase. By the above methods for purifying enzymes, contaminating enzymes which are undesirable for the target enzymes but cannot be eliminated by the conventional separation techniques can be selectively eliminated. Thus, these methods are useful in the production of highly pure enzyme specimens. Compared with the conventional methods, the above-mentioned methods for regenerating immobilized enzyme carriers make it possible to very efficiently eliminate the enzymes from the carriers, in particular, porous ones through digestion with proteases. Therefore, the carriers can be used repeatedly, which makes these methods useful from the viewpoints of the environment and cost.

75. 发明授权

EP0707063B1 Glycolipid ceramide deacylase 失效
标题翻译：糖脂Ceramiddeacylase
公开(公告)号：EP0707063B1
公开(公告)日：1998-06-17
申请号：EP95111412.3
申请日：1995-07-20
申请人： TAKARA SHUZO CO. LTD.
发明人： Ito, Makoto , Kurita, Toyohisa, c/o Matsushima Haitsu , Kita, Katsuhiro
IPC分类号： C12N9/80 , C12P13/00 , C12P19/26
CPC分类号： C12P13/001 , C07H15/04 , C12N9/80 , C12N11/02 , C12P13/02 , C12P19/26

76. 发明公开

EP0790303A1 Novel creatine amidinohydrolase, production thereof and use thereof 失效
标题翻译： Neue Creatinamidohydrolase，ihre Herstellung und Verwendung
公开(公告)号：EP0790303A1
公开(公告)日：1997-08-20
申请号：EP97102270.2
申请日：1997-02-13
申请人： Toyo Boseki Kabushiki Kaisha
发明人： Sogabe, Atsushi, Toyo Boseki K.K. , Hattori, Takashi, Toyo Boseki K.K. , Nishiya, Yoshiaka, Toyo Boseki K.K. , Kawamura, Yoshihisa, Toyo Boseki K.K.
IPC分类号： C12N9/78 , C12N9/80 , C12Q1/34
CPC分类号： G01N33/52 , C12Q1/34 , G01N2333/978 , Y10S435/829
摘要： A creatine amidinohydrolase having the following physicochemical properties:

Action: catalyzing the following reaction; creatine + H 2 O → sarcosine + urea
Optimum temperature: about 40 - 50°C
Optimum pH: pH about 8.0 - 9.0
Heat stability: not more than about 50°C (pH 7.5, 30 min)
Km value for creatine in a coupling assay using a sarcosine oxidase and a peroxidase: about 3.5 - 10.0 mM
Molecular weight: about 43,000 (SDS-PAGE)
Isoelectric point: about 3.5,
a method for producing said enzyme, comprising culture of microorganism producing said enzyme, a method for the determination of creatine or creatinine in a sample using said enzyme, and a reagent therefor. According to the present invention, a creatine amidinohydrolase having a smaller Km value than that of the conventionally known creatine amidinohydrolase can be produced in an industrially large amount, and can be used as a routine reagent for clinical tests for determining creatine and creatinine in biological samples.
摘要翻译：具有以下物理化学性质的肌酸脒基水解酶：作用：催化以下反应; 肌酸+ H2O - >肌氨酸+尿素最佳温度：约40 - 50℃最佳pH：pH约8.0 - 9.0热稳定性：不超过约50℃（pH 7.5,30分钟）偶联试验中肌酸的Km值使用肌氨酸氧化酶和过氧化物酶：约3.5-10.0mM分子量：约43,000（SDS-PAGE）等电点：约3.5，一种生产所述酶的方法，包括培养产生所述酶的微生物，测定方法使用所述酶的样品中的肌酸或肌酸酐及其试剂。根据本发明，具有比常规已知的肌酸脒基水解酶更小的Km值的肌酸脒基水解酶可以在工业上大量生产，并且可以用作生物样品中肌酸和肌酸酐测定的临床试验的常规试剂。

