专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明公开

EP3189158A1 NUCLEIC ACID ANALYSIS 审中-公开
标题翻译：核酸分析
公开(公告)号：EP3189158A1
公开(公告)日：2017-07-12
申请号：EP15777986.9
申请日：2015-09-04
申请人： REVOLUGEN LIMITED
发明人： MINTER, Stephen, John , PATSOS, Georgios
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6823 , C12Q1/6818 , C12Q1/6827 , C12Q1/6897 , C12Q2521/319 , C12Q2537/155 , C12Q2563/107 , C12Q2565/1015
摘要： A method of analysing for a single stranded nucleic acid present, or potentially present, in a sample comprises a step in which the target nucleic acid (if present in the sample) displaces—and hybridises to—a reporter strand originally present in an interrogating duplex structure comprised of the reporter strand and a displaceable shorter strand. The reporter strand is tagged at or adjacent one end thereof with a reporter moiety capable of providing a detectable signal. The reporter/target duplex structure is such that the reporter strand may be selectively enzymatically digested (e.g. by means of λ-exonuclease) from its end opposite the reporter moiety to release that moiety for direct or indirect detection and regenerate single stranded target, which may then cycle through a plurality of the displacement and digestion steps to result in amplification of signal.
摘要翻译：分析样品中存在或可能存在的单链核酸的方法包括以下步骤，其中靶核酸（如果存在于样品中）替代并杂交至最初存在于询问双链体中的报道链结构由报道链和可移位的较短链组成。报道链在其一端或其附近被标记有能够提供可检测信号的报道分子部分。报道子/靶双链体结构使得报道链可以从其报道部分对面的末端选择性酶促消化（例如借助于λ-外切核酸酶）以释放该部分以用于直接或间接检测并且再生单链靶，其可以然后循环经过多个置换和消化步骤以导致信号的放大。

2. 发明授权

EP2633069B1 SEQUENCING METHODS 有权转让
标题翻译：测序方法
公开(公告)号：EP2633069B1
公开(公告)日：2015-07-01
申请号：EP11776423.3
申请日：2011-10-26
申请人： Illumina, Inc.
发明人： RIGATTI, Roberto , BOUTELL, Jonathan Mark , BETLEY, Jason Richard , GORMLEY, Niall Anthony , RONAGHI, Mostafa , EVERS, Dirk
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q2565/514 , C12Q2537/155 , C12Q2525/186 , C12Q2525/185 , C12Q2537/113 , C12Q2525/204

3. 发明公开

EP2633069A1 SEQUENCING METHODS 有权转让
标题翻译：测序方法
公开(公告)号：EP2633069A1
公开(公告)日：2013-09-04
申请号：EP11776423.3
申请日：2011-10-26
申请人： Illumina, Inc.
发明人： RIGATTI, Roberto , BOUTELL, Jonathan Mark , BETLEY, Jason Richard , GORMLEY, Niall Anthony , RONAGHI, Mostafa , EVERS, Dirk
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q2565/514 , C12Q2537/155 , C12Q2525/186 , C12Q2525/185 , C12Q2537/113 , C12Q2525/204
摘要： The present technology relates to molecular sciences, such as genomics. More particularly, the present technology relates to nucleic acid sequencing.
摘要翻译：本技术涉及分子科学，例如基因组学。更具体地说，本技术涉及核酸测序。

4. 发明公开

EP1948823A4 TARGET NUCLEIC ACID SIGNAL DETECTION 审中-公开
标题翻译： ZIELNUKLEINSÄURESIGNALNACHWEIS
公开(公告)号：EP1948823A4
公开(公告)日：2010-01-27
申请号：EP06825860
申请日：2006-10-11
申请人： STRATAGENE CALIFORNIA
发明人： SORGE JOSEPH A , CARSTENS CARSTEN-PETER , MONELL CRAIG ROBERT , BELYAEV ALEXANDER
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q1/6844 , C12Q2537/155 , C12Q2521/101 , C12Q2525/307 , C12Q2521/501 , C12Q2525/155

5. 发明公开

EP1929045A1 METHODS, COMPOSITIONS AND KITS FOR ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS 失效
标题翻译：方法，组合物和试剂盒的核酸等温扩增
公开(公告)号：EP1929045A1
公开(公告)日：2008-06-11
申请号：EP06803026.1
申请日：2006-09-06
申请人： GEN-PROBE INCORPORATED
发明人： BECKER, Michael, M. , LIVEZEY, Kristin, W.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12P19/34 , C12Q1/6853 , C12Q2527/101 , C12Q2537/155 , C12Q2525/161 , C12Q2525/179 , C12Q2537/1376
摘要： Methods and compositions are described for isothermal nucleic acid amplification of a nucleic acid template strand by using an oligonucleotide primer that includes an AT-rich nucleotide sequence and a polymerase having strand displacement activity.

6. 发明公开

EP0708840A1 IMPROVED METHODS FOR DETECTING NUCLEIC ACID SEQUENCES 失效
标题翻译：改进方法用于确定NUCLEIC序列
公开(公告)号：EP0708840A1
公开(公告)日：1996-05-01
申请号：EP94921315.0
申请日：1994-06-16
申请人： THE RESEARCH FOUNDATION OF STATE UNIVERSITY OF NEW YORK
发明人： LANE, Michael J. , BENIGHT, Albert S. , FALDASZ, Brian D.
IPC分类号： C12N15 , C12Q1
CPC分类号： C12Q1/6813 , C12Q2537/155 , C12Q2525/121 , C12Q2521/307
摘要： Improved cycling probe reactions are disclosed.