77. 发明公开

EP0758020A2 The use of homologous amdS genes as selectable markers 失效
标题翻译： amdS-Gen aus Aspergillus nigerfüreine Acetamidase kodierend
公开(公告)号：EP0758020A2
公开(公告)日：1997-02-12
申请号：EP96202188.7
申请日：1996-08-05
申请人： GIST-BROCADES B.V.
发明人： Swinkels, Bart Willem , Selten, Gerardus Cornelis Maria , Bakhuis, Janna Gardina , Bovenberg, Roelof Ary Lans , Vollebregt, Adrianus Wilhelmus Hermanus
IPC分类号： C12N15/52 , C12N9/80 , C12N1/15 , C12N1/19 , C12N15/90
CPC分类号： C12N9/80
摘要： The present invention discloses novel amd S genes from fungi previously not known to contain an amd S gene, such as Aspergillus niger and Penicillium chrysogenum. The novel amd S genes can be used as homologous selectable marker genes in the transformation of these fungi. Alternatively, the cloned amd S genes can be used to inactivate the endogenous copy of the gene in order to reduce the background in transformation experiments.
摘要翻译：本发明公开了以前不知道含有amdS基因的真菌的新型amdS基因，如黑曲霉和产黄青霉。该新型amdS基因可用作这些真菌转化中的同源选择标记基因。或者，克隆的amdS基因可用于灭活基因的内源拷贝，以减少转化实验中的背景。

78. 发明授权

EP0444639B1 A gene encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant 失效
标题翻译：编码具有多肽腈水合酶活性的基因，所述含转化子的基因，和用于制备该转化体的酰胺的方法。
公开(公告)号：EP0444639B1
公开(公告)日：1996-01-31
申请号：EP91102936.1
申请日：1991-02-27
申请人： NITTO CHEMICAL INDUSTRY CO., LTD. , Beppu, Teruhiko , YAMADA, Hideaki
发明人： Beppu, Teruhiko , Yamada, Hideaki , Nagasawa, Toru , Horinouchi, Sueharu , Nishiyama, Makoto
IPC分类号： C12N15/55 , C12N9/80 , C12P13/02
CPC分类号： C12N9/80 , C12P13/02

79. 发明公开

EP0614972A2 N-Acetyl-D-glucosamine deacetylase and a process for preparing the same 失效
标题翻译： N-乙酰基-D-葡萄糖胺脱乙酰酶和Verfahren zu deren Herstellung。
公开(公告)号：EP0614972A2
公开(公告)日：1994-09-14
申请号：EP94301768.1
申请日：1994-03-11
申请人： AGENCY OF INDUSTRIAL SCIENCE AND TECHNOLOGY
发明人： Yamano, Naoko , Tanaka, Ryutarou , Fujishima, Shizu
IPC分类号： C12N9/80 , C12P19/26
CPC分类号： C12P19/26 , C12N9/80
摘要： There are provided N-acetyl-D-glucosamine deacetylase capable of hydrolyzing acetamide group of N-acetyl-D-glucosamine to produce D-glucosamine and acetic acid, which has the following physicochemical properties :

(1) Substrate specificity : N-acetyl-D-glucosamine monomer, not the oligomer or polymer thereof ;
(2) Optimal pH : 7.8-8.2 at 37 °C;
(3) Stable pH : 6.0-9.0 at 45 °C ;
(4) Optimal temperature : 28-39 °C ;
(5) Molecular weight : 70,000-200,000 as determined using TSK-gel G-3000 SW column,

and a process for preparing the same using a microorganism belonging to Vibrio sp.
摘要翻译：提供能够水解N-乙酰基-D-葡糖胺的乙酰胺基团以产生D-葡糖胺和乙酸的N-乙酰基-D-葡糖胺脱乙酰酶，其具有以下物理化学性质：（1）底物特异性：N-乙酰基 - D-葡糖胺单体，而不是其低聚物或聚合物; （2）最适pH：7.8-8.2，37℃; （3）在45℃下稳定的pH：6.0-9.0; （4）最佳温度：28-39℃; （5）使用TSK-gel G-3000 SW柱测定的分子量：70,000-200,000，以及使用属于弧菌属微生物的微生物制备该方法。

80. 发明授权

EP0329324B1 Polymeric compositions and their production 有权
标题翻译：聚合物组合物和它们的制备。
公开(公告)号：EP0329324B1
公开(公告)日：1994-04-20
申请号：EP89301185.8
申请日：1989-02-08
申请人： ALLIED COLLOIDS LIMITED
发明人： Farrar, David , Flesher, Peter , Lawrence, Peter Robin Broughton
IPC分类号： C08L33/26 , C08F6/00 , C12N9/80
CPC分类号： C12N9/80 , C08F6/006 , C08L33/26

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