7. 发明公开

EP0599338A3 Method for detecting target nucleic acid 失效
标题翻译：一种用于检测靶核酸的方法。
公开(公告)号：EP0599338A3
公开(公告)日：1995-06-21
申请号：EP93119104.3
申请日：1993-11-26
申请人： CANON KABUSHIKI KAISHA
发明人： Okamoto, Tadashi, c/o Canon Kabushiki Kaisha , Tomida, Yoshinori, c/o Canon Kabushiki Kaisha , Yamamoto, Nobuko, c/o Canon Kabushiki Kaisha , Kawaguchi, Masahiro, c/o Canon Kabushiki Kaisha , Makino, Keisuke , Murakami, Akira
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , Y10S435/81 , C12Q2563/173 , C12Q2527/107 , C12Q2565/107 , C12Q2563/113 , C12Q2537/155
摘要： A method for detecting a target nucleic acid comprises the steps of reacting a sample with a probe in the presence of two or more kinds of reagents capable of being made an irreversible change capable of being detected and accumulating by an interaction through a double helix structure under a condition enabling the replication of the formation and dissociation of a hybrid composed of the target nucleic acid in the sample and the probe, accumulating the irreversible change caused by the interaction of the reagents, and then detecting the accumulated change.

8. 发明公开

EP0502180A4 ENZYMATIC SYNTHESIS OF OLIGONUCLEOTIDES 失效
标题翻译：寡核苷酸的酶合成
公开(公告)号：EP0502180A4
公开(公告)日：1993-03-17
申请号：EP91919119
申请日：1991-09-23
申请人： AMGEN INC.
发明人： RICHARDS, RODNEY, M.
IPC分类号： C12N15/09 , A61K20060101 , A61K31/70 , C07H20060101 , C07H1/00 , C07H15/12 , C07H21/00 , C12N15/10 , C12P20060101 , C12P19/34 , C12Q20060101 , C12Q1/68 , G01N20060101 , G01N33/566 , G01N33/58
CPC分类号： C12N15/10 , C12Q1/682 , C12Q1/6853 , Y02P20/582 , C12Q2537/155 , C12Q2525/301 , C12Q2525/131 , C12Q2533/101 , C12Q2533/107

9. 发明公开

EP0300796A3 Amplification method for polynucleotide assays 失效转让
标题翻译：聚核苷酸测定法的放大方法多核苷酸测定的放大方法
公开(公告)号：EP0300796A3
公开(公告)日：1989-07-19
申请号：EP88306717.5
申请日：1988-07-21
申请人： SYNTEX (U.S.A.) INC.
发明人： Becker, Martin , Goodman, Thomas , Rose, Samual , Ullman, Edwin F.
IPC分类号： C12Q1/68 , C07H21/00 , C12P19/34
CPC分类号： C12Q1/701 , C12Q1/6813 , C12Q1/683 , C12Q1/6844 , C12Q1/6853 , C12Q1/6858 , Y10T436/143333 , C12Q2565/537 , C12Q2537/155 , C12Q2521/301
摘要： A method is disclosed for producing multiple copies of a primary polynucleotide sequence located at the 3′ terminus of a polynucleotide. The method comprises (a) forming in the presence of nucleoside triphosphates and template-dependent polynucleotide polymerase an extension of a primary polynucleotide sequence hybridized with a template sequence of a single stranded pattern polynucleotide comprising two or more template sequences each containing one or more cleavable sites (b) cleaving into fragments said extension at cleavable sites in the presence of means for specifically cleaving said cleavable sites when said extension is hybridized with said template sequence, (c) dissociating said fragments, (d) hybridizing said fragments with single stranded pattern polynucleotide, and repeating steps (a)-(d). Steps (a)-(d) may be conducted simultaneously or wholly or partially sequentially. The method may be applied in the detection of a polynucleotide analyte in a sample suspected of containing such analyte to facilitate such detection. Also disclosed are compositions for conducting the method of the invention.

10. 发明公开

EP3265587A1 HOMOGENOUS ENTROPY-DRIVEN BIOMOLECULAR ASSAY (HEBA) 审中-公开
标题翻译：同源化熵驱动生物分子测定（HEBA）
公开(公告)号：EP3265587A1
公开(公告)日：2018-01-10
申请号：EP16759399.5
申请日：2016-03-02
申请人： The Regents of the University of California
发明人： DI CARLO, Dino , KIM, Donghyuk
IPC分类号： C12Q1/68
CPC分类号： C12Q1/682 , C12Q1/68 , C12Q1/6804 , G01N21/6428 , G01N2021/6432 , G01N2021/6439 , C12Q2563/107 , C12Q2563/125 , C12Q2563/159 , C12Q2565/30 , C12Q2565/619 , C12Q2525/205 , C12Q2563/179 , C12Q2537/137 , C12Q2537/155 , C12Q2537/157
摘要： A method for detecting an analyte in a sample includes the steps of binding a first catalytic precursor to the analyte at a first epitope and binding a second catalytic precursor to the analyte at a second, different epitope to generate a catalytic complex. The catalytic complex is reacted with a multiplex molecular substrate to generate a first target molecule and an intermediate substrate containing the bound catalytic complex. The intermediate substrate is reacted with a dummy reactant to generate a second target molecule, wherein the reaction further generates a waste molecule containing the dummy reactant and a free catalytic complex. An optical signal that is generated by one or more dye(s) specific to the first target molecule and/or the second target molecule is detected to detect the presence of the analyte in the sample. The overall reaction has substantially net zero enthalpy and a positive entropy change.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式